分離済み変異体

tm2526

Allele Name

tm2526

Balance

Not Required

OutCross

Not Accepted

Sequence Name

D1037.4

Gene Name

rab-8

Worm Base

Allele Name

tm2526

Gene Name

rab-8

Sequence

D1037.4

Phenotype

homozygous viable. Dr. O. Blacque: dye-filling normal. Dr. K. Sato: Nature448, 366 (2007). Dr. S. Eimer: normal levamisole receptor trafficking. Dr. H.C. Korswagen: no QL migration defect or ALM/PLM polarity defects.

Mutation site

22868/22869-23109/23110 (241 bp deletion)

Chromosome

Putative gene structure

complement(join(22559..22948, 23008..23068, 23116..23270, 23318..23347))

Map position

-2.95

Balancer

Map position of balancer

Sequence of primers

IntFwd:CTTCTTCTGTGTCCCCGATC,ExtFwd:TATGCGAGAGAGAGCGGACA,IntRev:CTTTTGCATGCCAAGATGTC,ExtRev:CCGGGACCCATCAGTGTCAA

Distributed lab

Depositor

Dr. S. Mitani/NBRP

References

Please submit your publication

Li X, Liu B, Wen Y, Wang J, Guo YR, Shi A, Lin L.
Coordination of RAB-8 and RAB-11 during unconventional protein secretion.
J Cell Biol 2024 223(2)
[ PubMed ID = 38019180 ] [ RRC reference ]

Wang X, Li X, Wang J, Wang J, Hu C, Zeng J, Shi A, Lin L.
SMGL-1/NBAS acts as a RAB-8 GEF to regulate unconventional protein secretion.
J Cell Biol 2022 221(7)
[ PubMed ID = 35604368 ] [ RRC reference ]

Lin-Moore AT, Oyeyemi MJ, Hammarlund M.
rab-27 acts in an intestinal pathway to inhibit axon regeneration in C. elegans.
PLoS Genet 2021 17(11) e1009877
[ PubMed ID = 34818334 ] [ RRC reference ]

Choi J, Sung JY, Lee S, Yoo J, Rongo C, Kim YN, Shim J.
Rab8 and Rabin8-Mediated Tumor Formation by Hyperactivated EGFR Signaling via FGFR Signaling.
Int J Mol Sci 2020 21(20)
[ PubMed ID = 33092268 ] [ RRC reference ]

Androwski RJ, Asad N, Wood JG, Hofer A, Locke S, Smith CM, Rose B, Schroeder NE.
Mutually exclusive dendritic arbors in C. elegans neurons share a common architecture and convergent molecular cues.
PLoS Genet 2020 16(9) e1009029
[ PubMed ID = 32997655 ] [ RRC reference ]

Gao J, Zhao L, Luo Q, Liu S, Lin Z, Wang P, Fu X, Chen J, Zhang H, Lin L, Shi A.
An EHBP-1-SID-3-DYN-1 axis promotes membranous tubule fission during endocytic recycling.
PLoS Genet 2020 16(5) e1008763
[ PubMed ID = 32384077 ] [ RRC reference ]

Chen D, Xu W, Wang Y, Ye Y, Wang Y, Yu M, Gao J, Wei J, Dong Y, Zhang H, Fu X, Ma K, Wang H, Yang Z, Zhou J, Cheng W, Wang S, Chen J, Grant BD, Myers CL, Shi A, Xia T.
Revealing Functional Crosstalk between Distinct Bioprocesses through Reciprocal Functional Tests of Genetically Interacting Genes.
Cell Rep 2019 29(9) 2646-2658.e5
[ PubMed ID = 31775035 ] [ RRC reference ]

Laurent P, Ch'ng Q, Jospin M, Chen C, Lorenzo R, de Bono M.
Genetic dissection of neuropeptide cell biology at high and low activity in a defined sensory neuron.
Proc Natl Acad Sci U S A 2018 115(29) E6890-E6899
[ PubMed ID = 29959203 ] [ RRC reference ]

Zou W, Yadav S, DeVault L, Nung Jan Y, Sherwood DR.
RAB-10-Dependent Membrane Transport Is Required for Dendrite Arborization.
PLoS Genet 2015 11(9) e1005484
[ PubMed ID = 26394140 ] [ RRC reference ]

Brear AG, Yoon J, Wojtyniak M, Sengupta P.
Diverse cell type-specific mechanisms localize G protein-coupled receptors to Caenorhabditis elegans sensory cilia.
Genetics 2014 197(2) 667-84
[ PubMed ID = 24646679 ] [ RRC reference ]

Wojtyniak M, Brear AG, O'Halloran DM, Sengupta P.
Cell- and subunit-specific mechanisms of CNG channel ciliary trafficking and localization in C. elegans.
J Cell Sci 2013 126(Pt 19) 4381-95
[ PubMed ID = 23886944 ] [ RRC reference ]

Olivier-Mason A, Wojtyniak M, Bowie RV, Nechipurenko IV, Blacque OE, Sengupta P.
Transmembrane protein OSTA-1 shapes sensory cilia morphology via regulation of intracellular membrane trafficking in C. elegans.
Development 2013 140(7) 1560-72
[ PubMed ID = 23482491 ] [ RRC reference ]

Shi A, Liu O, Koenig S, Banerjee R, Chen CC, Eimer S, Grant BD.
RAB-10-GTPase-mediated regulation of endosomal phosphatidylinositol-4,5-bisphosphate.
Proc Natl Acad Sci U S A 2012 109(35) E2306-15
[ PubMed ID = 22869721 ] [ RRC reference ]

Kaplan OI, Doroquez DB, Cevik S, Bowie RV, Clarke L, Sanders AA, Kida K, Rappoport JZ, Sengupta P, Blacque OE.
Endocytosis genes facilitate protein and membrane transport in C. elegans sensory cilia.
Curr Biol 2012 22(6) 451-60
[ PubMed ID = 22342749 ] [ RRC reference ]

Kaplan OI, Molla-Herman A, Cevik S, Ghossoub R, Kida K, Kimura Y, Jenkins P, Martens JR, Setou M, Benmerah A, Blacque OE.
The AP-1 clathrin adaptor facilitates cilium formation and functions with RAB-8 in C. elegans ciliary membrane transport.
J Cell Sci 2010 123(Pt 22) 3966-77
[ PubMed ID = 20980383 ] [ RRC reference ]

Mukhopadhyay S, Lu Y, Shaham S, Sengupta P.
Sensory signaling-dependent remodeling of olfactory cilia architecture in C. elegans.
Dev Cell 2008 14(5) 762-74
[ PubMed ID = 18477458 ] [ RRC reference ]

Sato T, Mushiake S, Kato Y, Sato K, Sato M, Takeda N, Ozono K, Miki K, Kubo Y, Tsuji A, Harada R, Harada A.
The Rab8 GTPase regulates apical protein localization in intestinal cells.
Nature 2007 448(7151) 366-9
[ PubMed ID = 17597763 ] [ RRC reference ]