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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 10518R-1 
 Symbol CG30389  Full Name CG30389 
 CG No CG30389  Old CG No CG10518 
 Synonyms CG4126, CG10518, BcDNA:AT02070, CG30389 
 Accession No (Link to NCBI) NM_137711.3 
 Inserted Chr. ll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Ohhara Y, Hoshino G, Imahori K, Matsuyuki T, Yamakawa-Kobayashi K.
The Nutrient-Responsive Molecular Chaperone Hsp90 Supports Growth and Development in Drosophila.
Front Physiol (2021) 12 690564 [ PubMed ID = 34239451 ] [ RRC reference ]

Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.
Zyxin links fat signaling to the hippo pathway.
PLoS Biol (2011) 9(6) e1000624 [ PubMed ID = 21666802 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgGGCCATTCTGGCTGCTCCTTC 
 Primer Seq. 3' 
aatctagaggtaccAACGGTGCTGGATGTGGCTGC 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 GGCCATTCTG GCTGCTCCTT CGCTCCGTGC ACGATTCCTT CAAGTACAAG GGACTGGCAT 
0061 TCTCCGTACT GTTCGTGTGC ATAGCGATAA CGTCGGATCT GGTCTGTTTA TTCTTCATAC 
0121 CCGTTCACTG GCTGCTCTTT GCAGCGAGCA CTTATGTGTG GGTGCAGTAC GTCTGGCACA 
0181 CAGATAAGGG TATCTGTTTG CCGACTATAA TACTCTGGAT GTTATTTGTC TACCTGGAGG 
0241 TGGGCATAAG ATGGAAGGAC AGTCGCCACA TGCCGCACTT GGATCTCTGC CGGCCGTTCG 
0301 CAGCGCATTG CATTGGCTAT CCGGTTGTCA CGCTTGGATT TGGCTTTAAA AGCTACGTGG 
0361 GCTACCGCAT GCGCCAgcGC AAGCAGCGCG AGNGTAGCCA AGNGACAACG AGTTCTATAT 
0421 GCAGCTTCTG CAGCAgGNTC TGCCCGCGGA GGAGGCGGCC GAGGAGGCGG CTGCGGGGCA 
0481 GGCAGCCACA TCCAGCAcCG TT 
 in silico PCR Fragment 
0001 GGCCATTCTG GCTGCTCCTT CGCTCCGTGC ACGATTCCTT CAAGTACAAG GGACTGGCAT 
0061 TCTCCGTACT GTTCGTGTGC ATAGCGATAA CGTCGGATCT GGTCTGTTTA TTCTTCATAC 
0121 CCGTTCACTG GCTGCTCTTT GCAGCGAGCA CTTATGTGTG GGTGCAGTAC GTCTGGCACA 
0181 CAGATAAGGG TATCTGTTTG CCGACTATAA TACTCTGGAT GTTATTTGTC TACCTGGAGG 
0241 TGGGCATAAG ATGGAAGGAC AGTCGCCACA TGCCGCACTT GGATCTCTGC CGGCCGTTCG 
0301 CAGCGCATTG CATTGGCTAT CCGGTTGTCA CGCTTGGATT TGGCTTTAAA AGCTACGTGG 
0361 GCTACCGCAT GCGCCAGCGC AAGCAGCGCG AG-GTAGCCA AG-GACAACG AGTTCTATAT 
0421 GCAGCTTCTG CAGCAGGCTC TGCCCGCGGA GGAGGCGGCC GAGGAGGCGG CTGCGGGGCA 
0481 GGCAGCCACA TCCAGCACCG TT 
 Assemble Data 

10518R-1.IR_full       1   GGCCATTCTGGCTGCTCCTTCGCTCCGTGCACGATTCCTTCAAGTACAAGGGACTGGCAT 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10518R-1.in silico     1   GGCCATTCTGGCTGCTCCTTCGCTCCGTGCACGATTCCTTCAAGTACAAGGGACTGGCAT 60


10518R-1.IR_full       61  TCTCCGTACTGTTCGTGTGCATAGCGATAACGTCGGATCTGGTCTGTTTATTCTTCATAC 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10518R-1.in silico     61  TCTCCGTACTGTTCGTGTGCATAGCGATAACGTCGGATCTGGTCTGTTTATTCTTCATAC 120


10518R-1.IR_full       121 CCGTTCACTGGCTGCTCTTTGCAGCGAGCACTTATGTGTGGGTGCAGTACGTCTGGCACA 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10518R-1.in silico     121 CCGTTCACTGGCTGCTCTTTGCAGCGAGCACTTATGTGTGGGTGCAGTACGTCTGGCACA 180


10518R-1.IR_full       181 CAGATAAGGGTATCTGTTTGCCGACTATAATACTCTGGATGTTATTTGTCTACCTGGAGG 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10518R-1.in silico     181 CAGATAAGGGTATCTGTTTGCCGACTATAATACTCTGGATGTTATTTGTCTACCTGGAGG 240


10518R-1.IR_full       241 TGGGCATAAGATGGAAGGACAGTCGCCACATGCCGCACTTGGATCTCTGCCGGCCGTTCG 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10518R-1.in silico     241 TGGGCATAAGATGGAAGGACAGTCGCCACATGCCGCACTTGGATCTCTGCCGGCCGTTCG 300


10518R-1.IR_full       301 CAGCGCATTGCATTGGCTATCCGGTTGTCACGCTTGGATTTGGCTTTAAAAGCTACGTGG 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10518R-1.in silico     301 CAGCGCATTGCATTGGCTATCCGGTTGTCACGCTTGGATTTGGCTTTAAAAGCTACGTGG 360


10518R-1.IR_full       361 GCTACCGCATGCGCCAGCGCAAGCAGCGCGAGNGTAGCCAAGNGACAACGAGTTCTATAT 420
                           |||||||||||||||||||||||||||||||| ||||||||| |||||||||||||||||
10518R-1.in silico     361 GCTACCGCATGCGCCAGCGCAAGCAGCGCGAG-GTAGCCAAG-GACAACGAGTTCTATAT 420


10518R-1.IR_full       421 GCAGCTTCTGCAGCAGGNTCTGCCCGCGGAGGAGGCGGCCGAGGAGGCGGCTGCGGGGCA 480
                           ||||||||||||||||| ||||||||||||||||||||||||||||||||||||||||||
10518R-1.in silico     421 GCAGCTTCTGCAGCAGGCTCTGCCCGCGGAGGAGGCGGCCGAGGAGGCGGCTGCGGGGCA 480


10518R-1.IR_full       481 GGCAGCCACATCCAGCACCGTT 502
                           ||||||||||||||||||||||
10518R-1.in silico     481 GGCAGCCACATCCAGCACCGTT 502



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  5  NM_137711.3  CG30389-RA, transcript variant A (CG30389), mRNA 
100   482  0  0  5  NM_137712.3  CG30389-RC, transcript variant C (CG30389), mRNA 
17.63   85  1  1  4  NM_166428.2  CG30389-RB, transcript variant B (CG30389), mRNA 
0   0  8  2  4  NM_134718.4  CG31795-RA, transcript variant A (ia2), mRNA 
0   0  8  2  4  NM_164401.3  CG31795-RB, transcript variant B (ia2), mRNA 
0   0  3  4  6  NM_138097.2  CG3548-RA (CG3548), mRNA 
0   0  3  3  2  NM_080170.2  CG11156-RA (mus101), mRNA 
0   0  3  2  2  NM_057716.3  CG8705-RA, transcript variant A (pnut), mRNA 
0   0  3  2  2  NM_165597.1  CG8705-RB, transcript variant B (pnut), mRNA 
0   0  2  7  14  NM_132734.2  CG9411-RA (CG9411), mRNA 
0   0  2  5  12  NM_139725.2  CG5249-RA (Blimp-1), mRNA 
0   0  2  5  10  NM_168576.1  CG32138-RB, transcript variant B (CG32138), mRNA 
0   0  2  5  10  NM_168575.1  CG32138-RA, transcript variant A (CG32138), mRNA 
0   0  2  4  6  NM_136733.2  CG12907-RA (CG12907), mRNA 
0   0  2  4  2  NM_136882.2  CG30040-RA (jeb), mRNA 
0   0  2  3  15  NM_176556.1  CG33106-RA, transcript variant A (mask), mRNA 
0   0  2  3  15  NM_176557.1  CG33106-RB, transcript variant B (mask), mRNA 
0   0  2  2  14  NM_078717.2  CG3696-RA, transcript variant A (kis), mRNA 
0   0  2  2  14  NM_141374.1  CG1154-RA (Osi12), mRNA 
0   0  2  2  10  NM_165943.1  CG8772-RB, transcript variant B (nemy), mRNA 
0   0  2  2  4  NM_138194.2  CG17090-RA, transcript variant A (CG17090), mRNA 
0   0  2  2  4  NM_167834.1  CG17090-RB, transcript variant B (CG17090), mRNA 
0   0  2  2  4  NM_169698.1  CG5000-RA (msps), mRNA 
0   0  2  2  3  NM_143789.2  CG11899-RA (CG11899), mRNA 
0   0  1  7  6  NM_001038743.1  CG33980-RA (CG33980), mRNA 
0   0  1  5  4  NM_140842.1  CG9629-RA (CG9629), mRNA 
0   0  1  5  4  NM_175962.1  CG3294-RB, transcript variant B (CG3294), mRNA 
0   0  1  5  4  NM_135013.2  CG3294-RA, transcript variant A (CG3294), mRNA 
0   0  1  4  12  NM_141816.3  CG6764-RA (CG6764), mRNA 
0   0  1  4  10  NM_133114.2  CG32541-RA (CG32541), mRNA 

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