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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 11262R-2 
 Symbol CG11262  Full Name CG11262 
 CG No CG11262  Old CG No CG11262 
 Synonyms CG11262 
 Accession No (Link to NCBI) NM_140360.1 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgCCTGAACAACCTGAACCGAAC 
 Primer Seq. 3' 
aatctagaggtaccACCGATACCACCATCAGCAGC 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 CCTGAACAAC CTGAACCGAA CTACTTGGAT GAGCCGGAGA CAATCTCCGT TGGGCGATTC 
0061 ATCTTCAAGA TCATAAGGAT TGGTGCGCTT CTGCTGATTT GGGCTTTCTT TACTTACATT 
0121 CTGGTTCGGA ATGCCACCAC GCCGGATTTG ACATCGGTGG TCACTGTGTT GCCCAACGAG 
0181 ACTGTGCTCC GCCGTCTGTC CGTGCCCCAT GATGCGACTC AGATAACGCT GAAGGGTCCC 
0241 ATCGATTTGC AATTGCAGAA GCATGAAAGC GTGATCATGG AGACGCCCCA TGTGGNCGTA 
0301 CNGCGTGGAG TGGCGGGATT CGGAATTGAA AACCAAATTC TGGAGTTCGG ATATGTGGAT 
0361 TGTCTATCTG GCGGAGCAGA GTCAGGCTTT CACGAAGACG TCCAAAACCG TTCAGATCTC 
0421 GGAGGTTAGT AATGCCCAGG CGGTGATTTC GCTGGAGAGC AGAAGCGAGG AgCCGGTgGC 
0481 GCTGCTGATG GTGGTATCGG T 
 in silico PCR Fragment 
0001 CCTGAACAAC CTGAACCGAA CTACTTGGAT GAGCCGGAGA CAATCTCCGT CGGGCGATTC 
0061 ATCTTCAAGA TCATAAGGAT TGGTGCGCTT CTGCTGATTT GGGCTTTCTT TACTTACATT 
0121 CTGGTTCGGA ATGCCACCAC GCCGGATTTG ACATCGGTGG TCACTGTGTT GCCCAACGAG 
0181 ACTGTGCTCC GCCGCCTGTC CGTGCCCCAT GATGCGACTC AGATAACGCT GAAGGGTCCC 
0241 ATCGATTTGC AATTGCAGAA GCATGAAAGC GTGATTATGG AGACGCCCCA TGTGGGCGTA 
0301 C-GCGTGGAG TGGCGGGATT CGGAATTGAA AACCAAATTC TGGAGTTCGG ATATGTGGAT 
0361 TGTCTATCTG GCCGAGCAGA GTCAGGCTTT CACGAAGACG TCCAAAACCG TTCAGATCTC 
0421 GGAGGTTAGT AATGCCCAGG CGGTGATTTC GCTGGAGAGC AGAAGCGAGG AGCCGGTGGC 
0481 GCTGCTGATG GTGGTATCGG T 
 Assemble Data 

11262R-2.IR_full       1   CCTGAACAACCTGAACCGAACTACTTGGATGAGCCGGAGACAATCTCCGTTGGGCGATTC 60
                           |||||||||||||||||||||||||||||||||||||||||||||||||| |||||||||
11262R-2.in silico     1   CCTGAACAACCTGAACCGAACTACTTGGATGAGCCGGAGACAATCTCCGTCGGGCGATTC 60


11262R-2.IR_full       61  ATCTTCAAGATCATAAGGATTGGTGCGCTTCTGCTGATTTGGGCTTTCTTTACTTACATT 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11262R-2.in silico     61  ATCTTCAAGATCATAAGGATTGGTGCGCTTCTGCTGATTTGGGCTTTCTTTACTTACATT 120


11262R-2.IR_full       121 CTGGTTCGGAATGCCACCACGCCGGATTTGACATCGGTGGTCACTGTGTTGCCCAACGAG 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11262R-2.in silico     121 CTGGTTCGGAATGCCACCACGCCGGATTTGACATCGGTGGTCACTGTGTTGCCCAACGAG 180


11262R-2.IR_full       181 ACTGTGCTCCGCCGTCTGTCCGTGCCCCATGATGCGACTCAGATAACGCTGAAGGGTCCC 240
                           |||||||||||||| |||||||||||||||||||||||||||||||||||||||||||||
11262R-2.in silico     181 ACTGTGCTCCGCCGCCTGTCCGTGCCCCATGATGCGACTCAGATAACGCTGAAGGGTCCC 240


11262R-2.IR_full       241 ATCGATTTGCAATTGCAGAAGCATGAAAGCGTGATCATGGAGACGCCCCATGTGGNCGTA 300
                           ||||||||||||||||||||||||||||||||||| ||||||||||||||||||| ||||
11262R-2.in silico     241 ATCGATTTGCAATTGCAGAAGCATGAAAGCGTGATTATGGAGACGCCCCATGTGGGCGTA 300


11262R-2.IR_full       301 CNGCGTGGAGTGGCGGGATTCGGAATTGAAAACCAAATTCTGGAGTTCGGATATGTGGAT 360
                           | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11262R-2.in silico     301 C-GCGTGGAGTGGCGGGATTCGGAATTGAAAACCAAATTCTGGAGTTCGGATATGTGGAT 360


11262R-2.IR_full       361 TGTCTATCTGGCGGAGCAGAGTCAGGCTTTCACGAAGACGTCCAAAACCGTTCAGATCTC 420
                           |||||||||||| |||||||||||||||||||||||||||||||||||||||||||||||
11262R-2.in silico     361 TGTCTATCTGGCCGAGCAGAGTCAGGCTTTCACGAAGACGTCCAAAACCGTTCAGATCTC 420


11262R-2.IR_full       421 GGAGGTTAGTAATGCCCAGGCGGTGATTTCGCTGGAGAGCAGAAGCGAGGAGCCGGTGGC 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11262R-2.in silico     421 GGAGGTTAGTAATGCCCAGGCGGTGATTTCGCTGGAGAGCAGAAGCGAGGAGCCGGTGGC 480


11262R-2.IR_full       481 GCTGCTGATGGTGGTATCGGT 501
                           |||||||||||||||||||||
11262R-2.in silico     481 GCTGCTGATGGTGGTATCGGT 501



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_140360.1  CG11262-RA (CG11262), mRNA 
0.2   1  3  2  2  NM_136553.2  CG8639-RA (Cirl), mRNA 
0   0  3  2  7  NM_135847.4  CG31765-RA (CG31765), mRNA 
0   0  2  3  3  NM_205952.1  CG4128-RD, transcript variant D (nAcRalpha-30D), mRNA 
0   0  2  3  3  NM_205953.1  CG4128-RE, transcript variant E (nAcRalpha-30D), mRNA 
0   0  2  3  3  NM_164874.2  CG4128-RA, transcript variant A (nAcRalpha-30D), mRNA 
0   0  2  3  3  NM_205951.1  CG4128-RB, transcript variant B (nAcRalpha-30D), mRNA 
0   0  2  3  3  NM_135472.4  CG4128-RC, transcript variant C (nAcRalpha-30D), mRNA 
0   0  1  5  4  NM_137336.2  CG8963-RA, transcript variant A (CG8963), mRNA 
0   0  1  5  4  NM_206145.1  CG8963-RB, transcript variant B (CG8963), mRNA 
0   0  1  5  3  NM_142370.1  CG5873-RA (CG5873), mRNA 
0   0  1  4  14  NM_132114.1  CG14442-RA (CG14442), mRNA 
0   0  1  4  5  NM_001038959.1  CG34055-RA (CG34055), mRNA 
0   0  1  4  4  NM_143057.2  CG11771-RA (CG11771), mRNA 
0   0  1  4  2  NM_164996.1  CG31760-RA (CG31760), mRNA 
0   0  1  3  10  NM_164971.1  CG14938-RD, transcript variant D (crol), mRNA 
0   0  1  3  10  NM_057895.3  CG14938-RA, transcript variant A (crol), mRNA 
0   0  1  3  9  NM_057896.3  CG14938-RC, transcript variant C (crol), mRNA 
0   0  1  3  8  NM_057669.2  CG12399-RA (Mad), mRNA 
0   0  1  3  3  NM_166687.1  CG16932-RB, transcript variant B (Eps-15), mRNA 
0   0  1  3  3  NM_166686.1  CG16932-RC, transcript variant C (Eps-15), mRNA 
0   0  1  3  3  NM_138121.2  CG16932-RA, transcript variant A (Eps-15), mRNA 
0   0  1  3  3  NM_078608.3  CG9176-RC, transcript variant C (cngl), mRNA 
0   0  1  3  3  NM_167441.2  CG9176-RB, transcript variant B (cngl), mRNA 
0   0  1  3  2  NM_136789.2  CG7722-RA (CG7722), mRNA 
0   0  1  2  7  NM_057897.3  CG14938-RB, transcript variant B (crol), mRNA 
0   0  1  2  4  NM_206632.1  CG3566-RC, transcript variant C (CG3566), mRNA 
0   0  1  2  3  NM_144187.1  CG13306-RA (CG13306), mRNA 
0   0  1  2  2  NM_139412.1  CG13936-RA (CG13936), mRNA 
0   0  0  6  8  NM_130685.1  CG14420-RA (CG14420), mRNA 

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