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NIG-RNAi Strains Detail
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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)
 Stock ID 10207R-3 
 Symbol NaPi-T  Full Name Na[+]-dependent inorganic phosphate cotransporter 
 CG No CG10207  Old CG No CG10207 
 Synonyms Dropo, Na/Pi-cotransporter, CG10207, NaPi-T 
 Accession No (Link to NCBI) NM_080377.2 
 Inserted Chr. ll 
 Insertional Mutation   
 Phenotype induced by Act5C-GAL4 at 28 degrees viable 
 Map Viewer
 Reference
[Please submit your publication] 		Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20 [ PubMed ID = 19838331 ] [ RRC reference ]  
 Comment  
 Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)
 Primer Seq. 5' 
aaggcctacatggccggaccgGCACTGTGCTGTGGTACATGA 
 Primer Seq. 3' 
aatctagaggtaccCGGAATCCATTTGGCAGTGAG 
 Predicted Fragment Size 
500 
 IR fragment full Seq 
0001 GCACGTGTGC TGTGGTACAT GACATTCATC GGTTTCATCG TGAACTACAT GATCCCGGAT 
0061 CAATCTGAAC ATTACCCATT GTGGACATGA TTGCCGGGAA GGGTGCTATT ACCTCAAATG 
0121 AAACCCACGA GAACTCTACG GACTTGGCTG CCCTTGCGGA AATGAATGAA CGATTTTCGC 
0181 TGGAGCGTTG GTTTTTGGAC TGGGCGAATA TTCCGTACGA AAAGAATGGA TTTCACTGGA 
0241 ACGAGAAGCA GCAGGGCGCT CTGTTGGGAT CATTTTTCTG GGCCCACTGG ACACTGCAGA 
0301 TTCCCGGCGG CATCTTGGCC ACCAAATATG GTACTAAACT GGTGTTTGGT TGGTCCAATG 
0361 GCATCGGTGT GTTCTGCTGT TTCCTTATAC CAATCGTATC GTACTGGAGC TACACGGGCT 
0421 TGATTATCCT GCGAGTATTC CAGGGGTGGA TAACGGGGCT TGGCCTGGCC ATCGAATGCA 
0481 CGTGCTCACT GCCAAATGGA TTCCG 
 in silico PCR Fragment 
0001 GCAC-TGTGC TGTGGTACAT GACATTCATC GGTTTCATCG TGAACTACAT GAT-CCGGAT 
0061 CAATCTGAAC ATTA-CCATT GTGGACATGA TTGCCGGGAA GGGTGCTATT ACCTCAAATG 
0121 AAACCCACGA GAACTCTACG GACTTGGCTG CCCTTGCGGA AATGAATGAA CGATTTTCGC 
0181 TGGAGCGTTG GTTTTTGGAC TGGGCGAATA TTCCGTACGA AAAGAATGGA TTTCACTGGA 
0241 ACGAGAAGCA GCAGGGCGCT CTGTTGGGAT CATTTTTCTG GGCCCACTGG ACACTGCAGA 
0301 TTCCCGGCGG CATCTTGGCC ACCAAATATG GTACTAAACT GGTGTTTGGT TGGTCCAATG 
0361 GCATCGGTGT GTTCTGCTGT TTCCTTATAC CAATCGTATC GTACTGGAGC TACACGGGCT 
0421 TGATTATCCT GCGAGTATTC CAGGGGTGGA TAAC-GGGCT TGGCCTGGCC ATCG-ATGCA 
0481 CGTGCTCACT GCCAAATGGA TTCCG 
 Assemble Data 

10207R-3.IR_full       1   GCACGTGTGCTGTGGTACATGACATTCATCGGTTTCATCGTGAACTACATGATCCCGGAT 60
                           |||| |||||||||||||||||||||||||||||||||||||||||||||||| ||||||
10207R-3.in silico     1   GCAC-TGTGCTGTGGTACATGACATTCATCGGTTTCATCGTGAACTACATGAT-CCGGAT 60


10207R-3.IR_full       61  CAATCTGAACATTACCCATTGTGGACATGATTGCCGGGAAGGGTGCTATTACCTCAAATG 120
                           |||||||||||||| |||||||||||||||||||||||||||||||||||||||||||||
10207R-3.in silico     61  CAATCTGAACATTA-CCATTGTGGACATGATTGCCGGGAAGGGTGCTATTACCTCAAATG 120


10207R-3.IR_full       121 AAACCCACGAGAACTCTACGGACTTGGCTGCCCTTGCGGAAATGAATGAACGATTTTCGC 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10207R-3.in silico     121 AAACCCACGAGAACTCTACGGACTTGGCTGCCCTTGCGGAAATGAATGAACGATTTTCGC 180


10207R-3.IR_full       181 TGGAGCGTTGGTTTTTGGACTGGGCGAATATTCCGTACGAAAAGAATGGATTTCACTGGA 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10207R-3.in silico     181 TGGAGCGTTGGTTTTTGGACTGGGCGAATATTCCGTACGAAAAGAATGGATTTCACTGGA 240


10207R-3.IR_full       241 ACGAGAAGCAGCAGGGCGCTCTGTTGGGATCATTTTTCTGGGCCCACTGGACACTGCAGA 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10207R-3.in silico     241 ACGAGAAGCAGCAGGGCGCTCTGTTGGGATCATTTTTCTGGGCCCACTGGACACTGCAGA 300


10207R-3.IR_full       301 TTCCCGGCGGCATCTTGGCCACCAAATATGGTACTAAACTGGTGTTTGGTTGGTCCAATG 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10207R-3.in silico     301 TTCCCGGCGGCATCTTGGCCACCAAATATGGTACTAAACTGGTGTTTGGTTGGTCCAATG 360


10207R-3.IR_full       361 GCATCGGTGTGTTCTGCTGTTTCCTTATACCAATCGTATCGTACTGGAGCTACACGGGCT 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10207R-3.in silico     361 GCATCGGTGTGTTCTGCTGTTTCCTTATACCAATCGTATCGTACTGGAGCTACACGGGCT 420


10207R-3.IR_full       421 TGATTATCCTGCGAGTATTCCAGGGGTGGATAACGGGGCTTGGCCTGGCCATCGAATGCA 480
                           |||||||||||||||||||||||||||||||||| ||||||||||||||||||| |||||
10207R-3.in silico     421 TGATTATCCTGCGAGTATTCCAGGGGTGGATAAC-GGGCTTGGCCTGGCCATCG-ATGCA 480


10207R-3.IR_full       481 CGTGCTCACTGCCAAATGGATTCCG 505
                           |||||||||||||||||||||||||
10207R-3.in silico     481 CGTGCTCACTGCCAAATGGATTCCG 505



 
 Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)
 Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch
cv 0mm 1mm 2mm 3mm target transcript off target name
100   482  0  0  0  NM_080377.2  CG10207-RA (NaPi-T), mRNA 
0   0  3  4  7  NM_079341.1  CG17285-RA, transcript variant A (Fbp1), mRNA 
0   0  3  4  7  NM_168578.1  CG17285-RB, transcript variant B (Fbp1), mRNA 
0   0  2  3  4  NM_141072.2  CG7177-RA (CG7177), mRNA 
0   0  1  3  6  NM_134837.2  CG9887-RA (VGlut), mRNA 
0   0  1  3  2  NM_169343.2  CG31147-RA (mthl11), mRNA 
0   0  1  2  3  NM_132453.1  CG2076-RA (CG2076), mRNA 
0   0  1  2  3  NM_142471.1  CG7691-RA (CG7691), mRNA 
0   0  1  2  2  NM_176585.1  CG7933-RB, transcript variant B (janA), mRNA 
0   0  1  2  2  NM_176586.2  CG7933-RA, transcript variant A (janA), mRNA 
0   0  1  2  2  NM_206588.1  CG7933-RC, transcript variant C (janA), mRNA 
0   0  1  2  2  NM_164873.1  CG18854-RB, transcript variant B (CG18854), mRNA 
0   0  1  2  2  NM_164872.1  CG18854-RA, transcript variant A (CG18854), mRNA 
0   0  1  2  2  NM_144442.2  CG18854-RC, transcript variant C (CG18854), mRNA 
0   0  1  2  2  NM_078823.2  CG16928-RA (mre11), mRNA 
0   0  1  2  2  NM_135469.2  CG16928-RA (mre11), mRNA,4,5-triphosphate kinase 1 CG4026-RA (IP3K1), mRNA 
0   0  0  5  3  NM_166917.1  CG11596-RB, transcript variant B (CG11596), mRNA 
0   0  0  5  3  NM_133114.2  CG32541-RA (CG32541), mRNA 
0   0  0  5  3  NM_130607.2  CG11596-RA, transcript variant A (CG11596), mRNA 
0   0  0  5  3  NM_166916.1  CG11596-RC, transcript variant C (CG11596), mRNA 
0   0  0  5  3  NM_164454.1  CG31671-RA (tho2), mRNA 
0   0  0  4  6  NM_132871.2  CG8939-RA (CG8939), mRNA 
0   0  0  4  5  NM_132320.2  CG12124-RA (CG12124), mRNA 
0   0  0  4  4  NM_168454.1  CG32083-RA (CG32083), mRNA 
0   0  0  4  3  NM_140663.2  CG9705-RA, transcript variant A (CG9705), mRNA 
0   0  0  4  2  NM_168801.1  CG9279-RB, transcript variant B (CG9279), mRNA 
0   0  0  4  2  NM_140867.1  CG9279-RA, transcript variant A (CG9279), mRNA 
0   0  0  4  2  NM_206420.1  CG33291-RA (CG33291), mRNA 
0   0  0  3  18  NM_001032051.1  CG33715-RD, transcript variant D (Msp-300), mRNA 
0   0  0  3  13  NM_132268.1  CG12772-RA (CG12772), mRNA 
 Wing Disc
GFP
 Puc
 Caspase
DAPI
 Merge
 Figure
JNK signal
N
 
DB
 
DC
+- 
DM
 
VM
 
VB
 
Caspase-3 signal
N
++ 
DB
+ 
DC
+ 
DM
 
VM
 
VB
 
Dcompartment   Penetrance
     

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