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Notice:
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Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
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| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
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| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
10334R-2 |
| Symbol |
spi |
Full Name |
spitz |
| CG No |
CG10334 |
Old CG No |
CG10334 |
| Synonyms |
spi, CG10334, EP(2)2378, CT29014, Spitz, l(2)01068, Spi, l(2)s3547, l(2)37Fa, anon-WO0118547.158, s-spi |
| Accession No (Link to NCBI) |
NM_134291.3 |
| Inserted Chr. |
lll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
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Reference
[Please submit your publication]
|
Graca FA, Rai M, Hunt LC, Stephan A, Wang YD, Gordon B, Wang R, Quarato G, Xu B, Fan Y, Labelle M, Demontis F.
The myokine Fibcd1 is an endogenous determinant of myofiber size and mitigates cancer-induced myofiber atrophy.
Nat Commun (2022) 13(1) 2370
[
PubMed ID = 35501350
]
[
RRC reference
]
Wang C, Guo X, Xi R.
EGFR and Notch signaling respectively regulate proliferative activity and multiple cell lineage differentiation of Drosophila gastric stem cells.
Cell Res (2014) 24(5) 610-27
[
PubMed ID = 24603358
]
[
RRC reference
]
Xu N, Wang SQ, Tan D, Gao Y, Lin G, Xi R.
EGFR, Wingless and JAK/STAT signaling cooperatively maintain Drosophila intestinal stem cells.
Dev Biol (2011) 354(1) 31-43
[
PubMed ID = 21440535
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgCGCGCTCTTCGATCTCCTCGT |
| Primer Seq. 3' |
aatctagaggtaccGCCATCTGGACAGCACCGGTT |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 CGCGCTCTTC GATCTCCTCG TCCATGTCCG GCACgGCATT GCCGCCGACC CAGGCTCCAG
0061 TCACTAGTAG CACCACAATG CGCACCACCA CGACGACCAC GCCCAGGCCC AATATTACAT
0121 TCCCCACATA CAAATGTCCG GAAACCTTCG ATGCCTGGTA CTGTCTGAAC GATGCCCATT
0181 GCTTTGCGGT GAAGATAGCC GATCTACCGG TTTACAGCTG CGAGTGCGCG ATTGGCTTTA
0241 TGGGACAGCG ATGCGANATA CAAGGAGATC GACAATACTT ACCTGCCCAA GAGGCCGCGT
0301 CCGATGTTGG AGANAGGCGA GCATTGCCAG TGGAGCCATG TGTGCCCTGG TATTTATGCT
0361 GTTTGTCTGC CTGGCCTTCT ATTTGCGCTT CGAGCAGCGG GCTGCCAAGA AGGCCTACGA
0421 ACTGGAGCAG GAACTGCAGC AGGAATACGA CGATGACGAC GGCCAGTGCG AGTGCTGCCG
0481 CAACCGGTGC TGTCCAGATG GC |
| in silico PCR Fragment |
0001 CGCGCTCTTC GATCTCCTCG TCCATGTCCG GCACGGCATT GCCGCCGACC CAGGCTCCAG
0061 TCACTAGTAG CACCACAATG CGCACCACCA CGACGACCAC GCCCAGGCCC AATATTACAT
0121 TCCCCACATA CAAATGTCCG GAAACCTTCG ATGCCTGGTA CTGTTTGAAC GATGCCCATT
0181 GCTTTGCGGT GAAGATAGCC GATCTACCGG TTTACAGCTG CGAGTGCGCG ATTGGCTTTA
0241 TGGGACAGCG ATGCGA-ATA CAAGGAGATC GACAATACTT ACCTGCCCAA GAGGCCGCGT
0301 CCGATGTTGG AGA-AGGCGA GCATTGCCAG TGGAGCCATG TGTGCCCTGG TATTTATGCT
0361 GTTTGTCTGC CTGGCCTTCT ATTTGCGCTT CGAGCAGCGG GCTGCCAAGA AGGCCTACGA
0421 ACTGGAGCAG GAACTGCAGC AGGAATACGA CGATGACGAC GGCCAGTGCG AGTGCTGCCG
0481 CAACCGGTGC TGTCCAGATG GC |
| Assemble Data |
10334R-2.IR_full 1 CGCGCTCTTCGATCTCCTCGTCCATGTCCGGCACGGCATTGCCGCCGACCCAGGCTCCAG 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10334R-2.in silico 1 CGCGCTCTTCGATCTCCTCGTCCATGTCCGGCACGGCATTGCCGCCGACCCAGGCTCCAG 60
10334R-2.IR_full 61 TCACTAGTAGCACCACAATGCGCACCACCACGACGACCACGCCCAGGCCCAATATTACAT 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10334R-2.in silico 61 TCACTAGTAGCACCACAATGCGCACCACCACGACGACCACGCCCAGGCCCAATATTACAT 120
10334R-2.IR_full 121 TCCCCACATACAAATGTCCGGAAACCTTCGATGCCTGGTACTGTCTGAACGATGCCCATT 180
|||||||||||||||||||||||||||||||||||||||||||| |||||||||||||||
10334R-2.in silico 121 TCCCCACATACAAATGTCCGGAAACCTTCGATGCCTGGTACTGTTTGAACGATGCCCATT 180
10334R-2.IR_full 181 GCTTTGCGGTGAAGATAGCCGATCTACCGGTTTACAGCTGCGAGTGCGCGATTGGCTTTA 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10334R-2.in silico 181 GCTTTGCGGTGAAGATAGCCGATCTACCGGTTTACAGCTGCGAGTGCGCGATTGGCTTTA 240
10334R-2.IR_full 241 TGGGACAGCGATGCGANATACAAGGAGATCGACAATACTTACCTGCCCAAGAGGCCGCGT 300
|||||||||||||||| |||||||||||||||||||||||||||||||||||||||||||
10334R-2.in silico 241 TGGGACAGCGATGCGA-ATACAAGGAGATCGACAATACTTACCTGCCCAAGAGGCCGCGT 300
10334R-2.IR_full 301 CCGATGTTGGAGANAGGCGAGCATTGCCAGTGGAGCCATGTGTGCCCTGGTATTTATGCT 360
||||||||||||| ||||||||||||||||||||||||||||||||||||||||||||||
10334R-2.in silico 301 CCGATGTTGGAGA-AGGCGAGCATTGCCAGTGGAGCCATGTGTGCCCTGGTATTTATGCT 360
10334R-2.IR_full 361 GTTTGTCTGCCTGGCCTTCTATTTGCGCTTCGAGCAGCGGGCTGCCAAGAAGGCCTACGA 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10334R-2.in silico 361 GTTTGTCTGCCTGGCCTTCTATTTGCGCTTCGAGCAGCGGGCTGCCAAGAAGGCCTACGA 420
10334R-2.IR_full 421 ACTGGAGCAGGAACTGCAGCAGGAATACGACGATGACGACGGCCAGTGCGAGTGCTGCCG 480
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10334R-2.in silico 421 ACTGGAGCAGGAACTGCAGCAGGAATACGACGATGACGACGGCCAGTGCGAGTGCTGCCG 480
10334R-2.IR_full 481 CAACCGGTGCTGTCCAGATGGC 502
||||||||||||||||||||||
10334R-2.in silico 481 CAACCGGTGCTGTCCAGATGGC 502
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| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
0 |
NM_057561.4 |
CG10334-RF, transcript variant F (spi), mRNA |
| 100 |
482 |
0 |
0 |
0 |
NM_134291.3 |
CG10334-RA, transcript variant A (spi), mRNA |
| 100 |
482 |
0 |
0 |
0 |
NM_001032110.1 |
CG10334-RG, transcript variant G (spi), mRNA |
| 100 |
482 |
0 |
0 |
0 |
NM_134292.2 |
CG10334-RE, transcript variant E (spi), mRNA |
| 100 |
482 |
0 |
0 |
0 |
NM_134293.2 |
CG10334-RB, transcript variant B (spi), mRNA |
| 100 |
482 |
0 |
0 |
0 |
NM_134295.1 |
CG10334-RD, transcript variant D (spi), mRNA |
| 100 |
482 |
0 |
0 |
0 |
NM_134294.1 |
CG10334-RC, transcript variant C (spi), mRNA |
| 0.41 |
2 |
3 |
3 |
7 |
NM_130685.1 |
CG14420-RA (CG14420), mRNA |
| 0.2 |
1 |
5 |
3 |
3 |
NM_132668.1 |
CG15757-RA (CG15757), mRNA |
| 0.2 |
1 |
2 |
3 |
12 |
NM_078681.2 |
CG32540-RA (CCKLR-17D3), mRNA |
| 0 |
0 |
3 |
11 |
10 |
NM_078517.2 |
CG2175-RA, transcript variant A (dec-1), mRNA |
| 0 |
0 |
3 |
10 |
5 |
NM_167132.1 |
CG2175-RB, transcript variant B (dec-1), mRNA |
| 0 |
0 |
3 |
10 |
5 |
NM_167133.1 |
CG2175-RC, transcript variant C (dec-1), mRNA |
| 0 |
0 |
3 |
10 |
4 |
NM_176184.2 |
CG18250-RC, transcript variant C (Dg), mRNA |
| 0 |
0 |
3 |
6 |
6 |
NM_136882.2 |
CG30040-RA (jeb), mRNA |
| 0 |
0 |
3 |
5 |
25 |
NM_001043261.1 |
CG34157-RG, transcript variant G (Dys), mRNA |
| 0 |
0 |
3 |
5 |
25 |
NM_001043258.1 |
CG34157-RA, transcript variant A (Dys), mRNA |
| 0 |
0 |
3 |
5 |
25 |
NM_001043259.1 |
CG34157-RC, transcript variant C (Dys), mRNA |
| 0 |
0 |
3 |
5 |
25 |
NM_001043257.1 |
CG34157-RH, transcript variant H (Dys), mRNA |
| 0 |
0 |
3 |
5 |
25 |
NM_001043263.1 |
CG34157-RF, transcript variant F (Dys), mRNA |
| 0 |
0 |
3 |
5 |
23 |
NM_001043256.1 |
CG34157-RB, transcript variant B (Dys), mRNA |
| 0 |
0 |
3 |
4 |
23 |
NM_001043262.1 |
CG34157-RE, transcript variant E (Dys), mRNA |
| 0 |
0 |
3 |
4 |
22 |
NM_001043260.1 |
CG34157-RD, transcript variant D (Dys), mRNA |
| 0 |
0 |
3 |
3 |
3 |
NM_078520.2 |
CG2212-RA, transcript variant A (sws), mRNA |
| 0 |
0 |
3 |
3 |
3 |
NM_167141.1 |
CG2212-RB, transcript variant B (sws), mRNA |
| 0 |
0 |
3 |
2 |
2 |
NM_140372.1 |
CG14115-RA (CG14115), mRNA |
| 0 |
0 |
2 |
6 |
3 |
NM_170325.1 |
CG31064-RA, transcript variant A (CG31064), mRNA |
| 0 |
0 |
2 |
6 |
3 |
NM_143288.2 |
CG31064-RE, transcript variant E (CG31064), mRNA |
| 0 |
0 |
2 |
5 |
3 |
NM_164429.1 |
CG31666-RC, transcript variant C (CG31666), mRNA |
| 0 |
0 |
2 |
5 |
3 |
NM_164428.2 |
CG31666-RB, transcript variant B (CG31666), mRNA |
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