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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
10947R-1 |
| Symbol |
CG10947 |
Full Name |
CG10947 |
| CG No |
CG10947 |
Old CG No |
CG10947 |
| Synonyms |
38C.42, CG10947 |
| Accession No (Link to NCBI) |
NM_136187.2 |
| Inserted Chr. |
lll |
| Insertional Mutation |
lethal |
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGAAGTGGGTGTGGCACAGTTG |
| Primer Seq. 3' |
aatctagaggtaccTACTCCGACTCGTTCTCCAGC |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 GAAGTGGGTG TGGCACAGTT GGCAACTATA TCGTTGCTAC TGCAGTACGA CATTGACAAC
0061 ACACTGAATG CGCTCAAGGA GCACAAGAAG ATGACGCTGG ACGCTCGATT GGGCGGCGAG
0121 GTgGCCGCCG CAGNTCAACA ACAACCAGGA CAAGGACATA AACCGCAATC TGCCGGAGCA
0181 GACCGAACTG GAGGAGGATC TGCGACTCAA GAGCGGCGAG AACGGCTTCA TGCCGCCCAC
0241 TCCGCCGGCG ACGCCCAACC TGCAGAGCAC TGCCCAGAAG CGCTGGAAGA TCCTGGCCAA
0301 GGTGCTGCGC AAGGATTCCG AGGAGACGGT GTCCTCGTCC AGCGATGAGT TCGCCGAGGA
0361 GCAAACTGCA TCCGTGCGGC GCTTTCAAGA GCTTCGATCT GCTCCGCCAG GACAGCTTCG
0421 AGGATCACGT GAGTCTTGAA GTGCTTGGGC AAGACGGAGA ACTGGTACAA ATACCGCATG
0481 CAGCTGGAGA ACGAGTCGGA GTA |
| in silico PCR Fragment |
0001 GAAGTGGGTG TGGCACAGTT GGCAACTATA TCGTTGCTAC TGCAGTACGA CATTGACAAC
0061 ACACTGAATG CGCTCAAGGA GCACAAGAAG ATGACGCTGG ACGCTCGATT GGGCGGCGAG
0121 GTGGCCGCCG CAG-TCAACA ACAACCAGGA CAAGGACATA AACCGCAATC TGCCGGAGCA
0181 GACCGAACTG GAGGAGGATC TGCGACTCAA GAGCGGCGAG AACGGCTTCA TGCCGCCCAC
0241 TCCGCCGGCG ACGCCCAACC TGCAGAGCAC TGCCCAGAAG CGCTGGAAGA TCCTGGCCAA
0301 GGTGCTGCGC AAGGATTCCG AGGAGACGGT GTCCTCGTCC AGCGATGAGT TCGCCGAGGA
0361 GCAAACTGCA TCCGTGCGGC GC-TTCAAGA GCTTCGATCT GCTCCGCCAG GACAGCTTCG
0421 AGGATCACGT GAGTC-TGAA GTGCTTGGGC AAGACGGAGA ACTGGTACAA ATACCGCATG
0481 CAGCTGGAGA ACGAGTCGGA GTA |
| Assemble Data |
10947R-1.IR_full 1 GAAGTGGGTGTGGCACAGTTGGCAACTATATCGTTGCTACTGCAGTACGACATTGACAAC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10947R-1.in silico 1 GAAGTGGGTGTGGCACAGTTGGCAACTATATCGTTGCTACTGCAGTACGACATTGACAAC 60
10947R-1.IR_full 61 ACACTGAATGCGCTCAAGGAGCACAAGAAGATGACGCTGGACGCTCGATTGGGCGGCGAG 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10947R-1.in silico 61 ACACTGAATGCGCTCAAGGAGCACAAGAAGATGACGCTGGACGCTCGATTGGGCGGCGAG 120
10947R-1.IR_full 121 GTGGCCGCCGCAGNTCAACAACAACCAGGACAAGGACATAAACCGCAATCTGCCGGAGCA 180
||||||||||||| ||||||||||||||||||||||||||||||||||||||||||||||
10947R-1.in silico 121 GTGGCCGCCGCAG-TCAACAACAACCAGGACAAGGACATAAACCGCAATCTGCCGGAGCA 180
10947R-1.IR_full 181 GACCGAACTGGAGGAGGATCTGCGACTCAAGAGCGGCGAGAACGGCTTCATGCCGCCCAC 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10947R-1.in silico 181 GACCGAACTGGAGGAGGATCTGCGACTCAAGAGCGGCGAGAACGGCTTCATGCCGCCCAC 240
10947R-1.IR_full 241 TCCGCCGGCGACGCCCAACCTGCAGAGCACTGCCCAGAAGCGCTGGAAGATCCTGGCCAA 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10947R-1.in silico 241 TCCGCCGGCGACGCCCAACCTGCAGAGCACTGCCCAGAAGCGCTGGAAGATCCTGGCCAA 300
10947R-1.IR_full 301 GGTGCTGCGCAAGGATTCCGAGGAGACGGTGTCCTCGTCCAGCGATGAGTTCGCCGAGGA 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10947R-1.in silico 301 GGTGCTGCGCAAGGATTCCGAGGAGACGGTGTCCTCGTCCAGCGATGAGTTCGCCGAGGA 360
10947R-1.IR_full 361 GCAAACTGCATCCGTGCGGCGCTTTCAAGAGCTTCGATCTGCTCCGCCAGGACAGCTTCG 420
|||||||||||||||||||||| |||||||||||||||||||||||||||||||||||||
10947R-1.in silico 361 GCAAACTGCATCCGTGCGGCGC-TTCAAGAGCTTCGATCTGCTCCGCCAGGACAGCTTCG 420
10947R-1.IR_full 421 AGGATCACGTGAGTCTTGAAGTGCTTGGGCAAGACGGAGAACTGGTACAAATACCGCATG 480
||||||||||||||| ||||||||||||||||||||||||||||||||||||||||||||
10947R-1.in silico 421 AGGATCACGTGAGTC-TGAAGTGCTTGGGCAAGACGGAGAACTGGTACAAATACCGCATG 480
10947R-1.IR_full 481 CAGCTGGAGAACGAGTCGGAGTA 503
|||||||||||||||||||||||
10947R-1.in silico 481 CAGCTGGAGAACGAGTCGGAGTA 503
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
5 |
NM_165328.1 |
CG10947-RB, transcript variant B (CG10947), mRNA |
| 100 |
482 |
0 |
0 |
5 |
NM_136187.2 |
CG10947-RA, transcript variant A (CG10947), mRNA |
| 100 |
482 |
0 |
0 |
5 |
NM_165329.1 |
CG10947-RC, transcript variant C (CG10947), mRNA |
| 0.41 |
2 |
3 |
3 |
2 |
NM_166951.1 |
CG2714-RB, transcript variant B (crm), mRNA |
| 0.41 |
2 |
3 |
3 |
2 |
NM_057757.3 |
CG2714-RA, transcript variant A (crm), mRNA |
| 0 |
0 |
6 |
2 |
5 |
NM_143349.1 |
CG4869-RA (betaTub97EF), mRNA |
| 0 |
0 |
4 |
9 |
2 |
NM_176161.1 |
CG33007-RA (CG33007), mRNA |
| 0 |
0 |
3 |
6 |
7 |
NM_080271.2 |
CG4625-RA (Dhap-at), mRNA |
| 0 |
0 |
3 |
2 |
4 |
NM_135755.1 |
CG9932-RA (CG9932), mRNA |
| 0 |
0 |
2 |
6 |
21 |
NM_135239.1 |
CG18304-RA (CG18304), mRNA |
| 0 |
0 |
2 |
5 |
4 |
NM_165064.1 |
CG7885-RB, transcript variant B (RpII33), mRNA |
| 0 |
0 |
2 |
5 |
4 |
NM_058071.3 |
CG7885-RA, transcript variant A (RpII33), mRNA |
| 0 |
0 |
2 |
4 |
14 |
NM_001043082.1 |
CG11798-RC, transcript variant C (chn), mRNA |
| 0 |
0 |
2 |
4 |
14 |
NM_206119.1 |
CG11798-RB, transcript variant B (chn), mRNA |
| 0 |
0 |
2 |
4 |
14 |
NM_137169.3 |
CG11798-RA, transcript variant A (chn), mRNA |
| 0 |
0 |
2 |
4 |
7 |
NM_167401.1 |
CG1517-RB, transcript variant B (na), mRNA |
| 0 |
0 |
2 |
4 |
7 |
NM_167402.1 |
CG1517-RC, transcript variant C (na), mRNA |
| 0 |
0 |
2 |
4 |
5 |
NM_001043081.1 |
CG11798-RD, transcript variant D (chn), mRNA |
| 0 |
0 |
2 |
3 |
5 |
NM_057309.2 |
CG6534-RA (slou), mRNA |
| 0 |
0 |
2 |
3 |
5 |
NM_166223.1 |
CG6556-RA (cnk), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_140094.1 |
CG14174-RA (CG14174), mRNA |
| 0 |
0 |
2 |
2 |
8 |
NM_135156.2 |
CG13991-RA (CG13991), mRNA |
| 0 |
0 |
2 |
2 |
6 |
NM_132155.2 |
CG4617-RA (CG4617), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_140674.2 |
CG11915-RA (CG11915), mRNA |
| 0 |
0 |
1 |
7 |
13 |
NM_165181.1 |
CG17927-RC, transcript variant C (Mhc), mRNA |
| 0 |
0 |
1 |
7 |
13 |
NM_165188.1 |
CG17927-RI, transcript variant I (Mhc), mRNA |
| 0 |
0 |
1 |
7 |
13 |
NM_165186.1 |
CG17927-RD, transcript variant D (Mhc), mRNA |
| 0 |
0 |
1 |
7 |
11 |
NM_165189.1 |
CG17927-RB, transcript variant B (Mhc), mRNA |
| 0 |
0 |
1 |
7 |
11 |
NM_165192.1 |
CG17927-RM, transcript variant M (Mhc), mRNA |
| 0 |
0 |
1 |
7 |
11 |
NM_165191.1 |
CG17927-RL, transcript variant L (Mhc), mRNA |
|
| Wing Disc |
GFP
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Puc
 |
Caspase
 |
DAPI
 |
Merge
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Figure
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|
JNK signal
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Caspase-3 signal
| N |
 |
| ++ |
|
| DB |
 |
| + |
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| DC |
 |
| ++ |
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| DM |
 |
| ++ |
|
| VM |
 |
| + |
|
| VB |
 |
| |
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