NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 10362R-1

Symbol CG10362 Full Name CG10362

CG No CG10362 Old CG No CG10362

Synonyms CG10362

Accession No (Link to NCBI) NM_132543.1

Inserted Chr. lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees lethal

Map Viewer

Reference
[Please submit your publication] Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Rauskolb C, Pan G, Reddy BV, Oh H, Irvine KD.
Zyxin links fat signaling to the hippo pathway.
PLoS Biol (2011) 9(6) e1000624 [ PubMed ID = 21666802 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgAACATTCTGCTGCTCTGCCTG

Primer Seq. 3'

aatctagaggtaccTCGATGCGCTGCTCATCCTTG

Predicted Fragment Size

IR fragment full Seq

0001 AACATTCTGC TGCTCTGCCT GATCTTTCTG TTGATCGGCG GAGTGGTGAT GATCCTGCTG 
0061 CAGTATCTCA TCTTCATCAA GTTCTCGAAT CTGCCCGACG AAAGCGAGGA GCAGAAGCAG 
0121 GCGAATGCCA AATACACCCT GCCATTGAAC ATCAAGCAGA CGGCCAGGAA CCTGAAGAGG 
0181 CTATCCAATT CCGGACTGGG TGGTGGCGCT GGGAATGGAT GCGAGAACCA TCAGGAGAGC 
0241 CTCCGACGGG CGCTCCGCCC TGGTCTCCCT CAACTTTGTC ATGCAGTTTC TCTTCCACGA 
0301 GTTCAAGAAC TCCAATCGGG TGCGGAAATG GTTCTATCGC AAGCTGTCCA TCGAGCTGGA 
0361 CGAACTGATT TCCAAGACTA CCACCGGAAA ACTGCTGGAC AAGCTAACGA TTAAAGAGCT 
0421 CGAGTTGGGC GACCAGTTTC CGGACGTCAA GTCGCTATCG GTGCACAACG TGGAATTGGA 
0481 CAAGGATGAG CAGCGCATCG A

in silico PCR Fragment

0001 AACATTCTGC TGCTCTGCCT GATCTTTCTG TTGATCGGCG GAGTGGTGAT GATCCTGCTG 
0061 CAGTATCTCA TCTTCATCAA GTTCTCGAAT CTGCCCGACG AAAGCGAGGA GCAGAAGCAG 
0121 GCGAATGCCA AATACACCCT GCCATTGAAC ATCAAGCAGA CGGCCAGGAA CCTGAAGAGG 
0181 CTATCCAATT CCGGACTGGG TGGTGGCGCT GGGAATGGAT GCGAGAACCA TCAGGAGAGC 
0241 CTCCGACGGG C-CTCCGCCC TGGTCTCCCT CAACTTTGTC ATGCAGTTTC TCTTCCACGA 
0301 GTTCAAGAAC TCCAATCGGG TGCGGAAATG GTTCTATCGC AAGCTGTCCA TCGAGCTGGA 
0361 CGAACTGATT TCCAAGACTA CCACCGGAAA ACTGCTGGAC AAGCTAACGA TTAAAGAGCT 
0421 CGAGTTGGGC GACCAGTTTC CGGACGTCAA GTCGCTATCG GTGCACAACG TGGAATTGGA 
0481 CAAGGATGAG CAGCGCATCG A

Assemble Data


10362R-1.IR_full       1   AACATTCTGCTGCTCTGCCTGATCTTTCTGTTGATCGGCGGAGTGGTGATGATCCTGCTG 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10362R-1.in silico     1   AACATTCTGCTGCTCTGCCTGATCTTTCTGTTGATCGGCGGAGTGGTGATGATCCTGCTG 60


10362R-1.IR_full       61  CAGTATCTCATCTTCATCAAGTTCTCGAATCTGCCCGACGAAAGCGAGGAGCAGAAGCAG 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10362R-1.in silico     61  CAGTATCTCATCTTCATCAAGTTCTCGAATCTGCCCGACGAAAGCGAGGAGCAGAAGCAG 120


10362R-1.IR_full       121 GCGAATGCCAAATACACCCTGCCATTGAACATCAAGCAGACGGCCAGGAACCTGAAGAGG 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10362R-1.in silico     121 GCGAATGCCAAATACACCCTGCCATTGAACATCAAGCAGACGGCCAGGAACCTGAAGAGG 180


10362R-1.IR_full       181 CTATCCAATTCCGGACTGGGTGGTGGCGCTGGGAATGGATGCGAGAACCATCAGGAGAGC 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10362R-1.in silico     181 CTATCCAATTCCGGACTGGGTGGTGGCGCTGGGAATGGATGCGAGAACCATCAGGAGAGC 240


10362R-1.IR_full       241 CTCCGACGGGCGCTCCGCCCTGGTCTCCCTCAACTTTGTCATGCAGTTTCTCTTCCACGA 300
                           ||||||||||| ||||||||||||||||||||||||||||||||||||||||||||||||
10362R-1.in silico     241 CTCCGACGGGC-CTCCGCCCTGGTCTCCCTCAACTTTGTCATGCAGTTTCTCTTCCACGA 300


10362R-1.IR_full       301 GTTCAAGAACTCCAATCGGGTGCGGAAATGGTTCTATCGCAAGCTGTCCATCGAGCTGGA 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10362R-1.in silico     301 GTTCAAGAACTCCAATCGGGTGCGGAAATGGTTCTATCGCAAGCTGTCCATCGAGCTGGA 360


10362R-1.IR_full       361 CGAACTGATTTCCAAGACTACCACCGGAAAACTGCTGGACAAGCTAACGATTAAAGAGCT 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10362R-1.in silico     361 CGAACTGATTTCCAAGACTACCACCGGAAAACTGCTGGACAAGCTAACGATTAAAGAGCT 420


10362R-1.IR_full       421 CGAGTTGGGCGACCAGTTTCCGGACGTCAAGTCGCTATCGGTGCACAACGTGGAATTGGA 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10362R-1.in silico     421 CGAGTTGGGCGACCAGTTTCCGGACGTCAAGTCGCTATCGGTGCACAACGTGGAATTGGA 480


10362R-1.IR_full       481 CAAGGATGAGCAGCGCATCGA 501
                           |||||||||||||||||||||
10362R-1.in silico     481 CAAGGATGAGCAGCGCATCGA 501

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_132543.1	CG10362-RA (CG10362), mRNA
0	0	3	2	3	NM_134526.2	CG12656-RA (CG12656), mRNA
0	0	2	3	7	NM_001038797.1	CG34059-RA (ppk16), mRNA
0	0	2	3	5	NM_140529.1	CG7650-RA (CG7650), mRNA
0	0	2	3	3	NM_142700.1	CG3337-RA (CG3337), mRNA
0	0	2	2	7	NM_057638.3	CG14472-RA (poe), mRNA
0	0	2	2	5	NM_175956.1	CG33003-RA (CG33003), mRNA
0	0	2	2	5	NM_168770.1	CG32199-RA (CG32199), mRNA
0	0	1	6	9	NM_166055.1	CG10108-RA (phyl), mRNA
0	0	1	6	4	NM_140557.3	CG6114-RA (CG6114), mRNA
0	0	1	4	3	NM_132891.1	CG9919-RA (CG9919), mRNA
0	0	1	4	2	NM_130646.2	CG14045-RA (CG14045), mRNA
0	0	1	3	10	NM_134718.4	CG31795-RA, transcript variant A (ia2), mRNA
0	0	1	3	5	NM_057892.3	CG4258-RA (dbe), mRNA
0	0	1	3	3	NM_136586.1	CG13748-RA (CG13748), mRNA
0	0	1	3	3	NM_079989.2	CG8053-RA, transcript variant A (eIF-1A), mRNA
0	0	1	3	3	NM_206509.1	CG8053-RB, transcript variant B (eIF-1A), mRNA
0	0	1	3	3	NM_133002.1	CG5800-RA (CG5800), mRNA
0	0	1	2	6	NM_138044.2	CG13569-RA, transcript variant A (CG13569), mRNA
0	0	1	2	6	NM_166663.1	CG13569-RB, transcript variant B (CG13569), mRNA
0	0	1	2	3	NM_144205.1	CG7796-RA (CG7796), mRNA
0	0	1	2	2	NM_078645.2	CG4252-RA (mei-41), mRNA
0	0	1	2	2	NM_144208.1	CG15210-RA (CG15210), mRNA
0	0	1	2	2	NM_141615.1	CG16734-RA (CG16734), mRNA
0	0	1	2	2	NM_143127.2	CG10560-RA (CG10560), mRNA
0	0	1	2	2	NM_136666.2	CG12926-RA (CG12926), mRNA
0	0	1	2	2	NM_136257.2	CG8677-RA (CG8677), mRNA
0	0	0	7	4	NM_142493.2	CG7717-RA, transcript variant A (Mekk1), mRNA
0	0	0	7	4	NM_169833.1	CG7717-RB, transcript variant B (Mekk1), mRNA
0	0	0	6	17	NM_132734.2	CG9411-RA (CG9411), mRNA

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