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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 10725R-1 
 Symbol CG10725  Full Name CG10725 
 CG No CG10725  Old CG No CG10725 
 Synonyms CG10725 
 Accession No (Link to NCBI) NM_140390.2 
 Inserted Chr. ll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgTGATAAGTCGCCTGACCCTTT 
 Primer Seq. 3' 
aatctagaggtaccAGTACTTGTCACAGCGAGCCT 
 Predicted Fragment Size 
480 
 IR fragment full Seq 
0001 TGATAAGTCG CCTGACCCTT TTGGCACTGG TTGCCCTGCT TGGAAGCTGT AGCGCNTGCT 
0061 GATGGCGATG TCAATGTCTG CTCCAATGTG GTCAACAATC TCTTTGTTCC CCAGGTGGGC 
0121 AACTGTAGCA AGTACTTCCT GTGCATGAAT GAGATAGCTG TACCCCGTGA ATGCCCCACG 
0181 GACTACTACT TCGATGCGAG GGATCAGGAG TGTGTTCCCC TGATGGAGGT CGAGTGTATT 
0241 GGATCCTGTA AGAACCGTGG TTTGTCATCG TTCTGTTACG ATCGCACGTG CACCAAGTAC 
0301 GTTCTCTGCT TCGACGGCAC TCCGGTGATC CGTCAGTGCT CCGATGGACT GCAGTACAAC 
0361 GCCCTGACCG ATCGCTGTGA CTATCCGCAG TATGTCGGAC TGCGTGGACA ATCTGTGCAG 
0421 CCGGAATAAT AATCCGGATG ACATCGTTTT CATACCCAGC AAGGCTCGCT GTGACAAGTA 
0481 CT 
 in silico PCR Fragment 
0001 TGATAAGTCG CCTGACCCTT TTGGCACTGG TTGCCCTGCT TGGAAGCTGT AGCGC-TGCT 
0061 GATGGCGATG TCAATGTCTG CTCCAATGTG GTCAACAATC TCTTTGTTCC CCAGGTGGGC 
0121 AACTGTAGCA AGTACTTCCT GTGCATGAAT GAGATAGCTG TACCCCGTGA ATGCCCCACG 
0181 GACTACTACT TCGATGCGAG GGATCAGGAG TGTGTTCCCC TGATGGAGGT CGAGTGTATT 
0241 GGATCCTGTA AGAACCGTGG TTTGTCATCG TTCTGTTACG ATCGCACGTG CACCAAGTAC 
0301 GTTCTCTGCT TCGACGGCAC TCCGGTGATC CGTCAGTGCT CCGATGGACT GCAGTACAAC 
0361 GCCCTGACCG ATCGCTGTGA CTATCCGCAG TATGTC-GAC TGCGTGGACA ATCTGTGCAG 
0421 CCGGAATAAT AATCCGGATG ACATCGTTTT CATACCCAGC AAGGCTCGCT GTGACAAGTA 
0481 CT 
 Assemble Data 

10725R-1.IR_full       1   TGATAAGTCGCCTGACCCTTTTGGCACTGGTTGCCCTGCTTGGAAGCTGTAGCGCNTGCT 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||| ||||
10725R-1.in silico     1   TGATAAGTCGCCTGACCCTTTTGGCACTGGTTGCCCTGCTTGGAAGCTGTAGCGC-TGCT 60


10725R-1.IR_full       61  GATGGCGATGTCAATGTCTGCTCCAATGTGGTCAACAATCTCTTTGTTCCCCAGGTGGGC 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10725R-1.in silico     61  GATGGCGATGTCAATGTCTGCTCCAATGTGGTCAACAATCTCTTTGTTCCCCAGGTGGGC 120


10725R-1.IR_full       121 AACTGTAGCAAGTACTTCCTGTGCATGAATGAGATAGCTGTACCCCGTGAATGCCCCACG 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10725R-1.in silico     121 AACTGTAGCAAGTACTTCCTGTGCATGAATGAGATAGCTGTACCCCGTGAATGCCCCACG 180


10725R-1.IR_full       181 GACTACTACTTCGATGCGAGGGATCAGGAGTGTGTTCCCCTGATGGAGGTCGAGTGTATT 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10725R-1.in silico     181 GACTACTACTTCGATGCGAGGGATCAGGAGTGTGTTCCCCTGATGGAGGTCGAGTGTATT 240


10725R-1.IR_full       241 GGATCCTGTAAGAACCGTGGTTTGTCATCGTTCTGTTACGATCGCACGTGCACCAAGTAC 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10725R-1.in silico     241 GGATCCTGTAAGAACCGTGGTTTGTCATCGTTCTGTTACGATCGCACGTGCACCAAGTAC 300


10725R-1.IR_full       301 GTTCTCTGCTTCGACGGCACTCCGGTGATCCGTCAGTGCTCCGATGGACTGCAGTACAAC 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10725R-1.in silico     301 GTTCTCTGCTTCGACGGCACTCCGGTGATCCGTCAGTGCTCCGATGGACTGCAGTACAAC 360


10725R-1.IR_full       361 GCCCTGACCGATCGCTGTGACTATCCGCAGTATGTCGGACTGCGTGGACAATCTGTGCAG 420
                           |||||||||||||||||||||||||||||||||||| |||||||||||||||||||||||
10725R-1.in silico     361 GCCCTGACCGATCGCTGTGACTATCCGCAGTATGTC-GACTGCGTGGACAATCTGTGCAG 420


10725R-1.IR_full       421 CCGGAATAATAATCCGGATGACATCGTTTTCATACCCAGCAAGGCTCGCTGTGACAAGTA 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10725R-1.in silico     421 CCGGAATAATAATCCGGATGACATCGTTTTCATACCCAGCAAGGCTCGCTGTGACAAGTA 480


10725R-1.IR_full       481 CT 482
                           ||
10725R-1.in silico     481 CT 482



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   462  0  0  1  NM_140390.2  CG10725-RB (CG10725), mRNA 
0   0  2  3  6  NM_138200.2  CG12030-RA (CG12030), mRNA 
0   0  1  5  3  NM_135696.2  CG6792-RA (CG6792), mRNA 
0   0  1  5  2  NM_133113.1  CG7378-RA (CG7378), mRNA 
0   0  1  4  25  NM_140389.1  CG10154-RA (CG10154), mRNA 
0   0  1  4  5  NM_134539.1  CG1631-RA (CG1631), mRNA 
0   0  1  2  7  NM_143496.3  CG15523-RA (CG15523), mRNA 
0   0  1  2  4  NM_079015.2  CG8404-RA (Sox15), mRNA 
0   0  1  2  4  NM_134574.1  CG1304-RA (CG1304), mRNA 
0   0  1  2  3  NM_165657.1  CG8046-RA, transcript variant A (CG8046), mRNA 
0   0  1  2  3  NM_136621.2  CG8046-RB, transcript variant B (CG8046), mRNA 
0   0  1  2  3  NM_140259.1  CG5917-RA (CG5917), mRNA 
0   0  1  2  3  NM_168544.1  CG32121-RA (CG32121), mRNA 
0   0  1  2  2  NM_165511.2  CG30385-RA (CG30385), mRNA 
0   0  0  6  6  NM_139680.1  CG17150-RA, transcript variant A (CG17150), mRNA 
0   0  0  6  5  NM_140933.1  CG7017-RA (CG7017), mRNA 
0   0  0  4  116  NM_175960.3  CG33196-RB (dp), mRNA 
0   0  0  4  19  NM_140276.2  CG6947-RA (CG6947), mRNA 
0   0  0  4  3  NM_140852.1  CG14087-RA (CG14087), mRNA 
0   0  0  4  2  NM_079805.2  CG5954-RA, transcript variant A (l(3)mbt), mRNA 
0   0  0  4  2  NM_170330.1  CG5954-RB, transcript variant B (l(3)mbt), mRNA 
0   0  0  3  11  NM_001032051.1  CG33715-RD, transcript variant D (Msp-300), mRNA 
0   0  0  3  7  NM_165275.1  CG10702-RB, transcript variant B (CG10702), mRNA 
0   0  0  3  7  NM_165274.1  CG10702-RA, transcript variant A (CG10702), mRNA 
0   0  0  3  7  NM_165276.1  CG10702-RC, transcript variant C (CG10702), mRNA 
0   0  0  3  6  NM_140931.1  CG7290-RA (CG7290), mRNA 
0   0  0  3  4  NM_001042862.1  CG7337-RC, transcript variant C (CG7337), mRNA 
0   0  0  3  4  NM_079192.2  CG15811-RA (Rop), mRNA 
0   0  0  3  4  NM_141417.2  CG1315-RA (CG1315), mRNA 
0   0  0  3  4  NM_140388.1  CG10713-RA (CG10713), mRNA 
 Wing Disc
GFP
 Puc
 Caspase
DAPI
 Merge
 Figure
JNK signal
N
 
DB
 
DC
+- 
DM
+ 
VM
+- 
VB
 
Caspase-3 signal
N
+ 
DB
+ 
DC
+ 
DM
 
VM
 
VB
 
Dcompartment   Penetrance
     

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