|
Notice:
|
|
Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.
|
|
| NIG-RNAi Strains Detail |
| Request : Click the "Order" button to request for this stock |
|
| Stock Detail (Last Update: November 2007_FlyBase version FB2007_11) |
| Stock ID |
10143R-4 |
| Symbol |
Adgf-E |
Full Name |
Adenosine deaminase-related growth factor E |
| CG No |
CG10143 |
Old CG No |
CG10143 |
| Synonyms |
ADGF-E, CG10143, adgfe, Adgf-E |
| Accession No (Link to NCBI) |
NM_137133.2 |
| Inserted Chr. |
ll |
| Insertional Mutation |
|
| Phenotype induced by Act5C-GAL4 at 28 degrees |
lethal |
| Map Viewer |
|
Reference
[Please submit your publication]
|
Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976
[
PubMed ID = 26488612
]
[
RRC reference
]
Umemori M, Habara O, Iwata T, Maeda K, Nishinoue K, Okabe A, Takemura M, Takahashi K, Saigo K, Ueda R, Adachi-Yamada T.
RNAi-mediated knockdown showing impaired cell survival in Drosophila wing imaginal disc.
Gene Regul Syst Bio (2009) 3 11-20
[
PubMed ID = 19838331
]
[
RRC reference
]
|
| Comment |
|
| Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24) |
| Primer Seq. 5' |
aaggcctacatggccggaccgATCCAGAAAAGATGACGCTGC |
| Primer Seq. 3' |
aatctagaggtaccTTCTCTGAGCTTTTCCATGGG |
| Predicted Fragment Size |
500 |
| IR fragment full Seq |
0001 ATCCAGAAAA GATGACGCTG CTCGAGATGC TGGGCACCAC CAATCTACGC AAGAGCACCC
0061 CCAAGGACTA CCGAATCCTG CGCGAGATCT TCAACTCCTT CGAACGATAC TCCGGAGTCG
0121 GCAACGACAT TCAATTGACC TTGCCAGAGC GCGCTGCCAA CGAGCTGATC ATGTGCGAAA
0181 AGAAGCGGGA GTACGAGAAG GGCATCATGG ATCCATCGAA GTTCGCGCCG GGACACCACA
0241 TCTTCCAGGT GCTCACCAAG GTGAAGAAGT CGCCGCTCTT CCAGATCCTG CGACGGATGC
0301 CCAANGGGTG CGGCCCTCAA GGCGCACGAC ACCTCCATGT GCAGTTCGAG GGTGGTGATC
0361 GAGATAACCT ATCGGGATAA CCTGTGGGTA TGTACCACAC AAAACGGCTG CCGTGTGGAG
0421 GAGTTCCGAT TCGCAAAGGA TAAGCCCAAG GACGTCANTT TCGAGAATGG CGAGTGGCAG
0481 CCCATGGAAA AGCTCAGAGA A |
| in silico PCR Fragment |
0001 ATCCAGAAAA GATGACGCTG CTCGAGATGC TGGGCACCAC CAATCTACGC AAGAGCACCC
0061 CCAAGGACTA CCGAATCCTG CGCGAGATCT TCAACTCCTT CGAACGATAC TCCGGAGTCG
0121 GCAACGACAT TCAATTGACC TTGCCAGAGC GCGCTGCCAA CGAGCTGATC ATGTGCGAAA
0181 AGAAGCGGGA GTACGAGAAG GGCATCATGG ATCCATCGAA GTTCGCGCCG GGACACCACA
0241 TCTTCCAGGT GCTCACCAAG GTGAAGAAGT CGCCGCTCTT CCAGATCCTG CGACGGATGC
0301 CCAA-GGGTG CGGCCCTCAA GGCGCACGAC ACCTCCATGT GCAGTTCGAG GGTGGTGATC
0361 GAGATAACCT ATCGGGATAA CCTGTGGGTA TGTACCACAC AAAACGGCTG CCGTGTGGAG
0421 GAGTTCCGAT TCGCAAAGGA TAAGCCCAAG GACGTCAGTT TCGAGAATGG CGAGTGGCAG
0481 CCCATGGAAA AGCTCAGAGA A |
| Assemble Data |
10143R-4.IR_full 1 ATCCAGAAAAGATGACGCTGCTCGAGATGCTGGGCACCACCAATCTACGCAAGAGCACCC 60
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10143R-4.in silico 1 ATCCAGAAAAGATGACGCTGCTCGAGATGCTGGGCACCACCAATCTACGCAAGAGCACCC 60
10143R-4.IR_full 61 CCAAGGACTACCGAATCCTGCGCGAGATCTTCAACTCCTTCGAACGATACTCCGGAGTCG 120
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10143R-4.in silico 61 CCAAGGACTACCGAATCCTGCGCGAGATCTTCAACTCCTTCGAACGATACTCCGGAGTCG 120
10143R-4.IR_full 121 GCAACGACATTCAATTGACCTTGCCAGAGCGCGCTGCCAACGAGCTGATCATGTGCGAAA 180
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10143R-4.in silico 121 GCAACGACATTCAATTGACCTTGCCAGAGCGCGCTGCCAACGAGCTGATCATGTGCGAAA 180
10143R-4.IR_full 181 AGAAGCGGGAGTACGAGAAGGGCATCATGGATCCATCGAAGTTCGCGCCGGGACACCACA 240
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10143R-4.in silico 181 AGAAGCGGGAGTACGAGAAGGGCATCATGGATCCATCGAAGTTCGCGCCGGGACACCACA 240
10143R-4.IR_full 241 TCTTCCAGGTGCTCACCAAGGTGAAGAAGTCGCCGCTCTTCCAGATCCTGCGACGGATGC 300
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10143R-4.in silico 241 TCTTCCAGGTGCTCACCAAGGTGAAGAAGTCGCCGCTCTTCCAGATCCTGCGACGGATGC 300
10143R-4.IR_full 301 CCAANGGGTGCGGCCCTCAAGGCGCACGACACCTCCATGTGCAGTTCGAGGGTGGTGATC 360
|||| |||||||||||||||||||||||||||||||||||||||||||||||||||||||
10143R-4.in silico 301 CCAA-GGGTGCGGCCCTCAAGGCGCACGACACCTCCATGTGCAGTTCGAGGGTGGTGATC 360
10143R-4.IR_full 361 GAGATAACCTATCGGGATAACCTGTGGGTATGTACCACACAAAACGGCTGCCGTGTGGAG 420
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10143R-4.in silico 361 GAGATAACCTATCGGGATAACCTGTGGGTATGTACCACACAAAACGGCTGCCGTGTGGAG 420
10143R-4.IR_full 421 GAGTTCCGATTCGCAAAGGATAAGCCCAAGGACGTCANTTTCGAGAATGGCGAGTGGCAG 480
||||||||||||||||||||||||||||||||||||| ||||||||||||||||||||||
10143R-4.in silico 421 GAGTTCCGATTCGCAAAGGATAAGCCCAAGGACGTCAGTTTCGAGAATGGCGAGTGGCAG 480
10143R-4.IR_full 481 CCCATGGAAAAGCTCAGAGAA 501
|||||||||||||||||||||
10143R-4.in silico 481 CCCATGGAAAAGCTCAGAGAA 501
|
| Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26) |
Off-target search result
cv : coefficient of variation
(0 mm hits / total siRNA count)
mm : mismatch
|
| cv |
0mm |
1mm |
2mm |
3mm |
target transcript |
off target name |
| 100 |
482 |
0 |
0 |
2 |
NM_137133.2 |
CG10143-RA (Adgf-E), mRNA |
| 0.2 |
1 |
2 |
2 |
1 |
NM_206353.1 |
CG32146-RB, transcript variant B (dlp), mRNA |
| 0.2 |
1 |
2 |
2 |
1 |
NM_079347.2 |
CG32146-RA, transcript variant A (dlp), mRNA |
| 0 |
0 |
5 |
4 |
3 |
NM_057825.3 |
CG2928-RA (Reg-5), mRNA |
| 0 |
0 |
4 |
3 |
4 |
NM_167346.1 |
CG4353-RA, transcript variant A (hep), mRNA |
| 0 |
0 |
4 |
3 |
4 |
NM_078587.2 |
CG4353-RC, transcript variant C (hep), mRNA |
| 0 |
0 |
3 |
5 |
5 |
NM_057973.3 |
CG2864-RA (Parg), mRNA |
| 0 |
0 |
3 |
2 |
9 |
NM_167266.1 |
CG12101-RB, transcript variant B (Hsp60), mRNA |
| 0 |
0 |
3 |
2 |
9 |
NM_078560.2 |
CG12101-RA, transcript variant A (Hsp60), mRNA |
| 0 |
0 |
3 |
2 |
6 |
NM_205900.1 |
CG3327-RC, transcript variant C (E23), mRNA |
| 0 |
0 |
3 |
2 |
6 |
NM_079909.2 |
CG3327-RA, transcript variant A (E23), mRNA |
| 0 |
0 |
3 |
2 |
5 |
NM_134924.1 |
CG3327-RB, transcript variant B (E23), mRNA |
| 0 |
0 |
2 |
11 |
6 |
NM_079925.2 |
CG13570-RA (spag), mRNA |
| 0 |
0 |
2 |
6 |
6 |
NM_132967.1 |
CG5004-RA (CG5004), mRNA |
| 0 |
0 |
2 |
6 |
3 |
NM_134848.1 |
CG3214-RA (CG3214), mRNA |
| 0 |
0 |
2 |
3 |
2 |
NM_080292.2 |
CG3972-RA (Cyp4g1), mRNA |
| 0 |
0 |
2 |
2 |
4 |
NM_078509.2 |
CG3929-RA (dx), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_080259.1 |
CG14066-RA, transcript variant A (larp), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_170366.1 |
CG14066-RB, transcript variant B (larp), mRNA |
| 0 |
0 |
2 |
2 |
3 |
NM_170365.1 |
CG14066-RC, transcript variant C (larp), mRNA |
| 0 |
0 |
1 |
6 |
5 |
NM_135306.2 |
CG7144-RA (CG7144), mRNA |
| 0 |
0 |
1 |
4 |
3 |
NM_132900.2 |
CG9947-RA (CG9947), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_133031.1 |
CG6847-RA (CG6847), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_136412.2 |
CG4445-RA (pgant3), mRNA |
| 0 |
0 |
1 |
3 |
4 |
NM_080044.2 |
CG13906-RA (nerfin-1), mRNA |
| 0 |
0 |
1 |
3 |
2 |
NM_140688.2 |
CG7728-RA (CG7728), mRNA |
| 0 |
0 |
1 |
2 |
4 |
NM_143134.1 |
CG11909-RA (CG11909), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_170240.2 |
CG31094-RA, transcript variant A (LpR1), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_206573.1 |
CG31094-RB, transcript variant B (LpR1), mRNA |
| 0 |
0 |
1 |
2 |
2 |
NM_176565.1 |
CG31092-RB, transcript variant B (LpR2), mRNA |
|
| Wing Disc |
GFP
 |
Puc
 |
Caspase
 |
DAPI
 |
Merge
 |
Figure
 |
|
JNK signal
|
Caspase-3 signal
| N |
 |
| + |
|
| DB |
 |
| + |
|
| DC |
 |
| ++ |
|
| DM |
 |
| + |
|
| VM |
 |
| |
|
| VB |
 |
| |
|
|
|
|
|
|
