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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
10484R-3 |
| Symbol |
Dox-A2 |
Full Name |
Diphenol oxidase A2 |
| CG No |
CG10484 |
Old CG No |
CG10484 |
| Synonyms |
PO, p58, proPO, DmePPO2, Dox, DXA2_DROME, DoxA, DPO, l(2)37Bf, l(2)37Bf[+], DoxA2, CG10484, Dox-A2, PPO, ProPO |
| Accession No (Link to NCBI) |
NM_057952.3 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
larval lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Cho Y, Lai CM, Lin KY, Hsu HJ.
A Targeted RNAi Screen Reveals Drosophila Female-Sterile Genes That Control the Size of Germline Stem Cell Niche During Development.
G3 (Bethesda) (2018) 8(7) 2345-2354
[
PubMed ID = 29764959
]
[
RRC reference
]
Saj A, Arziman Z, Stempfle D, van Belle W, Sauder U, Horn T, Dürrenberger M, Paro R, Boutros M, Merdes G.
A combined ex vivo and in vivo RNAi screen for notch regulators in Drosophila reveals an extensive notch interaction network.
Dev Cell (2010) 18(5) 862-76
[
PubMed ID = 20493818
]
[
RRC reference
]
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| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgTGACCAACGCAACGGACATCG |
| Primer Seq. 3' |
aatctagaggtaccAATCAGATCAAGGGTGCGTCG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 TGACCAACGC AACGGACATC GGTGCTAACG ACGTGGAGAT GGAGGTGGAT CCAACGGCGG
0061 AGACGCTGGC TGACGAGAAG AAGAACCAAG ATGTGGCCGC CGTGCAGGAG ATCCGCGAGC
0121 AGATTCGTCA GATTGAGAAG GGGGTAGCCT CGAAAGAGTC GCGCTTCATC CTGCGCGTCC
0181 TTCGCAATTT GCCCAACACT CGTCGCAAGC TGAACGGCGT CGTCTTCCGG AATCTTGCAC
0241 AGAGTATTTA CCCCGCTGGT GCAGATCGTG AGGCGGCCGT GGCTTTGATG CCCGCTGTGG
0301 AGAAAGACGC CACCGAGCTG CCCGATGTTC CCAAAAAACA AGTTGCCACC AAGGCTCCAA
0361 TCGCCGAGGT CGATGCCTAC TTCTACCTGC TCCTGCTGGT CAAGCTCATC GACGCCAGTG
0421 ATTTAAAGCG GGCCGGAATT AGCGCCGACG CCCTAATGGC CAAAATCTCC ATCCAAAACC
0481 GACGCACCCT TGATCTGATT |
| in silico PCR Fragment |
0001 TGACCAACGC AACGGACATC GGTGCTAACG ACGTGGAGAT GGAGGTGGAT CCAACGGCGG
0061 AGACGCTGGC TGACGAGAAG AAGAACCAAG ATGTGGCCGC CGTGCAGGAG ATCCGCGAGC
0121 AGATTCGTCA GATTGAGAAG GGGGTAGCCT CGAAAGAGTC GCGCTTCATC CTGCGCGTCC
0181 TTCGCAATTT GCCCAACACT CGTCGCAAGC TGAACGGCGT CGTCTTCCGG AATCTTGCAC
0241 AGAGTATTTA CCCCGCTGGT GCAGATCGTG AGGCGGCCGT GGCTTTGATG CCCGCTGTGG
0301 AGAAAGACGC CACCGAGCTG CCCGATGTTC CCAAAAAACA AGTTGCCACC AAGGCTCCAA
0361 TCGCCGAGGT CGATGCCTAC TTCTACCTGC TCCTGCTGGT CAAGCTCATC GACGCCAGTG
0421 ATTTAAAGCG GGCCGGAATT AGCGCCGACG CCCTAATGGC CAAAATCTCC ATCCAAAACC
0481 GACGCACCCT TGATCTGATT |
| Assemble Data |
10484R-3.IR_full 1 TGACCAACGCAACGGACATCGGTGCTAACGACGTGGAGATGGAGGTGGATCCAACGGCGG 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10484R-3.in silico 1 TGACCAACGCAACGGACATCGGTGCTAACGACGTGGAGATGGAGGTGGATCCAACGGCGG 60
10484R-3.IR_full 61 AGACGCTGGCTGACGAGAAGAAGAACCAAGATGTGGCCGCCGTGCAGGAGATCCGCGAGC 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10484R-3.in silico 61 AGACGCTGGCTGACGAGAAGAAGAACCAAGATGTGGCCGCCGTGCAGGAGATCCGCGAGC 120
10484R-3.IR_full 121 AGATTCGTCAGATTGAGAAGGGGGTAGCCTCGAAAGAGTCGCGCTTCATCCTGCGCGTCC 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10484R-3.in silico 121 AGATTCGTCAGATTGAGAAGGGGGTAGCCTCGAAAGAGTCGCGCTTCATCCTGCGCGTCC 180
10484R-3.IR_full 181 TTCGCAATTTGCCCAACACTCGTCGCAAGCTGAACGGCGTCGTCTTCCGGAATCTTGCAC 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10484R-3.in silico 181 TTCGCAATTTGCCCAACACTCGTCGCAAGCTGAACGGCGTCGTCTTCCGGAATCTTGCAC 240
10484R-3.IR_full 241 AGAGTATTTACCCCGCTGGTGCAGATCGTGAGGCGGCCGTGGCTTTGATGCCCGCTGTGG 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10484R-3.in silico 241 AGAGTATTTACCCCGCTGGTGCAGATCGTGAGGCGGCCGTGGCTTTGATGCCCGCTGTGG 300
10484R-3.IR_full 301 AGAAAGACGCCACCGAGCTGCCCGATGTTCCCAAAAAACAAGTTGCCACCAAGGCTCCAA 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10484R-3.in silico 301 AGAAAGACGCCACCGAGCTGCCCGATGTTCCCAAAAAACAAGTTGCCACCAAGGCTCCAA 360
10484R-3.IR_full 361 TCGCCGAGGTCGATGCCTACTTCTACCTGCTCCTGCTGGTCAAGCTCATCGACGCCAGTG 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10484R-3.in silico 361 TCGCCGAGGTCGATGCCTACTTCTACCTGCTCCTGCTGGTCAAGCTCATCGACGCCAGTG 420
10484R-3.IR_full 421 ATTTAAAGCGGGCCGGAATTAGCGCCGACGCCCTAATGGCCAAAATCTCCATCCAAAACC 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10484R-3.in silico 421 ATTTAAAGCGGGCCGGAATTAGCGCCGACGCCCTAATGGCCAAAATCTCCATCCAAAACC 480
10484R-3.IR_full 481 GACGCACCCTTGATCTGATT 500
||||||||||||||||||||
10484R-3.in silico 481 GACGCACCCTTGATCTGATT 500
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_057952.3 |
CG10484-RA (Dox-A2), mRNA |
| 0 |
0 |
5 |
3 |
3 |
NM_139834.2 |
CG8615-RA (RpL18), mRNA |
| 0 |
0 |
3 |
2 |
3 |
NM_078727.2 |
CG4426-RA (ast), mRNA |
| 0 |
0 |
2 |
4 |
8 |
NM_169826.1 |
CG31224-RA (CG31224), mRNA |
| 0 |
0 |
2 |
3 |
4 |
NM_167224.1 |
CG32682-RA (CG32682), mRNA |
| 0 |
0 |
2 |
3 |
3 |
NM_140184.2 |
CG7628-RA (CG7628), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_134543.2 |
CG11738-RA (l(1)G0004), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_142481.1 |
CG7698-RA (CG7698), mRNA |
| 0 |
0 |
1 |
7 |
3 |
NM_132995.1 |
CG12991-RA, transcript variant A (CG12991), mRNA |
| 0 |
0 |
1 |
7 |
3 |
NM_167571.1 |
CG12991-RB, transcript variant B (CG12991), mRNA |
| 0 |
0 |
1 |
6 |
2 |
NM_164454.1 |
CG31671-RA (tho2), mRNA |
| 0 |
0 |
1 |
4 |
5 |
NM_206200.1 |
CG11206-RB, transcript variant B (CG11206), mRNA |
| 0 |
0 |
1 |
4 |
5 |
NM_137819.2 |
CG11206-RA, transcript variant A (CG11206), mRNA |
| 0 |
0 |
1 |
3 |
7 |
NM_140008.1 |
CG5741-RA (CG5741), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_142602.2 |
CG4424-RA (CG4424), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_139921.2 |
CG7972-RA (mus301), mRNA |
| 0 |
0 |
1 |
2 |
6 |
NM_080110.2 |
CG32356-RA, transcript variant A (ImpE1), mRNA |
| 0 |
0 |
1 |
2 |
3 |
NM_137512.2 |
CG5469-RD, transcript variant D (Gint3), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_143259.1 |
CG5521-RA (CG5521), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_137846.2 |
CG17807-RA (CG17807), mRNA |
| 0 |
0 |
0 |
6 |
4 |
NM_135156.2 |
CG13991-RA (CG13991), mRNA |
| 0 |
0 |
0 |
5 |
5 |
NM_139459.2 |
CG8993-RA (CG8993), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_144058.2 |
CG30158-RA (CG30158), mRNA |
| 0 |
0 |
0 |
4 |
11 |
NM_001042854.1 |
CG12473-RB, transcript variant B (stnB), mRNA |
| 0 |
0 |
0 |
4 |
11 |
NM_001042853.1 |
CG12473-RA, transcript variant A (stnB), mRNA |
| 0 |
0 |
0 |
4 |
11 |
NM_001042851.1 |
CG12500-RA, transcript variant A (stnA), mRNA |
| 0 |
0 |
0 |
4 |
11 |
NM_001042852.1 |
CG12500-RB, transcript variant B (stnA), mRNA |
| 0 |
0 |
0 |
4 |
6 |
NM_167101.1 |
CG3039-RB, transcript variant B (ogre), mRNA |
| 0 |
0 |
0 |
4 |
6 |
NM_080085.2 |
CG3039-RA, transcript variant A (ogre), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_132779.1 |
CG15028-RB, transcript variant B (CG15028), mRNA |
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