NIG-Fly - Fly Stocks of National Institute of Genetics -

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NIG-RNAi Strains Detail

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Stock Detail (Last Update: November 2007_FlyBase version FB2007_11)

Stock ID 10086R-7

Symbol CG10086 Full Name CG10086

CG No CG10086 Old CG No CG10086

Synonyms CG10086

Accession No (Link to NCBI) NM_141479.2

Inserted Chr. ll

Insertional Mutation

Phenotype induced by Act5C-GAL4 at 28 degrees lethal

Map Viewer

Reference
[Please submit your publication] Tiwari P, Kumar A, Das RN, Malhotra V, VijayRaghavan K.
A Tendon Cell Specific RNAi Screen Reveals Novel Candidates Essential for Muscle Tendon Interaction.
PLoS One (2015) 10(10) e0140976 [ PubMed ID = 26488612 ] [ RRC reference ]

Comment

Sequence (Last Update: July 10, 2007_NCBI RefSeq Release 24)

Primer Seq. 5'

aaggcctacatggccggaccgGGCAAGGTGATTGTCAATCTG

Primer Seq. 3'

aatctagaggtaccTGCTGGAGAGTGACCTTCAAC

Predicted Fragment Size

IR fragment full Seq

0001 GGCAAGGTGA TTGTCAATCT GAAACAAGAC CAAGAAACTG CGAGGAAATA AAACTGCAAT 
0061 ATAAGTGGCT ATAGCGCAAG CCCAGTGGGC GCATCCGCAG ACATGGGTGA GGCGGGTGGC 
0121 AATCCAAAAC CCCAAGAAGC GATCAAAGCA CCAGTACAGC GGGCGGGAGG ACTATATAGC 
0181 CTCCACCACT TACCTCATGG GTTCCGAGCA GGGCAGCAAC TTTAACATGG ACGCCGGCAC 
0241 GTACACCTAC ACTTTTGCTT GCCCCATTCC CAGCCACTGC CCCTCGTCCT TCGAGGGTGC 
0301 CTATGGACAC ATCCGGTATC TGGCCAAGGT CACCTTTTTG AAGCCAGGAG CTTCCAATCG 
0361 CACCCACAAC GTGGGCTTCA CTGTGCTAAA GCTGCTGGAC TTGAACCAGG AGACCAAGAT 
0421 GCTCCGGGAA CCGGCGAGCA ATGAAGCCGT GGAACACTTT TGCCTGATGC ACACGAAGCC 
0481 CGTGCAGTTG AAGGTCACTC TCCAGCA

in silico PCR Fragment

0001 GGCAAGGTGA TTGTCAATCT G-AACAAGAC CAAGAAACTG CGAGG-AATA AAACTGCAA- 
0061 ATAAGTGGCT AT-GCGCAAG CCCAGT-GGC GCATCCGCAG ACAT-GGTAA GGC-GGTGGC 
0121 AATCCAAAAC CCCAAGAAGC GATCAAAGCA CCAGTACAGC GGGCGGGAGG ACTATATAGC 
0181 CTCCACCACT TACCTCATGG GTTCCGAGCA GGGCAGCAAC TTTAACATGG ACGCCGGCAC 
0241 GTACACCTAC ACTTTTGCTT GCCCCATTCC CAGCCACTGC CCCTCGTCCT TCGAGGGTGC 
0301 CTATGGACAC ATCCGGTATC TGGCCAAGGT CACCTTTTTG AAGCCAGGAG CTTCCAATCG 
0361 CACCCACAAC GTGGGCTTCA CTGTGCTAAA GCTGCTGGAC TTGAACCAGG AGACCAAGAT 
0421 GCTCCGGGAA CCGGCGAGCA ATGAAGCCGT GGAACACTTT TGCCTGATGC ACACGAAGCC 
0481 CGTGCAGTTG AAGGTCACTC TCCAGCA

Assemble Data


10086R-7.IR_full       1   GGCAAGGTGATTGTCAATCTGAAACAAGACCAAGAAACTGCGAGGAAATAAAACTGCAAT 60
                           ||||||||||||||||||||| ||||||||||||||||||||||| ||||||||||||| 
10086R-7.in silico     1   GGCAAGGTGATTGTCAATCTG-AACAAGACCAAGAAACTGCGAGG-AATAAAACTGCAA- 60


10086R-7.IR_full       61  ATAAGTGGCTATAGCGCAAGCCCAGTGGGCGCATCCGCAGACATGGGTGAGGCGGGTGGC 120
                           |||||||||||| ||||||||||||| ||||||||||||||||| ||| |||| ||||||
10086R-7.in silico     61  ATAAGTGGCTAT-GCGCAAGCCCAGT-GGCGCATCCGCAGACAT-GGTAAGGC-GGTGGC 120


10086R-7.IR_full       121 AATCCAAAACCCCAAGAAGCGATCAAAGCACCAGTACAGCGGGCGGGAGGACTATATAGC 180
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10086R-7.in silico     121 AATCCAAAACCCCAAGAAGCGATCAAAGCACCAGTACAGCGGGCGGGAGGACTATATAGC 180


10086R-7.IR_full       181 CTCCACCACTTACCTCATGGGTTCCGAGCAGGGCAGCAACTTTAACATGGACGCCGGCAC 240
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10086R-7.in silico     181 CTCCACCACTTACCTCATGGGTTCCGAGCAGGGCAGCAACTTTAACATGGACGCCGGCAC 240


10086R-7.IR_full       241 GTACACCTACACTTTTGCTTGCCCCATTCCCAGCCACTGCCCCTCGTCCTTCGAGGGTGC 300
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10086R-7.in silico     241 GTACACCTACACTTTTGCTTGCCCCATTCCCAGCCACTGCCCCTCGTCCTTCGAGGGTGC 300


10086R-7.IR_full       301 CTATGGACACATCCGGTATCTGGCCAAGGTCACCTTTTTGAAGCCAGGAGCTTCCAATCG 360
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10086R-7.in silico     301 CTATGGACACATCCGGTATCTGGCCAAGGTCACCTTTTTGAAGCCAGGAGCTTCCAATCG 360


10086R-7.IR_full       361 CACCCACAACGTGGGCTTCACTGTGCTAAAGCTGCTGGACTTGAACCAGGAGACCAAGAT 420
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10086R-7.in silico     361 CACCCACAACGTGGGCTTCACTGTGCTAAAGCTGCTGGACTTGAACCAGGAGACCAAGAT 420


10086R-7.IR_full       421 GCTCCGGGAACCGGCGAGCAATGAAGCCGTGGAACACTTTTGCCTGATGCACACGAAGCC 480
                           ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
10086R-7.in silico     421 GCTCCGGGAACCGGCGAGCAATGAAGCCGTGGAACACTTTTGCCTGATGCACACGAAGCC 480


10086R-7.IR_full       481 CGTGCAGTTGAAGGTCACTCTCCAGCA 507
                           |||||||||||||||||||||||||||
10086R-7.in silico     481 CGTGCAGTTGAAGGTCACTCTCCAGCA 507

Off-target information (Last Update: November 4, 2007_NCBI RefSeq Release 26)

Off-target search result

cv : coefficient of variation

(0 mm hits / total siRNA count)

mm : mismatch

cv	0mm	1mm	2mm	3mm	target transcript	off target name
100	482	0	0	0	NM_141479.2	CG10086-RA (CG10086), mRNA
0	0	3	9	8	NM_144393.1	CG18747-RA (CG18747), mRNA
0	0	2	3	6	NM_167833.1	CG1233-RA, transcript variant A (CG1233), mRNA
0	0	2	3	6	NM_138190.1	CG1233-RB, transcript variant B (CG1233), mRNA
0	0	2	2	2	NM_137988.2	CG5543-RA (CG5543), mRNA
0	0	1	3	10	NM_144448.2	CG1915-RC, transcript variant C (sls), mRNA
0	0	1	3	6	NM_175960.3	CG33196-RB (dp), mRNA
0	0	1	3	4	NM_079937.2	CG1915-RA, transcript variant A (sls), mRNA
0	0	1	2	4	NM_001031999.1	CG17117-RF, transcript variant F (hth), mRNA
0	0	0	6	9	NM_142809.2	CG6937-RA (CG6937), mRNA
0	0	0	6	5	NM_057276.3	CG4178-RA (Lsp1beta), mRNA
0	0	0	6	3	NM_144390.1	CG18744-RA (CG18744), mRNA
0	0	0	6	3	NM_080018.1	CG6890-RA (Tollo), mRNA
0	0	0	5	8	NM_130557.2	CG14786-RA (CG14786), mRNA
0	0	0	5	5	NM_079903.2	CG15319-RB (nej), mRNA
0	0	0	5	3	NM_140257.1	CG7283-RA, transcript variant A (RpL10Ab), mRNA
0	0	0	5	3	NM_168480.1	CG7283-RB, transcript variant B (RpL10Ab), mRNA
0	0	0	5	3	NM_142646.1	CG5483-RA (CG5483), mRNA
0	0	0	5	3	NM_143530.2	CG2217-RA (CG2217), mRNA
0	0	0	4	9	NM_078516.2	CG12690-RA (CHES-1-like), mRNA
0	0	0	4	7	NM_143340.2	CG5508-RA, transcript variant A (CG5508), mRNA
0	0	0	4	6	NM_137736.1	CG15666-RA (CG15666), mRNA
0	0	0	4	4	NM_136257.2	CG8677-RA (CG8677), mRNA
0	0	0	4	4	NM_132641.1	CG15743-RA (CG15743), mRNA
0	0	0	4	3	NM_169222.2	CG31462-RA (CG31462), mRNA
0	0	0	3	8	NM_143262.1	CG14254-RA (CG14254), mRNA
0	0	0	3	8	NM_164517.1	CG8817-RC, transcript variant C (lilli), mRNA
0	0	0	3	8	NM_164516.1	CG8817-RA, transcript variant A (lilli), mRNA
0	0	0	3	8	NM_078740.2	CG8817-RB, transcript variant B (lilli), mRNA
0	0	0	3	7	NM_167466.1	CG9012-RB, transcript variant B (Chc), mRNA

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