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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 11301R-1 
 Symbol Mes4  Full Name Mes4 
 CG No CG11301  Old CG No CG11301 
 Synonyms NF-Yc, CG11301, BcDNA:GM21739, Mes4 
 Accession No (Link to NCBI) NM_137825.1 
 Inserted Chr. ll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgATGGCCAGCGAGGAACTATTT 
 Primer Seq. 3' 
aatctagaggtaccTGGCTCCATCAAGGAATAGCA 
 Predicted Fragment Size 
457 
 IR fragment full Seq 
0001 ATGGCCAGCG AGGAACTATT TGAGGCTGAA TTCTCGGAAG AACAGGACTT GGAGCATCAG 
0061 CAGGCCATGG AGACTGAGGA GGCGGAGCTA GCGGAAACAG AAGAGCCTTT GGAAATCACC 
0121 GAGGAATCGC CGGATAATCC TGAGGCAGAA TCTACAACAG AACAATTGGC AGAAAAACCA 
0181 GTGACGAATG GCAACAAGGC CCCTGCTGAT AACGAAGCCA AGATGACACA ACTGCCGTTG 
0241 GCCAGGATAC GGAACATAAT GAAACTAGAT CCGGATCTGC ATATGGCCAA CAATGAGGCC 
0301 GTGTTCATAG TGGCGAAGGC AGTGGAACTA TTCATAGCCT CGTTGTCACG GGAATCATAT 
0361 ACCTACACGG CGCAGTCCAA AAAGAAAACC ATACAAAAGA GGGACGTGGA TATGGCCATT 
0421 TCTGCTGTGG ACAGTTTGCT ATTCCTTGAT GGAGCCA 
 in silico PCR Fragment 
0001 ATGGCCAGCG AGGAACTATT TGAGGCTGAA TTCTCGGAAG AACAGGACTT GGAGCATCAG 
0061 CAGGCCATGG AGACTGAGGA GGCGGAGCTA GCGGAAACAG AAGAGCCTTT GGAAATCACC 
0121 GAGGAATCGC CGGATAATCC TGAGGCAGAA TCTACAACAG AACAATTGAC AGAAAAACCA 
0181 GTGACGAATG GCAACAAGGC CCCTGCTGAT AACGAAGCCA AGATGACACA ACTGCCGTTG 
0241 GCCAGGATAC GGAACATAAT GAAACTAGAT CCGGATCTGC ATATGGCCAA CAATGAGGCC 
0301 GTGTTCATAG TGGCGAAGGC AGTGGAACTA TTCATAGCCT CGTTGTCACG GGAATCATAT 
0361 ACCTACACGG CGCAGTCCAA AAAGAAAACC ATACAAAAGA GGGACGTGGA TATGGCCATT 
0421 TCTGCTGTGG ACAGTTTGCT ATTCCTTGAT GGAGCCA 
 Assemble Data 

11301R-1.IR_full       1   ATGGCCAGCGAGGAACTATTTGAGGCTGAATTCTCGGAAGAACAGGACTTGGAGCATCAG 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11301R-1.in silico     1   ATGGCCAGCGAGGAACTATTTGAGGCTGAATTCTCGGAAGAACAGGACTTGGAGCATCAG 60


11301R-1.IR_full       61  CAGGCCATGGAGACTGAGGAGGCGGAGCTAGCGGAAACAGAAGAGCCTTTGGAAATCACC 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11301R-1.in silico     61  CAGGCCATGGAGACTGAGGAGGCGGAGCTAGCGGAAACAGAAGAGCCTTTGGAAATCACC 120


11301R-1.IR_full       121 GAGGAATCGCCGGATAATCCTGAGGCAGAATCTACAACAGAACAATTGGCAGAAAAACCA 180
                           |||||||||||||||||||||||||||||||||||||||||||||||| |||||||||||
11301R-1.in silico     121 GAGGAATCGCCGGATAATCCTGAGGCAGAATCTACAACAGAACAATTGACAGAAAAACCA 180


11301R-1.IR_full       181 GTGACGAATGGCAACAAGGCCCCTGCTGATAACGAAGCCAAGATGACACAACTGCCGTTG 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11301R-1.in silico     181 GTGACGAATGGCAACAAGGCCCCTGCTGATAACGAAGCCAAGATGACACAACTGCCGTTG 240


11301R-1.IR_full       241 GCCAGGATACGGAACATAATGAAACTAGATCCGGATCTGCATATGGCCAACAATGAGGCC 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11301R-1.in silico     241 GCCAGGATACGGAACATAATGAAACTAGATCCGGATCTGCATATGGCCAACAATGAGGCC 300


11301R-1.IR_full       301 GTGTTCATAGTGGCGAAGGCAGTGGAACTATTCATAGCCTCGTTGTCACGGGAATCATAT 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11301R-1.in silico     301 GTGTTCATAGTGGCGAAGGCAGTGGAACTATTCATAGCCTCGTTGTCACGGGAATCATAT 360


11301R-1.IR_full       361 ACCTACACGGCGCAGTCCAAAAAGAAAACCATACAAAAGAGGGACGTGGATATGGCCATT 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11301R-1.in silico     361 ACCTACACGGCGCAGTCCAAAAAGAAAACCATACAAAAGAGGGACGTGGATATGGCCATT 420


11301R-1.IR_full       421 TCTGCTGTGGACAGTTTGCTATTCCTTGATGGAGCCA 457
                           |||||||||||||||||||||||||||||||||||||
11301R-1.in silico     421 TCTGCTGTGGACAGTTTGCTATTCCTTGATGGAGCCA 457



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   439  0  0  0  NM_137825.1  CG11301-RA (Mes4), mRNA 
0   0  3  4  5  NM_132122.2  CG3126-RA, transcript variant A (C3G), mRNA 
0   0  3  4  5  NM_176694.1  CG3126-RB, transcript variant B (C3G), mRNA 
0   0  3  2  2  NM_166558.1  CG3510-RC, transcript variant C (CycB), mRNA 
0   0  3  2  2  NM_166557.1  CG3510-RB, transcript variant B (CycB), mRNA 
0   0  3  2  2  NM_166556.1  CG3510-RD, transcript variant D (CycB), mRNA 
0   0  3  2  2  NM_166555.1  CG3510-RA, transcript variant A (CycB), mRNA 
0   0  2  5  2  NM_140833.2  CG10424-RA (CG10424), mRNA 
0   0  1  8  2  NM_079578.2  CG4029-RA (jumu), mRNA 
0   0  1  4  3  NM_168293.1  CG32030-RA, transcript variant A (CG32030), mRNA 
0   0  1  4  2  NM_168294.1  CG32030-RB, transcript variant B (CG32030), mRNA 
0   0  1  3  2  NM_170188.1  CG6422-RB, transcript variant B (CG6422), mRNA 
0   0  1  3  2  NM_143065.2  CG6422-RA, transcript variant A (CG6422), mRNA 
0   0  1  2  3  NM_139936.1  CG13678-RA (CG13678), mRNA 
0   0  1  2  2  NM_134495.2  CG12234-RA (Ranbp21), mRNA 
0   0  0  7  3  NM_137806.2  CG5465-RA (MED16), mRNA 
0   0  0  6  7  NM_137189.2  CG8166-RA (unc-5), mRNA 
0   0  0  5  4  NM_143496.3  CG15523-RA (CG15523), mRNA 
0   0  0  5  2  NM_130505.2  CG13359-RA (CG13359), mRNA 
0   0  0  4  15  NM_139708.1  CG4835-RA (CG4835), mRNA 
0   0  0  4  8  NM_140284.1  CG6793-RA (CG6793), mRNA 
0   0  0  4  6  NM_136257.2  CG8677-RA (CG8677), mRNA 
0   0  0  4  2  NM_079746.4  CG5320-RA, transcript variant A (Gdh), mRNA 
0   0  0  4  2  NM_206551.1  CG5320-RF, transcript variant F (Gdh), mRNA 
0   0  0  4  2  NR_002021.1  CR33328, miscRNA 
0   0  0  3  11  NM_080092.2  CG13207-RB, transcript variant B (nompA), mRNA 
0   0  0  3  11  NM_165821.2  CG13207-RC, transcript variant C (nompA), mRNA 
0   0  0  3  11  NM_165822.2  CG13207-RA, transcript variant A (nompA), mRNA 
0   0  0  3  11  NM_206096.1  CG13207-RD, transcript variant D (nompA), mRNA 
0   0  0  3  10  NM_168802.1  CG32210-RA (CG32210), mRNA 

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