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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
11152R-3 |
| Symbol |
CG11152 |
Full Name |
CG11152 |
| CG No |
CG11152 |
Old CG No |
CG11152 |
| Synonyms |
Dmcg11152, CG11152 |
| Accession No (Link to NCBI) |
NM_143694.1 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
male lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Nitta Y, Yamazaki D, Sugie A, Hiroi M, Tabata T.
DISCO Interacting Protein 2 regulates axonal bifurcation and guidance of Drosophila mushroom body neurons.
Dev Biol (2017) 421(2) 233-244
[
PubMed ID = 27908785
]
[
RRC reference
]
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgCCAACATCTACCCACTTGGAA |
| Primer Seq. 3' |
aatctagaggtaccGTGCCCTTTGCCATTTGCACT |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 CCAACATCTA CCCACTTGGA ACGACAAGAA CTTCACAAGC TCAATCAACG ACGATGACTT
0061 TGGAACAATA CCGCCTCCAG CTGTATAACT ATGCACTCAA TATTGAACCG ACTACGCTGC
0121 CCCCAATATG GTGGGACGGG TGGCAGTGGA GCTAGTGCTC TTTGGTTGCA TTTAAGCCCC
0181 TACGGACACC ATGGCTCAGG CAGTCTTCCA TCAGTTAATC GAATGGCCGC TCTTTCAACA
0241 ATTTCCCTCT TCCCACAAAC GCAACGCATT TTTCAACCTG AGGAGCCTAA ACCGCAACAC
0301 AGCTATATTG GTTTGATTGC CATGGCCATA TTGAGCTCAA CTGATATGAA GTTGGTTCTC
0361 TCAGACATTT ATCAATATAT TTTGGATAAT TATCCTTACT TTAGAAGTCG GGGGCCGGGA
0421 TGGAGAAACT CCATCAGGCA CAATCTCTCT CTAAATGATT GCTTTATCAA ATCTGGACGA
0481 AGTGCAAATG GCAAAGGGCA C |
| in silico PCR Fragment |
0001 CCAACATCTA CCCACTTGGA ACGACAAGAA CTTCACAAGC TCAATCAACG ACGATGACTT
0061 TGGAACAATA CCGCCTCCAG CTGTATAACT ATGCACTCAA TATTGAA-CG ACTACGCTGC
0121 CCCCAATATG GTGGGACGGG TGGCAGTGGA GCTAGTGCTC CTTGGTTGCA TTTAAGCCCC
0181 TACGGACACC ATGGCTCAGG CAGTCTTCCA TCAGTTAATC GAATGGCCGC TCTTTCAACA
0241 ATTTCCCTCT TCCCACAAAC GCAACGCATT TTTCAACCTG AGGAGCCTAA ACCGCAACAC
0301 AGCTATATTG GTTTGATTGC CATGGCCATA TTGAGCTCAA CTGATATGAA GTTGGTTCTC
0361 TCAGACATTT ATCAATATAT TTTGGATAAT TATCCTTACT TTAGAAGTCG GGGGCCGGGA
0421 TGGAGAAACT CCATCAGGCA CAATCTCTCT CTAAATGATT GCTTTATCAA ATCTGGACGA
0481 AGTGCAAATG GCAAAGGGCA C |
| Assemble Data |
11152R-3.IR_full 1 CCAACATCTACCCACTTGGAACGACAAGAACTTCACAAGCTCAATCAACGACGATGACTT 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11152R-3.in silico 1 CCAACATCTACCCACTTGGAACGACAAGAACTTCACAAGCTCAATCAACGACGATGACTT 60
11152R-3.IR_full 61 TGGAACAATACCGCCTCCAGCTGTATAACTATGCACTCAATATTGAACCGACTACGCTGC 120
||||||||||||||||||||||||||||||||||||||||||||||| ||||||||||||
11152R-3.in silico 61 TGGAACAATACCGCCTCCAGCTGTATAACTATGCACTCAATATTGAA-CGACTACGCTGC 120
11152R-3.IR_full 121 CCCCAATATGGTGGGACGGGTGGCAGTGGAGCTAGTGCTCTTTGGTTGCATTTAAGCCCC 180
|||||||||||||||||||||||||||||||||||||||| |||||||||||||||||||
11152R-3.in silico 121 CCCCAATATGGTGGGACGGGTGGCAGTGGAGCTAGTGCTCCTTGGTTGCATTTAAGCCCC 180
11152R-3.IR_full 181 TACGGACACCATGGCTCAGGCAGTCTTCCATCAGTTAATCGAATGGCCGCTCTTTCAACA 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11152R-3.in silico 181 TACGGACACCATGGCTCAGGCAGTCTTCCATCAGTTAATCGAATGGCCGCTCTTTCAACA 240
11152R-3.IR_full 241 ATTTCCCTCTTCCCACAAACGCAACGCATTTTTCAACCTGAGGAGCCTAAACCGCAACAC 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11152R-3.in silico 241 ATTTCCCTCTTCCCACAAACGCAACGCATTTTTCAACCTGAGGAGCCTAAACCGCAACAC 300
11152R-3.IR_full 301 AGCTATATTGGTTTGATTGCCATGGCCATATTGAGCTCAACTGATATGAAGTTGGTTCTC 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11152R-3.in silico 301 AGCTATATTGGTTTGATTGCCATGGCCATATTGAGCTCAACTGATATGAAGTTGGTTCTC 360
11152R-3.IR_full 361 TCAGACATTTATCAATATATTTTGGATAATTATCCTTACTTTAGAAGTCGGGGGCCGGGA 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11152R-3.in silico 361 TCAGACATTTATCAATATATTTTGGATAATTATCCTTACTTTAGAAGTCGGGGGCCGGGA 420
11152R-3.IR_full 421 TGGAGAAACTCCATCAGGCACAATCTCTCTCTAAATGATTGCTTTATCAAATCTGGACGA 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11152R-3.in silico 421 TGGAGAAACTCCATCAGGCACAATCTCTCTCTAAATGATTGCTTTATCAAATCTGGACGA 480
11152R-3.IR_full 481 AGTGCAAATGGCAAAGGGCAC 501
|||||||||||||||||||||
11152R-3.in silico 481 AGTGCAAATGGCAAAGGGCAC 501
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_143694.1 |
CG11152-RA (CG11152), mRNA |
| 0.2 |
1 |
3 |
4 |
2 |
NM_058033.3 |
CG7581-RA (Bub3), mRNA |
| 0 |
0 |
3 |
4 |
4 |
NM_165618.1 |
CG8579-RA (Jon44E), mRNA |
| 0 |
0 |
3 |
2 |
2 |
NM_079090.2 |
CG3668-RA (fd59A), mRNA |
| 0 |
0 |
2 |
4 |
3 |
NM_166382.1 |
CG11055-RC, transcript variant C (CG11055), mRNA |
| 0 |
0 |
2 |
4 |
3 |
NM_166381.1 |
CG11055-RA, transcript variant A (CG11055), mRNA |
| 0 |
0 |
2 |
4 |
3 |
NM_137619.2 |
CG11055-RB, transcript variant B (CG11055), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_079508.2 |
CG2534-RA, transcript variant A (cno), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_169025.1 |
CG2534-RB, transcript variant B (cno), mRNA |
| 0 |
0 |
1 |
5 |
2 |
NM_137200.2 |
CG30467-RA (CG30467), mRNA |
| 0 |
0 |
1 |
4 |
4 |
NM_165689.1 |
CG1902-RC, transcript variant C (CG1902), mRNA |
| 0 |
0 |
1 |
4 |
4 |
NM_136663.2 |
CG1902-RA, transcript variant A (CG1902), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_132606.1 |
CG3754-RA (CG3754), mRNA |
| 0 |
0 |
1 |
2 |
7 |
NM_078974.2 |
CG7776-RA, transcript variant A (E(Pc)), mRNA |
| 0 |
0 |
1 |
2 |
7 |
NM_165836.1 |
CG7776-RB, transcript variant B (E(Pc)), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_168316.1 |
CG4821-RB, transcript variant B (Tequila), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_140031.1 |
CG4821-RA, transcript variant A (Tequila), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_168314.1 |
CG4821-RD, transcript variant D (Tequila), mRNA |
| 0 |
0 |
0 |
5 |
7 |
NM_164957.1 |
CG6181-RB, transcript variant B (CG6181), mRNA |
| 0 |
0 |
0 |
5 |
7 |
NM_135642.2 |
CG6181-RA, transcript variant A (CG6181), mRNA |
| 0 |
0 |
0 |
5 |
5 |
NM_141082.1 |
CG7166-RA (CG7166), mRNA |
| 0 |
0 |
0 |
5 |
5 |
NM_170079.2 |
CG31145-RA, transcript variant A (CG31145), mRNA |
| 0 |
0 |
0 |
5 |
5 |
NM_176547.1 |
CG31145-RB, transcript variant B (CG31145), mRNA |
| 0 |
0 |
0 |
5 |
3 |
NM_135350.2 |
CG8372-RA, transcript variant A (CG8372), mRNA |
| 0 |
0 |
0 |
5 |
3 |
NM_164800.1 |
CG8372-RB, transcript variant B (CG8372), mRNA |
| 0 |
0 |
0 |
5 |
2 |
NM_079087.2 |
CG3633-RA (mRpS29), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_164830.3 |
CG18405-RA, transcript variant A (Sema-1a), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_135399.2 |
CG18405-RB, transcript variant B (Sema-1a), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_001042883.1 |
CG18405-RC, transcript variant C (Sema-1a), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_138048.1 |
CG12240-RB, transcript variant B (DnaJ-60), mRNA |
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| Wing Disc |
GFP
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Puc
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Caspase
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DAPI
 |
Merge
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Figure
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|
JNK signal
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Caspase-3 signal
| N |
 |
| ++ |
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| DB |
 |
| + |
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| DC |
 |
| + |
|
| DM |
 |
| ++ |
|
| VM |
 |
| + |
|
| VB |
 |
| |
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