NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

11094R-9

Symbol

dsx

Full Name

doublesex

CG No

CG11094

Old CG No

CG11094

Synonyms

Dmdsx, Dsx, ix-62c, Hr, CG11094, dsx

Accession No (Link to NCBI)

NM_169202.1

Inserted Chr.

Insertional Mutation

3 lethal

Phenotype induced by Act5C-GAL4 at 28 degrees

viable

Map Viewer

Reference
[Please submit your publication]

Cho Y, Lai CM, Lin KY, Hsu HJ.
A Targeted RNAi Screen Reveals Drosophila Female-Sterile Genes That Control the Size of Germline Stem Cell Niche During Development.
G3 (Bethesda) (2018) 8(7) 2345-2354 [ PubMed ID = 29764959 ] [ RRC reference ]

Nitta Y, Yamazaki D, Sugie A, Hiroi M, Tabata T.
DISCO Interacting Protein 2 regulates axonal bifurcation and guidance of Drosophila mushroom body neurons.
Dev Biol (2017) 421(2) 233-244 [ PubMed ID = 27908785 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGGACACCACTCGCATCACGGA

Primer Seq. 3'

aatctagaggtaccACCGTGGTCATGTGGTGATCG

Predicted Fragment Size

IR fragment full Seq

0001 GGACACCACT CGCATCACGG ACATGTCCTG CACCACCAGC AGGCAGCGGC GGCCGCAGCA 
0061 GCAGCTCCGT CGGCTCCAGC CTCCCATCTG GGTGGCTCCA GCACGGCCGC TTCCAGCATC 
0121 CACGGTCACG CCCACGCGCA CCACGTCCAC ATGGCAGCTG CTGCAGCCGC CTCGGTGGCT 
0181 CAGCACCAGC ACCAAAGCCA CCCACACTCG CACCACCACC ACCACCAGAA CCACCACCAG 
0241 CATCCGCATC AGCAACCGGC CACGCAGACC GCCCTGCGAT CTCCGCCGCA CAGCGACCAT 
0301 GGTGGCAGTG TGGGTCCGGC CACCAGCAGC TCCGGCGGTG GAGCACCCAG TTCCAGCAAT 
0361 GCGGCAGCGG CCACCTCGAG CAACGGATCC AGCGGCGGTG GAGGAGGAGG CGGAGGGGGC 
0421 AGCTCGGGTG GCGGGGCAGG AGGTGGTAGG TCATCGGGAA CATCGGTGAT CACTAGCGCC 
0481 GATCACCACA TGACCACGGT

in silico PCR Fragment

0001 GGACACCACT CGCATCACGG ACATGTCCTG CACCACCAGC AGGCAGCGGC GGCCGCAGCA 
0061 GCAGCTCCGT CGGCTCCAGC CTCCCATCTG GGTGGCTCCA GCACGGCCGC TTCCAGCATC 
0121 CACGGTCACG CCCACGCGCA CCACGTCCAC ATGGCAGCTG CTGCAGCCGC CTCGGTGGCT 
0181 CAGCACCAGC ACCAAAGCCA CCCACACTCG CACCACCACC ACCACCAGAA CCACCACCAG 
0241 CATCCGCATC AGCAACCGGC CACGCAGACC GCCCTGCGAT CTCCGCCGCA CAGCGACCAT 
0301 GGTGGCAGTG TGGGTCCGGC CACCAGCAGC TCCGGCGGTG GAGCACCCAG TTCCAGCAAT 
0361 GCGGCAGCGG CCACCTCGAG CAACGGATCC AGCGGCGGTG GAGGAGGAGG CGGAGGGGGC 
0421 AGCTCGGGTG GCGGGGCAGG AGGTGGTAGG TCATCGGGAA CATCGGTGAT CACTAGCGCC 
0481 GATCACCACA TGACCACGGT

Assemble Data


11094R-9.IR_full       1   GGACACCACTCGCATCACGGACATGTCCTGCACCACCAGCAGGCAGCGGCGGCCGCAGCA 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11094R-9.in silico     1   GGACACCACTCGCATCACGGACATGTCCTGCACCACCAGCAGGCAGCGGCGGCCGCAGCA 60


11094R-9.IR_full       61  GCAGCTCCGTCGGCTCCAGCCTCCCATCTGGGTGGCTCCAGCACGGCCGCTTCCAGCATC 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11094R-9.in silico     61  GCAGCTCCGTCGGCTCCAGCCTCCCATCTGGGTGGCTCCAGCACGGCCGCTTCCAGCATC 120


11094R-9.IR_full       121 CACGGTCACGCCCACGCGCACCACGTCCACATGGCAGCTGCTGCAGCCGCCTCGGTGGCT 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11094R-9.in silico     121 CACGGTCACGCCCACGCGCACCACGTCCACATGGCAGCTGCTGCAGCCGCCTCGGTGGCT 180


11094R-9.IR_full       181 CAGCACCAGCACCAAAGCCACCCACACTCGCACCACCACCACCACCAGAACCACCACCAG 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11094R-9.in silico     181 CAGCACCAGCACCAAAGCCACCCACACTCGCACCACCACCACCACCAGAACCACCACCAG 240


11094R-9.IR_full       241 CATCCGCATCAGCAACCGGCCACGCAGACCGCCCTGCGATCTCCGCCGCACAGCGACCAT 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11094R-9.in silico     241 CATCCGCATCAGCAACCGGCCACGCAGACCGCCCTGCGATCTCCGCCGCACAGCGACCAT 300


11094R-9.IR_full       301 GGTGGCAGTGTGGGTCCGGCCACCAGCAGCTCCGGCGGTGGAGCACCCAGTTCCAGCAAT 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11094R-9.in silico     301 GGTGGCAGTGTGGGTCCGGCCACCAGCAGCTCCGGCGGTGGAGCACCCAGTTCCAGCAAT 360


11094R-9.IR_full       361 GCGGCAGCGGCCACCTCGAGCAACGGATCCAGCGGCGGTGGAGGAGGAGGCGGAGGGGGC 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11094R-9.in silico     361 GCGGCAGCGGCCACCTCGAGCAACGGATCCAGCGGCGGTGGAGGAGGAGGCGGAGGGGGC 420


11094R-9.IR_full       421 AGCTCGGGTGGCGGGGCAGGAGGTGGTAGGTCATCGGGAACATCGGTGATCACTAGCGCC 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11094R-9.in silico     421 AGCTCGGGTGGCGGGGCAGGAGGTGGTAGGTCATCGGGAACATCGGTGATCACTAGCGCC 480


11094R-9.IR_full       481 GATCACCACATGACCACGGT 500
                           ||||||||||||||||||||
11094R-9.in silico     481 GATCACCACATGACCACGGT 500

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	40	NM_169202.1	CG11094-RA, transcript variant A (dsx), mRNA
100	482	0	0	32	NM_169203.1	CG11094-RB, transcript variant B (dsx), mRNA
100	482	0	0	32	NM_079548.4	CG11094-RC, transcript variant C (dsx), mRNA
1.24	6	4	3	15	NM_132837.1	CG8944-RB, transcript variant B (CG8944), mRNA
1.24	6	4	3	15	NM_167460.1	CG8944-RA, transcript variant A (CG8944), mRNA
1.03	5	4	39	65	NM_138223.1	CG12502-RA (CG12502), mRNA
0.82	4	4	28	114	NM_132661.1	CG12726-RA (CG12726), mRNA
0.62	3	3	2	26	NM_137195.2	CG8180-RA (CG8180), mRNA
0.41	2	6	6	43	NM_175956.1	CG33003-RA (CG33003), mRNA
0.41	2	4	12	38	NM_138050.2	CG3363-RA (CG3363), mRNA
0.2	1	8	12	54	NM_169935.1	CG31191-RA (CG31191), mRNA
0.2	1	6	12	28	NM_137123.2	CG17390-RA (CG17390), mRNA
0.2	1	6	9	39	NM_131939.4	CG17592-RB, transcript variant B (Usf), mRNA
0.2	1	6	9	39	NM_167010.1	CG17592-RA, transcript variant A (Usf), mRNA
0.2	1	6	8	35	NM_165841.1	CG9015-RB, transcript variant B (en), mRNA
0.2	1	6	8	35	NM_078976.3	CG9015-RA, transcript variant A (en), mRNA
0.2	1	5	13	30	NM_206782.1	CG6103-RD, transcript variant D (CrebB-17A), mRNA
0.2	1	5	13	30	NM_206784.1	CG6103-RE, transcript variant E (CrebB-17A), mRNA
0.2	1	5	13	29	NM_206781.1	CG6103-RF, transcript variant F (CrebB-17A), mRNA
0.2	1	5	13	29	NM_206783.1	CG6103-RG, transcript variant G (CrebB-17A), mRNA
0.2	1	4	16	31	NM_140932.1	CG6996-RA (CG6996), mRNA
0.2	1	3	26	72	NM_080362.2	CG31868-RA (Samuel), mRNA
0.2	1	3	24	78	NM_167189.2	CG32705-RA (CG32705), mRNA
0.2	1	3	15	80	NM_136593.1	CG8181-RA (CG8181), mRNA
0.2	1	3	13	74	NM_167647.2	CG12199-RB, transcript variant B (kek5), mRNA
0.2	1	3	13	74	NM_133154.2	CG12199-RA, transcript variant A (kek5), mRNA
0.2	1	3	11	27	NM_170531.3	CG1447-RA, transcript variant A (Ptx1), mRNA
0.2	1	3	11	27	NM_206591.1	CG1447-RC, transcript variant C (Ptx1), mRNA
0.2	1	2	17	47	NM_132175.2	CG12155-RA (CG12155), mRNA
0.2	1	2	14	42	NM_080310.2	CG7952-RB (gt), mRNA

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