National Institute of Genetics
Contact us      Quick Search       
NIG-Fly - Fly Stocks of National Institute of Genetics (NIG) Drosophila strain -NIG-Fly - Fly Stocks of National Institute of Genetics (NIG) Drosophila strain -NIG-Fly - Fly Stocks of National Institute of Genetics (NIG) Drosophila strain -
Home Home
Japanese | English  
Notice:

Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
NIG-RNAi Strains Detail
Request : Click the "Order" button to request for this stock   request request_help.gif
Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 10562R-2 
 Symbol CG10562  Full Name CG10562 
 CG No CG10562  Old CG No CG10562 
 Synonyms CG10562 
 Accession No (Link to NCBI) NM_143128.2 
 Inserted Chr. ll 
 Insertional Mutation  lethal 
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgACATCACATTCGGCGCCAAAA 
 Primer Seq. 3' 
aatctagaggtaccCAGCTGGTAGTCGACCAAATA 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 ACATCACATT CGGCGCCAAA AACTATGATC TCAAGAATGC CAAGACAGAC TACGTGGCCC 
0061 TGGAGGATTT GGGGTTGAAA GGGTTCAAGA ATGCCAACCG CCTCGAAGGG CTGGATCAAG 
0121 AGCACACGGA GAGGGTTCTG CGAAAGCTAN TCCCAATGGC ATGCTGCATC AGCTGTACGA 
0181 GTGGCCACCA AAGGACCCTA TCCCAAAATC CTACTTCAAG GCTTCTTTAA GGAGGAAAGC 
0241 AGGCCCGTTA TGTCTGAAAT GATAAAAGGA ATGGGCGCGA ATTTCGTTAA AAGCTGCGCC 
0301 ACCTACGAAG GTCATGAGGC TTATTTAGAC AAAGTCAAAG CGTTACAGCC TGTTGCTATA 
0361 GATAAGATTT TTGAGTTCGC CAAGGTCGAG CCAACGGAGT TCAACGTTCT GAACCACGGC 
0421 GATTCGTGGT CAAACAATAT AATGTTTCAG TATGATGCCT TTGGAAAGAT CAAGGAGGTG 
0481 TATTTGGTCG ACTACCAGCT G 
 in silico PCR Fragment 
0001 ACATCACATT CGGCGCCAAA AACTATGATC TCAAGAATGC CAAGACAGAC TACGTGGCCC 
0061 TGGAGGATTT GGGGTTGAAA GGGTTCAAGA ATGCCAACCG CCTCGAAGGG CTGGATCAAG 
0121 AGCACACGGA GAGGGTTCTG CGAAAGCTA- TCCCAATGGC ATGCTGCATC AGCTGTACGA 
0181 GTGGCCACCA AAGGACCCTA TCCCAAAATC CTACTTCAAG GCTTCTTTAA GGAGGAAAGC 
0241 AGGCCCGTTA TGTCTGAAAT GATAAAAGGA ATGGGCGCGA ATTTCGTTAA AAGCTGCGCC 
0301 ACCTACGAAG GTCATGAGGC TTATTTAGAC AAAGTCAAAG CGTTACAGCC TGTTGCTATA 
0361 GATAAGATTT TTGAGTTCGC CAAGGTCGAG CCAACGGAGT TCAACGTTCT GAACCACGGC 
0421 GATTCGTGGT CAAACAATAT AATGTTTCAG TATGATGCCT TTGGAAAGAT CAAGGAGGTG 
0481 TATTTGGTCG ACTACCAGCT G 
 Assemble Data 

10562R-2.IR_full       1   ACATCACATTCGGCGCCAAAAACTATGATCTCAAGAATGCCAAGACAGACTACGTGGCCC 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10562R-2.in silico     1   ACATCACATTCGGCGCCAAAAACTATGATCTCAAGAATGCCAAGACAGACTACGTGGCCC 60


10562R-2.IR_full       61  TGGAGGATTTGGGGTTGAAAGGGTTCAAGAATGCCAACCGCCTCGAAGGGCTGGATCAAG 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10562R-2.in silico     61  TGGAGGATTTGGGGTTGAAAGGGTTCAAGAATGCCAACCGCCTCGAAGGGCTGGATCAAG 120


10562R-2.IR_full       121 AGCACACGGAGAGGGTTCTGCGAAAGCTANTCCCAATGGCATGCTGCATCAGCTGTACGA 180
                           ||||||||||||||||||||||||||||| ||||||||||||||||||||||||||||||
10562R-2.in silico     121 AGCACACGGAGAGGGTTCTGCGAAAGCTA-TCCCAATGGCATGCTGCATCAGCTGTACGA 180


10562R-2.IR_full       181 GTGGCCACCAAAGGACCCTATCCCAAAATCCTACTTCAAGGCTTCTTTAAGGAGGAAAGC 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10562R-2.in silico     181 GTGGCCACCAAAGGACCCTATCCCAAAATCCTACTTCAAGGCTTCTTTAAGGAGGAAAGC 240


10562R-2.IR_full       241 AGGCCCGTTATGTCTGAAATGATAAAAGGAATGGGCGCGAATTTCGTTAAAAGCTGCGCC 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10562R-2.in silico     241 AGGCCCGTTATGTCTGAAATGATAAAAGGAATGGGCGCGAATTTCGTTAAAAGCTGCGCC 300


10562R-2.IR_full       301 ACCTACGAAGGTCATGAGGCTTATTTAGACAAAGTCAAAGCGTTACAGCCTGTTGCTATA 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10562R-2.in silico     301 ACCTACGAAGGTCATGAGGCTTATTTAGACAAAGTCAAAGCGTTACAGCCTGTTGCTATA 360


10562R-2.IR_full       361 GATAAGATTTTTGAGTTCGCCAAGGTCGAGCCAACGGAGTTCAACGTTCTGAACCACGGC 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10562R-2.in silico     361 GATAAGATTTTTGAGTTCGCCAAGGTCGAGCCAACGGAGTTCAACGTTCTGAACCACGGC 420


10562R-2.IR_full       421 GATTCGTGGTCAAACAATATAATGTTTCAGTATGATGCCTTTGGAAAGATCAAGGAGGTG 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10562R-2.in silico     421 GATTCGTGGTCAAACAATATAATGTTTCAGTATGATGCCTTTGGAAAGATCAAGGAGGTG 480


10562R-2.IR_full       481 TATTTGGTCGACTACCAGCTG 501
                           |||||||||||||||||||||
10562R-2.in silico     481 TATTTGGTCGACTACCAGCTG 501



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_143128.2  CG10562-RA (CG10562), mRNA 
0   0  10  54  85  NM_170222.1  CG10618-RA (CHKov1), mRNA 
0   0  2  5  12  NM_141847.2  CG6834-RA (CG6834), mRNA 
0   0  2  3  4  NM_135412.1  CG9487-RA (CG9487), mRNA 
0   0  2  3  2  NM_140295.2  CG6811-RA (RhoGAP68F), mRNA 
0   0  2  2  4  NM_143030.2  CG18410-RA (CG18410), mRNA 
0   0  2  1  4  NM_079817.2  CG1842-RA (Dhc98D), mRNA 
0   0  1  4  4  NM_169061.1  CG1842-RA (Dhc98D), mRNA,4,5,-tris-phosphate receptor CG1063-RA, transcript variant A (Itp-r83A), mRNA 
0   0  1  4  4  NM_169060.1  CG1842-RA (Dhc98D), mRNA,4,5,-tris-phosphate receptor CG1063-RA, transcript variant A (Itp-r83A), mRNA,4,5,-tris-phosphate receptor CG1063-RB, transcript variant B (Itp-r83A), mRNA 
0   0  1  4  2  NM_142142.1  CG7292-RA (Rrp6), mRNA 
0   0  1  3  4  NM_168129.1  CG5486-RA, transcript variant A (Ubp64E), mRNA 
0   0  1  3  4  NM_206279.1  CG5486-RC, transcript variant C (Ubp64E), mRNA 
0   0  1  3  4  NM_079213.2  CG5486-RB, transcript variant B (Ubp64E), mRNA 
0   0  1  2  5  NM_057666.3  CG10922-RA, transcript variant A (La), mRNA 
0   0  1  2  5  NM_165326.1  CG10922-RB, transcript variant B (La), mRNA 
0   0  1  2  3  NM_138031.1  CG3173-RA (CG3173), mRNA 
0   0  1  2  2  NM_205899.1  CG33282-RA (CG33282), mRNA 
0   0  0  9  11  NM_170219.1  CG10550-RC, transcript variant C (CG10550), mRNA 
0   0  0  9  11  NM_170218.1  CG10550-RB, transcript variant B (CG10550), mRNA 
0   0  0  9  11  NM_143123.2  CG10550-RA, transcript variant A (CG10550), mRNA 
0   0  0  5  6  NM_136621.2  CG8046-RB, transcript variant B (CG8046), mRNA 
0   0  0  5  6  NM_165657.1  CG8046-RA, transcript variant A (CG8046), mRNA 
0   0  0  5  2  NM_134605.2  CG1696-RA (l(1)G0269), mRNA 
0   0  0  4  7  NM_165086.1  CG15288-RB, transcript variant B (wb), mRNA 
0   0  0  4  7  NM_057442.3  CG15288-RA, transcript variant A (wb), mRNA 
0   0  0  4  6  NM_132890.2  CG3692-RA (CalpC), mRNA 
0   0  0  4  6  NM_080102.3  CG10506-RA (Aats-gln), mRNA 
0   0  0  4  4  NM_169089.2  CG1250-RB, transcript variant B (sec23), mRNA 
0   0  0  4  4  NM_169088.1  CG1250-RA, transcript variant A (sec23), mRNA 
0   0  0  4  3  NM_134821.1  CG4271-RA (CG4271), mRNA 

SHIGEN - SHared Information of GENetic resources - National Institute of Genetics (NIG)
 Copyright (c) National Institute of Genetics (NIG)
All rights reserved.
The NIG-Fly is a part of the National BioResource Project which started in 2002.