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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID

11024R-2

Symbol

Full Name

clot

CG No

CG11024

Old CG No

CG11024

Synonyms

CG11024, BcDNA:RE06767, cl

Accession No (Link to NCBI)

NM_135086.2

Inserted Chr.

lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees

viable

Map Viewer

Reference
[Please submit your publication]

Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgTGGTGGTCACACACAACGTCA

Primer Seq. 3'

aatctagaggtaccTCCTCATCCTCGAACATCATC

Predicted Fragment Size

IR fragment full Seq

0001 TGGTGGTCAC ACACAACGTC AAGGGATACG ATGAGTTCAC CAAAAAGATG GAGGAACTGG 
0061 AGAACGGCGA CGAGCCGGTC CACGTCCTCT TCAGTGGCGG CAAGGACGAG AAAGGAGAGA 
0121 GCTGGTGCCC CTACTGCGTC AAGGCGGAGC CGGTGATACA CGATGCGCTG AAGAAGGCCC 
0181 CGGGCAACTC GCACTTCGTG CATGTGGATG TGGGCGAGCG GGCATACTGG AAGGATTTGA 
0241 ACTGCCCCTT CCGCAAGGAT CCCAACACGC ATCTGATCTT CTTGCCCACT CTGTTGCGCT 
0301 GGAAGCGGCC ACAGCGTCTG GATGGCGAGC GCTGCTCCAA TCAGGATCTC GTCGAGATGA 
0361 TGTTCGAGGA TGAGGA

in silico PCR Fragment

0001 TGGTGGTCAC ACACAACGTC AAGGGATACG ATGAGTTCAC CAAAAAGATG GAGGAACTGG 
0061 AGAACGGCGA CGAGCCGGTC CACGTCCTCT TCAGTGGCGG CAAGGACGAG AAAGGAGAGA 
0121 GCTGGTGCCC CTACTGCGTC AAGGCGGAGC CGGTGATACA CGATGCGCTG AAGAAGGCCC 
0181 CGGGCAACTC GCACTTCGTG CATGTGGATG TGGGCGAGCG GGCATACTGG AAGGATTTGA 
0241 ACTGCCCCTT CCGCAAGGAT CCCAACACGC ATCTGATCTT CTTGCCCACT CTGTTGCGCT 
0301 GGAAGCGGCC ACAGCGTCTG GATGGCGAGC GCTGCTCCAA TCAGGATCTC GTCGAGATGA 
0361 TGTTCGAGGA TGAGGA

Assemble Data


11024R-2.IR_full       1   TGGTGGTCACACACAACGTCAAGGGATACGATGAGTTCACCAAAAAGATGGAGGAACTGG 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11024R-2.in silico     1   TGGTGGTCACACACAACGTCAAGGGATACGATGAGTTCACCAAAAAGATGGAGGAACTGG 60


11024R-2.IR_full       61  AGAACGGCGACGAGCCGGTCCACGTCCTCTTCAGTGGCGGCAAGGACGAGAAAGGAGAGA 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11024R-2.in silico     61  AGAACGGCGACGAGCCGGTCCACGTCCTCTTCAGTGGCGGCAAGGACGAGAAAGGAGAGA 120


11024R-2.IR_full       121 GCTGGTGCCCCTACTGCGTCAAGGCGGAGCCGGTGATACACGATGCGCTGAAGAAGGCCC 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11024R-2.in silico     121 GCTGGTGCCCCTACTGCGTCAAGGCGGAGCCGGTGATACACGATGCGCTGAAGAAGGCCC 180


11024R-2.IR_full       181 CGGGCAACTCGCACTTCGTGCATGTGGATGTGGGCGAGCGGGCATACTGGAAGGATTTGA 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11024R-2.in silico     181 CGGGCAACTCGCACTTCGTGCATGTGGATGTGGGCGAGCGGGCATACTGGAAGGATTTGA 240


11024R-2.IR_full       241 ACTGCCCCTTCCGCAAGGATCCCAACACGCATCTGATCTTCTTGCCCACTCTGTTGCGCT 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11024R-2.in silico     241 ACTGCCCCTTCCGCAAGGATCCCAACACGCATCTGATCTTCTTGCCCACTCTGTTGCGCT 300


11024R-2.IR_full       301 GGAAGCGGCCACAGCGTCTGGATGGCGAGCGCTGCTCCAATCAGGATCTCGTCGAGATGA 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
11024R-2.in silico     301 GGAAGCGGCCACAGCGTCTGGATGGCGAGCGCTGCTCCAATCAGGATCTCGTCGAGATGA 360


11024R-2.IR_full       361 TGTTCGAGGATGAGGA 376
                           ||||||||||||||||
11024R-2.in silico     361 TGTTCGAGGATGAGGA 376

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	358	0	0	0	NM_135086.2	CG11024-RA (cl), mRNA
0	0	5	3	4	NM_170556.1	CG11525-RC, transcript variant C (CycG), mRNA
0	0	5	3	4	NM_170558.1	CG11525-RE, transcript variant E (CycG), mRNA
0	0	5	3	4	NM_079870.2	CG11525-RA, transcript variant A (CycG), mRNA
0	0	5	3	4	NM_170555.1	CG11525-RB, transcript variant B (CycG), mRNA
0	0	5	3	4	NM_170557.1	CG11525-RD, transcript variant D (CycG), mRNA
0	0	5	2	6	NM_176447.1	CG33208-RF, transcript variant F (MICAL), mRNA
0	0	5	2	6	NM_176445.1	CG33208-RC, transcript variant C (MICAL), mRNA
0	0	5	2	6	NM_176449.1	CG33208-RH, transcript variant H (MICAL), mRNA
0	0	5	2	6	NM_176448.1	CG33208-RG, transcript variant G (MICAL), mRNA
0	0	5	2	6	NM_176446.1	CG33208-RD, transcript variant D (MICAL), mRNA
0	0	5	2	3	NM_176444.1	CG33208-RB, transcript variant B (MICAL), mRNA
0	0	4	2	7	NM_136761.2	CG7614-RA (Mat1), mRNA
0	0	2	4	9	NM_144448.2	CG1915-RC, transcript variant C (sls), mRNA
0	0	2	4	8	NM_079937.2	CG1915-RA, transcript variant A (sls), mRNA
0	0	2	2	2	NM_141990.2	CG8063-RA (yellow-f2), mRNA
0	0	1	6	5	NM_135306.2	CG7144-RA (CG7144), mRNA
0	0	1	4	3	NM_132453.1	CG2076-RA (CG2076), mRNA
0	0	1	3	5	NM_136905.1	CG8854-RA (CG8854), mRNA
0	0	1	3	2	NM_057464.3	CG5779-RA (Bc), mRNA
0	0	1	2	4	NM_132832.2	CG8191-RA (CG8191), mRNA
0	0	1	2	4	NM_132833.1	CG12379-RA (CG12379), mRNA
0	0	1	2	3	NM_141676.2	CG12950-RA (CG12950), mRNA
0	0	1	2	2	NM_137921.1	CG30188-RA (CG30188), mRNA
0	0	0	5	6	NM_080175.2	CG12242-RA (GstD5), mRNA
0	0	0	5	5	NM_135685.2	CG6686-RB, transcript variant B (CG6686), mRNA
0	0	0	5	5	NM_057477.3	CG1034-RA, transcript variant A (bcd), mRNA
0	0	0	5	5	NM_137909.2	CG9882-RA (Art7), mRNA
0	0	0	5	5	NM_164977.1	CG6686-RA, transcript variant A (CG6686), mRNA
0	0	0	5	5	NM_169157.1	CG1034-RD, transcript variant D (bcd), mRNA

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