NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 10873R-1

Symbol p53 Full Name p53

CG No CG33336 Old CG No CG10873

Synonyms Dmp53, dp53, DmP53, dmp53, prac, Dm-P53, CG10873, CG33336, CG31325, p53

Accession No (Link to NCBI) NM_206545.1

Inserted Chr. lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees viable

Map Viewer

Reference
[Please submit your publication] Nitta Y, Yamazaki D, Sugie A, Hiroi M, Tabata T.
DISCO Interacting Protein 2 regulates axonal bifurcation and guidance of Drosophila mushroom body neurons.
Dev Biol (2017) 421(2) 233-244 [ PubMed ID = 27908785 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgACTCCACGGAGGTCGATATCA

Primer Seq. 3'

aatctagaggtaccTACACTGTTGGGATTCTCGCT

Predicted Fragment Size

IR fragment full Seq

0001 ACTCCACGGA GGTCGATATC AAGGAGGATA TTCCGAAAAC GGTGGAGGTA TCGGGATCGG 
0061 AATTGACCAC GGAACCCATG GCCTTCTTGC AGGGATTAAA CTCCGGGAAT CTGATGCAGT 
0121 TCAGCCAGCA ATCCGTGCTG CGCGAAATGA TGCTGCAGGA CATTCAGATC CAGGCGAACA 
0181 CGCTGCCCAA GCTAGAGAAT CACAACATCG GTGGTTATTG CTTCAGCATG GTTCTGGATG 
0241 AGCCGCCCAA GTCTCTTTGG ATGTACTCGA TTCCGCTGAA CAAGCTCTAC ATCCGGATGA 
0301 ACAAGGCCTT CAACGTGGAC GTTCAGTTCA AGTCTAAAAT GCCCATCCAA CCACTTAATT 
0361 TGCGTGTGTT CCTTTGCTTC TCCAATGATG TGAGTGCTCC CGTGGTCCGC TGTCAAAATC 
0421 ACCTTAGCGT TGAGNCTTTG ACGGCCAATA ACGCAAAAAT GCGCGAGAGC TTGCTGCGCA 
0481 GCGAGNAATC CCAACAGTGT A

in silico PCR Fragment

0001 ACTCCACGGA GGTCGATATC AAGGAGGATA TTCCGAAAAC GGTGGAGGTA TCGGGATCGG 
0061 AATTGACCAC GGAACCCATG GCCTTCTTGC AGGGATTAAA CTCCGGGAAT CTGATGCAGT 
0121 TCAGCCAGCA ATCCGTGCTG CGCGAAATGA TGCTGCAGGA CATTCAGATC CAGGCGAACA 
0181 CGCTGCCCAA GCTAGAGAAT CACAACATCG GTGGTTATTG CTTCAGCATG GTTCTGGATG 
0241 AGCCGCCCAA GTCTCTTTGG ATGTACTCGA TTCCGCTGAA CAAGCTCTAC ATCCGGATGA 
0301 ACAAGGCCTT CAACGTGGAC GTTCAGTTCA AGTCTAAAAT GCCCATCCAA CCACTTAATT 
0361 TGCGTGTGTT CCTTTGCTTC TCCAATGATG TGAGTGCTCC CGTGGTCCGC TGTCAAAATC 
0421 ACCTTAGCGT TGAGCCTTTG ACGGCCAATA ACGCAAAAAT GCGCGAGAGC TTGCTGCGCA 
0481 GCGAG-AATC CCAACAGTGT A

Assemble Data


10873R-1.IR_full       1   ACTCCACGGAGGTCGATATCAAGGAGGATATTCCGAAAACGGTGGAGGTATCGGGATCGG 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10873R-1.in silico     1   ACTCCACGGAGGTCGATATCAAGGAGGATATTCCGAAAACGGTGGAGGTATCGGGATCGG 60


10873R-1.IR_full       61  AATTGACCACGGAACCCATGGCCTTCTTGCAGGGATTAAACTCCGGGAATCTGATGCAGT 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10873R-1.in silico     61  AATTGACCACGGAACCCATGGCCTTCTTGCAGGGATTAAACTCCGGGAATCTGATGCAGT 120


10873R-1.IR_full       121 TCAGCCAGCAATCCGTGCTGCGCGAAATGATGCTGCAGGACATTCAGATCCAGGCGAACA 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10873R-1.in silico     121 TCAGCCAGCAATCCGTGCTGCGCGAAATGATGCTGCAGGACATTCAGATCCAGGCGAACA 180


10873R-1.IR_full       181 CGCTGCCCAAGCTAGAGAATCACAACATCGGTGGTTATTGCTTCAGCATGGTTCTGGATG 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10873R-1.in silico     181 CGCTGCCCAAGCTAGAGAATCACAACATCGGTGGTTATTGCTTCAGCATGGTTCTGGATG 240


10873R-1.IR_full       241 AGCCGCCCAAGTCTCTTTGGATGTACTCGATTCCGCTGAACAAGCTCTACATCCGGATGA 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10873R-1.in silico     241 AGCCGCCCAAGTCTCTTTGGATGTACTCGATTCCGCTGAACAAGCTCTACATCCGGATGA 300


10873R-1.IR_full       301 ACAAGGCCTTCAACGTGGACGTTCAGTTCAAGTCTAAAATGCCCATCCAACCACTTAATT 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10873R-1.in silico     301 ACAAGGCCTTCAACGTGGACGTTCAGTTCAAGTCTAAAATGCCCATCCAACCACTTAATT 360


10873R-1.IR_full       361 TGCGTGTGTTCCTTTGCTTCTCCAATGATGTGAGTGCTCCCGTGGTCCGCTGTCAAAATC 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10873R-1.in silico     361 TGCGTGTGTTCCTTTGCTTCTCCAATGATGTGAGTGCTCCCGTGGTCCGCTGTCAAAATC 420


10873R-1.IR_full       421 ACCTTAGCGTTGAGNCTTTGACGGCCAATAACGCAAAAATGCGCGAGAGCTTGCTGCGCA 480
                           |||||||||||||| |||||||||||||||||||||||||||||||||||||||||||||
10873R-1.in silico     421 ACCTTAGCGTTGAGCCTTTGACGGCCAATAACGCAAAAATGCGCGAGAGCTTGCTGCGCA 480


10873R-1.IR_full       481 GCGAGNAATCCCAACAGTGTA 501
                           ||||| |||||||||||||||
10873R-1.in silico     481 GCGAG-AATCCCAACAGTGTA 501

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_206544.1	CG33336-RB, transcript variant B (p53), mRNA
100	482	0	0	0	NM_206545.1	CG33336-RA, transcript variant A (p53), mRNA
0	0	3	5	3	NM_132641.1	CG15743-RA (CG15743), mRNA
0	0	3	5	2	NM_164387.1	CG3022-RB, transcript variant B (GABA-B-R3), mRNA
0	0	3	5	2	NM_078723.2	CG3022-RA, transcript variant A (GABA-B-R3), mRNA
0	0	3	3	6	NM_001042870.1	CG34126-RB (CG34126), mRNA
0	0	2	2	7	NM_137850.2	CG4414-RA (Ugt58Fa), mRNA
0	0	1	4	6	NM_079322.2	CG10605-RA (caup), mRNA
0	0	1	4	3	NM_135483.2	CG31712-RA (CG31712), mRNA
0	0	1	2	5	NM_140402.2	CG17689-RA, transcript variant A (CG17689), mRNA
0	0	1	2	5	NM_001038916.1	CG17689-RB, transcript variant B (CG17689), mRNA
0	0	1	2	3	NM_137802.2	CG3045-RA (CG3045), mRNA
0	0	1	2	3	NM_143297.1	CG3361-RA (mrt), mRNA
0	0	1	2	2	NM_142485.2	CG7706-RA (CG7706), mRNA
0	0	0	7	8	NM_001038853.1	CG34021-RA (CG34021), mRNA
0	0	0	6	4	NM_141435.2	CG1939-RA (CG1939), mRNA
0	0	0	5	2	NM_136024.1	CG15155-RA (CG15155), mRNA
0	0	0	4	6	NM_079198.2	CG15010-RC, transcript variant C (ago), mRNA
0	0	0	4	6	NM_168073.1	CG15010-RB, transcript variant B (ago), mRNA
0	0	0	4	6	NM_168072.1	CG15010-RA, transcript variant A (ago), mRNA
0	0	0	4	4	NM_135336.2	CG7466-RA (CG7466), mRNA
0	0	0	4	3	NM_168801.1	CG9279-RB, transcript variant B (CG9279), mRNA
0	0	0	4	3	NM_140867.1	CG9279-RA, transcript variant A (CG9279), mRNA
0	0	0	4	3	NM_142685.2	CG7034-RA (sec15), mRNA
0	0	0	4	2	NM_142577.1	CG4583-RA (ire-1), mRNA
0	0	0	4	2	NM_167309.1	CG32663-RA (CG32663), mRNA
0	0	0	3	9	NM_145194.1	CG30275-RA, transcript variant A (CG30275), mRNA
0	0	0	3	9	NM_176250.1	CG30275-RD, transcript variant D (CG30275), mRNA
0	0	0	3	9	NM_166544.1	CG30275-RC, transcript variant C (CG30275), mRNA
0	0	0	3	7	NM_132957.1	CG9059-RB, transcript variant B (CG9059), mRNA

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