分離済み変異体

tm6157

Allele Nametm6157
BalanceNot Required
OutCrossNot Accepted
Sequence NameF57C9.7
Gene Nametofu-4
Worm BaseAllele Name tm6157
Gene Name tofu-4
Sequence F57C9.7
Phenotype Information from the receiver is posted in the form of a "researcher : phenotype" homozygous viable.
Mutation site Please see gene structure to locate the deletion in relation to exon(s) 3890/3891-4343/4344 (453 bp deletion)
ChromosomeI
Putative gene structurecomplement(join(3346..3652, 3698..3814, 3859..4091, 4136..4243, 4292..4465, 4511..4810))
Map position-0.72
Balancer
Map position of balancer
Sequence of primersExtFwd:TATGGTTCCCTCGTCGCATT,IntFwd:CTAGTCAGGTCAGGAAGGCT,ExtRev:AGTTTACCGTAACTCAGGTC,IntRev:CCCATGGAAGAAATGTCATG
Distributed lab
DepositorDr. S. Mitani/NBRP
References Please submit your publication
Huang X, Cheng P, Weng C, Xu Z, Zeng C, Xu Z, Chen X, Zhu C, Guang S, Feng X.
A chromodomain protein mediates heterochromatin-directed piRNA expression.
Proc Natl Acad Sci U S A 2021 118(27)  
[ PubMed ID = 34187893 ] [ RRC reference ]

Zeng C, Weng C, Wang X, Yan YH, Li WJ, Xu D, Hong M, Liao S, Dong MQ, Feng X, Xu C, Guang S.
Functional Proteomics Identifies a PICS Complex Required for piRNA Maturation and Chromosome Segregation.
Cell Rep 2019 27(12) 3561-3572.e3 
[ PubMed ID = 31216475 ] [ RRC reference ]

Weng C, Kosalka J, Berkyurek AC, Stempor P, Feng X, Mao H, Zeng C, Li WJ, Yan YH, Dong MQ, Morero NR, Zuliani C, Barabas O, Ahringer J, Guang S, Miska EA.
The USTC co-opts an ancient machinery to drive piRNA transcription in C. elegans.
Genes Dev 2019 33(1-2) 90-102 
[ PubMed ID = 30567997 ] [ RRC reference ]

Goh WS, Seah JW, Harrison EJ, Chen C, Hammell CM, Hannon GJ.
A genome-wide RNAi screen identifies factors required for distinct stages of C. elegans piRNA biogenesis.
Genes Dev 2014 28(7) 797-807 
[ PubMed ID = 24696458 ] [ RRC reference ]