分離済み変異体

tm1741

Allele Name

tm1741

Balance

Completed

OutCross

Not Accepted

Sequence Name

F36H2.1

Gene Name

tat-5

Worm Base

Allele Name

tm1741 (x1)

Gene Name

tat-5

Sequence

F36H2.1

Phenotype

lethal or sterile. Dr. M. Han: Let and Ste.

Mutation site

4254/4255-4646/4647 (392 bp deletion)

Chromosome

Putative gene structure

complement(join(AL021466.1:5183..5371, 129..277, 324..580, 1366..1940, 2420..3017, 3365..3701, 4144..4390, 4445..4954, 5355..5514, 6300..6502))

Map position

3.71

Balancer

hT2 [bli-4(e937) let-? qIs48]

Map position of balancer

Sequence of primers

IntFwd:CGACCAATTTGCCACGAGTA,ExtFwd:CGGAATATGCTTGTCACGAC,IntRev:GGCGACGTGTTCTGCACTCA,ExtRev:CCGAACTGCGCCAGCATTAT

Distributed lab

Depositor

Dr. S. Mitani/NBRP

References

Please submit your publication

Maniates KA, Suryanarayanan S, Rumin A, Lewin M, Singson A, Wehman AM.
Sperm activation for fertilization requires robust activity of the TAT-5 lipid flippase.
Dev Biol 2025 528 66-78
[ PubMed ID = 40915529 ] [ RRC reference ]

Park S, Noblett N, Pitts L, Colavita A, Wehman AM, Jin Y, Chisholm AD.
Dopey-dependent regulation of extracellular vesicles maintains neuronal morphology.
Curr Biol 2024 34(21) 4920-4933.e11
[ PubMed ID = 39378880 ] [ RRC reference ]

Park S, Noblett N, Pitts L, Colavita A, Wehman AM, Jin Y, Chisholm AD.
Dopey-dependent regulation of extracellular vesicles maintains neuronal morphology.
bioRxiv 2024
[ PubMed ID = 38766017 ] [ RRC reference ]

Pitts LR, Nguyen AT, Wehman AM.
Conserved N- and C-terminal motifs of PAD-1 are required to inhibit extracellular vesicle release.
MicroPubl Biol 2022 2022
[ PubMed ID = 36188098 ] [ RRC reference ]

C. elegans Deletion Mutant Consortium.
large-scale screening for targeted knockouts in the Caenorhabditis elegans genome.
G3 (Bethesda) 2012 2(11) 1415-25
[ PubMed ID = 23173093 ] [ RRC reference ]

Wehman AM, Poggioli C, Schweinsberg P, Grant BD, Nance J.
The P4-ATPase TAT-5 inhibits the budding of extracellular vesicles in C. elegans embryos.
Curr Biol 2011 21(23) 1951-9
[ PubMed ID = 22100064 ] [ RRC reference ]

Lyssenko NN, Miteva Y, Gilroy S, Hanna-Rose W, Schlegel RA.
An unexpectedly high degree of specialization and a widespread involvement in sterol metabolism among the C. elegans putative aminophospholipid translocases.
BMC Dev Biol 2008 8 96
[ PubMed ID = 18831765 ] [ RRC reference ]