|
Notice:
|
|
Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
|
|
| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
discarded
|
| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
10909R-1 |
| Symbol |
CG10909 |
Full Name |
CG10909 |
| CG No |
CG10909 |
Old CG No |
CG10909 |
| Synonyms |
CG10909 |
| Accession No (Link to NCBI) |
NM_141979.1 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgCCCTCCAAGCAGTACTCTTGA |
| Primer Seq. 3' |
aatctagaggtaccCCATTATTCCAGCTGCCAGTT |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 CCCTCCAAGC AGTACTCTTG AAGAAGAAGT TCCTGGAAAG ACCATCCACC CTGAGGATCC
0061 GAAAATTACA TCTAAAAACT TCGAGAATCC CACAAAAGAA ACTTCCATCC AGAGCCCCAT
0121 TAAGAACTCC ATAAAACCAT CGTGGAATCC AGAACCCAAG ATCACTAGGA CCTCCATATG
0181 GGACATTGAA TCCGCAGGAG GGGATTCAAT ACAAAATGAA TTGTCAAAGT GAATGTAACG
0241 TTGAAGATCA AGGTCAAGAG CAAGAGGTCA CGTTGGAGAC TAGGATATTT TCTGGAACCT
0301 CAGACCGCAT TGAACCGCAC CGACATTACG GCGTCTATTT GTTGCGCAAC CGCTTCGATG
0361 CCATACAATT GCTCACCAGG AATACGAGCT CCAGTGCGGA TGACTACGGT GAGCGGAGGG
0421 TCATTTCGGA ATACCGGGAA ATGCGATGTG AATTCCGAGT CTGGTCTCCC TTCCAATCCA
0481 AACTGGCAGC TGGAATAATG G |
| in silico PCR Fragment |
0001 CCCTCCAAGC AGTACTCTTG AAGAAGAAGT TCCTGGAAAG ACCATCCACC CTGAGGATCC
0061 GAAAATTACA TCTAAAAACT TCGAGAATCC CACAAAAGAA ACTTCCATCC AGAGCCCCAT
0121 TAAGAACTCC ATAAAACCAT CGTGGAATCC AGAACCCAAG ATCACTAGGA CCTCCATATG
0181 GGACATTGAA TCCGCAGGAG GGGATTCAAT ACAAAATG-A TTGTCAAAGT GAATGTAACG
0241 TTGAAGATCA AGGTCATGAG CAAGAGGTCA CGTTGGAGAC TAGGATATTT TCTGGAACCT
0301 CAGACCGCAT TGAACCGCAC CGACATTACG GCGTCTATTT GTTGCGCAAC CGCTTCGATG
0361 CCATACAATT GCTCACCAGG AATACGAGCT CCAGTGCGGA TGACTACGGT GAGCGGAGGG
0421 TCATTTCGGA ATACCGGGAA ATGCGATGTG AATTCCGAGT CTGGTCTCCC TTCCAATCCA
0481 AACTGGCAGC TGGAATAATG G |
| Assemble Data |
10909R-1.IR_full 1 CCCTCCAAGCAGTACTCTTGAAGAAGAAGTTCCTGGAAAGACCATCCACCCTGAGGATCC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10909R-1.in silico 1 CCCTCCAAGCAGTACTCTTGAAGAAGAAGTTCCTGGAAAGACCATCCACCCTGAGGATCC 60
10909R-1.IR_full 61 GAAAATTACATCTAAAAACTTCGAGAATCCCACAAAAGAAACTTCCATCCAGAGCCCCAT 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10909R-1.in silico 61 GAAAATTACATCTAAAAACTTCGAGAATCCCACAAAAGAAACTTCCATCCAGAGCCCCAT 120
10909R-1.IR_full 121 TAAGAACTCCATAAAACCATCGTGGAATCCAGAACCCAAGATCACTAGGACCTCCATATG 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10909R-1.in silico 121 TAAGAACTCCATAAAACCATCGTGGAATCCAGAACCCAAGATCACTAGGACCTCCATATG 180
10909R-1.IR_full 181 GGACATTGAATCCGCAGGAGGGGATTCAATACAAAATGAATTGTCAAAGTGAATGTAACG 240
|||||||||||||||||||||||||||||||||||||| |||||||||||||||||||||
10909R-1.in silico 181 GGACATTGAATCCGCAGGAGGGGATTCAATACAAAATG-ATTGTCAAAGTGAATGTAACG 240
10909R-1.IR_full 241 TTGAAGATCAAGGTCAAGAGCAAGAGGTCACGTTGGAGACTAGGATATTTTCTGGAACCT 300
|||||||||||||||| |||||||||||||||||||||||||||||||||||||||||||
10909R-1.in silico 241 TTGAAGATCAAGGTCATGAGCAAGAGGTCACGTTGGAGACTAGGATATTTTCTGGAACCT 300
10909R-1.IR_full 301 CAGACCGCATTGAACCGCACCGACATTACGGCGTCTATTTGTTGCGCAACCGCTTCGATG 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10909R-1.in silico 301 CAGACCGCATTGAACCGCACCGACATTACGGCGTCTATTTGTTGCGCAACCGCTTCGATG 360
10909R-1.IR_full 361 CCATACAATTGCTCACCAGGAATACGAGCTCCAGTGCGGATGACTACGGTGAGCGGAGGG 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10909R-1.in silico 361 CCATACAATTGCTCACCAGGAATACGAGCTCCAGTGCGGATGACTACGGTGAGCGGAGGG 420
10909R-1.IR_full 421 TCATTTCGGAATACCGGGAAATGCGATGTGAATTCCGAGTCTGGTCTCCCTTCCAATCCA 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10909R-1.in silico 421 TCATTTCGGAATACCGGGAAATGCGATGTGAATTCCGAGTCTGGTCTCCCTTCCAATCCA 480
10909R-1.IR_full 481 AACTGGCAGCTGGAATAATGG 501
|||||||||||||||||||||
10909R-1.in silico 481 AACTGGCAGCTGGAATAATGG 501
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_141979.1 |
CG10909-RA (CG10909), mRNA |
| 0 |
0 |
2 |
4 |
2 |
NM_143656.1 |
CG1674-RB, transcript variant B (CG1674), mRNA |
| 0 |
0 |
2 |
4 |
2 |
NM_166746.1 |
CG1674-RC, transcript variant C (CG1674), mRNA |
| 0 |
0 |
2 |
4 |
2 |
NM_166747.1 |
CG1674-RA, transcript variant A (CG1674), mRNA |
| 0 |
0 |
2 |
4 |
2 |
NM_205866.1 |
CG1674-RD, transcript variant D (CG1674), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_057599.3 |
CG3738-RA (Cks30A), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_176767.1 |
CG1668-RB, transcript variant B (Pbprp2), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_078697.2 |
CG1668-RA, transcript variant A (Pbprp2), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_139425.1 |
CG5691-RA (CG5691), mRNA |
| 0 |
0 |
0 |
6 |
3 |
NM_133121.3 |
CG7440-RA, transcript variant A (tgy), mRNA |
| 0 |
0 |
0 |
6 |
3 |
NM_001038765.1 |
CG7440-RB, transcript variant B (tgy), mRNA |
| 0 |
0 |
0 |
5 |
3 |
NM_141316.1 |
CG1347-RA, transcript variant A (CG1347), mRNA |
| 0 |
0 |
0 |
5 |
3 |
NM_206434.1 |
CG1347-RB, transcript variant B (CG1347), mRNA |
| 0 |
0 |
0 |
5 |
2 |
NM_141601.2 |
CG11981-RA (Prosbeta3), mRNA |
| 0 |
0 |
0 |
4 |
6 |
NM_164899.1 |
CG5686-RA (chico), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_079624.3 |
CG14360-RA (Or88a), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_134676.1 |
CG4213-RA (CG4213), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_139511.2 |
CG1869-RA (CG1869), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_078670.2 |
CG7092-RA (Dhc16F), mRNA |
| 0 |
0 |
0 |
3 |
7 |
NM_136747.2 |
CG11761-RA (translin), mRNA |
| 0 |
0 |
0 |
3 |
4 |
NM_001014696.1 |
CG18069-RG, transcript variant G (CaMKII), mRNA |
| 0 |
0 |
0 |
3 |
4 |
NM_166813.2 |
CG18069-RE, transcript variant E (CaMKII), mRNA |
| 0 |
0 |
0 |
3 |
4 |
NM_166812.2 |
CG18069-RD, transcript variant D (CaMKII), mRNA |
| 0 |
0 |
0 |
3 |
4 |
NM_079896.3 |
CG18069-RC, transcript variant C (CaMKII), mRNA |
| 0 |
0 |
0 |
3 |
4 |
NM_166810.3 |
CG18069-RA, transcript variant A (CaMKII), mRNA |
| 0 |
0 |
0 |
3 |
4 |
NM_166811.1 |
CG18069-RB, transcript variant B (CaMKII), mRNA |
| 0 |
0 |
0 |
3 |
4 |
NM_141254.1 |
CG2022-RA (CG2022), mRNA |
| 0 |
0 |
0 |
3 |
3 |
NM_168101.1 |
CG32236-RA (CG32236), mRNA |
| 0 |
0 |
0 |
3 |
3 |
NM_136805.1 |
CG13215-RA (CG13215), mRNA |
| 0 |
0 |
0 |
3 |
2 |
NM_001031974.1 |
CG33771-RA (CG33771), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
 |
|
JNK signal
|
Caspase-3 signal
| N |
 |
| ++ |
|
| DB |
 |
| + |
|
| DC |
 |
| ++ |
|
| DM |
 |
| + |
|
| VM |
 |
| + |
|
| VB |
 |
| |
|
|
|
|
|
|
