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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
discarded
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
10392R-1 |
| Symbol |
Ogt |
Full Name |
O-glycosyltransferase |
| CG No |
CG10392 |
Old CG No |
CG10392 |
| Synonyms |
CG10392, BcDNA:GH04245, Ogt |
| Accession No (Link to NCBI) |
|
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
Lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Yamamoto-Hino M, Yoshida H, Ichimiya T, Sakamura S, Maeda M, Kimura Y, Sasaki N, Aoki-Kinoshita KF, Kinoshita-Toyoda A, Toyoda H, Ueda R, Nishihara S, Goto S.
Phenotype-based clustering of glycosylation-related genes by RNAi-mediated gene silencing.
Genes Cells (2015) 20(6) 521-42
[
PubMed ID = 25940448
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgTGGACTGACTACGATATTCGC |
| Primer Seq. 3' |
aatctagaggtaccTTCTTGCACCCTTTGTGTAGC |
| Predicted Fragment Size |
505 |
| IR fragment full Seq |
|
| in silico PCR Fragment |
|
| Assemble Data |
10392R-1.IR full 1 TGGACTGACTACGATATTCGCATGAAAAAACTGGTTAGCATTGTCACTGAGCAGCTTGAA 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10392R-1.in silico 1 TGGACTGACTACGATATTCGCATGAAAAAACTGGTTAGCATTGTCACTGAGCAGCTTGAA 60
10392R-1.IR full 61 AAAAACCGTTTACCATCCGTGCATCCACACCACTCGATGCTTTATCCGTTGACACATGAT 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10392R-1.in silico 61 AAAAACCGTTTACCATCCGTGCATCCACACCACTCGATGCTTTATCCGTTGACACATGAT 120
10392R-1.IR full 121 TGTCGGAAAGCAATTGCAGC-CGACACGCTAATTTGTGTTTAGAAAAAGTTCACGTGCTT 180
|||||||||||||||||||| |||||||||||||||||||||||||||||||||||||||
10392R-1.in silico 121 TGTCGGAAAGCAATTGCAGCGCGACACGCTAATTTGTGTTTAGAAAAAGTTCACGTGCTT 180
10392R-1.IR full 181 CATAAAAAACCTTATAATTTCTTAAAGAAGTTGCCTACAAAAGGAAGGCTTCGAATAGGT 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10392R-1.in silico 181 CATAAAAAACCTTATAATTTCTTAAAGAAGTTGCCTACAAAAGGAAGGCTTCGAATAGGT 240
10392R-1.IR full 241 TATCTTAGCTCGGACTTCGGGAATCATCCTACCTCGCATTTAATGCAATCTGTGCCAGGT 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10392R-1.in silico 241 TATCTTAGCTCGGACTTCGGGAATCATCCTACCTCGCATTTAATGCAATCTGTGCCAGGT 300
10392R-1.IR full 301 CTTCATGATCGTTCAAAAGTGGAAATCTTTTGCTATGCCTTAAGTCCAGATGATGGTACA 360
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10392R-1.in silico 301 CTTCATGATCGTTCAAAAGTGGAAATCTTTTGCTATGCCTTAAGTCCAGATGATGGTACA 360
10392R-1.IR full 361 ACATTTCGACACAAAATCAGTCGAGAGTCTGAAAACTTTGTGGATCTTTCTCAGATCCCT 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10392R-1.in silico 361 ACATTTCGACACAAAATCAGTCGAGAGTCTGAAAACTTTGTGGATCTTTCTCAGATCCCT 420
10392R-1.IR full 421 TGTAATGGCAAGGCAGCGGACAAGATATTTAATGACGGTATACATATTTTGGTTAATATG 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10392R-1.in silico 421 TGTAATGGCAAGGCAGCGGACAAGATATTTAATGACGGTATACATATTTTGGTTAATATG 480
10392R-1.IR full 481 AATGGCTACACAAAGGGTGCAAGAA 505
|||||||||||||||||||||||||
10392R-1.in silico 481 AATGGCTACACAAAGGGTGCAAGAA 505
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
487 |
0 |
0 |
1 |
NM_078896.2 |
O-glycosyltransferase CG10392-RB, transcript variant B (Ogt), mRNA |
| 100 |
487 |
0 |
0 |
1 |
NM_165426.1 |
O-glycosyltransferase CG10392-RA, transcript variant A (Ogt), mRNA |
| 100 |
487 |
0 |
0 |
1 |
NM_165427.1 |
O-glycosyltransferase CG10392-RC, transcript variant C (Ogt), mRNA |
| 0 |
0 |
2 |
3 |
6 |
NM_139371.1 |
CG13917-RA (CG13917), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_001038777.1 |
CG33992-RA (CG33992), mRNA |
| 0 |
0 |
1 |
4 |
5 |
NM_001032256.1 |
Menl-1 CG7964-RB, transcript variant B (Menl-1), mRNA |
| 0 |
0 |
1 |
4 |
5 |
NM_001032255.1 |
Menl-1 CG7964-RC, transcript variant C (Menl-1), mRNA |
| 0 |
0 |
1 |
4 |
5 |
NM_001032257.1 |
Menl-1 CG7964-RA, transcript variant A (Menl-1), mRNA |
| 0 |
0 |
1 |
4 |
5 |
NM_001032253.1 |
CG7969-RB, transcript variant B (Menl-2), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_206095.1 |
CG13214-RB, transcript variant B (CG13214), mRNA |
| 0 |
0 |
1 |
3 |
3 |
NM_136816.1 |
CG13214-RA, transcript variant A (CG13214), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_135011.2 |
CG15631-RA (CG15631), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_142661.2 |
CG17273-RA (CG17273), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_140331.2 |
CG10627-RA (CG10627), mRNA |
| 0 |
0 |
0 |
4 |
6 |
NM_140176.1 |
CG6279-RA (CG6279), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_169022.1 |
Cdep CG31536-RA, transcript variant A (Cdep), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_001043211.1 |
Cdep CG31536-RE, transcript variant E (Cdep), mRNA |
| 0 |
0 |
0 |
4 |
5 |
NM_169023.1 |
Cdep CG31536-RB, transcript variant B (Cdep), mRNA |
| 0 |
0 |
0 |
4 |
3 |
NM_176213.1 |
muscleblind CG33197-RC, transcript variant C (mbl), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_135495.2 |
CG4778-RA (CG4778), mRNA |
| 0 |
0 |
0 |
4 |
2 |
NM_079985.2 |
bonus CG5206-RA (bon), mRNA |
| 0 |
0 |
0 |
3 |
6 |
NM_137093.4 |
CG30069-RA (CG30069), mRNA |
| 0 |
0 |
0 |
3 |
6 |
NM_080017.2 |
Smt3 activating enzyme 2 CG7528-RA (Uba2), mRNA |
| 0 |
0 |
0 |
3 |
4 |
NM_206353.1 |
dally-like CG32146-RB, transcript variant B (dlp), mRNA |
| 0 |
0 |
0 |
3 |
4 |
NM_079347.2 |
dally-like CG32146-RA, transcript variant A (dlp), mRNA |
| 0 |
0 |
0 |
3 |
4 |
NM_132750.2 |
CG9519-RA (CG9519), mRNA |
| 0 |
0 |
0 |
3 |
3 |
NM_134888.2 |
CG3165-RA (CG3165), mRNA |
| 0 |
0 |
0 |
3 |
3 |
NM_001015214.1 |
CG40160-PC.3 (CG40160), mRNA |
| 0 |
0 |
0 |
3 |
3 |
NM_169686.1 |
Synapse-associated protein 47kD CG8884-RD, transcript variant D (Sap47), mRNA |
| 0 |
0 |
0 |
3 |
3 |
NM_206184.1 |
bancal CG13425-RC, transcript variant C (bl), mRNA |
| 100 |
482 |
0 |
1 |
13 |
NM_078896.2 |
O-glycosyltransferase CG10392-RB, transcript variant B (Ogt), mRNA |
| 100 |
482 |
0 |
1 |
13 |
NM_165426.1 |
O-glycosyltransferase CG10392-RA, transcript variant A (Ogt), mRNA |
| 100 |
482 |
0 |
1 |
13 |
NM_165427.1 |
O-glycosyltransferase CG10392-RC, transcript variant C (Ogt), mRNA |
| 0 |
0 |
4 |
2 |
5 |
NM_130492.2 |
CG13366-RA, transcript variant A (CG13366), mRNA |
| 0 |
0 |
4 |
2 |
5 |
NM_001042789.1 |
CG13366-RB, transcript variant B (CG13366), mRNA |
| 0 |
0 |
3 |
5 |
10 |
NM_142597.2 |
Mediator complex subunit 25 CG12254-RA (MED25), mRNA |
| 0 |
0 |
2 |
3 |
11 |
NM_206681.2 |
discs large 1 CG1725-RH, transcript variant H (dlg1), mRNA |
| 0 |
0 |
2 |
3 |
11 |
NM_206683.2 |
discs large 1 CG1725-RB, transcript variant B (dlg1), mRNA |
| 0 |
0 |
2 |
3 |
8 |
NM_167282.2 |
discs large 1 CG1725-RA, transcript variant A (dlg1), mRNA |
| 0 |
0 |
2 |
3 |
8 |
NM_078565.3 |
discs large 1 CG1725-RD, transcript variant D (dlg1), mRNA |
| 0 |
0 |
2 |
3 |
8 |
NM_206682.2 |
discs large 1 CG1725-RG, transcript variant G (dlg1), mRNA |
| 0 |
0 |
2 |
3 |
8 |
NM_206684.2 |
discs large 1 CG1725-RE, transcript variant E (dlg1), mRNA |
| 0 |
0 |
2 |
2 |
11 |
NM_143018.2 |
CG5794-RD, transcript variant D (CG5794), mRNA |
| 0 |
0 |
2 |
2 |
11 |
NM_170158.1 |
CG5794-RB, transcript variant B (CG5794), mRNA |
| 0 |
0 |
1 |
8 |
15 |
NM_164981.1 |
CG18789-RA (CG18789), mRNA |
| 0 |
0 |
1 |
4 |
7 |
NM_167000.1 |
CG32778-RA (CG32778), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_166296.1 |
CG30332-RA (CG30332), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_176603.1 |
hephaestus CG31000-RH, transcript variant H (heph), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_164985.1 |
Translocase of outer membrane 70 CG6756-RB, transcript variant B (Tom70), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_135692.1 |
Translocase of outer membrane 70 CG6756-RA, transcript variant A (Tom70), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_164986.1 |
Translocase of outer membrane 70 CG6756-RC, transcript variant C (Tom70), mRNA |
| 0 |
0 |
0 |
12 |
38 |
NM_132525.1 |
CG15740-RA (CG15740), mRNA |
| 0 |
0 |
0 |
8 |
17 |
NM_144407.2 |
CG18787-RA (CG18787), mRNA |
| 0 |
0 |
0 |
7 |
11 |
NM_206148.1 |
CG30460-RD, transcript variant D (CG30460), mRNA |
| 0 |
0 |
0 |
7 |
10 |
NM_137342.2 |
CG30460-RC, transcript variant C (CG30460), mRNA |
| 0 |
0 |
0 |
7 |
10 |
NM_206150.1 |
CG30460-RB, transcript variant B (CG30460), mRNA |
| 0 |
0 |
0 |
7 |
10 |
NM_206149.1 |
CG30460-RA, transcript variant A (CG30460), mRNA |
| 0 |
0 |
0 |
6 |
39 |
NM_167343.1 |
CG32642-RC (CG32642), mRNA |
| 0 |
0 |
0 |
5 |
12 |
NM_136910.1 |
CG13165-RA (CG13165), mRNA |
| 0 |
0 |
0 |
5 |
7 |
NM_132794.2 |
CG18210-RA (CG18210), mRNA |
|
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