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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 10898R-3 
 Symbol CG10898  Full Name CG10898 
 CG No CG10898  Old CG No CG10898 
 Synonyms anon-WO0118547.280, CG10898 
 Accession No (Link to NCBI) NM_141826.2 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgGATTGTGGAGAAAGTGCGTCG 
 Primer Seq. 3' 
aatctagaggtaccCTCGGAGAGGTACTTCCTTGG 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 NATTGTGGAG AAAGTGCGTC gNATTTTGGA CTCAAAAGAT CTGGGCGACA TCACAACGGA 
0061 ACTGTGTGAC TTTTCTCTTA AGGAACAGAA TGCCACCGCC GAGGCCCAGG GAGTACAGCC 
0121 CTCATCCGCA TCCGATTTTG TGCCCATTCT TGGACAGACA GTCACCTATA TTGTGGCCTG 
0181 TGTGTTGATC AATGAGCACG ACGAGCTTCT GATGATCGAG GAGGCCAAAC AGAGCTGTGC 
0241 CGGCAAGTGG TACTTACCCG CTGGTCGCAT GGAAAGGGGC GAATCCATCA CGGANGCAGC 
0301 CGCCCGNGGA AGTTTTCGAA GAGACTGGTC TAAATGCCGA GCTGACCACT CTATTAGCGG 
0361 TGGAGNCNGC CGGTGGCTCT TGGTTCCGCT TCGTGCTCAC TGGACGCATC ACAGGCGGCC 
0421 GACTTAAAAC TcCNGCGGAT GCAGACGCCG AATCGATTCA GGCGAGATGG GTGCGTAATC 
0481 CCAAGGAAGT aCCTCTCCGA G 
 in silico PCR Fragment 
0001 GATTGTGGAG AAAGTGCGTC GCATTTTGGA CTCAAAAGAT CTGGGCGACA TCACAACGGA 
0061 ACTGTGTGAC TTTTCTCTTA AGGAACAGAA TGCCACCGCC GAGGCCCAGG GAGTACAGCC 
0121 CTCATCCGCA TCCGATTTTG TGCCCATTCT TGGACAGACA GTCACCTATA TTGTGGCCTG 
0181 TGTGTTGATC AATGAGCACG ACGAGCTTCT GATGATCGAG GAGGCCAAAC AGAGCTGTGC 
0241 CGGCAAGTGG TACTTACCCG CTGGTCGCAT GGAAAGGGGC GAATCCATCA CGGAGGCAGC 
0301 CGCCCG-GGA AGTTTTCGAA GAGACTGGTC TAAATGCCGA GCTGACCACT CTATTAGCGG 
0361 TGGAGGCCGC CGGTGGCTCT TGGTTCCGCT TCGTGCTCAC TGGACGCATC ACAGGCGGCC 
0421 GACTTAAAAC TCCTGCGGAT GCAGACGCCG AATCGATTCA GGCGAGATGG GTGCGTAATC 
0481 CCAAGGAAGT ACCTCTCCGA G 
 Assemble Data 

10898R-3.IR_full        1   ATTGTGGAGAAAGTGCGTCGNATTTTGGACTCAAAAGATCTGGGCGACATCACAACGGA 59
                            |||||||||||||||||||| ||||||||||||||||||||||||||||||||||||||
10898R-3.in silico      1   ATTGTGGAGAAAGTGCGTCGCATTTTGGACTCAAAAGATCTGGGCGACATCACAACGGA 59


10898R-3.IR_full       61  ACTGTGTGACTTTTCTCTTAAGGAACAGAATGCCACCGCCGAGGCCCAGGGAGTACAGCC 119
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10898R-3.in silico     61  ACTGTGTGACTTTTCTCTTAAGGAACAGAATGCCACCGCCGAGGCCCAGGGAGTACAGCC 119


10898R-3.IR_full       121 CTCATCCGCATCCGATTTTGTGCCCATTCTTGGACAGACAGTCACCTATATTGTGGCCTG 179
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10898R-3.in silico     121 CTCATCCGCATCCGATTTTGTGCCCATTCTTGGACAGACAGTCACCTATATTGTGGCCTG 179


10898R-3.IR_full       181 TGTGTTGATCAATGAGCACGACGAGCTTCTGATGATCGAGGAGGCCAAACAGAGCTGTGC 239
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10898R-3.in silico     181 TGTGTTGATCAATGAGCACGACGAGCTTCTGATGATCGAGGAGGCCAAACAGAGCTGTGC 239


10898R-3.IR_full       241 CGGCAAGTGGTACTTACCCGCTGGTCGCATGGAAAGGGGCGAATCCATCACGGANGCAGC 299
                           |||||||||||||||||||||||||||||||||||||||||||||||||||||| |||||
10898R-3.in silico     241 CGGCAAGTGGTACTTACCCGCTGGTCGCATGGAAAGGGGCGAATCCATCACGGAGGCAGC 299


10898R-3.IR_full       301 CGCCCGNGGAAGTTTTCGAAGAGACTGGTCTAAATGCCGAGCTGACCACTCTATTAGCGG 359
                           |||||| |||||||||||||||||||||||||||||||||||||||||||||||||||||
10898R-3.in silico     301 CGCCCG-GGAAGTTTTCGAAGAGACTGGTCTAAATGCCGAGCTGACCACTCTATTAGCGG 359


10898R-3.IR_full       361 TGGAGNCNGCCGGTGGCTCTTGGTTCCGCTTCGTGCTCACTGGACGCATCACAGGCGGCC 419
                           ||||| | ||||||||||||||||||||||||||||||||||||||||||||||||||||
10898R-3.in silico     361 TGGAGGCCGCCGGTGGCTCTTGGTTCCGCTTCGTGCTCACTGGACGCATCACAGGCGGCC 419


10898R-3.IR_full       421 GACTTAAAACTCCNGCGGATGCAGACGCCGAATCGATTCAGGCGAGATGGGTGCGTAATC 479
                           ||||||||||||| ||||||||||||||||||||||||||||||||||||||||||||||
10898R-3.in silico     421 GACTTAAAACTCCTGCGGATGCAGACGCCGAATCGATTCAGGCGAGATGGGTGCGTAATC 479


10898R-3.IR_full       481 CCAAGGAAGTACCTCTCCGAG 500
                           |||||||||||||||||||||
10898R-3.in silico     481 CCAAGGAAGTACCTCTCCGAG 500



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  2  NM_141826.2  CG10898-RA (CG10898), mRNA 
0   0  4  6  6  NM_137517.4  CG18604-RA (CG18604), mRNA 
0   0  3  2  5  NM_170398.1  CG14516-RA, transcript variant A (CG14516), mRNA 
0   0  3  2  5  NM_143417.2  CG14516-RB, transcript variant B (CG14516), mRNA 
0   0  2  4  13  NM_167283.1  CG32664-RA (Gr10a), mRNA 
0   0  2  2  4  NM_142586.1  CG4538-RA, transcript variant A (CG4538), mRNA 
0   0  2  2  4  NM_169880.1  CG4538-RB, transcript variant B (CG4538), mRNA 
0   0  2  2  3  NM_166186.2  CG18471-RA (gprs), mRNA 
0   0  1  3  5  NM_137791.2  CG2921-RA (CG2921), mRNA 
0   0  1  2  7  NM_132302.3  CG7055-RA (dalao), mRNA 
0   0  1  2  4  NM_170213.2  CG31102-RA (CG31102), mRNA 
0   0  1  2  3  NM_057679.4  CG5371-RA (RnrL), mRNA 
0   0  1  2  2  NM_167940.1  CG32305-RA (CG32305), mRNA 
0   0  0  6  7  NM_140377.1  CG17687-RA (CG17687), mRNA 
0   0  0  5  6  NM_142746.1  CG6022-RA (CG6022), mRNA 
0   0  0  4  7  NM_058135.2  CG10917-RA (fj), mRNA 
0   0  0  4  5  NM_143141.2  CG4548-RB, transcript variant B (XNP), mRNA 
0   0  0  4  5  NM_170228.1  CG4548-RA, transcript variant A (XNP), mRNA 
0   0  0  4  5  NM_141936.1  CG6525-RA (CG6525), mRNA 
0   0  0  4  3  NM_132014.2  CG4119-RA (CG4119), mRNA 
0   0  0  4  3  NM_001042891.1  CG31761-RE, transcript variant E (bru-2), mRNA 
0   0  0  4  2  NM_132016.1  CG15778-RA (CG15778), mRNA 
0   0  0  4  2  NM_078532.1  CG1478-RA (Cp36), mRNA 
0   0  0  3  11  NM_206430.1  CG2666-RC, transcript variant C (kkv), mRNA 
0   0  0  3  11  NM_079509.1  CG2666-RA, transcript variant A (kkv), mRNA 
0   0  0  3  11  NM_169052.1  CG2666-RB, transcript variant B (kkv), mRNA 
0   0  0  3  7  NM_167357.1  CG15745-RB, transcript variant B (CG15745), mRNA 
0   0  0  3  7  NM_132645.2  CG15745-RA, transcript variant A (CG15745), mRNA 
0   0  0  3  6  NM_141341.2  CG1208-RA (CG1208), mRNA 
0   0  0  3  6  NM_142866.1  CG6733-RA (CG6733), mRNA 
 Wing Disc
GFP
 Puc
 Caspase
DAPI
 Merge
 Figure
JNK signal
N
 
DB
+- 
DC
++ 
DM
 
VM
 
VB
 
Caspase-3 signal
N
+ 
DB
+ 
DC
+ 
DM
+ 
VM
+ 
VB
 
Dcompartment   Penetrance
     

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