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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 10337R-2 
 Symbol CG10337  Full Name CG10337 
 CG No CG10337  Old CG No CG10337 
 Synonyms CG10337 
 Accession No (Link to NCBI) NM_136142.2 
 Inserted Chr. lll 
 Insertional Mutation  lethal 
 Phenotype induced by Act5C-GAL4 at 28 degrees lethal 
 Map Viewer
 Reference
[Please submit your publication] 		Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgCGAATTGCAAGGTTCCTATGG 
 Primer Seq. 3' 
aatctagaggtaccGAACGAGGAATCCGGACAATC 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 CGAATTGCAA GGTTCCTATG GAGGCCTCTA CTTTGGCTTC TGCACACTGA TCTCGCATGT 
0061 GCTTCTGGCG GGGATCACCG TAGCCATTGT TTACAAGTGT CTGGTGCTCA AATTGGTGCA 
0121 CACCGCCGGA CATGCCTTCT ACTGCACAAT CGGCTTTGTG TTTTTCATGG GCGAAGCATT 
0181 GCTGGTCCGG AATAGCGCAT ATTTGGAGTC CTCGTTGGGT CCATTGAACC TCAACCGCCT 
0241 GCACGCCGTC CTGGGGCTGT TGGCATTCCT GGTCGGAGCC GGCGGAATCG GAATCAAGAC 
0301 ATGGCAGAAG CTGGAGCGCA AACGGGAAGA CCCCAATGCC ACCGTTCGTC ACTTTAAGAG 
0361 CAACCATGCT TTTTACGGTA TAATCGGTTG TGCTCTTCTT CTGGGTAGCG TTCTGAGCGG 
0421 TCTTCCGCTG TATTTCATCA ACAGTGGCTT CGCCTTGAAA ATGCTTCATC GATTTTTCGG 
0481 ATTGTCCGGA TTCCTCGTTC  
 in silico PCR Fragment 
0001 CGAATTGCAA GGTTCCTATG GAGGCCTCTA CTTTGGCTTC TGCACACTGA TCTCGCATGT 
0061 GCTTCTGGCG GGGATCACCG TAGCCATTGT TTACAAGTGT CTGGTGCTCA AATTGGTGCA 
0121 CACCGCCGGA CATGCCTTCT ACTGCACAAT CGGCTTTGTG TTTTTCATGG GCGAAGCATT 
0181 GCTGGTCCGG AATAGCGCAT ATTTGGAGTC CTCGTTGGGT CCATTGAACC TCAACCGCCT 
0241 GCACGCCGTC CTGGGGCTGT TGGCATTCCT GGTCGGAGCC GGCGGAATCG GAATCAAGAC 
0301 ATGGCAGAAG CTGGAGCGCA AACGGGAAGA CCCCAATGCC ACCGTTCGTC ACTTTAAGAG 
0361 CAACCATGCT TTTTACGGTA TAATCGGTTG TGCTCTTCTT CTGGGTAGCG TTCTGAGCGG 
0421 TCTTCCGCTG TATTTCATCA ACAGTGGCTT CGCCTTGAAA ATGCTTCATC GATTTTTCGG 
0481 ATTGTCCGGA TTCCTCGTTC  
 Assemble Data 

10337R-2.IR_full       1   CGAATTGCAAGGTTCCTATGGAGGCCTCTACTTTGGCTTCTGCACACTGATCTCGCATGT 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10337R-2.in silico     1   CGAATTGCAAGGTTCCTATGGAGGCCTCTACTTTGGCTTCTGCACACTGATCTCGCATGT 60


10337R-2.IR_full       61  GCTTCTGGCGGGGATCACCGTAGCCATTGTTTACAAGTGTCTGGTGCTCAAATTGGTGCA 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10337R-2.in silico     61  GCTTCTGGCGGGGATCACCGTAGCCATTGTTTACAAGTGTCTGGTGCTCAAATTGGTGCA 120


10337R-2.IR_full       121 CACCGCCGGACATGCCTTCTACTGCACAATCGGCTTTGTGTTTTTCATGGGCGAAGCATT 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10337R-2.in silico     121 CACCGCCGGACATGCCTTCTACTGCACAATCGGCTTTGTGTTTTTCATGGGCGAAGCATT 180


10337R-2.IR_full       181 GCTGGTCCGGAATAGCGCATATTTGGAGTCCTCGTTGGGTCCATTGAACCTCAACCGCCT 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10337R-2.in silico     181 GCTGGTCCGGAATAGCGCATATTTGGAGTCCTCGTTGGGTCCATTGAACCTCAACCGCCT 240


10337R-2.IR_full       241 GCACGCCGTCCTGGGGCTGTTGGCATTCCTGGTCGGAGCCGGCGGAATCGGAATCAAGAC 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10337R-2.in silico     241 GCACGCCGTCCTGGGGCTGTTGGCATTCCTGGTCGGAGCCGGCGGAATCGGAATCAAGAC 300


10337R-2.IR_full       301 ATGGCAGAAGCTGGAGCGCAAACGGGAAGACCCCAATGCCACCGTTCGTCACTTTAAGAG 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10337R-2.in silico     301 ATGGCAGAAGCTGGAGCGCAAACGGGAAGACCCCAATGCCACCGTTCGTCACTTTAAGAG 360


10337R-2.IR_full       361 CAACCATGCTTTTTACGGTATAATCGGTTGTGCTCTTCTTCTGGGTAGCGTTCTGAGCGG 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10337R-2.in silico     361 CAACCATGCTTTTTACGGTATAATCGGTTGTGCTCTTCTTCTGGGTAGCGTTCTGAGCGG 420


10337R-2.IR_full       421 TCTTCCGCTGTATTTCATCAACAGTGGCTTCGCCTTGAAAATGCTTCATCGATTTTTCGG 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10337R-2.in silico     421 TCTTCCGCTGTATTTCATCAACAGTGGCTTCGCCTTGAAAATGCTTCATCGATTTTTCGG 480


10337R-2.IR_full       481 ATTGTCCGGATTCCTCGTTC 500
                           ||||||||||||||||||||
10337R-2.in silico     481 ATTGTCCGGATTCCTCGTTC 500



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_136142.2  CG10337-RA (CG10337), mRNA 
0   0  3  2  2  NM_141799.1  CG17187-RA (CG17187), mRNA 
0   0  2  2  2  NM_137990.2  CG4797-RA, transcript variant A (CG4797), mRNA 
0   0  2  2  2  NM_166625.1  CG4797-RB, transcript variant B (CG4797), mRNA 
0   0  1  3  2  NM_136595.1  CG13744-RA (CG13744), mRNA 
0   0  1  2  2  NM_132644.1  CG1622-RA (CG1622), mRNA 
0   0  0  6  2  NM_168744.1  CG32183-RB, transcript variant B (Ccn), mRNA 
0   0  0  6  2  NM_168745.1  CG32183-RA, transcript variant A (Ccn), mRNA 
0   0  0  5  5  NM_079436.1  CG32183-RA, transcript variant A (Ccn), mRNA, small, or homeotic discs 1 CG8887-RA (ash1), mRNA 
0   0  0  5  3  NM_206091.1  CG7734-RD, transcript variant D (shn), mRNA 
0   0  0  5  3  NM_057375.3  CG7734-RA, transcript variant A (shn), mRNA 
0   0  0  5  3  NM_134279.2  CG7734-RB, transcript variant B (shn), mRNA 
0   0  0  5  3  NM_057376.2  CG7734-RC, transcript variant C (shn), mRNA 
0   0  0  4  12  NM_167482.3  CG32580-RA (CG32580), mRNA 
0   0  0  4  8  NM_167637.1  CG32544-RA (CG32544), mRNA 
0   0  0  4  6  NM_134544.1  CG11734-RB (HERC2), mRNA 
0   0  0  4  3  NM_141771.1  CG14694-RA (CG14694), mRNA 
0   0  0  4  3  NM_164578.1  CG15427-RA, transcript variant A (tutl), mRNA 
0   0  0  4  2  NM_134742.2  CG5001-RA (CG5001), mRNA 
0   0  0  4  2  NM_142550.1  CG3517-RA (CG3517), mRNA 
0   0  0  3  7  NM_079785.2  CG8346-RA (HLHm3), mRNA 
0   0  0  3  6  NM_139462.1  CG13800-RA (CG13800), mRNA 
0   0  0  3  6  NM_001031867.1  CG33950-RF, transcript variant F (trol), mRNA 
0   0  0  3  5  NM_176360.1  CG32206-RC, transcript variant C (CG32206), mRNA 
0   0  0  3  5  NM_168794.2  CG32206-RB, transcript variant B (CG32206), mRNA 
0   0  0  3  5  NM_142974.2  CG17786-RA (CG17786), mRNA 
0   0  0  3  4  NM_142920.1  CG10230-RA, transcript variant A (Rpn9), mRNA 
0   0  0  3  4  NM_001038933.1  CG33999-RA (CG33999), mRNA 
0   0  0  3  4  NM_170088.1  CG10230-RB, transcript variant B (Rpn9), mRNA 
0   0  0  3  4  NM_132089.3  CG3847-RA (CG3847), mRNA 

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