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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 11291R-1 
 Symbol CG11291  Full Name CG11291 
 CG No CG11291  Old CG No CG11291 
 Synonyms CG11291 
 Accession No (Link to NCBI) NM_137812.2 
 Inserted Chr. ll 
 Insertional Mutation  lethal 
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgCAGTCTAAGCCATCTGGACAA 
 Primer Seq. 3' 
aatctagaggtaccCAAGCAACTCGTTCGCTATCA 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 CAGTCTAAGC CATCTGGACA AGCTGCCGAA ggcAAAGGTG GCCGAATGTG ctGGCCGGGA 
0061 TCGACACCAT TATTTCGCAG GCACGGATTG GGGGTGCTGT GGCAGGAGAA TACNNCCCCA 
0121 TTGAGGGGCT CTGTAGAGGC ATTCAATGCC ATAATTTCAA AGGGAAAGCG ATGTCTCATA 
0181 GNCCACCAAC GAGTGCTGCC TNAACAAACA AGGATNCTGT TCCAGAAGGC CAAGTGCCTG 
0241 GGCNTTCAAT GTCAAGNAGC AGGACATTTT CAGCTCGTCG GGTGCCATTG CGAGTTACCT 
0301 TAGCGACCGG AAGTTTAAGA AGAAAATCCT CGTTTTGGGT GGTGACGGTA TCCGCAAAGA 
0361 TCTGAAAGAG GCCGGATTCT GTTCCGTGGT AAACGATCTG CAGCCAAATG ACCAGAAAAA 
0421 AATCGaCTTC GTCAGGAGCT TAGTTCTCGC ATCCGGATGT GGGAGCTNTT TTGGTGGCCA 
0481 GAGACGACAA CATGATAGCG AACGAGTTGC TTN 
 in silico PCR Fragment 
0001 CAGTCTAAGC CATCTGGACA AGCTGCCGAA GGCAAAGGTG GCCGAATG-G CTGGCCGGGA 
0061 TCGACACCAT TATTT-GCA- GCACGGAT-- GGGGTGCTGT GGCAGGAGAA TAC--CCCCA 
0121 TTGA-GGGCT CTGTAGAGGC ATTCAATGCC ATAATTTCAA AGGGAAAGCG ATGTCTCATA 
0181 G-CCACCAAC GAGTGCTGCC T-AACAAACA AGGAT-CTGT TCCAGAAGGC CAAGTGCCTG 
0241 GGC-TTCAAT GTCAAGGAGC AGGACATTTT CAGCTCGTCG GGTGCCATTG CGAGTTACCT 
0301 TAGCGACCGG AAGTTTAAGA AGAAAATCCT CGTTTTGGGT GGTGACGGTA TCCGCAAAGA 
0361 TCTGAAAGAG GCCGGATTCT GTTCCGTGGT AAACGATCTG CAGCCAAATG ACCAGAAAAA 
0421 AATCGACTTC GTCAGGAGCT TAGTTCTCG- ATCCGGATGT GGGAGCTGTT TTGGTGGCCA 
0481 GAGACGACAA CATGATAGCG AACGAGTTGC TTG 
 Assemble Data 

11291R-1.IR_full       1   CAGTCTAAGCCATCTGGACAAGCTGCCGAAGGCAAAGGTGGCCGAATGTGCTGGCCGGGA 60
                           |||||||||||||||||||||||||||||||||||||||||||||||| |||||||||||
11291R-1.in silico     1   CAGTCTAAGCCATCTGGACAAGCTGCCGAAGGCAAAGGTGGCCGAATG-GCTGGCCGGGA 60


11291R-1.IR_full       61  TCGACACCATTATTTCGCAGGCACGGATTGGGGGTGCTGTGGCAGGAGAATACNNCCCCA 120
                           ||||||||||||||| ||| ||||||||  |||||||||||||||||||||||  |||||
11291R-1.in silico     61  TCGACACCATTATTT-GCA-GCACGGAT--GGGGTGCTGTGGCAGGAGAATAC--CCCCA 120


11291R-1.IR_full       121 TTGAGGGGCTCTGTAGAGGCATTCAATGCCATAATTTCAAAGGGAAAGCGATGTCTCATA 180
                           |||| |||||||||||||||||||||||||||||||||||||||||||||||||||||||
11291R-1.in silico     121 TTGA-GGGCTCTGTAGAGGCATTCAATGCCATAATTTCAAAGGGAAAGCGATGTCTCATA 180


11291R-1.IR_full       181 GNCCACCAACGAGTGCTGCCTNAACAAACAAGGATNCTGTTCCAGAAGGCCAAGTGCCTG 240
                           | ||||||||||||||||||| ||||||||||||| ||||||||||||||||||||||||
11291R-1.in silico     181 G-CCACCAACGAGTGCTGCCT-AACAAACAAGGAT-CTGTTCCAGAAGGCCAAGTGCCTG 240


11291R-1.IR_full       241 GGCNTTCAATGTCAAGNAGCAGGACATTTTCAGCTCGTCGGGTGCCATTGCGAGTTACCT 300
                           ||| |||||||||||| |||||||||||||||||||||||||||||||||||||||||||
11291R-1.in silico     241 GGC-TTCAATGTCAAGGAGCAGGACATTTTCAGCTCGTCGGGTGCCATTGCGAGTTACCT 300


11291R-1.IR_full       301 TAGCGACCGGAAGTTTAAGAAGAAAATCCTCGTTTTGGGTGGTGACGGTATCCGCAAAGA 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11291R-1.in silico     301 TAGCGACCGGAAGTTTAAGAAGAAAATCCTCGTTTTGGGTGGTGACGGTATCCGCAAAGA 360


11291R-1.IR_full       361 TCTGAAAGAGGCCGGATTCTGTTCCGTGGTAAACGATCTGCAGCCAAATGACCAGAAAAA 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11291R-1.in silico     361 TCTGAAAGAGGCCGGATTCTGTTCCGTGGTAAACGATCTGCAGCCAAATGACCAGAAAAA 420


11291R-1.IR_full       421 AATCGACTTCGTCAGGAGCTTAGTTCTCGCATCCGGATGTGGGAGCTNTTTTGGTGGCCA 480
                           ||||||||||||||||||||||||||||| ||||||||||||||||| ||||||||||||
11291R-1.in silico     421 AATCGACTTCGTCAGGAGCTTAGTTCTCG-ATCCGGATGTGGGAGCTGTTTTGGTGGCCA 480


11291R-1.IR_full       481 GAGACGACAACATGATAGCGAACGAGTTGCTTN 513
                           |||||||||||||||||||||||||||||||| 
11291R-1.in silico     481 GAGACGACAACATGATAGCGAACGAGTTGCTTG 513



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_137812.2  CG11291-RA (CG11291), mRNA 
0   0  2  4  2  NM_176520.1  CG33094-RB (Synd), mRNA 
0   0  2  3  4  NM_206506.1  CG7467-RC, transcript variant C (osa), mRNA 
0   0  2  3  4  NM_079668.2  CG7467-RB, transcript variant B (osa), mRNA 
0   0  2  3  4  NM_169775.1  CG7467-RA, transcript variant A (osa), mRNA 
0   0  2  3  3  NM_134720.2  CG4629-RA, transcript variant A (CG4629), mRNA 
0   0  2  3  3  NM_164404.2  CG4629-RB, transcript variant B (CG4629), mRNA 
0   0  2  3  3  NM_205888.1  CG4629-RC, transcript variant C (CG4629), mRNA 
0   0  1  3  5  NM_141300.1  CG2919-RA (CG2919), mRNA 
0   0  1  3  3  NM_080074.2  CG10197-RA (kn), mRNA 
0   0  1  3  2  NM_132524.1  CG1895-RA (Cyp28c1), mRNA 
0   0  1  2  5  NM_166546.2  CG30092-RD, transcript variant D (jbug), mRNA 
0   0  1  2  2  NM_164369.1  CG31975-RA (CG31975), mRNA 
0   0  1  2  2  NM_139910.2  CG8209-RA (CG8209), mRNA 
0   0  0  8  3  NM_133154.2  CG12199-RA, transcript variant A (kek5), mRNA 
0   0  0  8  3  NM_167647.2  CG12199-RB, transcript variant B (kek5), mRNA 
0   0  0  7  2  NM_167346.1  CG4353-RA, transcript variant A (hep), mRNA 
0   0  0  7  2  NM_136425.2  CG11107-RA (CG11107), mRNA 
0   0  0  7  2  NM_078587.2  CG4353-RC, transcript variant C (hep), mRNA 
0   0  0  6  3  NM_206308.2  CG4432-RC, transcript variant C (PGRP-LC), mRNA 
0   0  0  6  2  NM_141538.1  CG7352-RA (CG7352), mRNA 
0   0  0  5  6  NM_169125.1  CG31557-RA (Obp83ef), mRNA 
0   0  0  5  5  NM_169878.1  CG4572-RA, transcript variant A (CG4572), mRNA 
0   0  0  5  5  NM_142579.2  CG4572-RB, transcript variant B (CG4572), mRNA 
0   0  0  5  5  NM_169879.1  CG4572-RC, transcript variant C (CG4572), mRNA 
0   0  0  5  4  NM_136733.2  CG12907-RA (CG12907), mRNA 
0   0  0  5  3  NM_136365.1  CG11212-RA (Ptr), mRNA 
0   0  0  5  2  NM_133132.1  CG14194-RA (CG14194), mRNA 
0   0  0  4  11  NM_001032051.1  CG33715-RD, transcript variant D (Msp-300), mRNA 
0   0  0  4  8  NM_001032053.1  CG33715-RB, transcript variant B (Msp-300), mRNA 

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