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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
10955R-2 |
| Symbol |
Rtf1 |
Full Name |
Rtf1 |
| CG No |
CG10955 |
Old CG No |
CG10955 |
| Synonyms |
Rtf1, dRtf1, CG10955 |
| Accession No (Link to NCBI) |
NM_137821.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
lethal |
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Saj A, Arziman Z, Stempfle D, van Belle W, Sauder U, Horn T, Dürrenberger M, Paro R, Boutros M, Merdes G.
A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network.
Dev Cell (2010) 18(5) 862-76
[
PubMed ID = 20493818
]
[
RRC reference
]
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| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGCAATCCCTGATCGACTCCAA |
| Primer Seq. 3' |
aatctagaggtaccGCCTTCGATCCTCATCATCGC |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 GCAATTCCCT GATCGACTCC AACTCCAGCG ATAGCGACAG CGAGTCCGAG ACCAATCTGG
0061 AATCGGACCT GATGTCGCTC GCCAAGAAGC GAAAGAAGCC CCAAACGGCG GCCAAGTCAA
0121 GTTNCCCGCT CCGACTCTGA TTCCGACTGG GCCAACAACA AAGCTGGAGC TCCCTCGTCC
0181 AAGAAGAAGA AGCGTCAGAA GCCCAGCAGA GACTCCAGCT CCTCGGAGTC CAATTGGGAT
0241 GACGACAGTC AGGATGAGAG GCAGCCAGCG AGGCAGAGTC CTGCACAGAC GCAACAGGAG
0301 CACAAGCCCC CGGAGCANGC CTCCCAGCNC GCCCAGcTCA GCGAGCAGGA GGAGGGCGAG
0361 GTCTCCGATA GCGATTCCGA CAAGTCCAAG TCCAACTCCT CCTCATCCGG CTCCGACTCC
0421 TCTAGTTCTT CCTCCAGCTC CGACTCGGAA TTTGACGACG GGTTCGATGA TGACCTCATG
0481 GGCGATGATG AGGATCGAAG GC |
| in silico PCR Fragment |
0001 GCAA-TCCCT GATCGACTCC AACTCCAGCG ATAGCGACAG CGAGTCCGAG ACCAATCTGG
0061 AATCGGACCT GATGTCGCTC GCCAAGAAGC GAAAGAAGCC CCAAACGGCG GCCAAGTCAA
0121 GTT-CCCGCT CCGACTCTGA TTCCGACTGG GCCAACAACA AAGCTGGAGC TCCCTCGTCC
0181 AAGAAGAAGA AGCGTCAGAA GCCCAGCAGA GACTCCAGCT CCTCGGAGTC CAATTGGGAT
0241 GACGACAGTC AGGATGAGAG GCAGCCAGCG AGGCAGAGTC CTGCACAGAC GCAACAGGAG
0301 CACAAGCCCC CGGAGCAGGC CTCCCAGCCC GCCCAGCTCA GCGAGCAGGA GGAGGGCGAG
0361 GTCTCCGATA GCGATTCCGA CAAGTCCAAG TCCAACTCCT CCTCATCCGG CTCCGACTCC
0421 TCTAGTTCTT CCTCCAGCTC CGACTCGGAA TTTGACGACG GGTTCGATGA TGACCTCATG
0481 GGCGATGATG AGGATCGAAG GC |
| Assemble Data |
10955R-2.IR_full 1 GCAATTCCCTGATCGACTCCAACTCCAGCGATAGCGACAGCGAGTCCGAGACCAATCTGG 60
|||| |||||||||||||||||||||||||||||||||||||||||||||||||||||||
10955R-2.in silico 1 GCAA-TCCCTGATCGACTCCAACTCCAGCGATAGCGACAGCGAGTCCGAGACCAATCTGG 60
10955R-2.IR_full 61 AATCGGACCTGATGTCGCTCGCCAAGAAGCGAAAGAAGCCCCAAACGGCGGCCAAGTCAA 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10955R-2.in silico 61 AATCGGACCTGATGTCGCTCGCCAAGAAGCGAAAGAAGCCCCAAACGGCGGCCAAGTCAA 120
10955R-2.IR_full 121 GTTNCCCGCTCCGACTCTGATTCCGACTGGGCCAACAACAAAGCTGGAGCTCCCTCGTCC 180
||| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10955R-2.in silico 121 GTT-CCCGCTCCGACTCTGATTCCGACTGGGCCAACAACAAAGCTGGAGCTCCCTCGTCC 180
10955R-2.IR_full 181 AAGAAGAAGAAGCGTCAGAAGCCCAGCAGAGACTCCAGCTCCTCGGAGTCCAATTGGGAT 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10955R-2.in silico 181 AAGAAGAAGAAGCGTCAGAAGCCCAGCAGAGACTCCAGCTCCTCGGAGTCCAATTGGGAT 240
10955R-2.IR_full 241 GACGACAGTCAGGATGAGAGGCAGCCAGCGAGGCAGAGTCCTGCACAGACGCAACAGGAG 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10955R-2.in silico 241 GACGACAGTCAGGATGAGAGGCAGCCAGCGAGGCAGAGTCCTGCACAGACGCAACAGGAG 300
10955R-2.IR_full 301 CACAAGCCCCCGGAGCANGCCTCCCAGCNCGCCCAGCTCAGCGAGCAGGAGGAGGGCGAG 360
||||||||||||||||| |||||||||| |||||||||||||||||||||||||||||||
10955R-2.in silico 301 CACAAGCCCCCGGAGCAGGCCTCCCAGCCCGCCCAGCTCAGCGAGCAGGAGGAGGGCGAG 360
10955R-2.IR_full 361 GTCTCCGATAGCGATTCCGACAAGTCCAAGTCCAACTCCTCCTCATCCGGCTCCGACTCC 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10955R-2.in silico 361 GTCTCCGATAGCGATTCCGACAAGTCCAAGTCCAACTCCTCCTCATCCGGCTCCGACTCC 420
10955R-2.IR_full 421 TCTAGTTCTTCCTCCAGCTCCGACTCGGAATTTGACGACGGGTTCGATGATGACCTCATG 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10955R-2.in silico 421 TCTAGTTCTTCCTCCAGCTCCGACTCGGAATTTGACGACGGGTTCGATGATGACCTCATG 480
10955R-2.IR_full 481 GGCGATGATGAGGATCGAAGGC 502
||||||||||||||||||||||
10955R-2.in silico 481 GGCGATGATGAGGATCGAAGGC 502
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
10 |
NM_137821.2 |
CG10955-RA (CG10955), mRNA |
| 0.2 |
1 |
2 |
3 |
8 |
NM_140401.1 |
CG10741-RB, transcript variant B (CG10741), mRNA |
| 0 |
0 |
6 |
6 |
7 |
NM_132159.1 |
CG1677-RA (CG1677), mRNA |
| 0 |
0 |
5 |
5 |
3 |
NM_057624.2 |
CG4698-RA (Wnt4), mRNA |
| 0 |
0 |
4 |
2 |
2 |
NM_168563.1 |
CG32137-RB, transcript variant B (CG32137), mRNA |
| 0 |
0 |
4 |
2 |
2 |
NM_168564.1 |
CG32137-RA, transcript variant A (CG32137), mRNA |
| 0 |
0 |
3 |
7 |
14 |
NM_169094.1 |
CG1093-RA, transcript variant A (plx), mRNA |
| 0 |
0 |
3 |
5 |
11 |
NM_001042868.1 |
CG34124-RA (CG34124), mRNA |
| 0 |
0 |
3 |
5 |
10 |
NM_169095.1 |
CG1093-RB, transcript variant B (plx), mRNA |
| 0 |
0 |
3 |
3 |
11 |
NM_001032407.1 |
CG33956-RA, transcript variant A (kay), mRNA |
| 0 |
0 |
3 |
2 |
6 |
NM_079540.4 |
CG2512-RA, transcript variant A (alphaTub84D), mRNA |
| 0 |
0 |
3 |
2 |
6 |
NM_169189.1 |
CG2512-RB, transcript variant B (alphaTub84D), mRNA |
| 0 |
0 |
2 |
8 |
10 |
NM_132521.2 |
CG1578-RA (CG1578), mRNA |
| 0 |
0 |
2 |
7 |
22 |
NM_167309.1 |
CG32663-RA (CG32663), mRNA |
| 0 |
0 |
2 |
4 |
7 |
NM_132262.2 |
CG11265-RA, transcript variant A (CG11265), mRNA |
| 0 |
0 |
2 |
4 |
7 |
NM_206658.1 |
CG11265-RE, transcript variant E (CG11265), mRNA |
| 0 |
0 |
2 |
4 |
7 |
NM_206660.1 |
CG11265-RB, transcript variant B (CG11265), mRNA |
| 0 |
0 |
2 |
4 |
7 |
NM_206659.1 |
CG11265-RC, transcript variant C (CG11265), mRNA |
| 0 |
0 |
2 |
4 |
7 |
NM_138223.1 |
CG12502-RA (CG12502), mRNA |
| 0 |
0 |
2 |
4 |
2 |
NM_166982.1 |
CG2872-RC, transcript variant C (AlstR), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_135807.2 |
CG10859-RA (CG10859), mRNA |
| 0 |
0 |
1 |
6 |
10 |
NM_079700.1 |
CG3723-RA (Dhc93AB), mRNA |
| 0 |
0 |
1 |
5 |
2 |
NM_078853.2 |
CG4767-RA (Tektin-A), mRNA |
| 0 |
0 |
1 |
4 |
10 |
NM_080333.2 |
CG3346-RA (pon), mRNA |
| 0 |
0 |
1 |
4 |
5 |
NM_138050.2 |
CG3363-RA (CG3363), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_078673.2 |
CG7128-RA (Taf8), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_079410.2 |
CG7354-RA (mRpS26), mRNA |
| 0 |
0 |
1 |
3 |
9 |
NM_137190.2 |
CG8174-RB, transcript variant B (SRPK), mRNA |
| 0 |
0 |
1 |
3 |
9 |
NM_166093.1 |
CG8174-RA, transcript variant A (SRPK), mRNA |
| 0 |
0 |
1 |
3 |
9 |
NM_135461.2 |
CG4364-RA (CG4364), mRNA |
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