NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 10532R-2

Symbol CG32944 Full Name CG32944

CG No CG32944 Old CG No CG10532

Synonyms CG9818, CG10532, CG32944

Accession No (Link to NCBI) NM_176397.2

Inserted Chr. ll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees viable

Map Viewer

Reference
[Please submit your publication] Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.
Zyxin links fat signaling to the hippo pathway.
PLoS Biol (2011) 9(6) e1000624 [ PubMed ID = 21666802 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGCACTCGAACACCCCACTAGC

Primer Seq. 3'

aatctagaggtaccTCTCTTGGATGGGGGCCAAGC

Predicted Fragment Size

IR fragment full Seq

0001 GCACTCGAAC ACCCCACTAG CGGAGATCAA GAATATATTG AACACCCCCG TGCACTATCC 
0061 GCGCTACTGG AGCAGCAACT TCGTCGATCT GCTGCAGAGG TTACTTTCCA CCTATCCGGG 
0121 TGCTAGGATC AGTACAAGGC AGGAGCTGCA TCAAACGCCC ATGTTGCGCA ACATTGACTT 
0181 CCAGAGGGTG CTCGAGAAGA AGATCAAACC CATCTATAAA CCTCCCGAAG ACCACCTCAA 
0241 CTGTGATCCT TGCCTAGAAC TGGAGGAAAT GATTGTAGAA TCGCGGCCCT TGCATAAAAA 
0301 GAAGAAGCGC CTGGCCAAGC AGAGGTCAGC ACAACGCGAC AGCGATCCAG AAGGACTGCA 
0361 CTGGTCAAAG AGTTCATCGT TTACAACCGC TACAAGGAGT TGAAGCGNAA AGCCATGGAA 
0421 CAAAAGGAAA ACGACTGGCA GCGCGAACTG GNAACTGGCC ATGGCCAACT CCATCGTGAA 
0481 CAGCTTGGCC CCCATCCAAG AGA

in silico PCR Fragment

0001 GCACTCGAAC ACCCCACTAG CGGAGATCAA GAATATATTG AACACCCCCG TGCACTATCC 
0061 GCGCTACTGG AGCAGCAACT TCGTCGATCT GCTGCAGAGG TTACTTTCCA CCTATCCGGG 
0121 TGCTAGGATC AGTACAAGGC AGGAGCTGCA TCAAACGCCC ATGTTGCGCA ACATTGACTT 
0181 CCAGAGGGTG CTCGAGAAGA AGATCAAACC CATCTATAAA CCTCCCGAAG ACCACCTCAA 
0241 CTGTGATCCT TGCCTAGAAC TGGAGGAAAT GATTGTAGAA TCGCGGCCCT TGCATAAAAA 
0301 GAAGAAGCGC CTGGCCAAGC AGAGGTCAGC ACAACGCGAC AGCGATCCAG A--GACTGCA 
0361 CTGGTCAAAG AGTTCATCGT TTACAACCGC TACAAGGAGT TGAAGCGCAA AGCCATGGAA 
0421 CAAAAGGAAA ACGACTGGCA GCGCGAACTG G-AACTGGCC ATGGCCAACT CCATCGTGAA 
0481 CAGCTTGGCC CCCATCCAAG AGA

Assemble Data


10532R-2.IR_full       1   GCACTCGAACACCCCACTAGCGGAGATCAAGAATATATTGAACACCCCCGTGCACTATCC 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10532R-2.in silico     1   GCACTCGAACACCCCACTAGCGGAGATCAAGAATATATTGAACACCCCCGTGCACTATCC 60


10532R-2.IR_full       61  GCGCTACTGGAGCAGCAACTTCGTCGATCTGCTGCAGAGGTTACTTTCCACCTATCCGGG 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10532R-2.in silico     61  GCGCTACTGGAGCAGCAACTTCGTCGATCTGCTGCAGAGGTTACTTTCCACCTATCCGGG 120


10532R-2.IR_full       121 TGCTAGGATCAGTACAAGGCAGGAGCTGCATCAAACGCCCATGTTGCGCAACATTGACTT 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10532R-2.in silico     121 TGCTAGGATCAGTACAAGGCAGGAGCTGCATCAAACGCCCATGTTGCGCAACATTGACTT 180


10532R-2.IR_full       181 CCAGAGGGTGCTCGAGAAGAAGATCAAACCCATCTATAAACCTCCCGAAGACCACCTCAA 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10532R-2.in silico     181 CCAGAGGGTGCTCGAGAAGAAGATCAAACCCATCTATAAACCTCCCGAAGACCACCTCAA 240


10532R-2.IR_full       241 CTGTGATCCTTGCCTAGAACTGGAGGAAATGATTGTAGAATCGCGGCCCTTGCATAAAAA 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10532R-2.in silico     241 CTGTGATCCTTGCCTAGAACTGGAGGAAATGATTGTAGAATCGCGGCCCTTGCATAAAAA 300


10532R-2.IR_full       301 GAAGAAGCGCCTGGCCAAGCAGAGGTCAGCACAACGCGACAGCGATCCAGAAGGACTGCA 360
                           |||||||||||||||||||||||||||||||||||||||||||||||||||  |||||||
10532R-2.in silico     301 GAAGAAGCGCCTGGCCAAGCAGAGGTCAGCACAACGCGACAGCGATCCAGA--GACTGCA 360


10532R-2.IR_full       361 CTGGTCAAAGAGTTCATCGTTTACAACCGCTACAAGGAGTTGAAGCGNAAAGCCATGGAA 420
                           ||||||||||||||||||||||||||||||||||||||||||||||| ||||||||||||
10532R-2.in silico     361 CTGGTCAAAGAGTTCATCGTTTACAACCGCTACAAGGAGTTGAAGCGCAAAGCCATGGAA 420


10532R-2.IR_full       421 CAAAAGGAAAACGACTGGCAGCGCGAACTGGNAACTGGCCATGGCCAACTCCATCGTGAA 480
                           ||||||||||||||||||||||||||||||| ||||||||||||||||||||||||||||
10532R-2.in silico     421 CAAAAGGAAAACGACTGGCAGCGCGAACTGG-AACTGGCCATGGCCAACTCCATCGTGAA 480


10532R-2.IR_full       481 CAGCTTGGCCCCCATCCAAGAGA 503
                           |||||||||||||||||||||||
10532R-2.in silico     481 CAGCTTGGCCCCCATCCAAGAGA 503

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	12	NM_001043201.1	CG32944-RC, transcript variant C (CG32944), mRNA
100	482	0	0	12	NM_176397.2	CG32944-RB, transcript variant B (CG32944), mRNA
0.2	1	2	2	3	NM_079859.2	CG1438-RA (Cyp4c3), mRNA
0	0	7	4	2	NM_132642.2	CG15744-RA (CG15744), mRNA
0	0	4	3	6	NM_132317.1	CG12121-RA (CG12121), mRNA
0	0	3	2	6	NM_136755.2	CG16728-RA (CG16728), mRNA
0	0	3	2	6	NM_078740.2	CG8817-RB, transcript variant B (lilli), mRNA
0	0	3	2	6	NM_164516.1	CG8817-RA, transcript variant A (lilli), mRNA
0	0	3	2	6	NM_164517.1	CG8817-RC, transcript variant C (lilli), mRNA
0	0	2	3	5	NM_141291.2	CG10018-RA (Snm1), mRNA
0	0	2	3	2	NM_141226.2	CG1057-RA, transcript variant A (MED31), mRNA
0	0	2	3	2	NM_169013.1	CG1057-RB, transcript variant B (MED31), mRNA
0	0	2	2	4	NM_142774.2	CG18596-RA (CG18596), mRNA
0	0	2	2	2	NM_135755.1	CG9932-RA (CG9932), mRNA
0	0	1	9	4	NM_078592.2	CG11172-RA (NFAT), mRNA
0	0	1	4	6	NM_135824.2	CG9014-RA (CG9014), mRNA
0	0	1	3	6	NM_078672.3	CG7113-RA (scu), mRNA
0	0	1	3	4	NM_166823.1	CG11059-RB, transcript variant B (cals), mRNA
0	0	1	3	4	NM_079893.2	CG11059-RA, transcript variant A (cals), mRNA
0	0	1	3	4	NM_168470.1	CG32085-RA (CG32085), mRNA
0	0	1	3	3	NM_168781.1	CG3961-RA, transcript variant A (CG3961), mRNA
0	0	1	3	3	NM_140810.2	CG3961-RC, transcript variant C (CG3961), mRNA
0	0	1	3	3	NM_168782.1	CG3961-RB, transcript variant B (CG3961), mRNA
0	0	1	2	3	NM_079823.2	CG1395-RA (stg), mRNA
0	0	1	2	3	NM_079992.3	CG17976-RA (sut3), mRNA
0	0	0	7	4	NM_142954.3	CG6057-RA (SMC1), mRNA
0	0	0	7	2	NM_137978.1	CG18128-RA (CG18128), mRNA
0	0	0	6	3	NM_136402.2	CG9460-RA (CG9460), mRNA
0	0	0	6	2	NM_079059.2	CG5519-RA (Gbp), mRNA
0	0	0	5	7	NM_132420.2	CG32676-RA (CG32676), mRNA

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