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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 11201R-1 
 Symbol CG11201  Full Name CG11201 
 CG No CG11201  Old CG No CG11201 
 Synonyms CG11201 
 Accession No (Link to NCBI) NM_135224.1 
 Inserted Chr. lll 
 Insertional Mutation  lethal 
 Phenotype induced by Act5C-GAL4 at 28 degrees semi-lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgTTCACCGGAAATCGAAACCGC 
 Primer Seq. 3' 
aatctagaggtaccTCGGACATGCTCTGCAGCTTG 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 NTCACCGGAA ATCGAAACCG CTACTACAAC CCCGTCTCAA AGATCCAGTC GCTCATTCAC 
0061 AACCTGGNAC GCGGAGCTGG TGCAGCTGTG CAAGCAGTGC TCCGTGCAGA AGCCGCTGAC 
0121 TAGCCTCAAT AACAGCACCA GCCTTGGCAG CCACTTCTCG AATTTCGGAG CGCTTGGTGG 
0181 TGGCAGTGGG TCCAAAACCA TGGGCGGCAN GCNAGCAGCA TGGGTAGCAA CAACTTCTCT 
0241 CCCGACAGCA TTGGCAATCT TTTGGCCCGT GTCCGTGCAT CAACTCCGTT GCCCAGGACC 
0301 GTGACCAGCT CGCCCACGGC GCCGGAAGCA CAAAAGCGTC AGATGCGCAA TGTCTACCGC 
0361 ACCCGTGTGA TCGATGCGTA CCGTAATCGG CGGATCTTCA CCGTATACGG CAACTACCAC 
0421 ACGGTTAGAC GGGCTCTAAT GCGAAGGGGC TGGCTGGAGA AANCTGCCGG CCTCGCGGCA 
0481 TGCCAAGCTG CAGAGCATGT CCGA 
 in silico PCR Fragment 
0001 TTCACCGGAA ATCGAAACCG CTACTACAAC CCCGTCTCAA AGATCCAGTC GCTCATTCAC 
0061 AACCTGG-AC GCGGAGCTGG TGCAGCTGTG CAAGCAGTGC TCCGTGCAGA AGCCGCTGAC 
0121 TAGCCTCAAT AACAGCACCA GCCTTGGCAG CCACTTCTCG AATTTCGGAG CGCTTGGTGG 
0181 TGGCAGTGGG TCCAAAACCA TGGGCGGCA- GC-AGCAGCA TGGGTAGCAA CAACTTCTCT 
0241 CCCGACAGCA TTGGCAATCT TTTGGCCCGT GTCCGTGCAT CAACTCCGTT GCCCAGGACC 
0301 GTGACCAGCT CGCCCACGGC GCCGGAAGCA CAAAAGCGTC AGATGCGCAA TGTCTACCGC 
0361 ACCCGTGTGA TCGATGCGTA CCGTAATCGG CGGATCTTCA CCGTATACGG CAACTACCAC 
0421 ACGGTTAGAC GGGCTCTAAT GCGAAGGGGC TGGCTGGAGA AA-CTGCCGG CCTCGCGGCA 
0481 TGCCAAGCTG CAGAGCATGT CCGA 
 Assemble Data 

11201R-1.IR_full        1   TCACCGGAAATCGAAACCGCTACTACAACCCCGTCTCAAAGATCCAGTCGCTCATTCAC 59
                            |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11201R-1.in silico      1   TCACCGGAAATCGAAACCGCTACTACAACCCCGTCTCAAAGATCCAGTCGCTCATTCAC 59


11201R-1.IR_full       61  AACCTGGNACGCGGAGCTGGTGCAGCTGTGCAAGCAGTGCTCCGTGCAGAAGCCGCTGAC 119
                           ||||||| ||||||||||||||||||||||||||||||||||||||||||||||||||||
11201R-1.in silico     61  AACCTGG-ACGCGGAGCTGGTGCAGCTGTGCAAGCAGTGCTCCGTGCAGAAGCCGCTGAC 119


11201R-1.IR_full       121 TAGCCTCAATAACAGCACCAGCCTTGGCAGCCACTTCTCGAATTTCGGAGCGCTTGGTGG 179
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11201R-1.in silico     121 TAGCCTCAATAACAGCACCAGCCTTGGCAGCCACTTCTCGAATTTCGGAGCGCTTGGTGG 179


11201R-1.IR_full       181 TGGCAGTGGGTCCAAAACCATGGGCGGCANGCNAGCAGCATGGGTAGCAACAACTTCTCT 239
                           ||||||||||||||||||||||||||||| || |||||||||||||||||||||||||||
11201R-1.in silico     181 TGGCAGTGGGTCCAAAACCATGGGCGGCA-GC-AGCAGCATGGGTAGCAACAACTTCTCT 239


11201R-1.IR_full       241 CCCGACAGCATTGGCAATCTTTTGGCCCGTGTCCGTGCATCAACTCCGTTGCCCAGGACC 299
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11201R-1.in silico     241 CCCGACAGCATTGGCAATCTTTTGGCCCGTGTCCGTGCATCAACTCCGTTGCCCAGGACC 299


11201R-1.IR_full       301 GTGACCAGCTCGCCCACGGCGCCGGAAGCACAAAAGCGTCAGATGCGCAATGTCTACCGC 359
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11201R-1.in silico     301 GTGACCAGCTCGCCCACGGCGCCGGAAGCACAAAAGCGTCAGATGCGCAATGTCTACCGC 359


11201R-1.IR_full       361 ACCCGTGTGATCGATGCGTACCGTAATCGGCGGATCTTCACCGTATACGGCAACTACCAC 419
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11201R-1.in silico     361 ACCCGTGTGATCGATGCGTACCGTAATCGGCGGATCTTCACCGTATACGGCAACTACCAC 419


11201R-1.IR_full       421 ACGGTTAGACGGGCTCTAATGCGAAGGGGCTGGCTGGAGAAANCTGCCGGCCTCGCGGCA 479
                           |||||||||||||||||||||||||||||||||||||||||| |||||||||||||||||
11201R-1.in silico     421 ACGGTTAGACGGGCTCTAATGCGAAGGGGCTGGCTGGAGAAA-CTGCCGGCCTCGCGGCA 479


11201R-1.IR_full       481 TGCCAAGCTGCAGAGCATGTCCGA 503
                           ||||||||||||||||||||||||
11201R-1.in silico     481 TGCCAAGCTGCAGAGCATGTCCGA 503



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_135224.1  CG11201-RA (CG11201), mRNA 
0   0  4  5  8  NM_176386.1  CG11489-RC, transcript variant C (CG11489), mRNA 
0   0  4  4  4  NM_166663.1  CG13569-RB, transcript variant B (CG13569), mRNA 
0   0  4  4  4  NM_138044.2  CG13569-RA, transcript variant A (CG13569), mRNA 
0   0  4  4  4  NM_141130.3  CG11489-RB, transcript variant B (CG11489), mRNA 
0   0  4  3  2  NM_165069.1  CG8954-RB, transcript variant B (Smg5), mRNA 
0   0  4  3  2  NM_135841.4  CG8954-RA, transcript variant A (Smg5), mRNA 
0   0  4  2  17  NM_080364.3  CG5461-RA, transcript variant A (bun), mRNA 
0   0  4  2  17  NM_001042894.1  CG5461-RF, transcript variant F (bun), mRNA 
0   0  4  2  9  NM_165012.1  CG5461-RB, transcript variant B (bun), mRNA 
0   0  4  2  9  NM_165013.2  CG5461-RC, transcript variant C (bun), mRNA 
0   0  4  2  9  NM_001042892.1  CG5461-RD, transcript variant D (bun), mRNA 
0   0  4  2  9  NM_001042893.1  CG5461-RE, transcript variant E (bun), mRNA 
0   0  3  3  5  NM_141323.1  CG2097-RA (CG2097), mRNA 
0   0  3  2  7  NM_140829.2  CG6812-RA (CG6812), mRNA 
0   0  3  2  6  NM_167200.1  CG1343-RB, transcript variant B (Sp1), mRNA 
0   0  3  2  6  NM_132351.1  CG1343-RA, transcript variant A (Sp1), mRNA 
0   0  2  4  8  NM_166876.1  CG14622-RB, transcript variant B (DAAM), mRNA 
0   0  2  4  8  NM_166875.1  CG14622-RC, transcript variant C (DAAM), mRNA 
0   0  2  4  8  NM_130544.3  CG14622-RA, transcript variant A (DAAM), mRNA 
0   0  2  3  9  NM_001043216.1  CG34113-RO, transcript variant O (CG34113), mRNA 
0   0  2  3  5  NM_001043217.1  CG34113-RP, transcript variant P (CG34113), mRNA 
0   0  2  3  2  NM_144182.1  CG14162-RA (dpr6), mRNA 
0   0  2  2  6  NM_166022.1  CG30481-RA, transcript variant A (mRpL53), mRNA 
0   0  2  2  6  NM_001032246.1  CG33155-RD, transcript variant D (CG33155), mRNA 
0   0  2  2  6  NM_001032248.1  CG33155-RA, transcript variant A (CG33155), mRNA 
0   0  2  2  6  NM_001032247.1  CG33155-RC, transcript variant C (CG33155), mRNA 
0   0  2  2  5  NM_137255.2  CG15706-RA (CG15706), mRNA 
0   0  1  6  7  NM_137195.2  CG8180-RA (CG8180), mRNA 
0   0  1  5  11  NM_139967.1  CG7037-RB, transcript variant B (Cbl), mRNA 

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