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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
10422R-1 |
| Symbol |
bam |
Full Name |
bag of marbles |
| CG No |
CG10422 |
Old CG No |
CG10422 |
| Synonyms |
BamC, BamF, Bam, ham, CG10422, alpha, fs(3)neo61, bam, BAM |
| Accession No (Link to NCBI) |
NM_057452.2 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Cho Y, Lai CM, Lin KY, Hsu HJ.
A Targeted RNAi Screen Reveals Drosophila Female-Sterile Genes That Control the Size of Germline Stem Cell Niche During Development.
G3 (Bethesda) (2018) 8(7) 2345-2354
[
PubMed ID = 29764959
]
[
RRC reference
]
Tokusumi T, Tokusumi Y, Hopkins DW, Shoue DA, Corona L, Schulz RA.
Germ line differentiation factor Bag of Marbles is a regulator of hematopoietic progenitor maintenance during Drosophila hematopoiesis.
Development (2011) 138(18) 3879-84
[
PubMed ID = 21813570
]
[
RRC reference
]
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| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgCAACCTGCACATCGGGCGTTT |
| Primer Seq. 3' |
aatctagaggtaccCACCTGCAGTTGGTAGCGCAC |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 CAACCTGCAC ATCGGGCGTT TTATCCGAAA TCCAGGAGAA CTTCGGTAGA CTCCGGTTGT
0061 GTGACGTTAC TGCACCACTC CTCGAATTCC ACGGTTTGGA TTGCTTGCAA CAGATTCAAA
0121 AGCGCTCGCG CCATTTTGCA TTCGACGGTT CTCCGGCCAA GAAGTCGCGA TCCGGAGGCG
0181 TGTTGGTCAC CGGGCCAAAG CAGAAGCAAC TGCAGAAGGA AAATGTGTGG AACCGGAAGA
0241 GTAAAGGCTC TGCGTCCGCG GATAATATTG AGAAACTGCC CATAACTATT GAGAAACTGC
0301 ATATGATTGG TCTGCACGGC GATTGCTTAG AGCACAACGC CGTGCTGCGT TTGATGAATC
0361 TGTTCAGATC CCTGCATGAT CACCTGACCG CCGATTTGGG CTTCTCGCGC CAAAACTCAA
0421 TGCCCTCGGA CTATCTGTTC GATATGCCGG TGAAGAGCAC GATGCCTAAG AGCTTGAATG
0481 TGCGCTACCA ACTGCAGGTG |
| in silico PCR Fragment |
0001 CAACCTGCAC ATCGGGCGTT TTATCCGAAA TCCAGGAGAA CTTCGGTAGA CTCCGGTTGT
0061 GTGACGTTAC TGCACCACTC CTCGAATTCC ACGGTTTGGA TTGCTTGCAA CAGATTCAAA
0121 AGCGCTCGCG CCATTTTGCA TTCGACGGTT CTCCGGCCAA GAAGTCGCGA TCCGGAGGCG
0181 TGTTGGTCAC CGGGCCAAAG CAGAAGCAAC TGCAGAAGGA AAATGTGTGG AACCGGAAGA
0241 GTAAAGGCTC TGCGTCCGCG GATAATATTG AGAAACTGCC CATAACTATT GAGAAACTGC
0301 ATATGATTGG TCTGCACGGC GATTGCTTAG AGCACAACGC CGTGCTGCGT TTGATGAATC
0361 TGTTCAGATC CCTGCATGAT CACCTGACCG CCGATTTGGG CTTCTCGCGC CAAAACTCAA
0421 TGCCCTCGGA CTATCTGTTC GATATGCCGG TGAAGAGCAC GATGCCTAAG AGCTTGAATG
0481 TGCGCTACCA ACTGCAGGTG |
| Assemble Data |
10422R-1.IR_full 1 CAACCTGCACATCGGGCGTTTTATCCGAAATCCAGGAGAACTTCGGTAGACTCCGGTTGT 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10422R-1.in silico 1 CAACCTGCACATCGGGCGTTTTATCCGAAATCCAGGAGAACTTCGGTAGACTCCGGTTGT 60
10422R-1.IR_full 61 GTGACGTTACTGCACCACTCCTCGAATTCCACGGTTTGGATTGCTTGCAACAGATTCAAA 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10422R-1.in silico 61 GTGACGTTACTGCACCACTCCTCGAATTCCACGGTTTGGATTGCTTGCAACAGATTCAAA 120
10422R-1.IR_full 121 AGCGCTCGCGCCATTTTGCATTCGACGGTTCTCCGGCCAAGAAGTCGCGATCCGGAGGCG 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10422R-1.in silico 121 AGCGCTCGCGCCATTTTGCATTCGACGGTTCTCCGGCCAAGAAGTCGCGATCCGGAGGCG 180
10422R-1.IR_full 181 TGTTGGTCACCGGGCCAAAGCAGAAGCAACTGCAGAAGGAAAATGTGTGGAACCGGAAGA 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10422R-1.in silico 181 TGTTGGTCACCGGGCCAAAGCAGAAGCAACTGCAGAAGGAAAATGTGTGGAACCGGAAGA 240
10422R-1.IR_full 241 GTAAAGGCTCTGCGTCCGCGGATAATATTGAGAAACTGCCCATAACTATTGAGAAACTGC 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10422R-1.in silico 241 GTAAAGGCTCTGCGTCCGCGGATAATATTGAGAAACTGCCCATAACTATTGAGAAACTGC 300
10422R-1.IR_full 301 ATATGATTGGTCTGCACGGCGATTGCTTAGAGCACAACGCCGTGCTGCGTTTGATGAATC 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10422R-1.in silico 301 ATATGATTGGTCTGCACGGCGATTGCTTAGAGCACAACGCCGTGCTGCGTTTGATGAATC 360
10422R-1.IR_full 361 TGTTCAGATCCCTGCATGATCACCTGACCGCCGATTTGGGCTTCTCGCGCCAAAACTCAA 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10422R-1.in silico 361 TGTTCAGATCCCTGCATGATCACCTGACCGCCGATTTGGGCTTCTCGCGCCAAAACTCAA 420
10422R-1.IR_full 421 TGCCCTCGGACTATCTGTTCGATATGCCGGTGAAGAGCACGATGCCTAAGAGCTTGAATG 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10422R-1.in silico 421 TGCCCTCGGACTATCTGTTCGATATGCCGGTGAAGAGCACGATGCCTAAGAGCTTGAATG 480
10422R-1.IR_full 481 TGCGCTACCAACTGCAGGTG 500
||||||||||||||||||||
10422R-1.in silico 481 TGCGCTACCAACTGCAGGTG 500
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
8 |
NM_057452.2 |
CG10422-RA (bam), mRNA |
| 0 |
0 |
3 |
3 |
2 |
NM_135908.2 |
CG18482-RA (CG18482), mRNA |
| 0 |
0 |
2 |
7 |
2 |
NM_135851.2 |
CG18146-RA, transcript variant A (CG18146), mRNA |
| 0 |
0 |
2 |
7 |
2 |
NM_176033.1 |
CG18146-RB, transcript variant B (CG18146), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_079337.2 |
CG9206-RA (Gl), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_133040.1 |
CG7192-RA, transcript variant A (CG7192), mRNA |
| 0 |
0 |
2 |
2 |
2 |
NM_167602.1 |
CG7192-RB, transcript variant B (CG7192), mRNA |
| 0 |
0 |
1 |
5 |
8 |
NM_134789.1 |
CG15356-RA (CG15356), mRNA |
| 0 |
0 |
1 |
5 |
2 |
NM_057439.2 |
CG2759-RA (w), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_057269.2 |
CG10236-RA (LanA), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_080260.2 |
CG5692-RA (raps), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_139845.2 |
CG8605-RA (CG8605), mRNA |
| 0 |
0 |
1 |
2 |
10 |
NM_133104.2 |
CG7282-RA (CG7282), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_136039.2 |
CG10211-RA (CG10211), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_079155.2 |
CG9102-RA (bab2), mRNA |
| 0 |
0 |
0 |
7 |
8 |
NM_079196.2 |
CG1232-RA, transcript variant A (tipE), mRNA |
| 0 |
0 |
0 |
7 |
8 |
NM_168067.1 |
CG1232-RB, transcript variant B (tipE), mRNA |
| 0 |
0 |
0 |
6 |
8 |
NM_169390.2 |
CG31374-RB, transcript variant B (CG31374), mRNA |
| 0 |
0 |
0 |
6 |
8 |
NM_176460.1 |
CG31374-RC, transcript variant C (CG31374), mRNA |
| 0 |
0 |
0 |
6 |
2 |
NM_135135.3 |
CG9098-RA, transcript variant A (CG9098), mRNA |
| 0 |
0 |
0 |
6 |
2 |
NM_164672.2 |
CG9098-RB, transcript variant B (CG9098), mRNA |
| 0 |
0 |
0 |
5 |
3 |
NM_001014583.1 |
CG32134-RB, transcript variant B (btl), mRNA |
| 0 |
0 |
0 |
4 |
10 |
NM_176539.1 |
CG33110-RA (CG33110), mRNA |
| 0 |
0 |
0 |
4 |
6 |
NM_138260.4 |
CG2199-RA, transcript variant A (CG2199), mRNA |
| 0 |
0 |
0 |
4 |
4 |
NM_136155.2 |
CG10628-RA (CG10628), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_136547.1 |
CG14748-RA (CG14748), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_079798.2 |
CG5507-RA (T48), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_140829.2 |
CG6812-RA (CG6812), mRNA |
| 0 |
0 |
0 |
3 |
14 |
NM_142521.1 |
CG6026-RA (CG6026), mRNA |
| 0 |
0 |
0 |
3 |
13 |
NM_057502.2 |
CG1322-RB, transcript variant B (zfh1), mRNA |
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