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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 11257R-2 
 Symbol CG11257  Full Name CG11257 
 CG No CG11257  Old CG No CG11257 
 Synonyms CG11257 
 Accession No (Link to NCBI) NM_137575.1 
 Inserted Chr. lll 
 Insertional Mutation  lethal 
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgGATCGGCCACTGGTAATCCGC 
 Primer Seq. 3' 
aatctagaggtaccGACAACCAGGCCTGGATTAGC 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 GATCGGCCAC TGGTAATCCG CGCAACAAGT GCgNCCTAAA GCCGGGCTAC TCCCTGATGA 
0061 ACTGGATACG GCTGTGCAAT TCTGGAGCGG ATCTATCGGG AACCGGAGGG CGTCTGGTGC 
0121 CCGTNGTCGC GAACGGAATT GGCCCGGCAC AACAAGATCG ACGACGCCTG GATGGCCATT 
0181 CGTGGTCGTG TCTTCAATGT CACCCGCTAC ATGGACTTTC ATCCGGGCGG AGTGGATGAG 
0241 CTAATGCGTG GTGTTGGTCG CGATGCCACC AAATTGTTCG ATGAGGTGCA CGCGTGGGTG 
0301 AACTATCCAC AATTGTTGGG AAAGTGCTAC GTGGGACCAC TAAAAGATAA TGAGACAAAA 
0361 CCTGCCAAGG AAAGTCCACA GATTACCAAT GTGATATTGC AGCCTCCGGA AATAGTGCCA 
0421 CGCTTTGATT GGATTCAGCA GAGGAGCGAA CTTACCCTGA TCTTCTACAC GCGCAgNCTG 
0481 GCTAATCCAG GCCTGGTTGT C 
 in silico PCR Fragment 
0001 GATCGGCCAC TGGTAATCCG CGCAACAAGT GCGCCCTAAA GCCGGGCTAC TCCCTGATGA 
0061 ACTGGATACG GCTGTGCAAT TCTGGAGCGG ATCTATCGGG AACCGGAGGG CGTCTGGTGC 
0121 CCGT-GTCGC GAACGGAATT GGCCCGGCAC AACAAGATCG ACGACGCCTG GATGGCCATT 
0181 CGTGGTCGTG TCTTCAATGT CACCCGCTAC ATGGACTTTC ATCCGGGCGG AGTGGATGAG 
0241 CTAATGCGTG GTGTTGGTCG CGATGCCACC AAATTGTTCG ATGAGGTGCA CGCGTGGGTG 
0301 AACTATCCAC AATTGTTGGG AAAGTGCTAC GTGGGACCAC TAAAAGATAA TGAGACAAAA 
0361 CCTGCCAAGG AAAGTCCACA GATTACCAAT GTGATATTGC AGCCTCCGGA AATAGTGCCA 
0421 CGCTTTGATT GGATTCAGCA GAGGAGCGAA CTTACCCTGA TCTTCTACAC GCGCAGCCTG 
0481 GCTAATCCAG GCCTGGTTGT C 
 Assemble Data 

11257R-2.IR_full       1   GATCGGCCACTGGTAATCCGCGCAACAAGTGCGNCCTAAAGCCGGGCTACTCCCTGATGA 60
                           ||||||||||||||||||||||||||||||||| ||||||||||||||||||||||||||
11257R-2.in silico     1   GATCGGCCACTGGTAATCCGCGCAACAAGTGCGCCCTAAAGCCGGGCTACTCCCTGATGA 60


11257R-2.IR_full       61  ACTGGATACGGCTGTGCAATTCTGGAGCGGATCTATCGGGAACCGGAGGGCGTCTGGTGC 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11257R-2.in silico     61  ACTGGATACGGCTGTGCAATTCTGGAGCGGATCTATCGGGAACCGGAGGGCGTCTGGTGC 120


11257R-2.IR_full       121 CCGTNGTCGCGAACGGAATTGGCCCGGCACAACAAGATCGACGACGCCTGGATGGCCATT 180
                           |||| |||||||||||||||||||||||||||||||||||||||||||||||||||||||
11257R-2.in silico     121 CCGT-GTCGCGAACGGAATTGGCCCGGCACAACAAGATCGACGACGCCTGGATGGCCATT 180


11257R-2.IR_full       181 CGTGGTCGTGTCTTCAATGTCACCCGCTACATGGACTTTCATCCGGGCGGAGTGGATGAG 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11257R-2.in silico     181 CGTGGTCGTGTCTTCAATGTCACCCGCTACATGGACTTTCATCCGGGCGGAGTGGATGAG 240


11257R-2.IR_full       241 CTAATGCGTGGTGTTGGTCGCGATGCCACCAAATTGTTCGATGAGGTGCACGCGTGGGTG 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11257R-2.in silico     241 CTAATGCGTGGTGTTGGTCGCGATGCCACCAAATTGTTCGATGAGGTGCACGCGTGGGTG 300


11257R-2.IR_full       301 AACTATCCACAATTGTTGGGAAAGTGCTACGTGGGACCACTAAAAGATAATGAGACAAAA 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11257R-2.in silico     301 AACTATCCACAATTGTTGGGAAAGTGCTACGTGGGACCACTAAAAGATAATGAGACAAAA 360


11257R-2.IR_full       361 CCTGCCAAGGAAAGTCCACAGATTACCAATGTGATATTGCAGCCTCCGGAAATAGTGCCA 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11257R-2.in silico     361 CCTGCCAAGGAAAGTCCACAGATTACCAATGTGATATTGCAGCCTCCGGAAATAGTGCCA 420


11257R-2.IR_full       421 CGCTTTGATTGGATTCAGCAGAGGAGCGAACTTACCCTGATCTTCTACACGCGCAGNCTG 480
                           |||||||||||||||||||||||||||||||||||||||||||||||||||||||| |||
11257R-2.in silico     421 CGCTTTGATTGGATTCAGCAGAGGAGCGAACTTACCCTGATCTTCTACACGCGCAGCCTG 480


11257R-2.IR_full       481 GCTAATCCAGGCCTGGTTGTC 501
                           |||||||||||||||||||||
11257R-2.in silico     481 GCTAATCCAGGCCTGGTTGTC 501



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_137575.1  CG11257-RA (CG11257), mRNA 
0   0  3  2  5  NM_141306.2  CG12171-RA (CG12171), mRNA 
0   0  3  2  5  NM_142779.1  CG13856-RA (CG13856), mRNA 
0   0  2  5  2  NM_167969.1  CG32488-RA (CG32488), mRNA 
0   0  1  7  2  NM_138984.2  CG5712-RA (ACXD), mRNA 
0   0  1  3  4  NM_140881.2  CG8786-RB (CG8786), mRNA 
0   0  1  3  2  NM_168441.1  CG32071-RA (CG32071), mRNA 
0   0  1  2  4  NM_143714.2  CG4746-RA (mab-2), mRNA 
0   0  1  2  2  NM_144380.1  CG3410-RA (lectin-24A), mRNA 
0   0  1  2  2  NM_001015231.1  CG40154-PB.3 (CG40154), mRNA 
0   0  1  2  2  NM_170396.1  CG1709-RD, transcript variant D (Vha100-1), mRNA 
0   0  1  2  2  NM_170392.1  CG1709-RA, transcript variant A (Vha100-1), mRNA 
0   0  1  2  2  NM_143415.2  CG1709-RE, transcript variant E (Vha100-1), mRNA 
0   0  1  2  2  NM_140400.1  CG10083-RA (CG10083), mRNA 
0   0  0  8  5  NM_135958.3  CG5996-RA, transcript variant A (trpgamma), mRNA 
0   0  0  8  5  NM_165169.3  CG5996-RB, transcript variant B (trpgamma), mRNA 
0   0  0  5  9  NM_135760.1  CG5204-RA (CG5204), mRNA 
0   0  0  5  4  NM_164984.1  CG31865-RA (CG31865), mRNA 
0   0  0  5  4  NM_164980.1  CG31866-RA (CG31866), mRNA 
0   0  0  5  3  NM_143018.2  CG5794-RD, transcript variant D (CG5794), mRNA 
0   0  0  5  3  NM_170158.1  CG5794-RB, transcript variant B (CG5794), mRNA 
0   0  0  5  3  NM_135581.1  CG7329-RA (CG7329), mRNA 
0   0  0  4  7  NM_206067.1  CG1429-RF, transcript variant F (Mef2), mRNA 
0   0  0  4  7  NM_057670.2  CG1429-RC, transcript variant C (Mef2), mRNA 
0   0  0  4  7  NM_057672.2  CG1429-RD, transcript variant D (Mef2), mRNA 
0   0  0  4  7  NM_057673.2  CG1429-RA, transcript variant A (Mef2), mRNA 
0   0  0  4  7  NM_165722.1  CG1429-RE, transcript variant E (Mef2), mRNA 
0   0  0  4  7  NM_057671.2  CG1429-RB, transcript variant B (Mef2), mRNA 
0   0  0  4  3  NM_164546.1  CG3851-RA (odd), mRNA 
0   0  0  4  3  NM_206203.1  CG30194-RC, transcript variant C (CG30194), mRNA 

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