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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 1082R-2 
 Symbol alpha-Est4  Full Name alpha-Esterase-4 
 CG No CG1082  Old CG No CG1082 
 Synonyms alphaE4, aE4, C, CG1082, alpha-Est4 
 Accession No (Link to NCBI) NM_079542.2 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgGAAAAGTGAGGGGAATCCTGC 
 Primer Seq. 3' 
aatctagaggtaccACCATTGAAGTTGGCCGCGTT 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 NAAAAGTGAG GGGAATNCCT GCTTAAGTCC CTATATGTCG AACAGTTTTA CGCTTTTGAT 
0061 GGCATCCCCT ATGCGGTTCC TCCGCTGGGA ACCCTGCGCT TCAAGGAGCC CCATGACCTT 
0121 AAACCATGGC ACGGGATTCG CGATTGCTCC AAGCCGCTCA GCAAGTGCCT GCAAGTGAGC 
0181 ACATTAACCA AGGAGGTGGA AGGCTCCGAG GATTGCCTTT ACCTGAACAT ATCAGTAAAA 
0241 ACTCTAAACG GTGATCCCAT GCCTGTGATG GTCTACATCC ATGGAGGCGC TTTTAAGGGC 
0301 GGAGATTCTT CGAGACGCGC CTGGGGTCCC GACTATTTTA TGAAGGAAAA CGTTGTGTAC 
0361 ATAAGCATTG GTCATCGACT GGGACCCTTA GGTTTCCTCA GTCTCAATGA TCCCGATCTT 
0421 GAAGTTCCTG GCAATGCTGG ATTGAAAGAC GTTATCTTGG CCCTcCGTTG GATCAGGGCA 
0481 AACGCGGCCA ACTTCAATGG T 
 in silico PCR Fragment 
0001 GAAAAGTGAG GGGAAT-CCT GCTTAAGTCC CTATATGACG AACAGTTTTA CGCTTTTGAT 
0061 GGCATCCCCT ATGCGGTTCC TCCGCTGGGA ACCCTGCGCT TCAAGGAGCC CCATGACCTT 
0121 AAACCATGGC ACGGGATTCG CGATTGCTCC AAGCCGCTCA GCAAGTGCCT GCAAGTGAGC 
0181 ACATTAACCA AGGAGGTGGA AGGCTCCGAG GATTGCCTTT ACCTGAACAT ATCAGTAAAA 
0241 ACTCTAAACG GTGATCCCAT GCCTGTGATG GTCTACATCC ATGGAGGCGC TTTTAAGGGC 
0301 GGAGATTCTT CGAGACGCGC CTGGGGTCCC GACTATTTTA TGAAGGAAAA CGTTGTGTAC 
0361 ATAAGCATTG GTCATCGACT GGGACCCTTA GGTTTCCTCA GTCTCAATGA TCCCGATCTT 
0421 GAAGTTCCTG GCAATGCTGG ATTGAAAGAC GTTATCTTGG CCCTCCGTTG GATCAGGGCA 
0481 AACGCGGCCA ACTTCAATGG T 
 Assemble Data 

1082R-2.IR_full        1   AAAAGTGAGGGGAATNCCTGCTTAAGTCCCTATATGTCGAACAGTTTTACGCTTTTGAT 59
                           ||||||||||||||| |||||||||||||||||||| ||||||||||||||||||||||
1082R-2.in silico      1   AAAAGTGAGGGGAAT-CCTGCTTAAGTCCCTATATGACGAACAGTTTTACGCTTTTGAT 59


1082R-2.IR_full       61  GGCATCCCCTATGCGGTTCCTCCGCTGGGAACCCTGCGCTTCAAGGAGCCCCATGACCTT 119
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1082R-2.in silico     61  GGCATCCCCTATGCGGTTCCTCCGCTGGGAACCCTGCGCTTCAAGGAGCCCCATGACCTT 119


1082R-2.IR_full       121 AAACCATGGCACGGGATTCGCGATTGCTCCAAGCCGCTCAGCAAGTGCCTGCAAGTGAGC 179
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1082R-2.in silico     121 AAACCATGGCACGGGATTCGCGATTGCTCCAAGCCGCTCAGCAAGTGCCTGCAAGTGAGC 179


1082R-2.IR_full       181 ACATTAACCAAGGAGGTGGAAGGCTCCGAGGATTGCCTTTACCTGAACATATCAGTAAAA 239
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1082R-2.in silico     181 ACATTAACCAAGGAGGTGGAAGGCTCCGAGGATTGCCTTTACCTGAACATATCAGTAAAA 239


1082R-2.IR_full       241 ACTCTAAACGGTGATCCCATGCCTGTGATGGTCTACATCCATGGAGGCGCTTTTAAGGGC 299
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1082R-2.in silico     241 ACTCTAAACGGTGATCCCATGCCTGTGATGGTCTACATCCATGGAGGCGCTTTTAAGGGC 299


1082R-2.IR_full       301 GGAGATTCTTCGAGACGCGCCTGGGGTCCCGACTATTTTATGAAGGAAAACGTTGTGTAC 359
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1082R-2.in silico     301 GGAGATTCTTCGAGACGCGCCTGGGGTCCCGACTATTTTATGAAGGAAAACGTTGTGTAC 359


1082R-2.IR_full       361 ATAAGCATTGGTCATCGACTGGGACCCTTAGGTTTCCTCAGTCTCAATGATCCCGATCTT 419
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1082R-2.in silico     361 ATAAGCATTGGTCATCGACTGGGACCCTTAGGTTTCCTCAGTCTCAATGATCCCGATCTT 419


1082R-2.IR_full       421 GAAGTTCCTGGCAATGCTGGATTGAAAGACGTTATCTTGGCCCTCCGTTGGATCAGGGCA 479
                          ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1082R-2.in silico     421 GAAGTTCCTGGCAATGCTGGATTGAAAGACGTTATCTTGGCCCTCCGTTGGATCAGGGCA 479


1082R-2.IR_full       481 AACGCGGCCAACTTCAATGGT 500
                          |||||||||||||||||||||
1082R-2.in silico     481 AACGCGGCCAACTTCAATGGT 500



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_079542.2  CG1082-RA (alpha-Est4), mRNA 
0   0  6  23  64  NR_001368.2  CG1082-RA (alpha-Est4), mRNA, mRNA 
0   0  4  8  13  NM_079545.2  CG1031-RA (alpha-Est1), mRNA 
0   0  4  8  8  NM_137834.1  CG6018-RA (CG6018), mRNA 
0   0  2  9  13  NM_135401.1  CG9289-RA (CG9289), mRNA 
0   0  2  6  8  NM_057949.3  CG10270-RA (D19B), mRNA 
0   0  2  2  6  NM_141786.2  CG4757-RA (CG4757), mRNA 
0   0  1  8  12  NM_080523.2  CG9858-RA (clt), mRNA 
0   0  1  3  4  NM_139965.2  CG7127-RA (exo70), mRNA 
0   0  1  3  2  NM_136557.2  CG8635-RA (CG8635), mRNA 
0   0  1  3  1  NM_164908.2  CG5640-RC, transcript variant C (CG5640), mRNA 
0   0  1  3  1  NM_164907.2  CG5640-RB, transcript variant B (CG5640), mRNA 
0   0  1  3  1  NM_135524.3  CG5640-RA, transcript variant A (CG5640), mRNA 
0   0  1  2  2  NM_143562.1  CG12069-RA (CG12069), mRNA 
0   0  0  11  8  NM_079034.2  CG8425-RA (Jhe), mRNA 
0   0  0  10  10  NM_165129.1  CG3903-RE, transcript variant E (Gli), mRNA 
0   0  0  10  10  NM_165127.1  CG3903-RC, transcript variant C (Gli), mRNA 
0   0  0  10  10  NM_165128.1  CG3903-RD, transcript variant D (Gli), mRNA 
0   0  0  10  10  NM_057254.3  CG3903-RA, transcript variant A (Gli), mRNA 
0   0  0  10  10  NM_165130.1  CG3903-RB, transcript variant B (Gli), mRNA 
0   0  0  8  14  NM_079534.1  CG1128-RB, transcript variant B (alpha-Est9), mRNA 
0   0  0  8  14  NM_169187.1  CG1128-RA, transcript variant A (alpha-Est9), mRNA 
0   0  0  7  11  NM_130733.1  CG6414-RA (CG6414), mRNA 
0   0  0  7  8  NM_079544.3  CG2505-RA (alpha-Est2), mRNA 
0   0  0  7  3  NM_137125.2  CG12869-RA (CG12869), mRNA 
0   0  0  5  11  NM_079537.2  CG1112-RA, transcript variant A (alpha-Est7), mRNA 
0   0  0  5  9  NM_169188.1  CG1112-RB, transcript variant B (alpha-Est7), mRNA 
0   0  0  4  5  NM_143558.1  CG2267-RA (CG2267), mRNA 
0   0  0  4  2  NM_079579.2  CG6342-RA (Irp-1B), mRNA 
0   0  0  3  14  NM_137241.1  CG8424-RA (Jhedup), mRNA 

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