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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
discarded
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
10580R-2 |
| Symbol |
fng |
Full Name |
fringe |
| CG No |
CG10580 |
Old CG No |
CG10580 |
| Synonyms |
Dfng, CG10580, D-Fng, l(3)rG554, frg, fg, Frg, D-fng, fng, Fng |
| Accession No (Link to NCBI) |
|
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
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Reference
[Please submit your publication]
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Yamamoto-Hino M, Yoshida H, Ichimiya T, Sakamura S, Maeda M, Kimura Y, Sasaki N, Aoki-Kinoshita KF, Kinoshita-Toyoda A, Toyoda H, Ueda R, Nishihara S, Goto S.
Phenotype-based clustering of glycosylation-related genes by RNAi-mediated gene silencing.
Genes Cells (2015) 20(6) 521-42
[
PubMed ID = 25940448
]
[
RRC reference
]
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| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGCAAGCCATGATGTTAGCCGT |
| Primer Seq. 3' |
aatctagaggtaccTGAAGAACCAGGTCTGATCGC |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
|
| in silico PCR Fragment |
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| Assemble Data |
10580R-2.IR full 1 GCAAGCCATGATGTTAGCCGTCGCCGTCGTCTATATGACCCTACTGCTCTACCAGAGCGC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10580R-2.in silico 1 GCAAGCCATGATGTTAGCCGTCGCCGTCGTCTATATGACCCTACTGCTCTACCAGAGCGC 60
10580R-2.IR full 61 CTACGGATATCCGGGCATCCAAGTGCCGCACAGCCAGGTGGATGCCCTTGCCAGCGAAGC 120
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10580R-2.in silico 61 CTACGGATATCCGGGCATCCAAGTGCCGCACAGCCAGGTGGATGCCCTTGCCAGCGAAGC 120
10580R-2.IR full 121 CGTGACCACACATCGCGACCAGCTGCTCCAGGACTACGTGCAGTCCTCCACGCCCACCCA 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10580R-2.in silico 121 CGTGACCACACATCGCGACCAGCTGCTCCAGGACTACGTGCAGTCCTCCACGCCCACCCA 180
10580R-2.IR full 181 GCCGGGGGCTGGAGCTCCGGCCGCCTCCCCCACCACCGTCATAATCCGCAAGGATATACG 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10580R-2.in silico 181 GCCGGGGGCTGGAGCTCCGGCCGCCTCCCCCACCACCGTCATAATCCGCAAGGATATACG 240
10580R-2.IR full 241 CAGCTTCAATTTCAGCGACATCGAGGTCAGTGAGCGGCCCACGGCCACGCTGCTAACAGA 300
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10580R-2.in silico 241 CAGCTTCAATTTCAGCGACATCGAGGTCAGTGAGCGGCCCACGGCCACGCTGCTAACAGA 300
10580R-2.IR full 301 ATTGGCCAGAAGGAGCCGGAATGGGGAACTGCTCCGCGATCTGTCCCAAAGAGCGGTGAC 360
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10580R-2.in silico 301 ATTGGCCAGAAGGAGCCGGAATGGGGAACTGCTCCGCGATCTGTCCCAAAGAGCGGTGAC 360
10580R-2.IR full 361 GGCGACACCACAGCCGCCGGTCACCGAGTTGGATGACATTTTCATCAGCGTAAAGACGAC 420
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10580R-2.in silico 361 GGCGACACCACAGCCGCCGGTCACCGAGTTGGATGACATTTTCATCAGCGTAAAGACGAC 420
10580R-2.IR full 421 GAAGAACTATCACGACACCCGGCTGGCGTTGATCATCAAGACCTGGTTCCAATTGGCCCG 480
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10580R-2.in silico 421 GAAGAACTATCACGACACCCGGCTGGCGTTGATCATCAAGACCTGGTTCCAATTGGCCCG 480
10580R-2.IR full 481 CGATCAGACCTGGTTCTTCA 500
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10580R-2.in silico 481 CGATCAGACCTGGTTCTTCA 500
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
2 |
NM_079467.2 |
fringe CG10580-RA (fng), mRNA |
| 0 |
0 |
4 |
6 |
21 |
NM_132386.2 |
Interaction partner of Dnmt2 CG2961-RA (Ipod), mRNA |
| 0 |
0 |
3 |
3 |
10 |
NM_001042891.1 |
bruno-2 CG31761-RE, transcript variant E (bru-2), mRNA |
| 0 |
0 |
2 |
6 |
2 |
NM_079664.1 |
Salivary gland secretion 5 CG7596-RA (Sgs5), mRNA |
| 0 |
0 |
2 |
5 |
7 |
NM_136798.1 |
skiff CG30021-RA (skf), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_166978.1 |
CG13021-RC, transcript variant C (CG13021), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_166976.1 |
CG32791-RA (CG32791), mRNA |
| 0 |
0 |
1 |
5 |
4 |
NM_164797.1 |
CG8475-RB, transcript variant B (CG8475), mRNA |
| 0 |
0 |
1 |
5 |
4 |
NM_135345.2 |
CG8475-RA, transcript variant A (CG8475), mRNA |
| 0 |
0 |
1 |
5 |
3 |
NM_130621.2 |
CG4061-RA (CG4061), mRNA |
| 0 |
0 |
1 |
4 |
5 |
NM_001038810.1 |
porin CG6647-RC, transcript variant C (porin), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_135123.2 |
CG9021-RA (CG9021), mRNA |
| 0 |
0 |
1 |
3 |
6 |
NM_143770.2 |
lethal (2) 01424 CG3845-RB, transcript variant B (l(2)01424), mRNA |
| 0 |
0 |
1 |
3 |
6 |
NM_001038854.1 |
lethal (2) 01424 CG3845-RC, transcript variant C (l(2)01424), mRNA |
| 0 |
0 |
1 |
3 |
6 |
NM_165961.1 |
lethal (2) 01424 CG3845-RA, transcript variant A (l(2)01424), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_132773.2 |
rab3-GEF CG5627-RA (rab3-GEF), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_164989.1 |
rab3-GAP CG7061-RB, transcript variant B (rab3-GAP), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_135700.3 |
rab3-GAP CG7061-RA, transcript variant A (rab3-GAP), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_134876.1 |
CG18558-RA (CG18558), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_080378.2 |
UDP-GlcNAc:a-3-D-mannoside-beta-1,2-N-acetylglucosaminyltransferase I CG13431-RA (Mgat1), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_169858.1 |
CG5316-RC, transcript variant C (CG5316), mRNA |
| 0 |
0 |
1 |
2 |
9 |
NM_079817.2 |
Dynein heavy chain at 89D CG1842-RA (Dhc98D), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_169740.1 |
Daughters against dpp CG5201-RB, transcript variant B (Dad), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_143050.1 |
CG11069-RA (CG11069), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_001014629.1 |
Daughters against dpp CG5201-RC, transcript variant C (Dad), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_057912.3 |
Daughters against dpp CG5201-RA, transcript variant A (Dad), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_137672.1 |
CG15225-RA (CG15225), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_134701.2 |
CG3883-RA (CG3883), mRNA |
| 0 |
0 |
0 |
7 |
22 |
NM_078641.2 |
cabeza CG3606-RB, transcript variant B (caz), mRNA |
| 0 |
0 |
0 |
7 |
22 |
NM_167493.1 |
cabeza CG3606-RA, transcript variant A (caz), mRNA |
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