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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
10459R-1 |
| Symbol |
CG10459 |
Full Name |
CG10459 |
| CG No |
CG10459 |
Old CG No |
CG10459 |
| Synonyms |
CG10459 |
| Accession No (Link to NCBI) |
NM_136669.2 |
| Inserted Chr. |
ll |
| Insertional Mutation |
3 lethal |
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.
Zyxin links fat signaling to the hippo pathway.
PLoS Biol (2011) 9(6) e1000624
[
PubMed ID = 21666802
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
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| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgCGGCACGAGAACTAACTCTAT |
| Primer Seq. 3' |
aatctagaggtaccTGATGGATCCATTCTTCTCCC |
| Predicted Fragment Size |
437 |
| IR fragment full Seq |
0001 CGGCACGAGA ACTAACTCTA TTTTCCTGCC GCACCACGTC AAGTCGTTGG AGCTGCACCA
0061 TAGCGGCGAT ATAGTGACCA TAGCCTATGC CGGCGGAGTG ATCTTCCTGG ACCCCAAGAG
0121 CTTCGAAGTG CTCAAGCATC GCNAAGCTGC CCTACAAGGT GACTGCGGCA TCGCTGNAGT
0181 CCCAACAAGG GGATCTACGT GTGCGGCAAC AACATGGGCT ACTCCTTCAA ATACGACTAC
0241 GACACAGATG TTGATAGGGG TCTTTATGTA TCTCAGGAAC CATNCCGCAG TGCNTAGCAC
0301 TGAGCTTCAG CCCGGATGGC GAGGTGTGTG CCATTGGATC CCAAGATGGC AGTATCATTC
0361 TCTGGCAAAT GAACCCCAAA CAGGCGTCTG TAGTGGGCCA CCTCAAAGAC GAAGACGATG
0421 GGGAGAAGAA TGGATCCATC A |
| in silico PCR Fragment |
0001 CGGCACGAGA ACTAACTCTA TTTTCCTGCC GCACCACGTC AAGTCGTTGG AGCTGCACCA
0061 CAGCGGCGAT ATAGTGACCA TAGCCTATGC CGGCGGAGTG ATCTTCCTGG ACCCCAAGAG
0121 CTTCGAAGTG CTCAAGCATC GC-AAGCTGC CCTACAAGGT GACTGCGGCA TCGCTG-AGT
0181 CCCAACAAGG GAATCTACGT GTGCGGCAAC AACATGGGCT ACTCCTTCAA ATACGACTAC
0241 GACACAGATG TTGATAGGGG TCTTTATGTA TCTCAGGAAC CAT-CCGCAG TGC-TAGCAC
0301 TGAGCTTCAG CCCGGATGGC GAGGTGTGTG CCATTGGATC CCAAGATGGC AGTATCATTC
0361 TCTGGCAAAT GAACCCCAAA CAGGCGTCTG TAGTGGGCCA CCTCAAAGAC GAAGACGATG
0421 GGGAGAAGAA TGGATCCATC A |
| Assemble Data |
10459R-1.IR_full 1 CGGCACGAGAACTAACTCTATTTTCCTGCCGCACCACGTCAAGTCGTTGGAGCTGCACCA 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10459R-1.in silico 1 CGGCACGAGAACTAACTCTATTTTCCTGCCGCACCACGTCAAGTCGTTGGAGCTGCACCA 60
10459R-1.IR_full 61 TAGCGGCGATATAGTGACCATAGCCTATGCCGGCGGAGTGATCTTCCTGGACCCCAAGAG 120
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10459R-1.in silico 61 CAGCGGCGATATAGTGACCATAGCCTATGCCGGCGGAGTGATCTTCCTGGACCCCAAGAG 120
10459R-1.IR_full 121 CTTCGAAGTGCTCAAGCATCGCNAAGCTGCCCTACAAGGTGACTGCGGCATCGCTGNAGT 180
|||||||||||||||||||||| ||||||||||||||||||||||||||||||||| |||
10459R-1.in silico 121 CTTCGAAGTGCTCAAGCATCGC-AAGCTGCCCTACAAGGTGACTGCGGCATCGCTG-AGT 180
10459R-1.IR_full 181 CCCAACAAGGGGATCTACGTGTGCGGCAACAACATGGGCTACTCCTTCAAATACGACTAC 240
||||||||||| ||||||||||||||||||||||||||||||||||||||||||||||||
10459R-1.in silico 181 CCCAACAAGGGAATCTACGTGTGCGGCAACAACATGGGCTACTCCTTCAAATACGACTAC 240
10459R-1.IR_full 241 GACACAGATGTTGATAGGGGTCTTTATGTATCTCAGGAACCATNCCGCAGTGCNTAGCAC 300
||||||||||||||||||||||||||||||||||||||||||| ||||||||| ||||||
10459R-1.in silico 241 GACACAGATGTTGATAGGGGTCTTTATGTATCTCAGGAACCAT-CCGCAGTGC-TAGCAC 300
10459R-1.IR_full 301 TGAGCTTCAGCCCGGATGGCGAGGTGTGTGCCATTGGATCCCAAGATGGCAGTATCATTC 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10459R-1.in silico 301 TGAGCTTCAGCCCGGATGGCGAGGTGTGTGCCATTGGATCCCAAGATGGCAGTATCATTC 360
10459R-1.IR_full 361 TCTGGCAAATGAACCCCAAACAGGCGTCTGTAGTGGGCCACCTCAAAGACGAAGACGATG 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10459R-1.in silico 361 TCTGGCAAATGAACCCCAAACAGGCGTCTGTAGTGGGCCACCTCAAAGACGAAGACGATG 420
10459R-1.IR_full 421 GGGAGAAGAATGGATCCATCA 441
|||||||||||||||||||||
10459R-1.in silico 421 GGGAGAAGAATGGATCCATCA 441
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
419 |
0 |
0 |
4 |
NM_136669.2 |
CG10459-RA (CG10459), mRNA |
| 0 |
0 |
4 |
2 |
2 |
NM_165836.1 |
CG7776-RB, transcript variant B (E(Pc)), mRNA |
| 0 |
0 |
4 |
2 |
2 |
NM_078974.2 |
CG7776-RA, transcript variant A (E(Pc)), mRNA |
| 0 |
0 |
1 |
5 |
2 |
NM_141550.2 |
CG9716-RA (Cyp313b1), mRNA |
| 0 |
0 |
1 |
4 |
8 |
NM_079114.1 |
CG11290-RA (enok), mRNA |
| 0 |
0 |
1 |
4 |
7 |
NM_137273.2 |
CG8060-RA (CG8060), mRNA |
| 0 |
0 |
1 |
4 |
5 |
NM_132719.2 |
CG32604-RA, transcript variant A (l(1)G0007), mRNA |
| 0 |
0 |
1 |
2 |
7 |
NM_134768.3 |
CG17660-RA (CG17660), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_132235.2 |
CG32717-RB, transcript variant B (sdt), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_206653.1 |
CG32717-RE, transcript variant E (sdt), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_132236.3 |
CG32717-RA, transcript variant A (sdt), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_001038795.1 |
CG8683-RA (CG8683), mRNA |
| 0 |
0 |
0 |
7 |
5 |
NM_137960.2 |
CG3957-RA (CG3957), mRNA |
| 0 |
0 |
0 |
6 |
2 |
NM_135020.2 |
CG15626-RA, transcript variant A (CG15626), mRNA |
| 0 |
0 |
0 |
6 |
2 |
NM_164597.1 |
CG15626-RB, transcript variant B (CG15626), mRNA |
| 0 |
0 |
0 |
5 |
8 |
NM_080111.2 |
CG2684-RA (lds), mRNA |
| 0 |
0 |
0 |
5 |
5 |
NM_136335.2 |
CG1298-RA (CG1298), mRNA |
| 0 |
0 |
0 |
5 |
4 |
NM_138055.1 |
CG30419-RA (CG30419), mRNA |
| 0 |
0 |
0 |
5 |
3 |
NM_166021.1 |
CG6671-RC, transcript variant C (AGO1), mRNA |
| 0 |
0 |
0 |
5 |
3 |
NM_166020.1 |
CG6671-RA, transcript variant A (AGO1), mRNA |
| 0 |
0 |
0 |
5 |
3 |
NM_079010.2 |
CG6671-RB, transcript variant B (AGO1), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_079998.2 |
CG5912-RA (arr), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_079166.2 |
CG1044-RA, transcript variant A (dos), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_167956.1 |
CG1044-RB, transcript variant B (dos), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_142248.2 |
CG31183-RA (CG31183), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_143506.3 |
CG7921-RA, transcript variant A (Mgat2), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_001014684.1 |
CG7921-RB, transcript variant B (Mgat2), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_136972.2 |
CG12488-RA (CG12488), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_166200.2 |
CG8912-RA, transcript variant A (Psi), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_057775.3 |
CG8912-RB, transcript variant B (Psi), mRNA |
|
| Wing Disc |
GFP
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Puc
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Caspase
 |
DAPI
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Merge
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Figure
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JNK signal
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Caspase-3 signal
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