NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 11208R-1

Symbol CG11208 Full Name CG11208

CG No CG11208 Old CG No CG11208

Synonyms anon-WO0118547.196, CG11208

Accession No (Link to NCBI) NM_137616.2

Inserted Chr. lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees viable

Map Viewer

Reference
[Please submit your publication] Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340 [ PubMed ID = 25473846 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgCAGGAGGCCAGCATCTACAAG

Primer Seq. 3'

aatctagaggtaccTCGTAGCCGAAGCGGAAGTTA

Predicted Fragment Size

IR fragment full Seq

0001 CAGGAGGCCA GCATCTACAA GGCGCTGGcC CATCCGGCTC CCCCACTAGC CTATCCCCCG 
0061 CACGATGATG TGATCAGGGC CGCCAAGTTG TTGCGACAGG CCAAGCGACC CTTGGTGATC 
0121 GTGGGCAAGG GTTCCGCCTA CGCCCATGCC GAGAACACAC TGCGCCACTT CATCGAGAAC 
0181 ACCAACCTGC CCTTCCTCCC CACCCCCATG GACAAGGGCG TTGTATCCGA TACGGCTCTG 
0241 CAGTGCGTCT CCTCGGCTCG CACCCTGGCC TTGCAAAAGG CCGATGTGGT GCTGCTCCTG 
0301 GGCGCTCGCC TCAACTGGAT TCTCCATTTC GGAAAGGTTC CGCGCTACGA CAAGGACGTG 
0361 AAGTTTATCC AGGTGGACAT CAATCCTGAG GAGCTGCATA ACTCGGTTGT GGCTTCGGTG 
0421 GCCATCCAGG CGGACATCCG TCCATTCGCC GAGCAATTGT TTGAGCAAAT GAACGCTGTT 
0481 AACTTNCGCT TCGGCTACGA

in silico PCR Fragment

0001 CAGGAGGCCA GCATCTACAA GGCGCTGGCC CATCCGGCTC CCCCACTAGC CTATCCCCCG 
0061 CACGATGATG TGATCAGGGC CGCCAAGTTG TTGCGACAGG CCAAGCGACC CTTGGTGATC 
0121 GTGGGCAAGG GTTCCGCCTA CGCCCATGCC GAGAACACAC TGCGCCACTT CATCGAGAAC 
0181 ACCAACCTGC CCTTCCTCCC CACTCCCATG GGCAAGGGCG TTGTATCCGA TACGGCTCCG 
0241 CAGTGCGTCT CCTCGGCTCG CACCCTGGCC TTGCAAAAGG CCGATGTGGT GCTGCTCCTG 
0301 GGCGCTCGCC TCAACTGGAT TCTCCATTTC GGAAAGGCTC CACGCTACGA CAAGGACGTG 
0361 AAGTTTATCC AGGTGGACAT CAATCCTGAG GAGCTGCATA ACTCGGTTGT GGCTTCGGTG 
0421 GCCATCCAGG CGGACATCCG TCCATTCGCC GAGCAATTGT TTGAGCAAAT GAACGCTGTT 
0481 AACTTCCGCT TCGGCTACGA

Assemble Data


11208R-1.IR_full       1   CAGGAGGCCAGCATCTACAAGGCGCTGGCCCATCCGGCTCCCCCACTAGCCTATCCCCCG 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11208R-1.in silico     1   CAGGAGGCCAGCATCTACAAGGCGCTGGCCCATCCGGCTCCCCCACTAGCCTATCCCCCG 60


11208R-1.IR_full       61  CACGATGATGTGATCAGGGCCGCCAAGTTGTTGCGACAGGCCAAGCGACCCTTGGTGATC 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11208R-1.in silico     61  CACGATGATGTGATCAGGGCCGCCAAGTTGTTGCGACAGGCCAAGCGACCCTTGGTGATC 120


11208R-1.IR_full       121 GTGGGCAAGGGTTCCGCCTACGCCCATGCCGAGAACACACTGCGCCACTTCATCGAGAAC 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11208R-1.in silico     121 GTGGGCAAGGGTTCCGCCTACGCCCATGCCGAGAACACACTGCGCCACTTCATCGAGAAC 180


11208R-1.IR_full       181 ACCAACCTGCCCTTCCTCCCCACCCCCATGGACAAGGGCGTTGTATCCGATACGGCTCTG 240
                           ||||||||||||||||||||||| ||||||| |||||||||||||||||||||||||| |
11208R-1.in silico     181 ACCAACCTGCCCTTCCTCCCCACTCCCATGGGCAAGGGCGTTGTATCCGATACGGCTCCG 240


11208R-1.IR_full       241 CAGTGCGTCTCCTCGGCTCGCACCCTGGCCTTGCAAAAGGCCGATGTGGTGCTGCTCCTG 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11208R-1.in silico     241 CAGTGCGTCTCCTCGGCTCGCACCCTGGCCTTGCAAAAGGCCGATGTGGTGCTGCTCCTG 300


11208R-1.IR_full       301 GGCGCTCGCCTCAACTGGATTCTCCATTTCGGAAAGGTTCCGCGCTACGACAAGGACGTG 360
                           ||||||||||||||||||||||||||||||||||||| ||| ||||||||||||||||||
11208R-1.in silico     301 GGCGCTCGCCTCAACTGGATTCTCCATTTCGGAAAGGCTCCACGCTACGACAAGGACGTG 360


11208R-1.IR_full       361 AAGTTTATCCAGGTGGACATCAATCCTGAGGAGCTGCATAACTCGGTTGTGGCTTCGGTG 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11208R-1.in silico     361 AAGTTTATCCAGGTGGACATCAATCCTGAGGAGCTGCATAACTCGGTTGTGGCTTCGGTG 420


11208R-1.IR_full       421 GCCATCCAGGCGGACATCCGTCCATTCGCCGAGCAATTGTTTGAGCAAATGAACGCTGTT 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11208R-1.in silico     421 GCCATCCAGGCGGACATCCGTCCATTCGCCGAGCAATTGTTTGAGCAAATGAACGCTGTT 480


11208R-1.IR_full       481 AACTTNCGCTTCGGCTACGA 500
                           ||||| ||||||||||||||
11208R-1.in silico     481 AACTTCCGCTTCGGCTACGA 500

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	4	NM_137616.2	CG11208-RA (CG11208), mRNA
0	0	4	3	2	NM_167474.1	CG8909-RB (CG8909), mRNA
0	0	4	2	2	NM_078796.3	CG13106-RA (Or30a), mRNA
0	0	3	4	6	NM_132819.2	CG8105-RA (CG8105), mRNA
0	0	3	3	5	NM_206093.1	CG7736-RB, transcript variant B (Syx6), mRNA
0	0	3	3	5	NM_206094.1	CG7736-RE, transcript variant E (Syx6), mRNA
0	0	3	3	5	NM_206092.1	CG7736-RD, transcript variant D (Syx6), mRNA
0	0	3	2	3	NM_135299.2	CG7203-RA (CG7203), mRNA
0	0	2	3	7	NM_167634.1	CG7088-RB, transcript variant B (bnb), mRNA
0	0	2	3	7	NM_078682.2	CG7088-RC, transcript variant C (bnb), mRNA
0	0	2	3	7	NM_167633.1	CG7088-RA, transcript variant A (bnb), mRNA
0	0	2	3	7	NM_167635.1	CG7088-RD, transcript variant D (bnb), mRNA
0	0	2	2	9	NM_134552.3	CG1412-RA (RhoGAP19D), mRNA
0	0	1	7	10	NM_001014714.1	CG33513-RC, transcript variant C (Nmdar2), mRNA
0	0	1	4	4	NM_079507.2	CG2530-RA (corto), mRNA
0	0	1	4	3	NM_142989.3	CG6413-RA (Dis3), mRNA
0	0	1	4	2	NM_206242.1	CG32306-RD, transcript variant D (CG32306), mRNA
0	0	1	4	2	NM_139464.2	CG32306-RB, transcript variant B (CG32306), mRNA
0	0	1	4	2	NM_167948.1	CG32306-RA, transcript variant A (CG32306), mRNA
0	0	1	4	2	NM_167949.1	CG32306-RC, transcript variant C (CG32306), mRNA
0	0	1	3	4	NM_001014715.1	CG33513-RB, transcript variant B (Nmdar2), mRNA
0	0	1	3	4	NM_001014716.1	CG33513-RA, transcript variant A (Nmdar2), mRNA
0	0	1	3	4	NM_176443.1	CG33208-RE, transcript variant E (MICAL), mRNA
0	0	1	3	4	NM_176444.1	CG33208-RB, transcript variant B (MICAL), mRNA
0	0	1	3	4	NM_176447.1	CG33208-RF, transcript variant F (MICAL), mRNA
0	0	1	3	4	NM_176450.1	CG33208-RA, transcript variant A (MICAL), mRNA
0	0	1	3	4	NM_176446.1	CG33208-RD, transcript variant D (MICAL), mRNA
0	0	1	3	4	NM_176448.1	CG33208-RG, transcript variant G (MICAL), mRNA
0	0	1	3	4	NM_176449.1	CG33208-RH, transcript variant H (MICAL), mRNA
0	0	1	3	4	NM_176445.1	CG33208-RC, transcript variant C (MICAL), mRNA

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