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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
10800R-1 |
| Symbol |
Rca1 |
Full Name |
Regulator of cyclin A1 |
| CG No |
CG10800 |
Old CG No |
CG10800 |
| Synonyms |
152549_at, rca1, CG10800, P3300, S(rux)2A, l(2)03300, Rca1 |
| Accession No (Link to NCBI) |
NM_057866.2 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Chen ZS, Wong AKY, Cheng TC, Koon AC, Chan HYE.
FipoQ/FBXO33, a Cullin-1-based ubiquitin ligase complex component modulates ubiquitination and solubility of polyglutamine disease protein.
J Neurochem (2019) 149(6) 781-798
[
PubMed ID = 30685895
]
[
RRC reference
]
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Umetsu D, Dunst S, Dahmann C.
An RNA interference screen for genes required to shape the anteroposterior compartment boundary in Drosophila identifies the Eph receptor.
PLoS One (2014) 9(12) e114340
[
PubMed ID = 25473846
]
[
RRC reference
]
Zielke N, Querings S, Rottig C, Lehner C, Sprenger F.
The anaphase-promoting complex/cyclosome (APC/C) is required for rereplication control in endoreplication cycles.
Genes Dev (2008) 22(12) 1690-703
[
PubMed ID = 18559483
]
[
RRC reference
]
Rogulja D, Young MW.
Control of sleep by cyclin A and its regulator.
Science (2012) 335(6076) 1617-21
[
PubMed ID = 22461610
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgCCGAGGGTCATCCTTCGAGTT |
| Primer Seq. 3' |
aatctagaggtaccTCATCACGTCCAGCGTGTGGG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 CCGAGGGTCA TCCTTCGAGT TGGAGATGAA CGAGTCTGGC TACACATCCT TCCTGGCGCT
0061 GCACAATTCC ACCGCGGAGA CGCCATTTTT ATTGGAGGAC GCTGAGGGCG AAAACTGTCG
0121 CAATGCATCG AATACCACAA CATTCTTTCG GGGGCTGAAC ACGCCCAGTG GCCACCAGGA
0181 GCAGGACCTT TACTGGGGCA AGCCCNTATC CCAGAACACA GCCCCAAAAG AAATTTTCCG
0241 CGGAGGAGGA GCCTTTCTCT ATGACTCCGC GTCTGCAGGA TGAGCATAGT CTGCCCAAGC
0301 GACGCAAGAA ACACTTTCAA TCGCCACACA GTAGCCCCAA GAAGTCCAAA AAGCTGCTCT
0361 TTCCCCACAT AGAAGAACCG CCCAAGAATC GCTTCTACGG CGGTGTCGAA AAGCTGGACA
0421 TCGTGGCCAA GCTGGCGCAA TGGCAACCGG CACTGCAGTg CATACTGCGT CATGTGGGCG
0481 CCCACACGCT GGACGTGATG A |
| in silico PCR Fragment |
0001 CCGAGGGTCA TCCTTCGAGT TGGAGATGAA CGAGTCTGGC TACACATCCT TCCTGGCGCT
0061 GCACAATTCC ACCGCGGAGA CGCCATTTTT ATTGGAGGAC GCTGAGGGCG AAAACTGTCG
0121 CAATGCATCG AATACCACAA CATTCTTTCG GGGGCTGAAC ACGCCCAGTG GCCACCAGGA
0181 GCAGGACCTT TACTGGGGCA AGCCC-TATC CCAGAACACA GCCCCAAAAG AAATTTTCCG
0241 CGGAGGAGGA GCCTTTCTCT ATGACTCCGC GTCTGCAGGA TGAGCATAGT CTGCCCAAGC
0301 GACGCAAGAA ACACTTTCAA TCGCCACACA GTAGCCCCAA GAAGTCCAAA AAGCTGCTCT
0361 TTCCCCACAT AGAAGAACCG CCCAAGAATC GCTTCTACGG CGGTGTCGAA AAGCTGGATA
0421 TCGTGGCCAA GCTGGCGCAA TGGCAACCGG CACTGCAGTG CATACTGCGT CATGTGGGCG
0481 CCCACACGCT GGACGTGATG A |
| Assemble Data |
10800R-1.IR_full 1 CCGAGGGTCATCCTTCGAGTTGGAGATGAACGAGTCTGGCTACACATCCTTCCTGGCGCT 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10800R-1.in silico 1 CCGAGGGTCATCCTTCGAGTTGGAGATGAACGAGTCTGGCTACACATCCTTCCTGGCGCT 60
10800R-1.IR_full 61 GCACAATTCCACCGCGGAGACGCCATTTTTATTGGAGGACGCTGAGGGCGAAAACTGTCG 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10800R-1.in silico 61 GCACAATTCCACCGCGGAGACGCCATTTTTATTGGAGGACGCTGAGGGCGAAAACTGTCG 120
10800R-1.IR_full 121 CAATGCATCGAATACCACAACATTCTTTCGGGGGCTGAACACGCCCAGTGGCCACCAGGA 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10800R-1.in silico 121 CAATGCATCGAATACCACAACATTCTTTCGGGGGCTGAACACGCCCAGTGGCCACCAGGA 180
10800R-1.IR_full 181 GCAGGACCTTTACTGGGGCAAGCCCNTATCCCAGAACACAGCCCCAAAAGAAATTTTCCG 240
||||||||||||||||||||||||| ||||||||||||||||||||||||||||||||||
10800R-1.in silico 181 GCAGGACCTTTACTGGGGCAAGCCC-TATCCCAGAACACAGCCCCAAAAGAAATTTTCCG 240
10800R-1.IR_full 241 CGGAGGAGGAGCCTTTCTCTATGACTCCGCGTCTGCAGGATGAGCATAGTCTGCCCAAGC 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10800R-1.in silico 241 CGGAGGAGGAGCCTTTCTCTATGACTCCGCGTCTGCAGGATGAGCATAGTCTGCCCAAGC 300
10800R-1.IR_full 301 GACGCAAGAAACACTTTCAATCGCCACACAGTAGCCCCAAGAAGTCCAAAAAGCTGCTCT 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10800R-1.in silico 301 GACGCAAGAAACACTTTCAATCGCCACACAGTAGCCCCAAGAAGTCCAAAAAGCTGCTCT 360
10800R-1.IR_full 361 TTCCCCACATAGAAGAACCGCCCAAGAATCGCTTCTACGGCGGTGTCGAAAAGCTGGACA 420
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||| |
10800R-1.in silico 361 TTCCCCACATAGAAGAACCGCCCAAGAATCGCTTCTACGGCGGTGTCGAAAAGCTGGATA 420
10800R-1.IR_full 421 TCGTGGCCAAGCTGGCGCAATGGCAACCGGCACTGCAGTGCATACTGCGTCATGTGGGCG 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10800R-1.in silico 421 TCGTGGCCAAGCTGGCGCAATGGCAACCGGCACTGCAGTGCATACTGCGTCATGTGGGCG 480
10800R-1.IR_full 481 CCCACACGCTGGACGTGATGA 501
|||||||||||||||||||||
10800R-1.in silico 481 CCCACACGCTGGACGTGATGA 501
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
4 |
NM_057866.2 |
CG10800-RA (Rca1), mRNA |
| 0 |
0 |
3 |
2 |
4 |
NM_136553.2 |
CG8639-RA (Cirl), mRNA |
| 0 |
0 |
2 |
4 |
3 |
NM_079357.2 |
CG7250-RA (Toll-6), mRNA |
| 0 |
0 |
2 |
4 |
3 |
NM_136043.2 |
CG10413-RA (CG10413), mRNA |
| 0 |
0 |
2 |
3 |
13 |
NM_132539.2 |
CG1559-RA (Upf1), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_057740.3 |
CG1912-RA (Gycalpha99B), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_140897.2 |
CG8103-RA, transcript variant A (Mi-2), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_001014591.1 |
CG8103-RB, transcript variant B (Mi-2), mRNA |
| 0 |
0 |
2 |
2 |
5 |
NM_132633.2 |
CG4332-RA (CG4332), mRNA |
| 0 |
0 |
1 |
5 |
6 |
NM_130725.2 |
CG15239-RA (CG15239), mRNA |
| 0 |
0 |
1 |
5 |
3 |
NM_132903.1 |
CG9906-RA (CG9906), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_176234.1 |
CG33041-RA (CG33041), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_142103.1 |
CG14846-RA (CG14846), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_137992.1 |
CG5549-RA (CG5549), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_132012.1 |
CG15776-RA (CG15776), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NR_002088.1 |
CG15776-RA (CG15776), mRNA, mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_132850.2 |
CG8995-RA (PGRP-LE), mRNA |
| 0 |
0 |
0 |
5 |
5 |
NM_142043.1 |
CG9637-RA (Task6), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_176247.1 |
CG33143-RB, transcript variant B (CG33143), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_176246.1 |
CG33143-RC, transcript variant C (CG33143), mRNA |
| 0 |
0 |
0 |
4 |
6 |
NM_132040.1 |
CG15766-RA (CG15766), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_165836.1 |
CG7776-RB, transcript variant B (E(Pc)), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_078974.2 |
CG7776-RA, transcript variant A (E(Pc)), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_132692.2 |
CG10992-RA (CG10992), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_137339.2 |
CG9646-RA (CG9646), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_141108.1 |
CG7414-RA (CG7414), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_143480.2 |
CG18041-RA (CG18041), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_141378.2 |
CG1155-RA (Osi14), mRNA |
| 0 |
0 |
0 |
3 |
8 |
NM_139436.1 |
CG15822-RC (CG15822), mRNA |
| 0 |
0 |
0 |
3 |
6 |
NM_078729.2 |
CG12193-RA (Or22a), mRNA |
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