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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
10776R-2 |
| Symbol |
wit |
Full Name |
wishful thinking |
| CG No |
CG10776 |
Old CG No |
CG10776 |
| Synonyms |
CG10776, STK-D, ALK3/BMPRII, l(3)64Aa, 1262/15, SE20, l(3)SH12, l(3)S126215, Stk-D, wit, Wit |
| Accession No (Link to NCBI) |
NM_079953.3 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
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Reference
[Please submit your publication]
|
Swarup S, Pradhan-Sundd T, Verheyen EM.
Genome-wide identification of phospho-regulators of Wnt signaling in Drosophila.
Development (2015) 142(8) 1502-15
[
PubMed ID = 25852200
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
Mummery-Widmer JL, Yamazaki M, Stoeger T, Novatchkova M, Bhalerao S, Chen D, Dietzl G, Dickson BJ, Knoblich JA.
Genome-wide analysis of Notch signalling in Drosophila by transgenic RNAi.
Nature (2009) 458(7241) 987-92
[
PubMed ID = 19363474
]
[
RRC reference
]
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| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgCCTGTTCCCAATCGCCAGTAT |
| Primer Seq. 3' |
aatctagaggtaccCCGATTGTGAGGGCTGTGAGT |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 CCTGtTCCCA ATCGCCAGTA TAGCTGTATG AGCTACCAGG AGGACGACAA CTCATTTCAC
0061 GATGATGATG GCGATCAGGA CTCTAGTGGC GAGCTGCAGG AGCNAGCAGG TGGAGTCCAC
0121 TCCGATTCCG AGCGAGCCAC ATAGACGCAC CTGCCCAGAT GGCNTACACN TTTCTGTTTC
0181 ACCATCTGGA ACCAGACGGC CAATGGAGCG CGGGTTGTTA AACAGGGTTG CTGGAAGGAC
0241 AACACGGATC GCACTTCCAT TTGCAGCCAG TCGGAATGTA CCAGCTCGGC TCCCACCTCG
0301 AAGACCAGTT CTTTGTACTA CTGTTGCTGT TCGGGAGGAG TGTNCAATGC CCAATATTCC
0361 GTGGTCGAAC CGGCACCTCT GGAACTGGGC TCCAATGAGG GACGGACTTC GATCACAAAT
0421 CGGGCGACAG AAAAACAGCA CCAGTCCTTT TTGGCCAGTA CAATGCTGGG NCTTGCCGGT
0481 GGACTCACAG CCCTCACAAT CGG |
| in silico PCR Fragment |
0001 CCTGTTCCCA ATCGCCAGTA TAGCTGTATG AGCTACCAGG AGGACGACAA CTCATTTCAC
0061 GATGATGATG GCGATCAGGA CTCTAGTGGC GAGCTGCAGG AGC-AGCAGG TGGAGTCCAC
0121 TCCGATTCCG AGCGAGCCAC ATAGACGCAC CTGCCCAGAT GGC-TACAC- TTTCTGTTTC
0181 ACCATCTGGA ACCAGACGGC CAATGGAGCG CGGGTTGTTA AACAGGGTTG CTGGAAGGAC
0241 AACACGGATC GCACTTCCAT TTGCAGCCAG TCGGAATGTA CCAGCTCGGC TCCCACCTCG
0301 AAGACCAGTT CTTTGTACTA CTGTTGCTGT TCGGGAGGAG TGTGCAATGC CCAATATTCC
0361 GTGGTCGAAC CGGCACCTCT GGAACTGGGC TCCAATGAGG GACGGACTTC GATCACAAAT
0421 CGGGCGACAG AAAAACAGCA CCAGTCCTTT TTGGCCAGTA CAATGCTGGG CCTTGCCGGT
0481 GGACTCACAG CCCTCACAAT CGG |
| Assemble Data |
10776R-2.IR_full 1 CCTGTTCCCAATCGCCAGTATAGCTGTATGAGCTACCAGGAGGACGACAACTCATTTCAC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10776R-2.in silico 1 CCTGTTCCCAATCGCCAGTATAGCTGTATGAGCTACCAGGAGGACGACAACTCATTTCAC 60
10776R-2.IR_full 61 GATGATGATGGCGATCAGGACTCTAGTGGCGAGCTGCAGGAGCNAGCAGGTGGAGTCCAC 120
||||||||||||||||||||||||||||||||||||||||||| ||||||||||||||||
10776R-2.in silico 61 GATGATGATGGCGATCAGGACTCTAGTGGCGAGCTGCAGGAGC-AGCAGGTGGAGTCCAC 120
10776R-2.IR_full 121 TCCGATTCCGAGCGAGCCACATAGACGCACCTGCCCAGATGGCNTACACNTTTCTGTTTC 180
||||||||||||||||||||||||||||||||||||||||||| ||||| ||||||||||
10776R-2.in silico 121 TCCGATTCCGAGCGAGCCACATAGACGCACCTGCCCAGATGGC-TACAC-TTTCTGTTTC 180
10776R-2.IR_full 181 ACCATCTGGAACCAGACGGCCAATGGAGCGCGGGTTGTTAAACAGGGTTGCTGGAAGGAC 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10776R-2.in silico 181 ACCATCTGGAACCAGACGGCCAATGGAGCGCGGGTTGTTAAACAGGGTTGCTGGAAGGAC 240
10776R-2.IR_full 241 AACACGGATCGCACTTCCATTTGCAGCCAGTCGGAATGTACCAGCTCGGCTCCCACCTCG 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10776R-2.in silico 241 AACACGGATCGCACTTCCATTTGCAGCCAGTCGGAATGTACCAGCTCGGCTCCCACCTCG 300
10776R-2.IR_full 301 AAGACCAGTTCTTTGTACTACTGTTGCTGTTCGGGAGGAGTGTNCAATGCCCAATATTCC 360
||||||||||||||||||||||||||||||||||||||||||| ||||||||||||||||
10776R-2.in silico 301 AAGACCAGTTCTTTGTACTACTGTTGCTGTTCGGGAGGAGTGTGCAATGCCCAATATTCC 360
10776R-2.IR_full 361 GTGGTCGAACCGGCACCTCTGGAACTGGGCTCCAATGAGGGACGGACTTCGATCACAAAT 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10776R-2.in silico 361 GTGGTCGAACCGGCACCTCTGGAACTGGGCTCCAATGAGGGACGGACTTCGATCACAAAT 420
10776R-2.IR_full 421 CGGGCGACAGAAAAACAGCACCAGTCCTTTTTGGCCAGTACAATGCTGGGNCTTGCCGGT 480
|||||||||||||||||||||||||||||||||||||||||||||||||| |||||||||
10776R-2.in silico 421 CGGGCGACAGAAAAACAGCACCAGTCCTTTTTGGCCAGTACAATGCTGGGCCTTGCCGGT 480
10776R-2.IR_full 481 GGACTCACAGCCCTCACAATCGG 503
|||||||||||||||||||||||
10776R-2.in silico 481 GGACTCACAGCCCTCACAATCGG 503
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_079953.3 |
CG10776-RA (wit), mRNA |
| 0 |
0 |
3 |
4 |
2 |
NM_078945.2 |
CG2346-RA (Fmrf), mRNA |
| 0 |
0 |
3 |
3 |
20 |
NM_164401.3 |
CG31795-RB, transcript variant B (ia2), mRNA |
| 0 |
0 |
3 |
3 |
19 |
NM_134718.4 |
CG31795-RA, transcript variant A (ia2), mRNA |
| 0 |
0 |
3 |
3 |
4 |
NM_167302.1 |
CG1841-RB, transcript variant B (Tango10), mRNA |
| 0 |
0 |
3 |
3 |
4 |
NM_132515.2 |
CG1841-RA, transcript variant A (Tango10), mRNA |
| 0 |
0 |
3 |
3 |
3 |
NM_078605.1 |
CG6211-RA (gce), mRNA |
| 0 |
0 |
3 |
2 |
8 |
NM_169533.1 |
CG9412-RA, transcript variant A (rin), mRNA |
| 0 |
0 |
3 |
2 |
8 |
NM_080168.2 |
CG9412-RB, transcript variant B (rin), mRNA |
| 0 |
0 |
3 |
2 |
8 |
NM_169531.1 |
CG9412-RD, transcript variant D (rin), mRNA |
| 0 |
0 |
3 |
2 |
8 |
NM_169532.1 |
CG9412-RE, transcript variant E (rin), mRNA |
| 0 |
0 |
3 |
2 |
8 |
NM_169530.1 |
CG9412-RC, transcript variant C (rin), mRNA |
| 0 |
0 |
3 |
2 |
2 |
NM_140984.1 |
CG5104-RB (CG5104), mRNA |
| 0 |
0 |
2 |
8 |
12 |
NM_165086.1 |
CG15288-RB, transcript variant B (wb), mRNA |
| 0 |
0 |
2 |
8 |
12 |
NM_057442.3 |
CG15288-RA, transcript variant A (wb), mRNA |
| 0 |
0 |
2 |
4 |
3 |
NM_133133.1 |
CG7990-RA, transcript variant A (CG7990), mRNA |
| 0 |
0 |
2 |
4 |
3 |
NM_167640.1 |
CG7990-RB, transcript variant B (CG7990), mRNA |
| 0 |
0 |
2 |
4 |
2 |
NM_136794.1 |
CG13231-RA (CG13231), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_130497.2 |
CG13363-RA (Suv4-20), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_079756.2 |
CG6383-RA, transcript variant A (crb), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_001043286.1 |
CG6383-RB, transcript variant B (crb), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_140050.2 |
CG4022-RA (CG4022), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_167480.1 |
CG9209-RC, transcript variant C (vap), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_206759.1 |
CG9209-RD, transcript variant D (vap), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_167481.2 |
CG9209-RA, transcript variant A (vap), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_078637.2 |
CG9209-RB, transcript variant B (vap), mRNA |
| 0 |
0 |
1 |
6 |
15 |
NM_080110.2 |
CG32356-RA, transcript variant A (ImpE1), mRNA |
| 0 |
0 |
1 |
6 |
5 |
NM_132667.1 |
CG15756-RA (CG15756), mRNA |
| 0 |
0 |
1 |
5 |
10 |
NM_132131.2 |
CG14435-RA (CG14435), mRNA |
| 0 |
0 |
1 |
4 |
5 |
NM_166516.1 |
CG11170-RB (CG11170), mRNA |
|
| Wing Disc |
GFP
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Puc
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Caspase
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DAPI
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Merge
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Figure
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JNK signal
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Caspase-3 signal
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