Home > 分離済み変異体 > tm2649

分離済み変異体

Allele Name		tm2649
Balance		Not Required
OutCross		Not Accepted
Sequence Name		R06C7.7
Gene Name		lin-61
Worm Base	Allele Name	tm2649
	Gene Name	lin-61
	Sequence	R06C7.7
Phenotype Information from the receiver is posted in the form of a "researcher : phenotype"		homozygous viable
Mutation site Please see gene structure to locate the deletion in relation to exon(s)		17467/17468-18139/18140 (672 bp deletion)
Chromosome		I
Putative gene structure		complement(join(16631..16709, 16764..16903, 16991..17800, 17851..17947, 18074..18240, 18289..18471))
Map position		1.86
Balancer
Map position of balancer
Sequence of primers		ExtFwd:CAAGTCAAGCGGTAGGGTTT,IntFwd:GCGGTAGGGTTTCTGCAGAT,ExtRev:CGAATGCGTGAAGGAAGGAG,IntRev:ACTGCGATTCGATTCAAGTC
Distributed lab
Depositor		Dr. S. Mitani/NBRP
References		Please submit your publication Mazzetto M, Gonzalez LE, Sanchez N, Reinke V. Characterization of the distribution and dynamics of chromatin states in the C. elegans germline reveals substantial H3K4me3 remodeling during oogenesis. Genome Res 2024 34(1) 57-69  [ PubMed ID = 38164610 ] [ RRC reference ] Delaney CE, Methot SP, Kalck V, Seebacher J, Hess D, Gasser SM, Padeken J. SETDB1-like MET-2 promotes transcriptional silencing and development independently of its H3K9me-associated catalytic activity. Nat Struct Mol Biol 2022 29(2) 85-96  [ PubMed ID = 35102319 ] [ RRC reference ] Padeken J, Methot S, Zeller P, Delaney CE, Kalck V, Gasser SM. Argonaute NRDE-3 and MBT domain protein LIN-61 redundantly recruit an H3K9me3 HMT to prevent embryonic lethality and transposon expression. Genes Dev 2021 35(1-2) 82-101  [ PubMed ID = 33303642 ] [ RRC reference ] McMurchy AN, Stempor P, Gaarenstroom T, Wysolmerski B, Dong Y, Aussianikava D, Appert A, Huang N, Kolasinska-Zwierz P, Sapetschnig A, Miska EA, Ahringer J. A team of heterochromatin factors collaborates with small RNA pathways to combat repetitive elements and germline stress. Elife 2017 6   [ PubMed ID = 28294943 ] [ RRC reference ] Johnson NM, Lemmens BB, Tijsterman M. A role for the malignant brain tumour (MBT) domain protein LIN-61 in DNA double-strand break repair by homologous recombination. PLoS Genet 2013 9(3) e1003339  [ PubMed ID = 23505385 ] [ RRC reference ] Wu X, Shi Z, Cui M, Han M, Ruvkun G. Repression of germline RNAi pathways in somatic cells by retinoblastoma pathway chromatin complexes. PLoS Genet 2012 8(3) e1002542  [ PubMed ID = 22412383 ] [ RRC reference ] Koester-Eiserfunke N, Fischle W. H3K9me2/3 binding of the MBT domain protein LIN-61 is essential for Caenorhabditis elegans vulva development. PLoS Genet 2011 7(3) e1002017  [ PubMed ID = 21437264 ] [ RRC reference ]