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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 10390R-2 
 Symbol mia  Full Name meiosis I arrest 
 CG No CG10390  Old CG No CG10390 
 Synonyms dTAF6L, dTAF[[II]]60, dmTAF6b, Taf6L, TAF, TFIID, Taf60-2, dTAF[[II]]60-2, TAF[[II]], TAF6L, CG10390, TAF60-2, ms(3)570, mia 
 Accession No (Link to NCBI) NM_141290.3 
 Inserted Chr. lll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgGTCTCAGAATGAAATGCCCGA 
 Primer Seq. 3' 
aatctagaggtaccGATAGCGCTTGCTGGGCTTTA 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 GTCTCAGAAT GAAATGCCCG ACAAGGACAA AAGTTTTCGC AGACATTCTT TGTCGCCGCG 
0061 TTCTCTATCC GCGATATTCT TCCACAGCAC TGGTCTGCAT CTGGACGACG GAGTAGCACA 
0121 GTGGCTATCC ATCAATGTTA AGGAAGACAT TACAAATCTG CTAAACGAGG CTGGAAAATA 
0181 NCATGCGGCG CATCCGGGAT CGGCGATTGC AACTGTCTCA CATCCAGCAT GCCGTCCGAA 
0241 TGCACGACGA CCTCTGCTAT GACATCTTTT TTAGGCTGGT CCATTGCGAC GATTGCAAAA 
0301 TGCCTCCTTC CCAAAAAGTA TTAAAAACCG TCCGCGAAGC TGNTAACCGC CGAAAAAAAG 
0361 GATGAACTTC TCGTTTCCTA CCCGGAATCC GTGCAGGAAT CTGTGCCGGA ACCCGTGCCG 
0421 GAGACTTCAT TGGAGCCGCC TCCCATGCAC ACCGGGTGGC TAAAGGTGGA GCAGGTGCTG 
0481 CTAAAGCCCA GCAAGCGCTA TC 
 in silico PCR Fragment 
0001 GTCTCAGAAT GAAATGCCCG ACAAGGACAA AAGTTTTCGC AGACATTCTT TGTCGCCGCG 
0061 TTCTCTATCC GCGATATTCT TCCACAGCAC TGGTCTGCAT CTGGACGACG GAGTAGCACA 
0121 GTGGCTATCC ATCAATGTTA AGGAAGACAT TACAAATCTG CTAAACGAGG CTGGAAAATA 
0181 -CATGCGGCG CATCCGGGAT CGGCGATTGC AACTGTCTCA CATCCAGCAT GCCGTCCGAA 
0241 TGCACGACGA CCTCTGCTAT GACATCTTTT TTAGGCTGGT CCATTGCGAC GATTGCAAAA 
0301 TGCCTCCTTC CCAAAAAGTA TTAAAAACCG TCCGCGAAGC TG-TAACCGC CGAAAAAAAG 
0361 GATGAACTTC TCGTTTCCTA CCCGGAATCC GTGCAGGAAT CTGTGCCGGA ACCCGTGCCG 
0421 GAGACTTCAT TGGAGCCGCC TCCCATGCAC ACCGGGTGGC TAAAGGTGGA GCAGGTGCTG 
0481 CTAAAGCCCA GCAAGCGCTA TC 
 Assemble Data 

10390R-2.IR_full       1   GTCTCAGAATGAAATGCCCGACAAGGACAAAAGTTTTCGCAGACATTCTTTGTCGCCGCG 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10390R-2.in silico     1   GTCTCAGAATGAAATGCCCGACAAGGACAAAAGTTTTCGCAGACATTCTTTGTCGCCGCG 60


10390R-2.IR_full       61  TTCTCTATCCGCGATATTCTTCCACAGCACTGGTCTGCATCTGGACGACGGAGTAGCACA 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10390R-2.in silico     61  TTCTCTATCCGCGATATTCTTCCACAGCACTGGTCTGCATCTGGACGACGGAGTAGCACA 120


10390R-2.IR_full       121 GTGGCTATCCATCAATGTTAAGGAAGACATTACAAATCTGCTAAACGAGGCTGGAAAATA 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10390R-2.in silico     121 GTGGCTATCCATCAATGTTAAGGAAGACATTACAAATCTGCTAAACGAGGCTGGAAAATA 180


10390R-2.IR_full        181 CATGCGGCGCATCCGGGATCGGCGATTGCAACTGTCTCACATCCAGCATGCCGTCCGAA 239
                            |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10390R-2.in silico      181 CATGCGGCGCATCCGGGATCGGCGATTGCAACTGTCTCACATCCAGCATGCCGTCCGAA 239


10390R-2.IR_full       241 TGCACGACGACCTCTGCTATGACATCTTTTTTAGGCTGGTCCATTGCGACGATTGCAAAA 299
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10390R-2.in silico     241 TGCACGACGACCTCTGCTATGACATCTTTTTTAGGCTGGTCCATTGCGACGATTGCAAAA 299


10390R-2.IR_full       301 TGCCTCCTTCCCAAAAAGTATTAAAAACCGTCCGCGAAGCTGNTAACCGCCGAAAAAAAG 359
                           |||||||||||||||||||||||||||||||||||||||||| |||||||||||||||||
10390R-2.in silico     301 TGCCTCCTTCCCAAAAAGTATTAAAAACCGTCCGCGAAGCTG-TAACCGCCGAAAAAAAG 359


10390R-2.IR_full       361 GATGAACTTCTCGTTTCCTACCCGGAATCCGTGCAGGAATCTGTGCCGGAACCCGTGCCG 419
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10390R-2.in silico     361 GATGAACTTCTCGTTTCCTACCCGGAATCCGTGCAGGAATCTGTGCCGGAACCCGTGCCG 419


10390R-2.IR_full       421 GAGACTTCATTGGAGCCGCCTCCCATGCACACCGGGTGGCTAAAGGTGGAGCAGGTGCTG 479
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10390R-2.in silico     421 GAGACTTCATTGGAGCCGCCTCCCATGCACACCGGGTGGCTAAAGGTGGAGCAGGTGCTG 479


10390R-2.IR_full       481 CTAAAGCCCAGCAAGCGCTATC 501
                           ||||||||||||||||||||||
10390R-2.in silico     481 CTAAAGCCCAGCAAGCGCTATC 501



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  4  NM_141290.3  CG10390-RA (mia), mRNA 
0   0  2  5  7  NM_134600.1  CG1494-RA (CG1494), mRNA 
0   0  2  3  2  NM_057425.2  CG9450-RA (tud), mRNA 
0   0  1  3  6  NM_167570.1  CG8465-RC, transcript variant C (l(1)G0222), mRNA 
0   0  1  3  6  NM_167569.1  CG8465-RB, transcript variant B (l(1)G0222), mRNA 
0   0  1  3  6  NM_132993.2  CG8465-RA, transcript variant A (l(1)G0222), mRNA 
0   0  1  3  3  NM_134486.1  CG14221-RA (CG14221), mRNA 
0   0  1  3  2  NM_169668.1  CG31461-RA (CG31461), mRNA 
0   0  1  2  7  NM_206628.1  CG32767-RB, transcript variant B (CG32767), mRNA 
0   0  1  2  7  NM_131975.3  CG32767-RA, transcript variant A (CG32767), mRNA 
0   0  1  2  7  NM_132852.1  CG8565-RA (CG8565), mRNA 
0   0  1  2  6  NM_137081.2  CG30483-RA (Prosap), mRNA 
0   0  1  2  6  NM_167277.1  CG11105-RA, transcript variant A (CG11105), mRNA 
0   0  1  2  6  NM_132471.1  CG11105-RB, transcript variant B (CG11105), mRNA 
0   0  1  2  5  NM_132004.2  CG4136-RA (CG4136), mRNA 
0   0  1  2  2  NM_080138.2  CG1618-RA (comt), mRNA 
0   0  0  7  2  NM_168822.2  CG8363-RB, transcript variant B (Paps), mRNA 
0   0  0  7  2  NM_168821.2  CG8363-RA, transcript variant A (Paps), mRNA 
0   0  0  7  2  NM_168823.2  CG8363-RC, transcript variant C (Paps), mRNA 
0   0  0  7  2  NM_079447.3  CG8363-RE, transcript variant E (Paps), mRNA 
0   0  0  7  2  NM_168824.2  CG8363-RD, transcript variant D (Paps), mRNA 
0   0  0  7  2  NM_001043152.1  CG8363-RF, transcript variant F (Paps), mRNA 
0   0  0  5  5  NM_142739.2  CG6678-RA (CG6678), mRNA 
0   0  0  5  3  NM_057882.3  CG1609-RA (Gcn2), mRNA 
0   0  0  5  3  NM_166293.1  CG17725-RB, transcript variant B (Pepck), mRNA 
0   0  0  5  3  NM_079060.2  CG17725-RA, transcript variant A (Pepck), mRNA 
0   0  0  5  2  NM_057526.3  CG5920-RA (sop), mRNA 
0   0  0  4  5  NM_142621.3  CG5077-RA, transcript variant A (CG5077), mRNA 
0   0  0  4  5  NM_169892.2  CG5077-RB, transcript variant B (CG5077), mRNA 
0   0  0  4  5  NM_169893.2  CG5077-RC, transcript variant C (CG5077), mRNA 

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