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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 10883R-2 
 Symbol CG10883  Full Name CG10883 
 CG No CG10883  Old CG No CG10883 
 Synonyms CG10883 
 Accession No (Link to NCBI) NM_142617.1 
 Inserted Chr. ll 
 Insertional Mutation  lethal 
 Phenotype induced by Act5C-GAL4 at 28 degrees pupal lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgATGATTGATTGTGGCTGCGTG 
 Primer Seq. 3' 
aatctagaggtaccAACAACCGCAACTGCAGCAAC 
 Predicted Fragment Size 
424 
 IR fragment full Seq 
0001 NTGATTGATT GTGGCTGCGT GACTATTGTT GCCGCTGNCA ACATGTTGCT TGTAAGTACT 
0061 ACCGCTGGCA TGTTGCGGGG ATGCAATGAT AATACGTTGC TGCTGCTGCT GCTTGATATC 
0121 GCTGCTGCTG TGACTGCTGT TGTTGCTGCT GCTGCTGTTG CTACTGTTGT TGCCGGCGCC 
0181 TGCACCGCAA CTCACGATCA TGCGATGCTG CTGATTGCCG TTgGCCAGGC CATCGACGTT 
0241 CCCAGTTGTT TCGGCGGGTT TCTGCTGCTT CTGCAGACTC ACTtgcTGCT GCTGCTGCTG 
0301 CTGGGGATGC TGCTCGGTCT TGTCCAGCTC GGCTGGATCG AACTTTCGCT TCTTGGGAtG 
0361 CTGCCGCGGC TGAGGTGGTT GCTGTTGCTG AGGTGTGCTG CAGGTTGCTG CAGTTGCGGT 
0421 TGTT 
 in silico PCR Fragment 
0001 ATGATTGATT GTGGCTGCGT GACTATTGTT GCCGCTGGCA ACATGTTGCT TGTAAGTACT 
0061 ACCGCTGGCA TGTTGCGGGG ATGCAATGAT AATACGTTGC TGCTGCTGCT GCTTGATATC 
0121 GCTGCTGCTG TGACTGCTGT TGTTGCTGCT GCTGCTGTTG CTACTGTTGT TGCCGGCGCC 
0181 TGCACCGCAA CTCACGATCA TGCGATGCTG CTGATTGCCG TTGGCCAGGC CATCGCCGTT 
0241 CCCAGTTGTT TCGGCGGGTT TCTGCTGCTT CTGCAGACTC ACTTGCTGCT GCTGCTGCTG 
0301 CTGGGGATGC TGCTCGGTCT TGTCCAGCTC GGCTGGATCG AACTTTCGCT TCTTGGGATG 
0361 CTGCCGCGGC TGAGGTGGTT GCTGTTGCTG AGGTGTGCTG CAGGTTGCTG CAGTTGCGGT 
0421 TGTT 
 Assemble Data 

10883R-2.IR_full        1   TGATTGATTGTGGCTGCGTGACTATTGTTGCCGCTGNCAACATGTTGCTTGTAAGTACT 59
                            |||||||||||||||||||||||||||||||||||| ||||||||||||||||||||||
10883R-2.in silico      1   TGATTGATTGTGGCTGCGTGACTATTGTTGCCGCTGGCAACATGTTGCTTGTAAGTACT 59


10883R-2.IR_full       61  ACCGCTGGCATGTTGCGGGGATGCAATGATAATACGTTGCTGCTGCTGCTGCTTGATATC 119
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10883R-2.in silico     61  ACCGCTGGCATGTTGCGGGGATGCAATGATAATACGTTGCTGCTGCTGCTGCTTGATATC 119


10883R-2.IR_full       121 GCTGCTGCTGTGACTGCTGTTGTTGCTGCTGCTGCTGTTGCTACTGTTGTTGCCGGCGCC 179
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10883R-2.in silico     121 GCTGCTGCTGTGACTGCTGTTGTTGCTGCTGCTGCTGTTGCTACTGTTGTTGCCGGCGCC 179


10883R-2.IR_full       181 TGCACCGCAACTCACGATCATGCGATGCTGCTGATTGCCGTTGGCCAGGCCATCGACGTT 239
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||| ||||
10883R-2.in silico     181 TGCACCGCAACTCACGATCATGCGATGCTGCTGATTGCCGTTGGCCAGGCCATCGCCGTT 239


10883R-2.IR_full       241 CCCAGTTGTTTCGGCGGGTTTCTGCTGCTTCTGCAGACTCACTTGCTGCTGCTGCTGCTG 299
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10883R-2.in silico     241 CCCAGTTGTTTCGGCGGGTTTCTGCTGCTTCTGCAGACTCACTTGCTGCTGCTGCTGCTG 299


10883R-2.IR_full       301 CTGGGGATGCTGCTCGGTCTTGTCCAGCTCGGCTGGATCGAACTTTCGCTTCTTGGGATG 359
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10883R-2.in silico     301 CTGGGGATGCTGCTCGGTCTTGTCCAGCTCGGCTGGATCGAACTTTCGCTTCTTGGGATG 359


10883R-2.IR_full       361 CTGCCGCGGCTGAGGTGGTTGCTGTTGCTGAGGTGTGCTGCAGGTTGCTGCAGTTGCGGT 419
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10883R-2.in silico     361 CTGCCGCGGCTGAGGTGGTTGCTGTTGCTGAGGTGTGCTGCAGGTTGCTGCAGTTGCGGT 419


10883R-2.IR_full       421 TGTT 423
                           ||||
10883R-2.in silico     421 TGTT 423



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
101.23  411  7  100  209  NM_142617.1  CG10883-RA (CG10883), mRNA 
34.97   142  605  1800  3598  NM_080536.2  CG4013-RA, transcript variant A (Smr), mRNA 
34.97   142  605  1800  3598  NM_167334.1  CG4013-RB, transcript variant B (Smr), mRNA 
34.97   142  605  1800  3598  NM_167335.1  CG4013-RC, transcript variant C (Smr), mRNA 
11.08   45  209  745  1740  NM_080376.1  CG8118-RA, transcript variant A (mam), mRNA 
11.08   45  209  745  1740  NM_166030.1  CG8118-RB, transcript variant B (mam), mRNA 
9.85   40  115  304  801  NM_169258.1  CG9755-RA, transcript variant A (pum), mRNA 
9.85   40  115  304  801  NM_169259.1  CG9755-RD, transcript variant D (pum), mRNA 
9.85   40  115  304  801  NM_079561.2  CG9755-RC, transcript variant C (pum), mRNA 
9.11   37  103  364  941  NM_139799.3  CG10107-RA, transcript variant A (CG10107), mRNA 
9.11   37  103  364  941  NM_206286.1  CG10107-RC, transcript variant C (CG10107), mRNA 
8.37   34  56  269  532  NM_079939.2  CG5580-RA, transcript variant A (sbb), mRNA 
7.63   31  143  462  1174  NM_131975.3  CG32767-RA, transcript variant A (CG32767), mRNA 
7.63   31  143  462  1174  NM_206628.1  CG32767-RB, transcript variant B (CG32767), mRNA 
7.63   31  98  394  831  NM_132246.2  CG10555-RA (CG10555), mRNA 
7.38   30  150  499  1194  NM_079671.2  CG7847-RA, transcript variant A (sr), mRNA 
7.38   30  126  404  964  NM_169786.1  CG7847-RB, transcript variant B (sr), mRNA 
7.14   29  133  390  786  NM_134474.4  CG32532-RA (CG32532), mRNA 
7.14   29  87  308  652  NM_167239.2  CG32677-RA (CG32677), mRNA 
6.89   28  170  506  1378  NM_168571.2  CG32133-RA (CG32133), mRNA 
6.89   28  86  352  666  NM_001014565.1  CG10491-RB, transcript variant B (vn), mRNA 
6.89   28  86  352  664  NM_079218.2  CG10491-RA, transcript variant A (vn), mRNA 
6.65   27  194  537  1494  NM_001038734.1  CG16902-RC (Hr4), mRNA 
6.4   26  66  229  383  NM_133012.2  CG32560-RA (CG32560), mRNA 
5.66   23  108  351  827  NM_079845.2  CG7951-RA (sima), mRNA 
5.41   22  81  250  572  NM_168413.1  CG32062-RB, transcript variant B (CG32062), mRNA 
5.41   22  81  250  572  NM_168412.1  CG32062-RD, transcript variant D (CG32062), mRNA 
5.41   22  67  276  525  NM_166992.2  CG2904-RA (ec), mRNA 
5.17   21  158  495  1294  NM_135077.2  CG14023-RA (CG14023), mRNA 
5.17   21  103  354  753  NM_140402.2  CG17689-RA, transcript variant A (CG17689), mRNA 

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