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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
11190R-2 |
| Symbol |
CG11190 |
Full Name |
CG11190 |
| CG No |
CG11190 |
Old CG No |
CG11190 |
| Synonyms |
Dm*AAF46367/27-522, CG11190 |
| Accession No (Link to NCBI) |
NM_132247.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340
[
PubMed ID = 25473846
]
[
RRC reference
]
Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.
Zyxin links fat signaling to the hippo pathway.
PLoS Biol (2011) 9(6) e1000624
[
PubMed ID = 21666802
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgTCCGGTGGCGTTTGTCTTGCT |
| Primer Seq. 3' |
aatctagaggtaccCGAATCTTTGGCCATTGGCTG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 TCCGGTGGCG TNTTGTCTTG CTGCTGCTGG TTGCACCTAA TGCCGACGCG AATTTGGGAC
0061 GCGATGACGA ACGATTCCAC GAGGAGCTAG TGGTTCGTCC ATTGTCCGGC GACCATGTCA
0121 ACACCTACTT TCAGTTCACC ACGCGCTGGC ACTACGGCGA AAAGGATAAT CTTTACCACA
0181 CCCAATTGAC GCCTCGAGTA ATTGCGGAGC TGTTGCAGCA GTTTGCGGTA AAGGAGCTGC
0241 ACATTGGCCT AACCCAGGGA CTGTGGCGCT ACGAGACATG GGGCTATCCC ATTGTGGAGG
0301 CCACCAGCGG TGCCGAGNAT GTGGGCGTGG TTCAGTGGCG CAAATTTGAC CAACCGGGAT
0361 GTGGATCGCC AGTGGAAGGA GTTGGCCAAC GTTTTCTCGG GCGTGTTGTG CGCCTCGTTG
0421 AATTTCGTCG ACAATACGAA TAGCATTGCA CCGAGGCACT TGATTCGTCC ACAATTTATG
0481 CCAGCCAATG GCCAAAGATT CG |
| in silico PCR Fragment |
0001 TCCGGTGGCG T-TTGTCTTG CTGCTGCTGG TTGCACCTAA TGCCGACGCG AATTTGGGAC
0061 GCGATGACGA ACGATTCCAC GAGGAGCTAG TGGTTCGTCC ATTGTCCGGC GACCATGTCA
0121 ACACCTACTT TCAGTTCACC ACGCGCTGGC ACTACGGCGA AAAGGATAAT CTTTACCACA
0181 CCCAATTGAC GCCTCGAGTA ATTGCGGAGC TGTTGCAGCA GTTTGCGGTA AAGGAGCTGC
0241 ACATTGGCCT AACCCAGGGA CTGTGGCGCT ACGAGACATG GGGCTATCCC ATTGTGGAGG
0301 CCACCAGCGG TGCCGAG-AT GTGGGCGTGG TTCAGTGGCG CAAATTTGAC CAACCGGGAT
0361 GTGGATCGCC AGTGGAAGGA GTTGGCCAAC GTTTTCTCGG GCGTGTTGTG CGCCTCGTTG
0421 AATTTCGTCG ACAATACGAA TAGCATTGCA CCGAGGCACT TGATTCGTCC ACAATTTATG
0481 CCAGCCAATG GCCAAAGATT CG |
| Assemble Data |
11190R-2.IR_full 1 TCCGGTGGCGTNTTGTCTTGCTGCTGCTGGTTGCACCTAATGCCGACGCGAATTTGGGAC 60
||||||||||| ||||||||||||||||||||||||||||||||||||||||||||||||
11190R-2.in silico 1 TCCGGTGGCGT-TTGTCTTGCTGCTGCTGGTTGCACCTAATGCCGACGCGAATTTGGGAC 60
11190R-2.IR_full 61 GCGATGACGAACGATTCCACGAGGAGCTAGTGGTTCGTCCATTGTCCGGCGACCATGTCA 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11190R-2.in silico 61 GCGATGACGAACGATTCCACGAGGAGCTAGTGGTTCGTCCATTGTCCGGCGACCATGTCA 120
11190R-2.IR_full 121 ACACCTACTTTCAGTTCACCACGCGCTGGCACTACGGCGAAAAGGATAATCTTTACCACA 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11190R-2.in silico 121 ACACCTACTTTCAGTTCACCACGCGCTGGCACTACGGCGAAAAGGATAATCTTTACCACA 180
11190R-2.IR_full 181 CCCAATTGACGCCTCGAGTAATTGCGGAGCTGTTGCAGCAGTTTGCGGTAAAGGAGCTGC 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11190R-2.in silico 181 CCCAATTGACGCCTCGAGTAATTGCGGAGCTGTTGCAGCAGTTTGCGGTAAAGGAGCTGC 240
11190R-2.IR_full 241 ACATTGGCCTAACCCAGGGACTGTGGCGCTACGAGACATGGGGCTATCCCATTGTGGAGG 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11190R-2.in silico 241 ACATTGGCCTAACCCAGGGACTGTGGCGCTACGAGACATGGGGCTATCCCATTGTGGAGG 300
11190R-2.IR_full 301 CCACCAGCGGTGCCGAGNATGTGGGCGTGGTTCAGTGGCGCAAATTTGACCAACCGGGAT 360
||||||||||||||||| ||||||||||||||||||||||||||||||||||||||||||
11190R-2.in silico 301 CCACCAGCGGTGCCGAG-ATGTGGGCGTGGTTCAGTGGCGCAAATTTGACCAACCGGGAT 360
11190R-2.IR_full 361 GTGGATCGCCAGTGGAAGGAGTTGGCCAACGTTTTCTCGGGCGTGTTGTGCGCCTCGTTG 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11190R-2.in silico 361 GTGGATCGCCAGTGGAAGGAGTTGGCCAACGTTTTCTCGGGCGTGTTGTGCGCCTCGTTG 420
11190R-2.IR_full 421 AATTTCGTCGACAATACGAATAGCATTGCACCGAGGCACTTGATTCGTCCACAATTTATG 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11190R-2.in silico 421 AATTTCGTCGACAATACGAATAGCATTGCACCGAGGCACTTGATTCGTCCACAATTTATG 480
11190R-2.IR_full 481 CCAGCCAATGGCCAAAGATTCG 502
||||||||||||||||||||||
11190R-2.in silico 481 CCAGCCAATGGCCAAAGATTCG 502
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
1 |
NM_132247.2 |
CG11190-RA (CG11190), mRNA |
| 0.41 |
2 |
2 |
2 |
6 |
NM_206483.1 |
CG11190-RA (CG11190), mRNA, sub-group O CG3143-RB, transcript variant B (foxo), mRNA |
| 0.41 |
2 |
2 |
2 |
6 |
NM_206482.1 |
CG11190-RA (CG11190), mRNA, sub-group O CG3143-RB, transcript variant B (foxo), mRNA, sub-group O CG3143-RC, transcript variant C (foxo), mRNA |
| 0.41 |
2 |
2 |
2 |
6 |
NM_142073.3 |
CG11190-RA (CG11190), mRNA, sub-group O CG3143-RB, transcript variant B (foxo), mRNA, sub-group O CG3143-RC, transcript variant C (foxo), mRNA, sub-group O CG3143-RA, transcript variant A (foxo), mRNA |
| 0.2 |
1 |
5 |
3 |
3 |
NM_001043109.1 |
CG34140-RA, transcript variant A (CG34140), mRNA |
| 0.2 |
1 |
5 |
3 |
3 |
NM_001043107.1 |
CG34099-RB, transcript variant B (Mkp), mRNA |
| 0 |
0 |
6 |
4 |
3 |
NM_137337.1 |
CG9640-RA (CG9640), mRNA |
| 0 |
0 |
3 |
2 |
5 |
NM_170070.1 |
CG17077-RC, transcript variant C (pnt), mRNA |
| 0 |
0 |
3 |
2 |
5 |
NM_170651.3 |
CG17077-RD, transcript variant D (pnt), mRNA |
| 0 |
0 |
3 |
2 |
5 |
NM_079737.2 |
CG17077-RB, transcript variant B (pnt), mRNA |
| 0 |
0 |
3 |
2 |
2 |
NM_169453.1 |
CG10095-RA (dpr15), mRNA |
| 0 |
0 |
2 |
6 |
3 |
NM_001043069.1 |
CG34141-RA (CG34141), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_169913.1 |
CG3856-RA, transcript variant A (Oamb), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_169914.1 |
CG3856-RC, transcript variant C (Oamb), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_001032408.1 |
CG33956-RD, transcript variant D (kay), mRNA |
| 0 |
0 |
2 |
2 |
11 |
NM_169170.1 |
CG1070-RC, transcript variant C (Alh), mRNA |
| 0 |
0 |
1 |
5 |
3 |
NM_140524.2 |
CG12301-RA (CG12301), mRNA |
| 0 |
0 |
1 |
4 |
10 |
NM_140669.2 |
CG9715-RA (CG9715), mRNA |
| 0 |
0 |
1 |
4 |
4 |
NM_135363.2 |
CG7806-RA (CG7806), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_141338.1 |
CG2023-RA (CG2023), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_057701.3 |
CG5820-RD, transcript variant D (Gp150), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_166529.1 |
CG5820-RC, transcript variant C (Gp150), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_166528.1 |
CG5820-RB, transcript variant B (Gp150), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_166527.1 |
CG5820-RA, transcript variant A (Gp150), mRNA |
| 0 |
0 |
1 |
3 |
12 |
NM_078797.2 |
CG13109-RA (tai), mRNA |
| 0 |
0 |
1 |
3 |
7 |
NM_136154.1 |
CG10631-RA (CG10631), mRNA |
| 0 |
0 |
1 |
3 |
7 |
NM_135954.1 |
CG13260-RA (CG13260), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_137736.1 |
CG15666-RA (CG15666), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_141374.1 |
CG1154-RA (Osi12), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_169609.1 |
CG31304-RA (CG31304), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
 |
JNK signal
| N |
 |
| ++ |
|
| DB |
 |
| ++ |
|
| DC |
 |
| ++ |
|
| DM |
 |
| + |
|
| VM |
 |
| |
|
| VB |
 |
| |
|
|
Caspase-3 signal
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