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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
10531R-1 |
| Symbol |
CG10531 |
Full Name |
CG10531 |
| CG No |
CG10531 |
Old CG No |
CG10531 |
| Synonyms |
CG10531 |
| Accession No (Link to NCBI) |
NM_137699.3 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Álvarez-Fernández C, Tamirisa S, Prada F, Chernomoretz A, Podhajcer O, Blanco E, Martín-Blanco E.
Identification and functional analysis of healing regulators in Drosophila.
PLoS Genet (2015) 11(2) e1004965
[
PubMed ID = 25647511
]
[
RRC reference
]
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgGTTATTGGCACTACTCGCTGT |
| Primer Seq. 3' |
aatctagaggtaccCGAACGCCTCCTTAATCTCGC |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 GTTATGCGNC TGGCACTACT CGCTGTCCTC TGCCTTAGCC AGGTTATCGG TGCTGAACGC
0061 ATCGTCAACT GCTACTGGGG CACCTGGGCC AACTACCGCA GCGGCAATGG CAAGTTCGAT
0121 GTCTCCAATA TCGATGCCGG TCTGTGCACC CATCTGAGCT ACTCCTTCTT CGGAATCAAC
0181 GACAATGGTG AGATCCAATC CCTGGACACT TGGCTGGACT ACGATCTGGG CTTCATCAAC
0241 CAGGCGATTA GCCTGANAGN AACCAAAACT CCAACCTGAA AGTCCTGGCC GTGGTCGGCG
0301 GCNTGGAACG AGGGCTCCAC CAAGTACTCC TCGATGTCCG GCGATTGGTA CAAGCGCCAG
0361 AACTTCATTA ACTCCGCCCT GAACCTGCTG CGCAACCATG GATTCGATGG TCTGGATCTG
0421 GACTGGGAGT ACCCCAACCA GCGTGGTGGC AACTGGAACG ATCGTGCCAA CTTTGTGANT
0481 CNGCTGCGCG AGATTAAGGA GGCGTTCg |
| in silico PCR Fragment |
0001 GTTAT----- TGGCACTACT CGCTGTCCTC TGCCTTAGCC AGGTTATCGG TGCTGAACGC
0061 ATCGTCAACT GCTACTGGGG CACCTGGGCC AACTACCGCA GCGGCAATGG CAAGTTCGAT
0121 GTGTCCAATA TCGATGCCGG TCTGTGCACC CATCTGAGCT ACTCCTTCTT CGGAATCAAC
0181 GACAATGGTG AGATCCAATC CCTGGACACT TGGCTGGACT ACGATCTGGG CTTCATCAAC
0241 CAGGCGATTA GCCTGA-AG- AACCAAAACT CCAACCTGAA AGTCCTGGCC GTGGTCGGCG
0301 GC-TGGAACG AGGGCTCCAC CAAGTACTCC TCGATGTCCG GCGATTGGTA CAAGCGCCAG
0361 AACTTCATTA ACTCCGCCCT GAACCTGCTG CGCAACCATG GATTCGATGG TCTGGATCTG
0421 GACTGGGAGT ACCCCAACCA GCGTGGTGGC AACTGGAACG ATCGTGCCAA CTTTGTGACT
0481 CTGCTGCGCG AGATTAAGGA GGCGTTCG |
| Assemble Data |
10531R-1.IR_full 1 GTTATGCGNCTGGCACTACTCGCTGTCCTCTGCCTTAGCCAGGTTATCGGTGCTGAACGC 60
||||| ||||||||||||||||||||||||||||||||||||||||||||||||||
10531R-1.in silico 1 GTTAT-----TGGCACTACTCGCTGTCCTCTGCCTTAGCCAGGTTATCGGTGCTGAACGC 60
10531R-1.IR_full 61 ATCGTCAACTGCTACTGGGGCACCTGGGCCAACTACCGCAGCGGCAATGGCAAGTTCGAT 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10531R-1.in silico 61 ATCGTCAACTGCTACTGGGGCACCTGGGCCAACTACCGCAGCGGCAATGGCAAGTTCGAT 120
10531R-1.IR_full 121 GTCTCCAATATCGATGCCGGTCTGTGCACCCATCTGAGCTACTCCTTCTTCGGAATCAAC 180
|| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10531R-1.in silico 121 GTGTCCAATATCGATGCCGGTCTGTGCACCCATCTGAGCTACTCCTTCTTCGGAATCAAC 180
10531R-1.IR_full 181 GACAATGGTGAGATCCAATCCCTGGACACTTGGCTGGACTACGATCTGGGCTTCATCAAC 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10531R-1.in silico 181 GACAATGGTGAGATCCAATCCCTGGACACTTGGCTGGACTACGATCTGGGCTTCATCAAC 240
10531R-1.IR_full 241 CAGGCGATTAGCCTGANAGNAACCAAAACTCCAACCTGAAAGTCCTGGCCGTGGTCGGCG 300
|||||||||||||||| || ||||||||||||||||||||||||||||||||||||||||
10531R-1.in silico 241 CAGGCGATTAGCCTGA-AG-AACCAAAACTCCAACCTGAAAGTCCTGGCCGTGGTCGGCG 300
10531R-1.IR_full 301 GCNTGGAACGAGGGCTCCACCAAGTACTCCTCGATGTCCGGCGATTGGTACAAGCGCCAG 360
|| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10531R-1.in silico 301 GC-TGGAACGAGGGCTCCACCAAGTACTCCTCGATGTCCGGCGATTGGTACAAGCGCCAG 360
10531R-1.IR_full 361 AACTTCATTAACTCCGCCCTGAACCTGCTGCGCAACCATGGATTCGATGGTCTGGATCTG 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10531R-1.in silico 361 AACTTCATTAACTCCGCCCTGAACCTGCTGCGCAACCATGGATTCGATGGTCTGGATCTG 420
10531R-1.IR_full 421 GACTGGGAGTACCCCAACCAGCGTGGTGGCAACTGGAACGATCGTGCCAACTTTGTGANT 480
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||| |
10531R-1.in silico 421 GACTGGGAGTACCCCAACCAGCGTGGTGGCAACTGGAACGATCGTGCCAACTTTGTGACT 480
10531R-1.IR_full 481 CNGCTGCGCGAGATTAAGGAGGCGTTCG 508
| ||||||||||||||||||||||||||
10531R-1.in silico 481 CTGCTGCGCGAGATTAAGGAGGCGTTCG 508
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_137699.3 |
CG10531-RA (CG10531), mRNA |
| 1.24 |
6 |
26 |
20 |
31 |
NM_080223.2 |
CG3986-RA (Cht4), mRNA |
| 0.41 |
2 |
4 |
3 |
2 |
NM_137279.3 |
CG6262-RA, transcript variant A (CG6262), mRNA |
| 0.41 |
2 |
4 |
3 |
2 |
NM_166172.3 |
CG6262-RB, transcript variant B (CG6262), mRNA |
| 0.2 |
1 |
6 |
10 |
19 |
NM_057950.2 |
CG2054-RA (Cht2), mRNA |
| 0 |
0 |
5 |
15 |
23 |
NM_137698.1 |
CG9357-RA (CG9357), mRNA |
| 0 |
0 |
2 |
12 |
16 |
NM_139511.2 |
CG1869-RA (CG1869), mRNA |
| 0 |
0 |
2 |
6 |
3 |
NM_057545.3 |
CG14513-RA (yemalpha), mRNA |
| 0 |
0 |
2 |
4 |
4 |
NM_079198.2 |
CG15010-RC, transcript variant C (ago), mRNA |
| 0 |
0 |
2 |
4 |
4 |
NM_168073.1 |
CG15010-RB, transcript variant B (ago), mRNA |
| 0 |
0 |
2 |
4 |
4 |
NM_168072.1 |
CG15010-RA, transcript variant A (ago), mRNA |
| 0 |
0 |
2 |
4 |
3 |
NM_001042804.1 |
CG2174-RB, transcript variant B (Myo10A), mRNA |
| 0 |
0 |
2 |
4 |
2 |
NM_132441.1 |
CG2174-RA, transcript variant A (Myo10A), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_135656.2 |
CG4851-RA (CG4851), mRNA |
| 0 |
0 |
1 |
9 |
5 |
NM_139664.1 |
CG13715-RA (CG13715), mRNA |
| 0 |
0 |
1 |
3 |
6 |
NM_165794.2 |
CG11895-RA (stan), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_141003.2 |
CG3634-RA (CG3634), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_137538.1 |
CG15100-RA (CG15100), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_136900.1 |
CG8859-RA (Cyp6g2), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_132069.2 |
CG32754-RA (vanin-like), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_165158.1 |
CG4559-RC, transcript variant C (Idgf3), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_057908.3 |
CG4559-RA, transcript variant A (Idgf3), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_165157.1 |
CG4559-RB, transcript variant B (Idgf3), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_142731.1 |
CG6569-RA, transcript variant A (CG6569), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_169975.1 |
CG6569-RB, transcript variant B (CG6569), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_143601.2 |
CG12114-RA (CG12114), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_176195.1 |
CG33017-RB (CG33017), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_001043085.1 |
CG34123-RC, transcript variant C (CG34123), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_001043083.1 |
CG34123-RB, transcript variant B (CG34123), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_001043084.1 |
CG34123-RD, transcript variant D (CG34123), mRNA |
|
| Wing Disc |
GFP
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Puc
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Caspase
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DAPI
 |
Merge
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Figure
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JNK signal
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Caspase-3 signal
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