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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
11290R-1 |
| Symbol |
enok |
Full Name |
enoki mushroom |
| CG No |
CG11290 |
Old CG No |
CG11290 |
| Synonyms |
dmHAG406, anon-WO0200864.2, CG11290, rot, S1, unnamed, anon-WO0200864.1, anon-60Ba, enok |
| Accession No (Link to NCBI) |
NM_079114.1 |
| Inserted Chr. |
ll |
| Insertional Mutation |
semi-lethal |
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Cho Y, Lai CM, Lin KY, Hsu HJ.
A Targeted RNAi Screen Reveals Drosophila Female-Sterile Genes That Control the Size of Germline Stem Cell Niche During Development.
G3 (Bethesda) (2018) 8(7) 2345-2354
[
PubMed ID = 29764959
]
[
RRC reference
]
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Molnar C, Casado M, López-Varea A, Cruz C, de Celis JF.
Genetic annotation of gain-of-function screens using RNA interference and in situ hybridization of candidate genes in the Drosophila wing.
Genetics (2012) 192(2) 741-52
[
PubMed ID = 22798488
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgAAGATCAGGTCCCAGAAGCAG |
| Primer Seq. 3' |
aatctagaggtaccGAATTTCCCGAGCAGTGGGTG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 AAGATCAGGT CCCAGAAGCA GAGGCCCTCC GTGCAGAGGA TTTGCCAGGC GATCGGCACA
0061 CACCACAAGT TCCACGAGGA CATTGTGGCA GAGAAGCTGG AAAAAGCTGT CGAGTCGGGA
0121 GCCGTCATAA AGGTCTACAA CAAGGGACTG CACTCCTACA AGGCACCAAT GGCCAAAGCG
0181 TCGCGTCAAG GTGGACAAGA ACACTAATCT GTACAAATTG GTGNGCCAAG GCGGTCCACG
0241 ACCTGGGCGA ATGCGAGGGA TCTACCATCA AGAGCATTGA GAACTACATT CAGAAGTTCA
0301 ACTGTATCGA CTTGTCGCCC AATGTGGACT TCAAGGCGGT CATTAAGGCT TCGATCAAGA
0361 AGGCCGTGGA TGCCGGCTTT CTGATCCAGG AGGGGAAGCT ATATAAAAAG GGCAAATCCT
0421 TGACAACCCC ACGGAAATGC GCACCCGTCT CGGAGGTGGT CATAAAGGGC GAGGAGTCCT
0481 GCACCCACTG CTCGGGAAAT TC |
| in silico PCR Fragment |
0001 AAGATCAGGT CCCAGAAGCA GAGGCCCTCC GTGCAGAGGA TTTGCCAGGC GATCGGCACA
0061 CACCACAAGT TCCACGAGGA CATTGTGGCA GAGAAGCTGG AAAAAGCTGT CGAGTCGGGA
0121 GCCGTCATAA AGGTCTACAA CAAGGGACTG CACTCCTACA AGGCACCAAT GGCC-AAGCG
0181 TCGCGTCAAG GTGGACAAGA ACACTAATCT GTACAAATTG GTG-GCCAAG GCGGTCCACG
0241 ACCTGGGCGA ATGCGAGGGA TCTACCATCA AGAGCATTGA GAACTACATT CAGAAGTTCA
0301 ACTGTATCGA CTTGTCGCCC AATGTGGACT TCAAGGCGGT CATTAAGGCT TCGATCAAGA
0361 AGGCCGTGGA TGCCGGCTTT CTGATCCAGG AGGGGAAGCT ATATAAAAAG GGCAAATCCT
0421 TGACAACCCC ACGGAAATGC GCACCCGTCT CGGAGGTGGT CATAAAGGGC GAGGAGTCCT
0481 GCACCCACTG CTCGGGAAAT TC |
| Assemble Data |
11290R-1.IR_full 1 AAGATCAGGTCCCAGAAGCAGAGGCCCTCCGTGCAGAGGATTTGCCAGGCGATCGGCACA 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11290R-1.in silico 1 AAGATCAGGTCCCAGAAGCAGAGGCCCTCCGTGCAGAGGATTTGCCAGGCGATCGGCACA 60
11290R-1.IR_full 61 CACCACAAGTTCCACGAGGACATTGTGGCAGAGAAGCTGGAAAAAGCTGTCGAGTCGGGA 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11290R-1.in silico 61 CACCACAAGTTCCACGAGGACATTGTGGCAGAGAAGCTGGAAAAAGCTGTCGAGTCGGGA 120
11290R-1.IR_full 121 GCCGTCATAAAGGTCTACAACAAGGGACTGCACTCCTACAAGGCACCAATGGCCAAAGCG 180
|||||||||||||||||||||||||||||||||||||||||||||||||||||| |||||
11290R-1.in silico 121 GCCGTCATAAAGGTCTACAACAAGGGACTGCACTCCTACAAGGCACCAATGGCC-AAGCG 180
11290R-1.IR_full 181 TCGCGTCAAGGTGGACAAGAACACTAATCTGTACAAATTGGTGNGCCAAGGCGGTCCACG 240
||||||||||||||||||||||||||||||||||||||||||| ||||||||||||||||
11290R-1.in silico 181 TCGCGTCAAGGTGGACAAGAACACTAATCTGTACAAATTGGTG-GCCAAGGCGGTCCACG 240
11290R-1.IR_full 241 ACCTGGGCGAATGCGAGGGATCTACCATCAAGAGCATTGAGAACTACATTCAGAAGTTCA 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11290R-1.in silico 241 ACCTGGGCGAATGCGAGGGATCTACCATCAAGAGCATTGAGAACTACATTCAGAAGTTCA 300
11290R-1.IR_full 301 ACTGTATCGACTTGTCGCCCAATGTGGACTTCAAGGCGGTCATTAAGGCTTCGATCAAGA 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11290R-1.in silico 301 ACTGTATCGACTTGTCGCCCAATGTGGACTTCAAGGCGGTCATTAAGGCTTCGATCAAGA 360
11290R-1.IR_full 361 AGGCCGTGGATGCCGGCTTTCTGATCCAGGAGGGGAAGCTATATAAAAAGGGCAAATCCT 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11290R-1.in silico 361 AGGCCGTGGATGCCGGCTTTCTGATCCAGGAGGGGAAGCTATATAAAAAGGGCAAATCCT 420
11290R-1.IR_full 421 TGACAACCCCACGGAAATGCGCACCCGTCTCGGAGGTGGTCATAAAGGGCGAGGAGTCCT 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11290R-1.in silico 421 TGACAACCCCACGGAAATGCGCACCCGTCTCGGAGGTGGTCATAAAGGGCGAGGAGTCCT 480
11290R-1.IR_full 481 GCACCCACTGCTCGGGAAATTC 502
||||||||||||||||||||||
11290R-1.in silico 481 GCACCCACTGCTCGGGAAATTC 502
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_079114.1 |
CG11290-RA (enok), mRNA |
| 0.2 |
1 |
2 |
2 |
6 |
NM_140521.1 |
CG6498-RA (CG6498), mRNA |
| 0 |
0 |
3 |
3 |
2 |
NM_080091.2 |
CG14993-RA (Faa), mRNA |
| 0 |
0 |
3 |
2 |
4 |
NM_132148.1 |
CG3032-RA (CG3032), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_079571.1 |
CG8374-RA (dmt), mRNA |
| 0 |
0 |
1 |
4 |
7 |
NR_001305.1 |
CG8374-RA (dmt), mRNA, mRNA |
| 0 |
0 |
1 |
4 |
4 |
NM_080317.2 |
CG2647-RA (per), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_164399.1 |
CG4226-RB, transcript variant B (GluRIIC), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_134713.2 |
CG4226-RA, transcript variant A (GluRIIC), mRNA |
| 0 |
0 |
1 |
3 |
16 |
NM_141666.2 |
CG9492-RA (CG9492), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_136257.2 |
CG8677-RA (CG8677), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_136155.2 |
CG10628-RA (CG10628), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_166099.1 |
CG8182-RB, transcript variant B (GalNAc-T1), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_137199.2 |
CG8182-RA, transcript variant A (GalNAc-T1), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_169061.1 |
CG8182-RA, transcript variant A (GalNAc-T1), mRNA,4,5,-tris-phosphate receptor CG1063-RA, transcript variant A (Itp-r83A), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_169060.1 |
CG8182-RA, transcript variant A (GalNAc-T1), mRNA,4,5,-tris-phosphate receptor CG1063-RA, transcript variant A (Itp-r83A), mRNA,4,5,-tris-phosphate receptor CG1063-RB, transcript variant B (Itp-r83A), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_136851.1 |
CG13196-RA (CG13196), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_134690.2 |
CG2807-RA (CG2807), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_133022.1 |
CG6762-RA (CG6762), mRNA |
| 0 |
0 |
0 |
6 |
8 |
NM_057678.3 |
CG31240-RA (repo), mRNA |
| 0 |
0 |
0 |
6 |
3 |
NM_138193.1 |
CG17180-RA (CG17180), mRNA |
| 0 |
0 |
0 |
5 |
9 |
NM_135762.2 |
CG16975-RB, transcript variant B (CG16975), mRNA |
| 0 |
0 |
0 |
5 |
3 |
NM_135840.2 |
CG7968-RA (CG7968), mRNA |
| 0 |
0 |
0 |
4 |
7 |
NM_141313.1 |
CG2182-RA, transcript variant A (CG2182), mRNA |
| 0 |
0 |
0 |
4 |
6 |
NM_140067.1 |
CG3654-RD (CG3654), mRNA |
| 0 |
0 |
0 |
4 |
6 |
NM_165184.1 |
CG17927-RJ, transcript variant J (Mhc), mRNA |
| 0 |
0 |
0 |
4 |
6 |
NM_165183.1 |
CG17927-RE, transcript variant E (Mhc), mRNA |
| 0 |
0 |
0 |
4 |
6 |
NM_165182.1 |
CG17927-RG, transcript variant G (Mhc), mRNA |
| 0 |
0 |
0 |
4 |
6 |
NM_165181.1 |
CG17927-RC, transcript variant C (Mhc), mRNA |
| 0 |
0 |
0 |
4 |
6 |
NM_170089.1 |
CG10367-RA, transcript variant A (Hmgcr), mRNA |
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