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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
10697R-2 |
| Symbol |
Ddc |
Full Name |
Dopa decarboxylase |
| CG No |
CG10697 |
Old CG No |
CG10697 |
| Synonyms |
DDC, CG10697, unnamed, ddc, AADC, 5-HT, fDDC, DdcDm, l(2)k02104, l(2)37Ch, l(2)37Bl, Ddc |
| Accession No (Link to NCBI) |
NM_078876.4 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
pupal lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Cho Y, Lai CM, Lin KY, Hsu HJ.
A Targeted RNAi Screen Reveals Drosophila Female-Sterile Genes That Control the Size of Germline Stem Cell Niche During Development.
G3 (Bethesda) (2018) 8(7) 2345-2354
[
PubMed ID = 29764959
]
[
RRC reference
]
Minami R, Sato C, Yamahama Y, Kubo H, Hariyama T, Kimura KI.
An RNAi Screen for Genes Involved in Nanoscale Protrusion Formation on Corneal Lens in Drosophila melanogaster.
Zoolog Sci (2016) 33(6) 583-591
[
PubMed ID = 27927092
]
[
RRC reference
]
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGCGCCGGAGTTCAAGGATTTT |
| Primer Seq. 3' |
aatctagaggtaccTCCTTCACCTCCTTCAACTTC |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 GCGCCGGAGT TCAAGGATTT TGCCAAGACA ATGGTCGACT TTATAGCCGA ATATCTGGAG
0061 AATATACGCG AAAGGCGTGT TCTGCCGGAA GTGAAGCCTG GCTACCTGAA GCCACTGATT
0121 CCGGATGCTG CGCCCGAGAA GCCGGAGAAG TGGCAGGATG TGATGCAGGA CATCGAGCGA
0181 GTCATCATGC CGGGCGTGAC ACACTGGCAC AGTCCCAAGT TTCATGCCTA CTTCCCCACG
0241 GCCAACTCGT ATCCAGCGAT CGTTGCGGAC ATGCTGAGTG GAGCGATTGC CTGCATCGGA
0301 TTCACGTGGA TCGCCAGTCC CGCGTGCACG GAACTCGAGG TGGTCATGAT GGATTGGCTG
0361 GGCAAGATGC TGGAGCTGCC GGCAGAGTTC CTGGCCTGTT CGGGCGGCAA GGGTGGCGGT
0421 GTCATCCAGG GCACGGCCAG TGAGTCCACA CTGGTGGCTC TGCTGGGAGC CAAGGCCAAG
0481 AAGTTGAAGG AGGTGAAGGA |
| in silico PCR Fragment |
0001 GCGCCGGAGT TCAAGGATTT TGCCAAGACA ATGGTCGACT TTATAGCCGA ATATCTGGAG
0061 AATATACGCG AAAGGCGCGT TCTGCCGGAA GTGAAGCCTG GCTACCTGAA GCCATTGATT
0121 CCGGATGCTG CGCCCGAGAA GCCGGAGAAG TGGCAGGATG TGATGCAGGA CATCGAGCGA
0181 GTCATCATGC CGGGCGTGAC ACACTGGCAC AGTCCCAAGT TTCATGCCTA CTTCCCCACG
0241 GCCAACTCGT ATCCAGCGAT CGTTGCGGAC ATGCTGAGTG GAGCGATTGC CTGCATCGGA
0301 TTCACGTGGA TCGCCAGTCC CGCGTGCACG GAACTCGAGG TGGTCATGAT GGATTGGCTG
0361 GGCAAGATGC TGGAGCTGCC GGCAGAGTTC CTGGCCTGTT CGGGCGGCAA GGGTGGCGGT
0421 GTCATCCAGG GCACGGCCAG TGAGTCCACA CTGGTGGCTC TGCTGGGAGC CAAGGCCAAG
0481 AAGTTGAAGG AGGTGAAGGA |
| Assemble Data |
10697R-2.IR_full 1 GCGCCGGAGTTCAAGGATTTTGCCAAGACAATGGTCGACTTTATAGCCGAATATCTGGAG 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10697R-2.in silico 1 GCGCCGGAGTTCAAGGATTTTGCCAAGACAATGGTCGACTTTATAGCCGAATATCTGGAG 60
10697R-2.IR_full 61 AATATACGCGAAAGGCGTGTTCTGCCGGAAGTGAAGCCTGGCTACCTGAAGCCACTGATT 120
||||||||||||||||| |||||||||||||||||||||||||||||||||||| |||||
10697R-2.in silico 61 AATATACGCGAAAGGCGCGTTCTGCCGGAAGTGAAGCCTGGCTACCTGAAGCCATTGATT 120
10697R-2.IR_full 121 CCGGATGCTGCGCCCGAGAAGCCGGAGAAGTGGCAGGATGTGATGCAGGACATCGAGCGA 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10697R-2.in silico 121 CCGGATGCTGCGCCCGAGAAGCCGGAGAAGTGGCAGGATGTGATGCAGGACATCGAGCGA 180
10697R-2.IR_full 181 GTCATCATGCCGGGCGTGACACACTGGCACAGTCCCAAGTTTCATGCCTACTTCCCCACG 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10697R-2.in silico 181 GTCATCATGCCGGGCGTGACACACTGGCACAGTCCCAAGTTTCATGCCTACTTCCCCACG 240
10697R-2.IR_full 241 GCCAACTCGTATCCAGCGATCGTTGCGGACATGCTGAGTGGAGCGATTGCCTGCATCGGA 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10697R-2.in silico 241 GCCAACTCGTATCCAGCGATCGTTGCGGACATGCTGAGTGGAGCGATTGCCTGCATCGGA 300
10697R-2.IR_full 301 TTCACGTGGATCGCCAGTCCCGCGTGCACGGAACTCGAGGTGGTCATGATGGATTGGCTG 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10697R-2.in silico 301 TTCACGTGGATCGCCAGTCCCGCGTGCACGGAACTCGAGGTGGTCATGATGGATTGGCTG 360
10697R-2.IR_full 361 GGCAAGATGCTGGAGCTGCCGGCAGAGTTCCTGGCCTGTTCGGGCGGCAAGGGTGGCGGT 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10697R-2.in silico 361 GGCAAGATGCTGGAGCTGCCGGCAGAGTTCCTGGCCTGTTCGGGCGGCAAGGGTGGCGGT 420
10697R-2.IR_full 421 GTCATCCAGGGCACGGCCAGTGAGTCCACACTGGTGGCTCTGCTGGGAGCCAAGGCCAAG 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10697R-2.in silico 421 GTCATCCAGGGCACGGCCAGTGAGTCCACACTGGTGGCTCTGCTGGGAGCCAAGGCCAAG 480
10697R-2.IR_full 481 AAGTTGAAGGAGGTGAAGGA 500
||||||||||||||||||||
10697R-2.in silico 481 AAGTTGAAGGAGGTGAAGGA 500
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_165280.1 |
CG10697-RB, transcript variant B (Ddc), mRNA |
| 100 |
482 |
0 |
0 |
0 |
NM_165279.1 |
CG10697-RC, transcript variant C (Ddc), mRNA |
| 100 |
482 |
0 |
0 |
0 |
NM_078876.4 |
CG10697-RA, transcript variant A (Ddc), mRNA |
| 0 |
0 |
6 |
2 |
4 |
NM_137994.2 |
CG5554-RA (CG5554), mRNA |
| 0 |
0 |
5 |
13 |
37 |
NM_057244.3 |
CG10501-RA, transcript variant A (amd), mRNA |
| 0 |
0 |
4 |
9 |
14 |
NM_165483.1 |
CG30446-RA (Tdc2), mRNA |
| 0 |
0 |
4 |
6 |
5 |
NM_176399.1 |
CG31531-RC, transcript variant C (CG31531), mRNA |
| 0 |
0 |
4 |
6 |
5 |
NM_169014.1 |
CG31531-RA, transcript variant A (CG31531), mRNA |
| 0 |
0 |
4 |
6 |
5 |
NM_169015.1 |
CG31531-RB, transcript variant B (CG31531), mRNA |
| 0 |
0 |
3 |
4 |
6 |
NM_136673.2 |
CG1698-RA (CG1698), mRNA |
| 0 |
0 |
3 |
2 |
2 |
NM_131940.1 |
CG15912-RA (CG15912), mRNA |
| 0 |
0 |
2 |
6 |
3 |
NM_057310.3 |
CG5637-RA (nos), mRNA |
| 0 |
0 |
2 |
6 |
3 |
NM_058005.3 |
CG9738-RA (Mkk4), mRNA |
| 0 |
0 |
2 |
6 |
2 |
NM_001042838.1 |
CG40500-RC, transcript variant C (CG40500), mRNA |
| 0 |
0 |
2 |
6 |
2 |
NM_001042841.1 |
CG40500-RD, transcript variant D (CG40500), mRNA |
| 0 |
0 |
2 |
6 |
2 |
NM_001042839.1 |
CG40500-RB, transcript variant B (CG40500), mRNA |
| 0 |
0 |
2 |
6 |
2 |
NM_001042840.1 |
CG40500-RA, transcript variant A (CG40500), mRNA |
| 0 |
0 |
2 |
3 |
5 |
NM_165541.1 |
CG30498-RA (boca), mRNA |
| 0 |
0 |
2 |
3 |
5 |
NM_001014563.1 |
CG7524-RF, transcript variant F (Src64B), mRNA |
| 0 |
0 |
2 |
3 |
5 |
NM_001014562.1 |
CG7524-RC, transcript variant C (Src64B), mRNA |
| 0 |
0 |
2 |
3 |
5 |
NM_001014561.1 |
CG7524-RD, transcript variant D (Src64B), mRNA |
| 0 |
0 |
2 |
3 |
5 |
NM_080195.2 |
CG7524-RB, transcript variant B (Src64B), mRNA |
| 0 |
0 |
2 |
3 |
5 |
NM_001014564.1 |
CG7524-RE, transcript variant E (Src64B), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_169030.1 |
CG2604-RC, transcript variant C (CG2604), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_169029.1 |
CG2604-RB, transcript variant B (CG2604), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_141260.2 |
CG2604-RA, transcript variant A (CG2604), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_137960.2 |
CG3957-RA (CG3957), mRNA |
| 0 |
0 |
2 |
2 |
6 |
NM_057865.3 |
CG3324-RA (Pkg21D), mRNA |
| 0 |
0 |
1 |
6 |
10 |
NM_166125.2 |
CG18255-RA, transcript variant A (Strn-Mlck), mRNA |
| 0 |
0 |
1 |
5 |
4 |
NM_078495.1 |
CG15779-RA (Tre), mRNA |
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