分離済み変異体

tm3039

Allele Nametm3039
BalanceCompleted
OutCrossNot Accepted
Sequence NameF07A11.3
Gene Namenpp-5
Worm BaseAllele Name tm3039 (x1)
Gene Name npp-5
Sequence F07A11.3
Phenotype Information from the receiver is posted in the form of a "researcher : phenotype" lethal or sterile.
Mutation site Please see gene structure to locate the deletion in relation to exon(s) 13192/13193-A-13716/13717 (524 bp deletion + 1 bp insertion)
ChromosomeII
Putative gene structurecomplement(join(10983..11279, 11336..11581, 11763..12211, 12258..13281, 13334..13540, 13589..13701, 13758..13863))
Map position3.78
Balancer,mIn1 [e128 mIs14]
Map position of balancer
Sequence of primersExtRev:GGGGGACAATTCTTCCAACT,IntFwd:TCCAGAGCCGCGTGTTCGAT,ExtFwd:ACGAAAGAGCAGAGCCCATA,IntRev:CAACTCCTTCGACGACTCTA
Distributed lab
DepositorDr. S. Mitani/NBRP
References Please submit your publication
Nkombo Nkoula S, Velez-Aguilera G, Ossareh-Nazari B, Van Hove L, Ayuso C, Legros V, Chevreux G, Thomas L, Seydoux G, Askjaer P, Pintard L.
Mechanisms of nuclear pore complex disassembly by the mitotic Polo-like kinase 1 (PLK-1) in C. elegans embryos.
Sci Adv 2023 9(29) eadf7826 
[ PubMed ID = 37467327 ] [ RRC reference ]

Thomas L, Taleb Ismail B, Askjaer P, Seydoux G.
Nucleoporin foci are stress-sensitive condensates dispensable for C. elegans nuclear pore assembly.
EMBO J 2023 42(13) e112987 
[ PubMed ID = 37254647 ] [ RRC reference ]

Bohr T, Nelson CR, Klee E, Bhalla N.
Spindle assembly checkpoint proteins regulate and monitor meiotic synapsis in C. elegans.
J Cell Biol 2015 211(2) 233-42 
[ PubMed ID = 26483555 ] [ RRC reference ]

Lawrence KS, Chau T, Engebrecht J.
DNA damage response and spindle assembly checkpoint function throughout the cell cycle to ensure genomic integrity.
PLoS Genet 2015 11(4) e1005150 
[ PubMed ID = 25898113 ] [ RRC reference ]

Askjaer P, Galy V, Meister P.
Modern tools to study nuclear pore complexes and nucleocytoplasmic transport in Caenorhabditis elegans.
Methods Cell Biol 2014 122 277-310 
[ PubMed ID = 24857735 ] [ RRC reference ]

Ródenas E, González-Aguilera C, Ayuso C, Askjaer P.
Dissection of the NUP107 nuclear pore subcomplex reveals a novel interaction with spindle assembly checkpoint protein MAD1 in Caenorhabditis elegans.
Mol Biol Cell 2012 23(5) 930-44 
[ PubMed ID = 22238360 ] [ RRC reference ]