NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 11321R-3

Symbol CG11321 Full Name CG11321

CG No CG11321 Old CG No CG11321

Synonyms CG11321

Accession No (Link to NCBI) NM_135228.1

Inserted Chr. lll

Insertional Mutation lethal

Phenotype induced by Act5C-GAL4 at 28 degrees semi-lethal

Map Viewer

Reference
[Please submit your publication] Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Du J, Zhang J, Su Y, Liu M, Ospina JK, Yang S, Zhu AJ.
In vivo RNAi screen reveals neddylation genes as novel regulators of Hedgehog signaling.
PLoS One (2011) 6(9) e24168 [ PubMed ID = 21931660 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgTACCGCCAACAGAAGGCTCGC

Primer Seq. 3'

aatctagaggtaccGCAGCTCTGGAGGCAGCTGAA

Predicted Fragment Size

IR fragment full Seq

0001 TACCGCCAAC AGAAGGCTCG CCAGGATCTG AGCGCCAACA GCGAACAGGC GGCCGGCGcC 
0061 CAGCGGCGAC AGATGAGCAC ATCGGTGTTC AATCTCAACT CGAATCCACG TCGACCAACT 
0121 GTCCGAGGCC CAAAACGGAG GTGCCTGGCN TCGCCAATCA GCGCATCCAG CAGGCCCAAT 
0181 TCCCTGGCGC AGCTAAACTG CGCTGGATGT CAACAGAGTC AGCAGCATCC TGGCTGGGCA 
0241 NCAgCATCCG CACCAAGCAC TTCCTCAGCA CCAGCATCCC GATCAGTGGT CACAGTTCGG 
0301 CTCCCAACAG CAATTCAACA ACTCCAATCT GTCCCTGAAC GTTGGACCGG GTTACATGTC 
0361 GCAGCAACAT CATCCACACT ATCCGCCACC AGTGTTTATG ACCCAGCGTG GTATGATGCC 
0421 CAATGTGTAT CCAGGAGCAC CAGGCTATCC CATGATGCAT CCAGGTGTCA TGGGAATGCC 
0481 ACCTTCAGCT GCCTCCAGAG CTGC

in silico PCR Fragment

0001 TACCGCCAAC AGAAGGCTCG CCAGGATCTG AGCGCCAACA GCGAACAGGC GGCCGGCGCC 
0061 CAGCGGCGAC AGATGAGCAC ATCGGTGTTC AATCTCAACT CGAATCCACG TCGACC-ACT 
0121 GTCCGAGGCC CAAAACGGAG GTGCCTGGC- TCGCCAATCA GCGCATCCAG CAGGCCCAA- 
0181 TCCCTGGCGC AGCTAAACTG CGCTGGATGT CAACAGAGTC AGCAGCATCC TGGCTGGGCA 
0241 -CAGCATCCG CACCAAGCAC TTCCTCAGCA CCAGCATCCC GATCAGTGGT CACAGTTCGG 
0301 CTCCCAACAG CAATTCAACA ACTCCAATCT GTCCCTGAAC GTTGGACCGG GTTACATGTC 
0361 GCAGCAACAT CATCCACACT ATCCGCCACC AGTGTTTATG ACCCAGCGTG GTATGATGCC 
0421 CAATGTGTAT CCAGGAGCAC CAGGCTATCC CATGATGCAT CCAGGTGTCA TGGGAATGCC 
0481 ACCTTCAGCT GCCTCCAGAG CTGC

Assemble Data


11321R-3.IR_full       1   TACCGCCAACAGAAGGCTCGCCAGGATCTGAGCGCCAACAGCGAACAGGCGGCCGGCGCC 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11321R-3.in silico     1   TACCGCCAACAGAAGGCTCGCCAGGATCTGAGCGCCAACAGCGAACAGGCGGCCGGCGCC 60


11321R-3.IR_full       61  CAGCGGCGACAGATGAGCACATCGGTGTTCAATCTCAACTCGAATCCACGTCGACCAACT 120
                           |||||||||||||||||||||||||||||||||||||||||||||||||||||||| |||
11321R-3.in silico     61  CAGCGGCGACAGATGAGCACATCGGTGTTCAATCTCAACTCGAATCCACGTCGACC-ACT 120


11321R-3.IR_full       121 GTCCGAGGCCCAAAACGGAGGTGCCTGGCNTCGCCAATCAGCGCATCCAGCAGGCCCAAT 180
                           ||||||||||||||||||||||||||||| ||||||||||||||||||||||||||||| 
11321R-3.in silico     121 GTCCGAGGCCCAAAACGGAGGTGCCTGGC-TCGCCAATCAGCGCATCCAGCAGGCCCAA- 180


11321R-3.IR_full       181 TCCCTGGCGCAGCTAAACTGCGCTGGATGTCAACAGAGTCAGCAGCATCCTGGCTGGGCA 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11321R-3.in silico     181 TCCCTGGCGCAGCTAAACTGCGCTGGATGTCAACAGAGTCAGCAGCATCCTGGCTGGGCA 240


11321R-3.IR_full        241 CAGCATCCGCACCAAGCACTTCCTCAGCACCAGCATCCCGATCAGTGGTCACAGTTCGG 299
                            |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11321R-3.in silico      241 CAGCATCCGCACCAAGCACTTCCTCAGCACCAGCATCCCGATCAGTGGTCACAGTTCGG 299


11321R-3.IR_full       301 CTCCCAACAGCAATTCAACAACTCCAATCTGTCCCTGAACGTTGGACCGGGTTACATGTC 359
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11321R-3.in silico     301 CTCCCAACAGCAATTCAACAACTCCAATCTGTCCCTGAACGTTGGACCGGGTTACATGTC 359


11321R-3.IR_full       361 GCAGCAACATCATCCACACTATCCGCCACCAGTGTTTATGACCCAGCGTGGTATGATGCC 419
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11321R-3.in silico     361 GCAGCAACATCATCCACACTATCCGCCACCAGTGTTTATGACCCAGCGTGGTATGATGCC 419


11321R-3.IR_full       421 CAATGTGTATCCAGGAGCACCAGGCTATCCCATGATGCATCCAGGTGTCATGGGAATGCC 479
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11321R-3.in silico     421 CAATGTGTATCCAGGAGCACCAGGCTATCCCATGATGCATCCAGGTGTCATGGGAATGCC 479


11321R-3.IR_full       481 ACCTTCAGCTGCCTCCAGAGCTGC 503
                           ||||||||||||||||||||||||
11321R-3.in silico     481 ACCTTCAGCTGCCTCCAGAGCTGC 503

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_135228.1	CG11321-RA, transcript variant A (CG11321), mRNA
97.09	468	2	2	2	NM_164709.1	CG11321-RB, transcript variant B (CG11321), mRNA
0	0	4	3	4	NM_137193.1	CG12959-RA (CG12959), mRNA
0	0	1	4	5	NM_206332.1	CG18331-RA (CG18331), mRNA
0	0	1	4	4	NM_166983.1	CG13316-RA, transcript variant A (Mnt), mRNA
0	0	1	4	4	NM_166984.1	CG13316-RC, transcript variant C (Mnt), mRNA
0	0	1	4	3	NM_137721.1	CG18375-RA, transcript variant A (CG18375), mRNA
0	0	1	4	3	NM_176243.1	CG18375-RB, transcript variant B (CG18375), mRNA
0	0	1	3	9	NM_140328.4	CG10686-RA (tral), mRNA
0	0	1	3	2	NM_136247.2	CG9252-RA (CG9252), mRNA
0	0	1	2	10	NM_001014681.1	CG33553-RD, transcript variant D (Doa), mRNA
0	0	1	2	7	NM_001043298.1	CG33553-RH, transcript variant H (Doa), mRNA
0	0	1	2	5	NM_135766.2	CG16974-RA (CG16974), mRNA
0	0	1	2	3	NM_136737.1	CG12898-RA (CG12898), mRNA
0	0	1	2	3	NM_001032052.1	CG33715-RE, transcript variant E (Msp-300), mRNA
0	0	1	2	3	NM_057938.3	CG3093-RA (dor), mRNA
0	0	1	2	3	NM_080033.2	CG1624-RC, transcript variant C (dpld), mRNA
0	0	1	2	3	NM_165534.1	CG1624-RB, transcript variant B (dpld), mRNA
0	0	1	2	3	NM_165533.1	CG1624-RA, transcript variant A (dpld), mRNA
0	0	1	2	3	NM_143213.2	CG12290-RA (CG12290), mRNA
0	0	1	2	3	NM_143376.1	CG12558-RA (CG12558), mRNA
0	0	1	2	2	NM_140690.2	CG7729-RA (Fit2), mRNA
0	0	1	2	2	NM_079985.2	CG5206-RA (bon), mRNA
0	0	1	2	2	NM_165952.1	CG30488-RA (CG30488), mRNA
0	0	0	6	6	NM_139371.1	CG13917-RA (CG13917), mRNA
0	0	0	5	10	NM_143360.2	CG1345-RA (Gfat2), mRNA
0	0	0	5	7	NM_167164.2	CG12737-RB, transcript variant B (Crag), mRNA
0	0	0	5	7	NM_080341.2	CG12737-RA, transcript variant A (Crag), mRNA
0	0	0	5	3	NM_139541.1	CG12017-RA (CG12017), mRNA
0	0	0	5	2	NM_141014.1	CG10588-RA (CG10588), mRNA

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