分離済み変異体

tm2886

Allele Nametm2886
BalanceNot Required
OutCrossNot Accepted
Sequence NameR06F6.5
Gene Namenpp-19
Worm BaseAllele Name tm2886
Gene Name npp-19
Sequence R06F6.5
Phenotype Information from the receiver is posted in the form of a "researcher : phenotype" homozygous viable.
Mutation site Please see gene structure to locate the deletion in relation to exon(s) 12165/12166-12425/12426 (260 bp deletion)
ChromosomeII
Putative gene structurecomplement(join(11656..11727, 11780..11846, 12026..12210, 12261..12652, 12703..12975, 13025..13098, 13165..13238))
Map position2.98
Balancer
Map position of balancer
Sequence of primersExtFwd:TGATCGGCCATATCGTTCTC,IntFwd:CATGGAAGCAGATCGGTTTG,ExtRev:CAGGCCATGTTCTCGCATCT,IntRev:CATCCGGAAGGCATAACTCA
Distributed lab
DepositorDr. S. Mitani/NBRP
References Please submit your publication
Mossadeq LE, Bellutti L, Borgne RL, Canman JC, Pintard L, Verbavatz JM, Askjaer P, Dumont J.
An interkinetic envelope surrounds chromosomes between meiosis I and II in C. elegans oocytes.
bioRxiv 2024   
[ PubMed ID = 39484525 ] [ RRC reference ]

Mazzetto M, Gonzalez LE, Sanchez N, Reinke V.
Characterization of the distribution and dynamics of chromatin states in the C. elegans germline reveals substantial H3K4me3 remodeling during oogenesis.
Genome Res 2024 34(1) 57-69 
[ PubMed ID = 38164610 ] [ RRC reference ]

Nkombo Nkoula S, Velez-Aguilera G, Ossareh-Nazari B, Van Hove L, Ayuso C, Legros V, Chevreux G, Thomas L, Seydoux G, Askjaer P, Pintard L.
Mechanisms of nuclear pore complex disassembly by the mitotic Polo-like kinase 1 (PLK-1) in C. elegans embryos.
Sci Adv 2023 9(29) eadf7826 
[ PubMed ID = 37467327 ] [ RRC reference ]

Mauro MS, Celma G, Zimyanin V, Magaj MM, Gibson KH, Redemann S, Bahmanyar S.
Ndc1 drives nuclear pore complex assembly independent of membrane biogenesis to promote nuclear formation and growth.
Elife 2022 11  
[ PubMed ID = 35852146 ] [ RRC reference ]

Askjaer P, Galy V, Meister P.
Modern tools to study nuclear pore complexes and nucleocytoplasmic transport in Caenorhabditis elegans.
Methods Cell Biol 2014 122 277-310 
[ PubMed ID = 24857735 ] [ RRC reference ]

Ródenas E, Klerkx EP, Ayuso C, Audhya A, Askjaer P.
Early embryonic requirement for nucleoporin Nup35/NPP-19 in nuclear assembly.
Dev Biol 2009 327(2) 399-409 
[ PubMed ID = 19146848 ] [ RRC reference ]