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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
10888R-1 |
| Symbol |
Rh3 |
Full Name |
Rhodopsin 3 |
| CG No |
CG10888 |
Old CG No |
CG10888 |
| Synonyms |
rh3, Rh, FBgn0003249, DMELRH3, Dm Rh3, RH3, unnamed, CG10888, Rh3 |
| Accession No (Link to NCBI) |
NM_079687.2 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Lesch C, Jo J, Wu Y, Fish GS, Galko MJ.
A targeted UAS-RNAi screen in Drosophila larvae identifies wound closure genes regulating distinct cellular processes.
Genetics (2010) 186(3) 943-57
[
PubMed ID = 20813879
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgATGGAGTCCGGTAACGTGTCG |
| Primer Seq. 3' |
aatctagaggtaccATCTTGCCCTCCATGGGTCGA |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 ATGGAGTCCG GTAACGTGTC GTCGAGTCTG TTTGGCAACG TGTNCCACCG CGCTGCgGCC
0061 GGAGNCGCTG GCTCTCCGCC GAAACGCGTC TGCTGGGCTG GAATNTGCCG CCGGAGGAGC
0121 TGCGGCACAT TCCCGAGCAC TGGCTAACTT ACCCGGAGCC GCCCGAATCG ATGAACTACC
0181 TGCTGGGCAC GCTCTACATC TTCTTCACCC TGATGTCGAT GCTGGGCAAT GGACTGGTGA
0241 TTTGGGTCTT NCTCCGCAGC CAAATCGCTG CGAACTCCCT CCAATATACT GGTCATCAAT
0301 CTGGCCTTCT GCGACTTCAT GATGATGGTC AAGACTCCGA TATTCATCTA CAATAGCTTC
0361 CACCAGGGAT ATGCGCTGGG TCATCTGGGA TGCCAGATCT TTGGAATCAT TGGCTNCTAT
0421 ACGGGAATCG CTGCCGGTGC CACCAATGCG TTTATAGCCT ACGATCGATT CAATGTGATC
0481 ACTCGACCCA TGGAGGGCAA GAT |
| in silico PCR Fragment |
0001 ATGGAGTCCG GTAACGTGTC GTCGAGTCTG TTTGGCAACG TGT-CCACCG CGCTGCGGCC
0061 GGAGGCGC-G GCTCTCCGCC GAAACGCGTC TGCTGGGCTG GAATGTGCCG CCGGAGGAGC
0121 TGCGGCACAT TCCCGAGCAC TGGCTAACTT ACCCGGAGCC GCCCGAATCG ATGAACTACC
0181 TGCTGGGCAC GCTCTACATC TTCTTCACCC TGATGTCGAT GCTGGGCAAT GGACTGGTGA
0241 TTTGGGTCTT -CTCCGCAGC CAAATCGCTG CGAACTCCCT CCAATATACT GGTCATCAAT
0301 CTGGCCTTCT GCGACTTCAT GATGATGGTC AAGACTCCGA TATTCATCTA CAATAGCTTC
0361 CACCAGGGAT ATGCGCTGGG TCATCTGGGA TGCCAGATCT TTGGAATCAT TGGCTCCTAT
0421 ACGGGAATCG CTGCCGGTGC CACCAATGCG TTTATAGCCT ACGATCGATT CAATGTGATC
0481 ACTCGACCCA TGGAGGGCAA GAT |
| Assemble Data |
10888R-1.IR_full 1 ATGGAGTCCGGTAACGTGTCGTCGAGTCTGTTTGGCAACGTGTNCCACCGCGCTGCGGCC 60
||||||||||||||||||||||||||||||||||||||||||| ||||||||||||||||
10888R-1.in silico 1 ATGGAGTCCGGTAACGTGTCGTCGAGTCTGTTTGGCAACGTGT-CCACCGCGCTGCGGCC 60
10888R-1.IR_full 61 GGAGNCGCTGGCTCTCCGCCGAAACGCGTCTGCTGGGCTGGAATNTGCCGCCGGAGGAGC 120
|||| ||| ||||||||||||||||||||||||||||||||||| |||||||||||||||
10888R-1.in silico 61 GGAGGCGC-GGCTCTCCGCCGAAACGCGTCTGCTGGGCTGGAATGTGCCGCCGGAGGAGC 120
10888R-1.IR_full 121 TGCGGCACATTCCCGAGCACTGGCTAACTTACCCGGAGCCGCCCGAATCGATGAACTACC 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10888R-1.in silico 121 TGCGGCACATTCCCGAGCACTGGCTAACTTACCCGGAGCCGCCCGAATCGATGAACTACC 180
10888R-1.IR_full 181 TGCTGGGCACGCTCTACATCTTCTTCACCCTGATGTCGATGCTGGGCAATGGACTGGTGA 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10888R-1.in silico 181 TGCTGGGCACGCTCTACATCTTCTTCACCCTGATGTCGATGCTGGGCAATGGACTGGTGA 240
10888R-1.IR_full 241 TTTGGGTCTTNCTCCGCAGCCAAATCGCTGCGAACTCCCTCCAATATACTGGTCATCAAT 300
|||||||||| |||||||||||||||||||||||||||||||||||||||||||||||||
10888R-1.in silico 241 TTTGGGTCTT-CTCCGCAGCCAAATCGCTGCGAACTCCCTCCAATATACTGGTCATCAAT 300
10888R-1.IR_full 301 CTGGCCTTCTGCGACTTCATGATGATGGTCAAGACTCCGATATTCATCTACAATAGCTTC 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10888R-1.in silico 301 CTGGCCTTCTGCGACTTCATGATGATGGTCAAGACTCCGATATTCATCTACAATAGCTTC 360
10888R-1.IR_full 361 CACCAGGGATATGCGCTGGGTCATCTGGGATGCCAGATCTTTGGAATCATTGGCTNCTAT 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||| ||||
10888R-1.in silico 361 CACCAGGGATATGCGCTGGGTCATCTGGGATGCCAGATCTTTGGAATCATTGGCTCCTAT 420
10888R-1.IR_full 421 ACGGGAATCGCTGCCGGTGCCACCAATGCGTTTATAGCCTACGATCGATTCAATGTGATC 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10888R-1.in silico 421 ACGGGAATCGCTGCCGGTGCCACCAATGCGTTTATAGCCTACGATCGATTCAATGTGATC 480
10888R-1.IR_full 481 ACTCGACCCATGGAGGGCAAGAT 503
|||||||||||||||||||||||
10888R-1.in silico 481 ACTCGACCCATGGAGGGCAAGAT 503
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_079687.2 |
CG10888-RA (Rh3), mRNA |
| 0 |
0 |
16 |
5 |
6 |
NM_079644.2 |
CG5192-RB (Rh6), mRNA |
| 0 |
0 |
10 |
26 |
28 |
NM_057353.3 |
CG9668-RA (Rh4), mRNA |
| 0 |
0 |
2 |
5 |
2 |
NM_167001.1 |
CG4944-RA, transcript variant A (cib), mRNA |
| 0 |
0 |
2 |
5 |
2 |
NM_167002.1 |
CG4944-RC, transcript variant C (cib), mRNA |
| 0 |
0 |
2 |
5 |
2 |
NM_080326.4 |
CG4944-RB, transcript variant B (cib), mRNA |
| 0 |
0 |
2 |
4 |
28 |
NM_079311.2 |
CG5638-RA (Rh7), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_135935.2 |
CG12455-RA, transcript variant A (CG12455), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_165149.1 |
CG12455-RB, transcript variant B (CG12455), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_135572.2 |
CG6094-RA (CG6094), mRNA |
| 0 |
0 |
1 |
6 |
5 |
NM_170430.2 |
CG31037-RA (ca), mRNA |
| 0 |
0 |
1 |
6 |
5 |
NM_141777.2 |
CG6608-RA, transcript variant A (CG6608), mRNA |
| 0 |
0 |
1 |
6 |
5 |
NM_169372.1 |
CG6608-RB, transcript variant B (CG6608), mRNA |
| 0 |
0 |
1 |
6 |
2 |
NM_057800.3 |
CG2913-RB, transcript variant B (yin), mRNA |
| 0 |
0 |
1 |
6 |
2 |
NM_166993.1 |
CG2913-RC, transcript variant C (yin), mRNA |
| 0 |
0 |
1 |
6 |
2 |
NM_057799.3 |
CG2913-RA, transcript variant A (yin), mRNA |
| 0 |
0 |
1 |
5 |
8 |
NM_137925.2 |
CG3162-RA (CG3162), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_137701.2 |
CG4030-RA (CG4030), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_132073.1 |
CG14445-RA (CG14445), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_057661.2 |
CG5076-RA (elk), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_139494.1 |
CG14952-RA (CG14952), mRNA |
| 0 |
0 |
1 |
2 |
9 |
NM_078559.2 |
CG18085-RA (sev), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_164339.3 |
CG9776-RB, transcript variant B (CG9776), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_141198.3 |
CG9776-RA, transcript variant A (CG9776), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_132679.1 |
CG12176-RA (Lig4), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_079797.2 |
CG6348-RA, transcript variant A (ro), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_170294.1 |
CG6348-RB, transcript variant B (ro), mRNA |
| 0 |
0 |
0 |
6 |
10 |
NM_079683.2 |
CG4550-RA (ninaE), mRNA |
| 0 |
0 |
0 |
6 |
9 |
NM_079674.2 |
CG16740-RA (Rh2), mRNA |
| 0 |
0 |
0 |
6 |
4 |
NM_132010.1 |
CG17758-RA (CG17758), mRNA |
|
| Wing Disc |
GFP
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Puc
 |
Caspase
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DAPI
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Merge
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Figure
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JNK signal
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Caspase-3 signal
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