分離済み変異体

tm1814

Allele Name

tm1814

Balance

Not Required

OutCross

Not Accepted

Sequence Name

R08C7.10

Gene Name

wapl-1

Worm Base

Allele Name

tm1814

Gene Name

wapl-1

Sequence

R08C7.10

Phenotype

homozygous viable.

Mutation site

24298/24299-NG-24844/24845 (546 bp deletion + 2 bp insertion)

Chromosome

Putative gene structure

complement(join(21564..21638, 21690..22022, 22355..22480, 22530..22604, 22659..22890, 22939..23015, 23065..23153, 23205..23674, 23720..23913, 23960..24114, 24164..24356, 24402..24582, 24638..24678))

Map position

0.26

Balancer

Map position of balancer

Sequence of primers

IntFwd:TCCTTGTCCCCCTAGACGGA,ExtRev:CCACTCACTTGCGTCGACGT,IntRev:CTCTCGGCGGCATTCTAAAC,ExtFwd:CTCTCACCAGCCGGATCGTA

Distributed lab

Depositor

Dr. S. Mitani/NBRP

References

Please submit your publication

Castellano-Pozo M, Sioutas G, Barroso C, Prince JP, Lopez-Jimenez P, Davy J, Jaso-Tamame AL, Crawley O, Shao N, Page J, Martinez-Perez E.
The kleisin subunit controls the function of C. elegans meiotic cohesins by determining the mode of DNA binding and differential regulation by SCC-2 and WAPL-1.
Elife 2023 12
[ PubMed ID = 37650378 ] [ RRC reference ]

Woglar A, Yamaya K, Roelens B, Boettiger A, Köhler S, Villeneuve AM.
Quantitative cytogenetics reveals molecular stoichiometry and longitudinal organization of meiotic chromosome axes and loops.
PLoS Biol 2020 18(8) e3000817
[ PubMed ID = 32813728 ] [ RRC reference ]

Hernandez MR, Davis MB, Jiang J, Brouhard EA, Severson AF, Csankovszki G.
Condensin I protects meiotic cohesin from WAPL-1 mediated removal.
PLoS Genet 2018 14(5) e1007382
[ PubMed ID = 29768402 ] [ RRC reference ]

Crawley O, Barroso C, Testori S, Ferrandiz N, Silva N, Castellano-Pozo M, Jaso-Tamame AL, Martinez-Perez E.
Cohesin-interacting protein WAPL-1 regulates meiotic chromosome structure and cohesion by antagonizing specific cohesin complexes.
Elife 2016 5 e10851
[ PubMed ID = 26841696 ] [ RRC reference ]