NIG-Fly - Fly Stocks of National Institute of Genetics -

Notice:
Suspension of shipments during Golden Week Holidays: From 30 April to 14 May, deadline 17 April.

NIG-RNAi Strains Detail

Request : Click the "Order" button to request for this stock

discarded

Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 10142R-3

Symbol Ance-5 Full Name Ance-5

CG No CG10142 Old CG No CG10142

Synonyms CG10142, Ance-5

Accession No (Link to NCBI) NM_133164.2

Inserted Chr. lll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees lethal

Map Viewer

Reference
[Please submit your publication] Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgTTCTGCTCTCCGTGACCTTCG

Primer Seq. 3'

aatctagaggtaccCCAGGCCTTCCAGTTGATGAC

Predicted Fragment Size

IR fragment full Seq

0001 TTCTGCTCTC CGTGACCTTC GGAATCGGCC AGTCCAGCTA TGAACTGGCT ACCAAGCAGA 
0061 TACTTGATAG AGCCAAAGAC CGCATGCGCC ACGTGTGGAG TCTGAACCGC AGGATCTTTG 
0121 TGCAGCTCAC GGCCAAAGGA AAAGTCACCT CTGGGCGGAC AGGTTCTCAA CTCCAAGCTG 
0181 GAGGTAGAGG CGGAGACNTA TCGACTATTT TACGACTTAG CGGGCAATCT CAGCGTTGTG 
0241 TCAGTGGCTG AATTGAATGA CCCACTTCTT CNTAGGGCGC GTGCAGCGAA TGGCCAAGTT 
0301 GCAGCTGCAG GGACTGCGGC CAAAGGACTA CGAGCAGGCC AAGGACCTTC TACGTCAGAT 
0361 CCATAACTTT GTCAACGGAC CCCTGGTCTG CCCTTACGAG GANCCTGCNC CGcccgtGGT 
0421 TCGCTGGCCA TGTACCCGCA GATCATGAAC AAAAACATGC ACACCAAGCT ATACGAGGAT 
0481 CTGGTCATCA ACTGGAAGGC CTGG

in silico PCR Fragment

0001 TTCTGCTCTC CGTGACCTTC GGAATCGGCC AGTCCAGCTA TGAACTGGCT ACCAAGCAGA 
0061 TACTTGATAG AGCCAAAGAC CGCATGCGCC ACGTGTGGAG TCTGAACCGC AGGATCTTTG 
0121 TGCAGCTCAC GGCCAAAGG- AAAGTCACCT CTGGGCGGAC AGGTTCTCAA CTCCAAGCTG 
0181 GAGGTAGAGG CGGAGACCTA TCGACTATTT TACGACTTAG CGGGCAATCT CAGCGTTGTG 
0241 TCAGTGGCTG AATTGAATGA CCCACTTCTT CGTA-GGCGC GTGCAGCGAA TGGCCAAGTT 
0301 GCAGCTGCAG GGACTGCGGC CAAAGGACTA CGAGCAGGCC AAGGACCTTC TACGTCAGAT 
0361 CCATAACTTT GTCAACGGAC CCCTGGTCTG CCCTTACGAG GA--CTGCTC CGCCCGTGGT 
0421 TCGCTGGCCA TGTACCCGCA GATCATGAAC AAAAACATGC ACACCAAGCT ATACGAGGAT 
0481 CTGGTCATCA ACTGGAAGGC CTGG

Assemble Data


10142R-3.IR_full       1   TTCTGCTCTCCGTGACCTTCGGAATCGGCCAGTCCAGCTATGAACTGGCTACCAAGCAGA 60
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10142R-3.in silico     1   TTCTGCTCTCCGTGACCTTCGGAATCGGCCAGTCCAGCTATGAACTGGCTACCAAGCAGA 60


10142R-3.IR_full       61  TACTTGATAGAGCCAAAGACCGCATGCGCCACGTGTGGAGTCTGAACCGCAGGATCTTTG 120
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10142R-3.in silico     61  TACTTGATAGAGCCAAAGACCGCATGCGCCACGTGTGGAGTCTGAACCGCAGGATCTTTG 120


10142R-3.IR_full       121 TGCAGCTCACGGCCAAAGGAAAAGTCACCTCTGGGCGGACAGGTTCTCAACTCCAAGCTG 180
                           ||||||||||||||||||| ||||||||||||||||||||||||||||||||||||||||
10142R-3.in silico     121 TGCAGCTCACGGCCAAAGG-AAAGTCACCTCTGGGCGGACAGGTTCTCAACTCCAAGCTG 180


10142R-3.IR_full       181 GAGGTAGAGGCGGAGACNTATCGACTATTTTACGACTTAGCGGGCAATCTCAGCGTTGTG 240
                           ||||||||||||||||| ||||||||||||||||||||||||||||||||||||||||||
10142R-3.in silico     181 GAGGTAGAGGCGGAGACCTATCGACTATTTTACGACTTAGCGGGCAATCTCAGCGTTGTG 240


10142R-3.IR_full       241 TCAGTGGCTGAATTGAATGACCCACTTCTTCNTAGGGCGCGTGCAGCGAATGGCCAAGTT 300
                           ||||||||||||||||||||||||||||||| || |||||||||||||||||||||||||
10142R-3.in silico     241 TCAGTGGCTGAATTGAATGACCCACTTCTTCGTA-GGCGCGTGCAGCGAATGGCCAAGTT 300


10142R-3.IR_full       301 GCAGCTGCAGGGACTGCGGCCAAAGGACTACGAGCAGGCCAAGGACCTTCTACGTCAGAT 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10142R-3.in silico     301 GCAGCTGCAGGGACTGCGGCCAAAGGACTACGAGCAGGCCAAGGACCTTCTACGTCAGAT 360


10142R-3.IR_full       361 CCATAACTTTGTCAACGGACCCCTGGTCTGCCCTTACGAGGANCCTGCNCCGCCCGTGGT 420
                           ||||||||||||||||||||||||||||||||||||||||||  |||| |||||||||||
10142R-3.in silico     361 CCATAACTTTGTCAACGGACCCCTGGTCTGCCCTTACGAGGA--CTGCTCCGCCCGTGGT 420


10142R-3.IR_full       421 TCGCTGGCCATGTACCCGCAGATCATGAACAAAAACATGCACACCAAGCTATACGAGGAT 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10142R-3.in silico     421 TCGCTGGCCATGTACCCGCAGATCATGAACAAAAACATGCACACCAAGCTATACGAGGAT 480


10142R-3.IR_full       481 CTGGTCATCAACTGGAAGGCCTGG 504
                           ||||||||||||||||||||||||
10142R-3.in silico     481 CTGGTCATCAACTGGAAGGCCTGG 504

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_133164.2	CG10142-RA (Ance-5), mRNA
0	0	3	4	2	NM_206229.1	CG9097-RA, transcript variant A (bab1), mRNA
0	0	3	2	2	NM_135891.3	CG4140-RA (CG4140), mRNA
0	0	2	3	2	NM_057258.2	CG10699-RA, transcript variant A (Lim3), mRNA
0	0	2	3	2	NM_165277.1	CG10699-RB, transcript variant B (Lim3), mRNA
0	0	2	2	4	NM_141572.1	CG11768-RA (CG11768), mRNA
0	0	1	4	12	NM_132792.2	CG6227-RA (CG6227), mRNA
0	0	1	3	3	NM_141942.2	CG5844-RA (CG5844), mRNA
0	0	1	3	2	NM_140021.1	CG5280-RA (CG5280), mRNA
0	0	1	3	2	NM_143493.2	CG31040-RA (Cog7), mRNA
0	0	1	2	5	NM_167073.1	CG3314-RA, transcript variant A (RpL7A), mRNA
0	0	1	2	5	NM_080362.2	CG31868-RA (Samuel), mRNA
0	0	1	2	5	NM_001014466.1	CG15624-RB, transcript variant B (hoe2), mRNA
0	0	1	2	5	NM_135034.1	CG15624-RA, transcript variant A (hoe2), mRNA
0	0	1	2	5	NM_078508.4	CG3314-RD, transcript variant D (RpL7A), mRNA
0	0	1	2	5	NM_141858.1	CG17360-RA (CG17360), mRNA
0	0	1	2	5	NM_167075.1	CG3314-RC, transcript variant C (RpL7A), mRNA
0	0	1	2	5	NM_130545.2	CG11411-RA (fs(1)N), mRNA
0	0	1	2	4	NM_168396.1	CG6640-RB, transcript variant B (CG6640), mRNA
0	0	1	2	4	NM_140125.1	CG6640-RA, transcript variant A (CG6640), mRNA
0	0	1	2	4	NM_079648.2	CG5083-RA (Rbf2), mRNA
0	0	1	2	4	NM_142268.1	CG6006-RA (CG6006), mRNA
0	0	1	2	3	NM_136599.2	CG8193-RA (CG8193), mRNA
0	0	1	2	2	NM_079944.2	CG3715-RA (Shc), mRNA
0	0	1	2	2	NM_132577.1	CG2556-RA (CG2556), mRNA
0	0	0	5	3	NM_079260.1	CG4978-RA (Mcm7), mRNA
0	0	0	4	9	NM_080536.2	CG4013-RA, transcript variant A (Smr), mRNA
0	0	0	4	9	NM_167334.1	CG4013-RB, transcript variant B (Smr), mRNA
0	0	0	4	9	NM_167335.1	CG4013-RC, transcript variant C (Smr), mRNA
0	0	0	4	8	NM_142521.1	CG6026-RA (CG6026), mRNA

Back