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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
1041R-1 |
| Symbol |
CG1041 |
Full Name |
CG1041 |
| CG No |
CG1041 |
Old CG No |
CG1041 |
| Synonyms |
CG1041 |
| Accession No (Link to NCBI) |
NM_141393.2 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
viable |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgAAGTTCCGTGGCAATGGAAAG |
| Primer Seq. 3' |
aatctagaggtaccGCGTGCACCATGTCGTTGAAT |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 AAGTTCCGTG GCAATGGAAA GCTTTTGTGG AATTTGACCA AGAACTCCTT GGCCCAGCAG
0061 TCTCCAAATG GGATCGCCAA GAAAGTGCTG CCCGCTAGCA GCTACAGCAC CGTCCAGAAG
0121 ACCATTCCCT TGGAGCAGCC CGAATCTGCT GAAGTACCAC GTCCTGCCGC TGGAGGAAAC
0181 GCTGAACCGC TTCATGACCA CTGTGGAACC TCTCCTGACG CCTGAGGAGT TTCAACGGCA
0241 AAAGGGAATC ACCTCCGAGT TTTTAAAAAA GCAGGGACGC GAACTGCAGC TGCTCCTGGA
0301 AGAAACCGGC AGCAAGGAGA AGAATTGGCT GGCCCACCGC TGGCTGAAGG CTGCCTATTT
0361 GACCTATCGA GATCCAGTCA CCGTGTTCGT GAGTCCTGGC ATGACCTTTC CCAAGCAAAA
0421 CTTCAGGGAC TCACGTGCTT TCGTGGACTA TACCGCCAGG GTTATCTATG GACTGGGAGA
0481 ATTCAACGAC ATGTGTCGCA CGC |
| in silico PCR Fragment |
0001 AAGTTCCGTG GCAATGGAAA GCTTTTGTGG AATTTGACCA AGAACTCCTT GGCCCAGCAG
0061 TCTCCAAATG GGATCGCCAA GAAAGTGCTG CCCGCTAGCA GCTACAGCAC CGTCCAGAAG
0121 ACCATTCCCT TGGAGCAG-C CGAATCTGCT GAAGTACCAC GTCCTGCCGC TGGAGGAAAC
0181 GCTGAACCGC TTCATGACCA CTGTGGAACC TCTCCTGACG CCTGAGGAGT TTCAACGGCA
0241 AAAGGGAATC ACCTCCGAGT TTTTAAAAAA GCAGGGACGC GAACTGCAGC TGCTCCTGGA
0301 AGAAACCGGC AGCAAGGAGA AGAATTGGCT GGCCCACCGC TGGCTGAAGG CTGCCTATTT
0361 GACCTATCGA GATCCAGTCA CCGTGTTCGT GAGTCCTGGC ATGACCTTTC CCAAGCAAAA
0421 CTTCAGGGAC TCACGTGCTT TCGTGGACTA TACCGCCAGG GTTATCTATG GACTGGGAGA
0481 ATTCAACGAC ATG-GT-GCA CGC |
| Assemble Data |
1041R-1.IR_full 1 AAGTTCCGTGGCAATGGAAAGCTTTTGTGGAATTTGACCAAGAACTCCTTGGCCCAGCAG 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1041R-1.in silico 1 AAGTTCCGTGGCAATGGAAAGCTTTTGTGGAATTTGACCAAGAACTCCTTGGCCCAGCAG 60
1041R-1.IR_full 61 TCTCCAAATGGGATCGCCAAGAAAGTGCTGCCCGCTAGCAGCTACAGCACCGTCCAGAAG 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1041R-1.in silico 61 TCTCCAAATGGGATCGCCAAGAAAGTGCTGCCCGCTAGCAGCTACAGCACCGTCCAGAAG 120
1041R-1.IR_full 121 ACCATTCCCTTGGAGCAGCCCGAATCTGCTGAAGTACCACGTCCTGCCGCTGGAGGAAAC 180
|||||||||||||||||| |||||||||||||||||||||||||||||||||||||||||
1041R-1.in silico 121 ACCATTCCCTTGGAGCAG-CCGAATCTGCTGAAGTACCACGTCCTGCCGCTGGAGGAAAC 180
1041R-1.IR_full 181 GCTGAACCGCTTCATGACCACTGTGGAACCTCTCCTGACGCCTGAGGAGTTTCAACGGCA 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1041R-1.in silico 181 GCTGAACCGCTTCATGACCACTGTGGAACCTCTCCTGACGCCTGAGGAGTTTCAACGGCA 240
1041R-1.IR_full 241 AAAGGGAATCACCTCCGAGTTTTTAAAAAAGCAGGGACGCGAACTGCAGCTGCTCCTGGA 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1041R-1.in silico 241 AAAGGGAATCACCTCCGAGTTTTTAAAAAAGCAGGGACGCGAACTGCAGCTGCTCCTGGA 300
1041R-1.IR_full 301 AGAAACCGGCAGCAAGGAGAAGAATTGGCTGGCCCACCGCTGGCTGAAGGCTGCCTATTT 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1041R-1.in silico 301 AGAAACCGGCAGCAAGGAGAAGAATTGGCTGGCCCACCGCTGGCTGAAGGCTGCCTATTT 360
1041R-1.IR_full 361 GACCTATCGAGATCCAGTCACCGTGTTCGTGAGTCCTGGCATGACCTTTCCCAAGCAAAA 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1041R-1.in silico 361 GACCTATCGAGATCCAGTCACCGTGTTCGTGAGTCCTGGCATGACCTTTCCCAAGCAAAA 420
1041R-1.IR_full 421 CTTCAGGGACTCACGTGCTTTCGTGGACTATACCGCCAGGGTTATCTATGGACTGGGAGA 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1041R-1.in silico 421 CTTCAGGGACTCACGTGCTTTCGTGGACTATACCGCCAGGGTTATCTATGGACTGGGAGA 480
1041R-1.IR_full 481 ATTCAACGACATGTGTCGCACGC 503
||||||||||||| || ||||||
1041R-1.in silico 481 ATTCAACGACATG-GT-GCACGC 503
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_141393.2 |
CG1041-RA, transcript variant A (CG1041), mRNA |
| 100 |
482 |
0 |
0 |
0 |
NM_001043219.1 |
CG1041-RB, transcript variant B (CG1041), mRNA |
| 0.62 |
3 |
4 |
2 |
3 |
NM_166443.1 |
CG9485-RA, transcript variant A (CG9485), mRNA |
| 0.62 |
3 |
4 |
2 |
3 |
NM_166444.1 |
CG9485-RB, transcript variant B (CG9485), mRNA |
| 0.62 |
3 |
4 |
2 |
3 |
NM_137733.2 |
CG9485-RC, transcript variant C (CG9485), mRNA |
| 0 |
0 |
13 |
19 |
28 |
NM_142348.1 |
CG5265-RA (CG5265), mRNA |
| 0 |
0 |
3 |
3 |
3 |
NM_132546.2 |
CG1492-RA (CG1492), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_170221.1 |
CG31087-RA (CG31087), mRNA |
| 0 |
0 |
1 |
5 |
4 |
NM_141324.1 |
CG1098-RA (Madm), mRNA |
| 0 |
0 |
1 |
4 |
7 |
NM_166689.1 |
CG3629-RB, transcript variant B (Dll), mRNA |
| 0 |
0 |
1 |
4 |
7 |
NM_079133.1 |
CG3629-RA, transcript variant A (Dll), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_144344.2 |
CG17178-RA (ACXE), mRNA |
| 0 |
0 |
1 |
4 |
2 |
NM_166516.1 |
CG11170-RB (CG11170), mRNA |
| 0 |
0 |
1 |
3 |
5 |
NM_137139.1 |
CG12858-RA (CG12858), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_078599.3 |
CG18657-RA (NetA), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_079571.1 |
CG8374-RA (dmt), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_139474.2 |
CG12182-RA (CG12182), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_139493.2 |
CG2083-RA (CG2083), mRNA |
| 0 |
0 |
1 |
2 |
5 |
NM_135898.2 |
CG15267-RA (CG15267), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_079985.2 |
CG5206-RA (bon), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_142641.1 |
CG5434-RA (Srp72), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_165888.2 |
CG30049-RA (CG30049), mRNA |
| 0 |
0 |
0 |
7 |
10 |
NM_168278.1 |
CG32352-RB, transcript variant B (CG32352), mRNA |
| 0 |
0 |
0 |
7 |
7 |
NM_170629.2 |
CG32352-RC, transcript variant C (CG32352), mRNA |
| 0 |
0 |
0 |
7 |
7 |
NM_168279.2 |
CG32352-RA, transcript variant A (CG32352), mRNA |
| 0 |
0 |
0 |
7 |
4 |
NM_137613.2 |
CG11099-RA (CG11099), mRNA |
| 0 |
0 |
0 |
7 |
3 |
NM_132464.2 |
CG1908-RA (CG1908), mRNA |
| 0 |
0 |
0 |
5 |
3 |
NM_134603.1 |
CG1489-RA (Pros45), mRNA |
| 0 |
0 |
0 |
5 |
2 |
NM_080096.2 |
CG7869-RA (SuUR), mRNA |
| 0 |
0 |
0 |
4 |
8 |
NM_168550.1 |
CG10089-RC, transcript variant C (CG10089), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
 |
JNK signal
| N |
 |
| |
|
| DB |
 |
| +- |
|
| DC |
 |
| + |
|
| DM |
 |
| +- |
|
| VM |
 |
| +- |
|
| VB |
 |
| |
|
|
Caspase-3 signal
| N |
|
| + |
|
| DB |
|
| + |
|
| DC |
|
| + |
|
| DM |
|
| + |
|
| VM |
|
| + |
|
| VB |
|
| + |
|
|
|
|
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