|
Notice:
|
|
Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
|
|
| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
|
| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
11123R-3 |
| Symbol |
CG11123 |
Full Name |
CG11123 |
| CG No |
CG11123 |
Old CG No |
CG11123 |
| Synonyms |
CG11123 |
| Accession No (Link to NCBI) |
NM_136432.1 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgCCAAGAAGAAGGGCAACCGCT |
| Primer Seq. 3' |
aatctagaggtaccGTGATCATCTGTGAAGTCCGC |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 CCAAGAAGAA GGGCAACCNC TTTATgCGGA ACGCCCAAGG GGTTCGCCAA GCAGGGGATA
0061 TTCGGCCGGG GTACGCACAT CGACGACGAG CAGTTCAACT ACTTCATCAA CATTCTGGAT
0121 GCTATGAAAG TGGGATTCGA GGACGTAGAG GAGCGAGTCA TCATGGCCAA CAATGTGTTC
0181 GAGCAGACCC ACGATCAGGA GATCCATCTG GCCTCCAATC AGATCGTGTC CAAGGCTCTG
0241 GAGTCGTTAG TGGGCTTCGT GGACGACGTG CAGTTGGAGC GCTTTTTTAG CAAGTTTGGC
0301 GACAAcCTCC GGCCCATGTG CTCCGATCGG TTTGCCTCGC ACGTCCTGCA GAAAATGCTG
0361 GAAATCGCCT TTTTGCGCGG AGTGGGAAAA TCTGCAGCCC AGGATACCAG CGATGcNGNT
0421 AGCGCCAACA AAAAAGCTAA GCCGGATGCC GCTCAAGAGG AGGAGGAGTA CAACCTGCAG
0481 GCGGACTTCA CAGATGATCA C |
| in silico PCR Fragment |
0001 CCAAGAAGAA GGGCAACCGC TTTATGCGGA ACG-CCAAGG GGTTCGCCAA GCAGGGGATA
0061 TTCGGCCGGG GTACGCACAT CGACGACGAG CAGTTCAACT ACTTCATCAA CATTCTGGAT
0121 GCTATGAAAG TGGGATTCGA GGACGTAGAG GAGCGAGTCA TCATGGCCAA CAATGTGTTC
0181 GAGCAGACCC ACGATCAGGA GATCCATCTG GCCTCCAATC AGATCGTGTC CAAGGCTCTG
0241 GAGTCGTTAG TGGGCTTCGT GGACGACGTG CAGTTGGAGC GCTTTTTTAG CAAGTTTGGC
0301 GACAACCTCC GGCCCATGTG CTCCGATCGG TTTGCCTCGC ACGTCCTGCA GAAAATGCTG
0361 GAAATCGCCT TTTTGCGCGG AGTGGGAAAA TCTGCAGCCC AGGATACCAG CGATGCAGCT
0421 AGCGCCAACA AAAAAGCTAA GCCGGATGCC GCTCAAGAGG AGGAGGAGTA CAACCTGCAG
0481 GCGGACTTCA CAGATGATCA C |
| Assemble Data |
11123R-3.IR_full 1 CCAAGAAGAAGGGCAACCNCTTTATGCGGAACGCCCAAGGGGTTCGCCAAGCAGGGGATA 60
|||||||||||||||||| |||||||||||||| ||||||||||||||||||||||||||
11123R-3.in silico 1 CCAAGAAGAAGGGCAACCGCTTTATGCGGAACG-CCAAGGGGTTCGCCAAGCAGGGGATA 60
11123R-3.IR_full 61 TTCGGCCGGGGTACGCACATCGACGACGAGCAGTTCAACTACTTCATCAACATTCTGGAT 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11123R-3.in silico 61 TTCGGCCGGGGTACGCACATCGACGACGAGCAGTTCAACTACTTCATCAACATTCTGGAT 120
11123R-3.IR_full 121 GCTATGAAAGTGGGATTCGAGGACGTAGAGGAGCGAGTCATCATGGCCAACAATGTGTTC 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11123R-3.in silico 121 GCTATGAAAGTGGGATTCGAGGACGTAGAGGAGCGAGTCATCATGGCCAACAATGTGTTC 180
11123R-3.IR_full 181 GAGCAGACCCACGATCAGGAGATCCATCTGGCCTCCAATCAGATCGTGTCCAAGGCTCTG 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11123R-3.in silico 181 GAGCAGACCCACGATCAGGAGATCCATCTGGCCTCCAATCAGATCGTGTCCAAGGCTCTG 240
11123R-3.IR_full 241 GAGTCGTTAGTGGGCTTCGTGGACGACGTGCAGTTGGAGCGCTTTTTTAGCAAGTTTGGC 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11123R-3.in silico 241 GAGTCGTTAGTGGGCTTCGTGGACGACGTGCAGTTGGAGCGCTTTTTTAGCAAGTTTGGC 300
11123R-3.IR_full 301 GACAACCTCCGGCCCATGTGCTCCGATCGGTTTGCCTCGCACGTCCTGCAGAAAATGCTG 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11123R-3.in silico 301 GACAACCTCCGGCCCATGTGCTCCGATCGGTTTGCCTCGCACGTCCTGCAGAAAATGCTG 360
11123R-3.IR_full 361 GAAATCGCCTTTTTGCGCGGAGTGGGAAAATCTGCAGCCCAGGATACCAGCGATGCNGNT 420
|||||||||||||||||||||||||||||||||||||||||||||||||||||||| | |
11123R-3.in silico 361 GAAATCGCCTTTTTGCGCGGAGTGGGAAAATCTGCAGCCCAGGATACCAGCGATGCAGCT 420
11123R-3.IR_full 421 AGCGCCAACAAAAAAGCTAAGCCGGATGCCGCTCAAGAGGAGGAGGAGTACAACCTGCAG 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11123R-3.in silico 421 AGCGCCAACAAAAAAGCTAAGCCGGATGCCGCTCAAGAGGAGGAGGAGTACAACCTGCAG 480
11123R-3.IR_full 481 GCGGACTTCACAGATGATCAC 501
|||||||||||||||||||||
11123R-3.in silico 481 GCGGACTTCACAGATGATCAC 501
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_136432.1 |
CG11123-RA (CG11123), mRNA |
| 0.2 |
1 |
3 |
5 |
3 |
NM_176103.1 |
CG14764-RA (CG14764), mRNA |
| 0 |
0 |
4 |
4 |
2 |
NM_135222.2 |
CG11188-RA (CG11188), mRNA |
| 0 |
0 |
3 |
4 |
9 |
NM_137252.2 |
CG8445-RA, transcript variant A (CG8445), mRNA |
| 0 |
0 |
3 |
4 |
9 |
NM_176194.1 |
CG8445-RB, transcript variant B (CG8445), mRNA |
| 0 |
0 |
3 |
4 |
2 |
NM_001038789.1 |
CG34011-RA (CG34011), mRNA |
| 0 |
0 |
2 |
5 |
2 |
NM_139448.2 |
CG32300-RB (oxt), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_138025.2 |
CG3121-RA (CG3121), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_143526.1 |
CG9737-RA (CG9737), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_140119.2 |
CG6685-RA (CG6685), mRNA |
| 0 |
0 |
1 |
5 |
3 |
NM_079393.2 |
CG4083-RA (Mo25), mRNA |
| 0 |
0 |
1 |
4 |
5 |
NM_079617.3 |
CG7855-RA (timeout), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_140889.1 |
CG14101-RA (CG14101), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_140029.2 |
CG4942-RA (CG4942), mRNA |
| 0 |
0 |
1 |
2 |
9 |
NM_206212.1 |
CG3318-RB, transcript variant B (Dat), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_136985.2 |
CG13320-RA, transcript variant A (Sans), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_176160.1 |
CG13320-RB, transcript variant B (Sans), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_057796.2 |
CG10379-RA (mbc), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_167891.2 |
CG32313-RA (CG32313), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_166546.2 |
CG30092-RD, transcript variant D (jbug), mRNA |
| 0 |
0 |
0 |
5 |
6 |
NM_139450.2 |
CG5714-RA (ecd), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_141568.2 |
CG9797-RA (CG9797), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_001014610.1 |
CG33546-RB, transcript variant B (gfzf), mRNA |
| 0 |
0 |
0 |
5 |
3 |
NM_001014609.1 |
CG33546-RC, transcript variant C (gfzf), mRNA |
| 0 |
0 |
0 |
4 |
12 |
NM_001014571.1 |
CG33556-RA (form3), mRNA |
| 0 |
0 |
0 |
4 |
9 |
NM_132622.1 |
CG18646-RA (CG18646), mRNA |
| 0 |
0 |
0 |
4 |
7 |
NM_142992.1 |
CG6454-RB, transcript variant B (CG6454), mRNA |
| 0 |
0 |
0 |
4 |
7 |
NM_139356.1 |
CG3524-RA (v(2)k05816), mRNA |
| 0 |
0 |
0 |
4 |
6 |
NM_132186.1 |
CG10932-RA (CG10932), mRNA |
| 0 |
0 |
0 |
4 |
6 |
NM_001014461.1 |
CG9967-RB, transcript variant B (CG9967), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
 |
|
JNK signal
|
Caspase-3 signal
|
|
|
|
|
